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The map label for this gene is pdxB

Identifier: 77460107

GI number: 77460107

Start: 4395183

End: 4396325

Strand: Reverse

Name: pdxB

Synonym: Pfl01_3886

Alternate gene names: 77460107

Gene position: 4396325-4395183 (Counterclockwise)

Preceding gene: 255961267

Following gene: 77460105

Centisome position: 68.28

GC content: 63.52

Gene sequence:

>1143_bases
ATGCTGATTGTTGCCGACGAAAATATCCCGCTGCTCGATGCCTTTTTTGCCGGTTTCGGTGAGATTCGCCGAGTACCGGG
CCGTTCCATCGACCGGGCCACGGTCGAACAGGCCGATGTATTGCTGGTGCGCTCGGTGACCAACGTCAACCGCGCACTGC
TTGAAGGCAGCAAGGTGCGTTTTGTCGGCACCTGCACCATCGGCACCGATCATCTGGATCTCGATTACTTCAATGAGGCC
GGGATCACCTGGTCCAGCGCGCCCGGCTGCAATGCCCGGGGCGTGGTCGATTACGTGCTCGGCAGCCTGATGACCCTGGC
TGAAATCGAAGGCGTCGATTTGCGTGAACGCACTTACGGTGTGATCGGGGCCGGTGAAGTGGGCGGTCGCCTGATCAAGG
TGCTCAAAGGCCTGGGCTGGAACGTGAAAGTCTGCGATCCGCCGCGTCAGGCGGCCGAGGGCGGCGACTATGTCAGCCTG
GAGCAGATCATCGCGCAGTGCGACGTGATCAGCCTGCACACCCCGCTGACCCGCAGCGGTGACGGTGCGACCTGGCATTT
GTTCGACGAGCAGCGTTTGCAGCAGCTCAAGCCCGGCGCCTGGTTGATCAACGCGGCTCGCGGTCCGGTGGTGGATAACA
CCGCGTTGCGCGAAGTGCTGCTGGAGCGTGAAGACCTGCAAGCAGTGCTCGATGTCTGGGAGGCCGAGCCGCAAGTCGAT
GTTTCTCTGGCCGAGCTCTGCGTGTTGGCGACGCCGCACATCGCCGGTTACAGCCTCGACGGCAAGCAGCGCGGCACCGC
GCAGATCTATCAGGCTTACTGCGACTTTATCGGTGAGCCCGCCAGCATTCAGCTCGGCGATCTGCTGCCGGCGCCGTGGT
TGTCGGAAGTTTCCCTGCATGCCGACAGCGATCCAGCCTGGGCGCTGGCGATGTTGTGCCGCGGTGTGTACGACCCGCGC
CGGGACGACGCGGATTTCCGTCGCACGCTGCTGGGCAGCGTCGGTGAGCAACGTGCGGCGTTCGATGTGTTGCGCAAGCA
GTATCCGTCGCGACGCGAGATCGAAGGTTTGAAGGTGCGGATCGAGGGCGATTCGCCGGCCCTGCGCCAGATCGTGGCCG
CGCTGGGCGCTGTGGCGGTATAA

Upstream 100 bases:

>100_bases
TTGCGGATTTGTAATCGCCGCGCCTCATCAAGAAATCAAAGCCTGCTGGCTCATCGCAGTCCCAACGCCTACACTGCCGA
TCCGCCCAAGGAGCCCCGCC

Downstream 100 bases:

>100_bases
ACAGCGGGCAGAAAAAACCCGGCCTGCAGGGGCCGGGTCAGGAAGACTGTGGCGATATCACTTTTGTTCGGCAGGCTTGA
CCAATCGCTGTTCCAGTTCG

Product: erythronate-4-phosphate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MLIVADENIPLLDAFFAGFGEIRRVPGRSIDRATVEQADVLLVRSVTNVNRALLEGSKVRFVGTCTIGTDHLDLDYFNEA
GITWSSAPGCNARGVVDYVLGSLMTLAEIEGVDLRERTYGVIGAGEVGGRLIKVLKGLGWNVKVCDPPRQAAEGGDYVSL
EQIIAQCDVISLHTPLTRSGDGATWHLFDEQRLQQLKPGAWLINAARGPVVDNTALREVLLEREDLQAVLDVWEAEPQVD
VSLAELCVLATPHIAGYSLDGKQRGTAQIYQAYCDFIGEPASIQLGDLLPAPWLSEVSLHADSDPAWALAMLCRGVYDPR
RDDADFRRTLLGSVGEQRAAFDVLRKQYPSRREIEGLKVRIEGDSPALRQIVAALGAVAV

Sequences:

>Translated_380_residues
MLIVADENIPLLDAFFAGFGEIRRVPGRSIDRATVEQADVLLVRSVTNVNRALLEGSKVRFVGTCTIGTDHLDLDYFNEA
GITWSSAPGCNARGVVDYVLGSLMTLAEIEGVDLRERTYGVIGAGEVGGRLIKVLKGLGWNVKVCDPPRQAAEGGDYVSL
EQIIAQCDVISLHTPLTRSGDGATWHLFDEQRLQQLKPGAWLINAARGPVVDNTALREVLLEREDLQAVLDVWEAEPQVD
VSLAELCVLATPHIAGYSLDGKQRGTAQIYQAYCDFIGEPASIQLGDLLPAPWLSEVSLHADSDPAWALAMLCRGVYDPR
RDDADFRRTLLGSVGEQRAAFDVLRKQYPSRREIEGLKVRIEGDSPALRQIVAALGAVAV
>Mature_380_residues
MLIVADENIPLLDAFFAGFGEIRRVPGRSIDRATVEQADVLLVRSVTNVNRALLEGSKVRFVGTCTIGTDHLDLDYFNEA
GITWSSAPGCNARGVVDYVLGSLMTLAEIEGVDLRERTYGVIGAGEVGGRLIKVLKGLGWNVKVCDPPRQAAEGGDYVSL
EQIIAQCDVISLHTPLTRSGDGATWHLFDEQRLQQLKPGAWLINAARGPVVDNTALREVLLEREDLQAVLDVWEAEPQVD
VSLAELCVLATPHIAGYSLDGKQRGTAQIYQAYCDFIGEPASIQLGDLLPAPWLSEVSLHADSDPAWALAMLCRGVYDPR
RDDADFRRTLLGSVGEQRAAFDVLRKQYPSRREIEGLKVRIEGDSPALRQIVAALGAVAV

Specific function: Catalyzes the oxidation of erythronate-4-phosphate to 3- hydroxy-2-oxo-4-phosphonooxybutanoate

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily

Homologues:

Organism=Homo sapiens, GI23308577, Length=269, Percent_Identity=26.7657992565056, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI61743967, Length=257, Percent_Identity=26.8482490272374, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4557497, Length=257, Percent_Identity=26.8482490272374, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI145580578, Length=284, Percent_Identity=25.7042253521127, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI4557499, Length=284, Percent_Identity=25.7042253521127, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI145580575, Length=284, Percent_Identity=25.7042253521127, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI6912396, Length=295, Percent_Identity=26.1016949152542, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788660, Length=381, Percent_Identity=47.244094488189, Blast_Score=307, Evalue=8e-85,
Organism=Escherichia coli, GI1787645, Length=204, Percent_Identity=29.4117647058824, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI87082289, Length=257, Percent_Identity=30.3501945525292, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789279, Length=260, Percent_Identity=29.2307692307692, Blast_Score=89, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17532191, Length=261, Percent_Identity=28.735632183908, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320925, Length=276, Percent_Identity=26.0869565217391, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6322116, Length=309, Percent_Identity=25.2427184466019, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324055, Length=254, Percent_Identity=26.3779527559055, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6324964, Length=303, Percent_Identity=25.0825082508251, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24646446, Length=227, Percent_Identity=28.1938325991189, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24646448, Length=227, Percent_Identity=28.1938325991189, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24646452, Length=227, Percent_Identity=28.1938325991189, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24646450, Length=227, Percent_Identity=28.1938325991189, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI62472511, Length=227, Percent_Identity=28.1938325991189, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19921140, Length=288, Percent_Identity=25.3472222222222, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI28574286, Length=232, Percent_Identity=25.4310344827586, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXB_PSEPF (Q3K9D1)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_349614.1
- ProteinModelPortal:   Q3K9D1
- SMR:   Q3K9D1
- STRING:   Q3K9D1
- GeneID:   3712532
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_3886
- eggNOG:   COG0111
- HOGENOM:   HBG289972
- OMA:   SAPGCNA
- ProtClustDB:   PRK00257
- BioCyc:   PFLU205922:PFL_3886-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01825
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR020921
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10996:SF4

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C

EC number: =1.1.1.290

Molecular weight: Translated: 41370; Mature: 41370

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1; PS00670 D_2_HYDROXYACID_DH_2; PS00671 D_2_HYDROXYACID_DH_3

Important sites: ACT_SITE 207-207 ACT_SITE 236-236 ACT_SITE 253-253 BINDING 45-45 BINDING 66-66 BINDING 146-146 BINDING 174-174 BINDING 231-231 BINDING 256-256 BINDING 257-257

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIVADENIPLLDAFFAGFGEIRRVPGRSIDRATVEQADVLLVRSVTNVNRALLEGSKVR
CEEEECCCCCHHHHHHHCHHHHHCCCCCCCCHHHHHHHHEEEEHHHHHHHHHHHCCCCEE
FVGTCTIGTDHLDLDYFNEAGITWSSAPGCNARGVVDYVLGSLMTLAEIEGVDLRERTYG
EEEEEEECCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCC
VIGAGEVGGRLIKVLKGLGWNVKVCDPPRQAAEGGDYVSLEQIIAQCDVISLHTPLTRSG
EEECCHHHHHHHHHHHCCCCEEEECCCCHHHHCCCCEEHHHHHHHHCCEEEECCCCCCCC
DGATWHLFDEQRLQQLKPGAWLINAARGPVVDNTALREVLLEREDLQAVLDVWEAEPQVD
CCCEEEEECHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VSLAELCVLATPHIAGYSLDGKQRGTAQIYQAYCDFIGEPASIQLGDLLPAPWLSEVSLH
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHEEEE
ADSDPAWALAMLCRGVYDPRRDDADFRRTLLGSVGEQRAAFDVLRKQYPSRREIEGLKVR
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCEEEE
IEGDSPALRQIVAALGAVAV
ECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLIVADENIPLLDAFFAGFGEIRRVPGRSIDRATVEQADVLLVRSVTNVNRALLEGSKVR
CEEEECCCCCHHHHHHHCHHHHHCCCCCCCCHHHHHHHHEEEEHHHHHHHHHHHCCCCEE
FVGTCTIGTDHLDLDYFNEAGITWSSAPGCNARGVVDYVLGSLMTLAEIEGVDLRERTYG
EEEEEEECCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCC
VIGAGEVGGRLIKVLKGLGWNVKVCDPPRQAAEGGDYVSLEQIIAQCDVISLHTPLTRSG
EEECCHHHHHHHHHHHCCCCEEEECCCCHHHHCCCCEEHHHHHHHHCCEEEECCCCCCCC
DGATWHLFDEQRLQQLKPGAWLINAARGPVVDNTALREVLLEREDLQAVLDVWEAEPQVD
CCCEEEEECHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VSLAELCVLATPHIAGYSLDGKQRGTAQIYQAYCDFIGEPASIQLGDLLPAPWLSEVSLH
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHEEEE
ADSDPAWALAMLCRGVYDPRRDDADFRRTLLGSVGEQRAAFDVLRKQYPSRREIEGLKVR
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCEEEE
IEGDSPALRQIVAALGAVAV
ECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA