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The map label for this gene is wcaJ [H]

Identifier: 77460051

GI number: 77460051

Start: 4329199

End: 4330593

Strand: Direct

Name: wcaJ [H]

Synonym: Pfl01_3829

Alternate gene names: 77460051

Gene position: 4329199-4330593 (Clockwise)

Preceding gene: 77460050

Following gene: 77460057

Centisome position: 67.24

GC content: 60.0

Gene sequence:

>1395_bases
ATGGATCTTTCTCTTCGTATCAATCGAAACACCGGCCTCAAGGGACTGACCTTTTGGGGCCAATGGGCGCTGGCACAGAG
TTTCATTGTTTCCCTGTTGTTCATACTGGCCGAACAGCACACGGGCACCGTCGAGTTCTACTATCGGATGTGCACGATCC
TGGCGGTACTGGCTTCGGTGCCGGCTTATACCTTCAGCGGCGCGTATCGAAAGCGAGACAATTACCTGACCGGGCTGGGT
CGACTGTTCATGGGCTGGTTCATGACCATGGCCGGGCTGGCGTTCATCGCCTTCATCTGCAAGGCCGATGCGCTGTTCTC
CCGCCAGGTCATCCTTGAGTGGGCGGTGTACGGTTTCCTCGGTCAGGCGCTGCTGTACGCGCCGCTGCATGCGTTCTCGA
AGTTCTACCAGCGGTCGCGCAAAAGCGAACACAAGACCCTGATCGTCGGCACCGGCGAACTGGCACTGGGCCTGGCGAAG
AAATTGAGCCAGCATGAAAACCTGCCGCTGGTTGGTCTGGTCAGCAACGGCGATACCCCGTCGCTGGAAGCGGACGCCCC
ACGCGTCGTCGGCGCTCAGGACGAACTGCTTGAGCTGATCCAGACCCACGACATCCGCCGCTTGTACATCACCCTGCCGC
TGTCGGAAGCCGCCAAGATCGAAGCCATGTACGTCGATCTGCTGGATGCCAACGTTGACGTGGTCTGGGTGCCGGACCTG
AACAGCCTGACGCTGCTCAACCACTCGGTGAAAGTCGTGGACGGTCTGCCGGCGATCTACCTCAACGAAAGCCCGTTGAC
CAGCCGCCCGACTGCTGCGCTGAGCAAGAGCCTGGTGGAAAAAGCCGTCGCCCTGCTGGCCATAATTCTGTTGAGCCCGG
TGCTGCTGGCCGTGGCGCTGGCGGTGAAGCTCACCTCGCCGGGTCCGGTGTTCTTCAAGCAGGATCGCCACGGCTGGAAC
GGCAAGGTGATCAAGGTCTGGAAATTCCGCTCGATGCGCGTGCACGATGACCGTGAGGTCAAACAGGCCAGCCGCAACGA
TTCGCGCATCACGCCAGTCGGTCGCTTCATCCGTCGCACTTCGCTGGACGAGCTGCCGCAACTGTTCAACGTGCTGCAAG
GCCACATGGCGCTGGTCGGCCCACGCCCGCACGCCGTGGCGCACAACAACTACTACTCGGGCAAGATCCTCGCCTACATG
GCCCGTCACCGAATCAAACCGGGCATTACTGGCCTGGCGCAGATCAGCGGTTGCCGGGGTGAGACAGACACCCTCGACAA
GATGGAAAAGCGTGTTGAGATCGACCTGAAGTACATCAATAACTGGTCGCTGTGGCTGGATGTGAAGATCCTGGTGAAGA
CGCCGTTCACCCTGCTGTCGAAGGATATTTACTGA

Upstream 100 bases:

>100_bases
TGTTACCTCAGGACACTGGCTCAAACAACAATAACAAGTCTGCTTAACAACTCGGTTTTCTACTGTCGAAACCTGAATGG
ACGTCTTTTGGGGATATCGT

Downstream 100 bases:

>100_bases
GACACAGCGCTTGAGAAGGCAACACCGCAAGGGGACGAAAGTCCCCTTTTTTGTGGGCGGGATTTGGGGGTTGGCGGAGG
GCTCTGCGGCCAAGGAGAGT

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MDLSLRINRNTGLKGLTFWGQWALAQSFIVSLLFILAEQHTGTVEFYYRMCTILAVLASVPAYTFSGAYRKRDNYLTGLG
RLFMGWFMTMAGLAFIAFICKADALFSRQVILEWAVYGFLGQALLYAPLHAFSKFYQRSRKSEHKTLIVGTGELALGLAK
KLSQHENLPLVGLVSNGDTPSLEADAPRVVGAQDELLELIQTHDIRRLYITLPLSEAAKIEAMYVDLLDANVDVVWVPDL
NSLTLLNHSVKVVDGLPAIYLNESPLTSRPTAALSKSLVEKAVALLAIILLSPVLLAVALAVKLTSPGPVFFKQDRHGWN
GKVIKVWKFRSMRVHDDREVKQASRNDSRITPVGRFIRRTSLDELPQLFNVLQGHMALVGPRPHAVAHNNYYSGKILAYM
ARHRIKPGITGLAQISGCRGETDTLDKMEKRVEIDLKYINNWSLWLDVKILVKTPFTLLSKDIY

Sequences:

>Translated_464_residues
MDLSLRINRNTGLKGLTFWGQWALAQSFIVSLLFILAEQHTGTVEFYYRMCTILAVLASVPAYTFSGAYRKRDNYLTGLG
RLFMGWFMTMAGLAFIAFICKADALFSRQVILEWAVYGFLGQALLYAPLHAFSKFYQRSRKSEHKTLIVGTGELALGLAK
KLSQHENLPLVGLVSNGDTPSLEADAPRVVGAQDELLELIQTHDIRRLYITLPLSEAAKIEAMYVDLLDANVDVVWVPDL
NSLTLLNHSVKVVDGLPAIYLNESPLTSRPTAALSKSLVEKAVALLAIILLSPVLLAVALAVKLTSPGPVFFKQDRHGWN
GKVIKVWKFRSMRVHDDREVKQASRNDSRITPVGRFIRRTSLDELPQLFNVLQGHMALVGPRPHAVAHNNYYSGKILAYM
ARHRIKPGITGLAQISGCRGETDTLDKMEKRVEIDLKYINNWSLWLDVKILVKTPFTLLSKDIY
>Mature_464_residues
MDLSLRINRNTGLKGLTFWGQWALAQSFIVSLLFILAEQHTGTVEFYYRMCTILAVLASVPAYTFSGAYRKRDNYLTGLG
RLFMGWFMTMAGLAFIAFICKADALFSRQVILEWAVYGFLGQALLYAPLHAFSKFYQRSRKSEHKTLIVGTGELALGLAK
KLSQHENLPLVGLVSNGDTPSLEADAPRVVGAQDELLELIQTHDIRRLYITLPLSEAAKIEAMYVDLLDANVDVVWVPDL
NSLTLLNHSVKVVDGLPAIYLNESPLTSRPTAALSKSLVEKAVALLAIILLSPVLLAVALAVKLTSPGPVFFKQDRHGWN
GKVIKVWKFRSMRVHDDREVKQASRNDSRITPVGRFIRRTSLDELPQLFNVLQGHMALVGPRPHAVAHNNYYSGKILAYM
ARHRIKPGITGLAQISGCRGETDTLDKMEKRVEIDLKYINNWSLWLDVKILVKTPFTLLSKDIY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=316, Percent_Identity=45.253164556962, Blast_Score=275, Evalue=4e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 51965; Mature: 51965

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLSLRINRNTGLKGLTFWGQWALAQSFIVSLLFILAEQHTGTVEFYYRMCTILAVLASV
CCEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
PAYTFSGAYRKRDNYLTGLGRLFMGWFMTMAGLAFIAFICKADALFSRQVILEWAVYGFL
CCHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GQALLYAPLHAFSKFYQRSRKSEHKTLIVGTGELALGLAKKLSQHENLPLVGLVSNGDTP
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCC
SLEADAPRVVGAQDELLELIQTHDIRRLYITLPLSEAAKIEAMYVDLLDANVDVVWVPDL
CCCCCCCCEECCHHHHHHHHHHCCCEEEEEEEECCCCHHHHEEEEEEEECCCCEEECCCC
NSLTLLNHSVKVVDGLPAIYLNESPLTSRPTAALSKSLVEKAVALLAIILLSPVLLAVAL
CCEEEECCCEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVKLTSPGPVFFKQDRHGWNGKVIKVWKFRSMRVHDDREVKQASRNDSRITPVGRFIRRT
HHEECCCCCEEEECCCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCCCCCCHHHHHHHHC
SLDELPQLFNVLQGHMALVGPRPHAVAHNNYYSGKILAYMARHRIKPGITGLAQISGCRG
CHHHHHHHHHHHHCCCEEECCCCCEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHCCCCC
ETDTLDKMEKRVEIDLKYINNWSLWLDVKILVKTPFTLLSKDIY
CCHHHHHHHHHHEEEEEEECCEEEEEEEEEEECCCHHHHHCCCC
>Mature Secondary Structure
MDLSLRINRNTGLKGLTFWGQWALAQSFIVSLLFILAEQHTGTVEFYYRMCTILAVLASV
CCEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
PAYTFSGAYRKRDNYLTGLGRLFMGWFMTMAGLAFIAFICKADALFSRQVILEWAVYGFL
CCHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GQALLYAPLHAFSKFYQRSRKSEHKTLIVGTGELALGLAKKLSQHENLPLVGLVSNGDTP
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCC
SLEADAPRVVGAQDELLELIQTHDIRRLYITLPLSEAAKIEAMYVDLLDANVDVVWVPDL
CCCCCCCCEECCHHHHHHHHHHCCCEEEEEEEECCCCHHHHEEEEEEEECCCCEEECCCC
NSLTLLNHSVKVVDGLPAIYLNESPLTSRPTAALSKSLVEKAVALLAIILLSPVLLAVAL
CCEEEECCCEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVKLTSPGPVFFKQDRHGWNGKVIKVWKFRSMRVHDDREVKQASRNDSRITPVGRFIRRT
HHEECCCCCEEEECCCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCCCCCCHHHHHHHHC
SLDELPQLFNVLQGHMALVGPRPHAVAHNNYYSGKILAYMARHRIKPGITGLAQISGCRG
CHHHHHHHHHHHHCCCEEECCCCCEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHCCCCC
ETDTLDKMEKRVEIDLKYINNWSLWLDVKILVKTPFTLLSKDIY
CCHHHHHHHHHHEEEEEEECCEEEEEEEEEEECCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]