Click here to switch to the map view.

The map label for this gene is dusA [H]

Identifier: 77460046

GI number: 77460046

Start: 4324333

End: 4325328

Strand: Direct

Name: dusA [H]

Synonym: Pfl01_3824

Alternate gene names: 77460046

Gene position: 4324333-4325328 (Clockwise)

Preceding gene: 77460042

Following gene: 77460047

Centisome position: 67.16

GC content: 61.95

Gene sequence:

>996_bases
ATGCCCCCAATCACCGCCACACCCGCCCCACTCTCCCGCCGTTTCTCCGTCGCGCCCATGATGGATTGGACTGACCGTCA
CTGCCGGTACTTCCTACGCATCCTGTCGAAAAACGCCCTGCTCTACACCGAAATGGTCACCACCGGCGCTCTGCTCAACG
GCGATCACGAGCGTTTCCTCCGTCACAACGAAGCCGAGCACCCGTTGGCGTTGCAGTTGGGCGGTAGTGTTCCGCTGGAC
CTGGCCGCTTGTGCGCGTATGGCGCAGGAGCACGGTTACGACGAGGTGAATCTGAACGTTGGCTGCCCGAGTGATCGGGT
GCAGAACAATATGATCGGTGCGTGCCTGATGGGGCATCCGCAGTTGGTGGCGGATTGTGTGAAGGCGATGCGGGATGCGG
TGTCGATTCCGGTGACGGTGAAGCATCGGATCGGGATCAATGGGCGGGACAGTTATGAGGAGCTGTGCGATTTCGTCGGC
ACGGTGCGGGATGCCGGGTGCACCAGTTTTACGGTGCATGCGCGGATTGCGATTCTGGAGGGGCTTTCGCCGAAGGAGAA
CCGTGACATTCCGCCGTTGCGTTATGACGTGGCGGCGCGGTTGAAGGCGGATTTTCCGGAGCTGGAGTTCATTCTGAACG
GCGGGATCAAGACGATGGAGGCCTGCCATGAGCATTTGCAGACCTTTGACGGGGTGATGCTGGGGCGCGAGGCGTATCAC
AATCCTTATCTGCTGGCCGAGGTGGATCAGCAGTTGTTCGGCAGCAGCGCGCCGGTGATCAGCCGGGCCGAGGCGCTGGC
GCAGTTGCGGCCTTATATAGCCGAGCATTTGCTGGCCGGCGGCGCGATGCATCACATCACCCGGCATGTGCTGGGCCTGG
GCACCGGGTTCCCGGGGGCGCGCAAGTTTCGTCAGTTGTTGTCGGTGGATATCCACAAGGCCAAGGATCCGCTGGCGTTG
CTGGATCAGGCGGCGGAGTTGCTGGCCGGGCGTTGA

Upstream 100 bases:

>100_bases
CAAGCCCCCTCAGCACAGCATTCAGGTCCTCCCCCAAGCCCCTCCTCCTCATTCCTGATAAACTCCCCCACCTCCCAGCC
ACACCGATTCCGTACCCCCA

Downstream 100 bases:

>100_bases
TCGACGCTTGGAGCGTGTGGGCAACGAATGAGCGTTGCCCGGTGTTTTGCTGTACTGACAGGATGTCTTTTGGTTATGCC
CGCGTTCATCTGTGTTTTAT

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MPPITATPAPLSRRFSVAPMMDWTDRHCRYFLRILSKNALLYTEMVTTGALLNGDHERFLRHNEAEHPLALQLGGSVPLD
LAACARMAQEHGYDEVNLNVGCPSDRVQNNMIGACLMGHPQLVADCVKAMRDAVSIPVTVKHRIGINGRDSYEELCDFVG
TVRDAGCTSFTVHARIAILEGLSPKENRDIPPLRYDVAARLKADFPELEFILNGGIKTMEACHEHLQTFDGVMLGREAYH
NPYLLAEVDQQLFGSSAPVISRAEALAQLRPYIAEHLLAGGAMHHITRHVLGLGTGFPGARKFRQLLSVDIHKAKDPLAL
LDQAAELLAGR

Sequences:

>Translated_331_residues
MPPITATPAPLSRRFSVAPMMDWTDRHCRYFLRILSKNALLYTEMVTTGALLNGDHERFLRHNEAEHPLALQLGGSVPLD
LAACARMAQEHGYDEVNLNVGCPSDRVQNNMIGACLMGHPQLVADCVKAMRDAVSIPVTVKHRIGINGRDSYEELCDFVG
TVRDAGCTSFTVHARIAILEGLSPKENRDIPPLRYDVAARLKADFPELEFILNGGIKTMEACHEHLQTFDGVMLGREAYH
NPYLLAEVDQQLFGSSAPVISRAEALAQLRPYIAEHLLAGGAMHHITRHVLGLGTGFPGARKFRQLLSVDIHKAKDPLAL
LDQAAELLAGR
>Mature_330_residues
PPITATPAPLSRRFSVAPMMDWTDRHCRYFLRILSKNALLYTEMVTTGALLNGDHERFLRHNEAEHPLALQLGGSVPLDL
AACARMAQEHGYDEVNLNVGCPSDRVQNNMIGACLMGHPQLVADCVKAMRDAVSIPVTVKHRIGINGRDSYEELCDFVGT
VRDAGCTSFTVHARIAILEGLSPKENRDIPPLRYDVAARLKADFPELEFILNGGIKTMEACHEHLQTFDGVMLGREAYHN
PYLLAEVDQQLFGSSAPVISRAEALAQLRPYIAEHLLAGGAMHHITRHVLGLGTGFPGARKFRQLLSVDIHKAKDPLALL
DQAAELLAGR

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=233, Percent_Identity=26.6094420600858, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI31742496, Length=236, Percent_Identity=27.5423728813559, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI239788462, Length=248, Percent_Identity=26.6129032258064, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI145693211, Length=326, Percent_Identity=59.8159509202454, Blast_Score=393, Evalue=1e-111,
Organism=Escherichia coli, GI1788462, Length=222, Percent_Identity=31.5315315315315, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789660, Length=251, Percent_Identity=26.6932270916335, Blast_Score=76, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17507177, Length=168, Percent_Identity=30.952380952381, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25144369, Length=232, Percent_Identity=28.0172413793103, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323560, Length=234, Percent_Identity=26.4957264957265, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19921524, Length=237, Percent_Identity=27.0042194092827, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24580595, Length=289, Percent_Identity=26.9896193771626, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI19920448, Length=289, Percent_Identity=26.9896193771626, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 36438; Mature: 36307

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPITATPAPLSRRFSVAPMMDWTDRHCRYFLRILSKNALLYTEMVTTGALLNGDHERFL
CCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCHHHHH
RHNEAEHPLALQLGGSVPLDLAACARMAQEHGYDEVNLNVGCPSDRVQNNMIGACLMGHP
HCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCHHHHHCCCH
QLVADCVKAMRDAVSIPVTVKHRIGINGRDSYEELCDFVGTVRDAGCTSFTVHARIAILE
HHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHC
GLSPKENRDIPPLRYDVAARLKADFPELEFILNGGIKTMEACHEHLQTFDGVMLGREAYH
CCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
NPYLLAEVDQQLFGSSAPVISRAEALAQLRPYIAEHLLAGGAMHHITRHVLGLGTGFPGA
CCEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHH
RKFRQLLSVDIHKAKDPLALLDQAAELLAGR
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PPITATPAPLSRRFSVAPMMDWTDRHCRYFLRILSKNALLYTEMVTTGALLNGDHERFL
CCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCHHHHH
RHNEAEHPLALQLGGSVPLDLAACARMAQEHGYDEVNLNVGCPSDRVQNNMIGACLMGHP
HCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCHHHHHCCCH
QLVADCVKAMRDAVSIPVTVKHRIGINGRDSYEELCDFVGTVRDAGCTSFTVHARIAILE
HHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHC
GLSPKENRDIPPLRYDVAARLKADFPELEFILNGGIKTMEACHEHLQTFDGVMLGREAYH
CCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
NPYLLAEVDQQLFGSSAPVISRAEALAQLRPYIAEHLLAGGAMHHITRHVLGLGTGFPGA
CCEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHH
RKFRQLLSVDIHKAKDPLALLDQAAELLAGR
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]