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The map label for this gene is fecI [H]

Identifier: 77460008

GI number: 77460008

Start: 4284807

End: 4285307

Strand: Reverse

Name: fecI [H]

Synonym: Pfl01_3786

Alternate gene names: 77460008

Gene position: 4285307-4284807 (Counterclockwise)

Preceding gene: 77460009

Following gene: 77460007

Centisome position: 66.56

GC content: 62.67

Gene sequence:

>501_bases
ATGATCCCCATGCTGCCCCGCAGACCCGGCTTTCTCGAGCATTACGAAGAGTTGATCGGCACCTGGACCCGTCGCCTGCG
CAATCGCTCGCAGGCCGAGGATCTGGCGCACGACACCTTTGTGCGGGTGCTCGAATCCGATTCCTCGGCGGTGCAGCAAC
CTCGGGCGTATTTGCACCAGACTGCGCGCAATATCGCTGTGGACGGCTACCGGCGTGAGGACCGGCGGGGCGCCATGGAG
TCGGAGGCGATCGATCTCAGTGAGTCGCCGACCGGCGATCCGGAGCATTACATGCATGCGATCCAGCTGGCCGACTCCAT
CGAACGCGCGCTCACGGAGCTGCCGGTCAACTGCCGACGAATTTTCGTCTGGCAGAAGATCGAAGGCCTGACCCAGGCGG
AAATCGCCGAACGCCTGGGCCTGTCCAGGAACATGGTGGAAAAGTATATGATCCGCACCCTGCGGCATCTGCGTGATCGC
CTGGACGGGGCGCAATCATGA

Upstream 100 bases:

>100_bases
GGGATAGAGGAAAACAGCTTCGTCGGTGTTCCCGAGGAATCCATTGCTTCGTATTAATGGGAAGTATTACCATTCGCTCC
CCTTTCCTCAGCACGCCGCA

Downstream 100 bases:

>100_bases
GCCGTGGCGTCTTTTCATCCCCAGCCAAACAGGACATTCCATGATGGATACTCGTGATTGCGCGTGCGGGCAAACAACGG
TGCGCGATGACGCGGCACGC

Product: sigma-24 (FecI)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MIPMLPRRPGFLEHYEELIGTWTRRLRNRSQAEDLAHDTFVRVLESDSSAVQQPRAYLHQTARNIAVDGYRREDRRGAME
SEAIDLSESPTGDPEHYMHAIQLADSIERALTELPVNCRRIFVWQKIEGLTQAEIAERLGLSRNMVEKYMIRTLRHLRDR
LDGAQS

Sequences:

>Translated_166_residues
MIPMLPRRPGFLEHYEELIGTWTRRLRNRSQAEDLAHDTFVRVLESDSSAVQQPRAYLHQTARNIAVDGYRREDRRGAME
SEAIDLSESPTGDPEHYMHAIQLADSIERALTELPVNCRRIFVWQKIEGLTQAEIAERLGLSRNMVEKYMIRTLRHLRDR
LDGAQS
>Mature_166_residues
MIPMLPRRPGFLEHYEELIGTWTRRLRNRSQAEDLAHDTFVRVLESDSSAVQQPRAYLHQTARNIAVDGYRREDRRGAME
SEAIDLSESPTGDPEHYMHAIQLADSIERALTELPVNCRRIFVWQKIEGLTQAEIAERLGLSRNMVEKYMIRTLRHLRDR
LDGAQS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates the fec genes for iron dicitrate transport (Probable) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790746, Length=158, Percent_Identity=33.5443037974684, Blast_Score=77, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 19312; Mature: 19312

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPMLPRRPGFLEHYEELIGTWTRRLRNRSQAEDLAHDTFVRVLESDSSAVQQPRAYLHQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
TARNIAVDGYRREDRRGAMESEAIDLSESPTGDPEHYMHAIQLADSIERALTELPVNCRR
HHHHHHHCCCCCHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHH
IFVWQKIEGLTQAEIAERLGLSRNMVEKYMIRTLRHLRDRLDGAQS
HHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIPMLPRRPGFLEHYEELIGTWTRRLRNRSQAEDLAHDTFVRVLESDSSAVQQPRAYLHQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
TARNIAVDGYRREDRRGAMESEAIDLSESPTGDPEHYMHAIQLADSIERALTELPVNCRR
HHHHHHHCCCCCHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHH
IFVWQKIEGLTQAEIAERLGLSRNMVEKYMIRTLRHLRDRLDGAQS
HHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2254251; 7610040; 9278503; 8052622 [H]