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The map label for this gene is hemB [H]

Identifier: 77459973

GI number: 77459973

Start: 4239432

End: 4240406

Strand: Reverse

Name: hemB [H]

Synonym: Pfl01_3751

Alternate gene names: 77459973

Gene position: 4240406-4239432 (Counterclockwise)

Preceding gene: 77459974

Following gene: 77459971

Centisome position: 65.86

GC content: 63.79

Gene sequence:

>975_bases
ATGTCCAGTCAGTTCCCCGAAGCACGTCCACGCCGTCTGCGCCGCAATGCGAGCCTGCGCAGCCTGTTCCAGGAAACCGA
GTTTTCCCTCAACGATCTGGTGCTGCCGATCTTCGTCGAAGAAGAGATCGACGACTTCGTGCCGATCAAGAGCATGCCCG
GCGTGATGCGCATTCCCGAGCGCAAACTGGCCGGCGAGATCGAGCGTTACGCCCGGGCCGGGATCAAGTCGGTGATGACG
TTTGGCGTGTCTCATCACCTGGACGCCAACGGCAGCGACACCTGGCGTGAGAACGGTCTGGTGTCTCGCATGTCGCGGAT
CGCCAAGGACGCCGTGCCGGACATGATCGTGATGTCAGACACCTGCTTCTGCGAGTACACCGACCACGGCCATTGCGGCG
TGATGCACAACCATGAAGTCGACAACGACCAGACCCTGATCAACCTCGGCAAGCAAGCGGTGGCGGCGGCGCGTGCCGGT
GCCGACGTGATCGCGCCATCGGCAGCGATGGACGGCCAGGTGCGGGCGATTCGCCGGGCGCTGGACGATGCCGGCTTCTC
GCAGATTCCGATCATGGCCTACTCGACCAAATTCGCCTCGGCGCTCTACGGCCCGTTCCGCGAGGCTGGTGGCAGCGCGC
TGAAGGGCGATCGCAAAAGCTATCAGATGAACCCGATGAACCGCCGCGAAGCCCTGCGCGAATCGTTGCTCGACGAGCAG
GAAGGCGCCGACGCACTGATGGTCAAACCGGCCGGCGCGTACCTGGACATCATCCGCGACATCCGCGAAGCCTCGACCCT
GCCGCTGGCGGCGTATCAGGTCAGCGGCGAGTACGCGATGATCAAGTTCGGCGCCCAGGCCGGGGCTATCGACGAAGATC
GCGTGGTGCGCGAAAGCCTCGGTGCGATCAAACGCGCGGGTGCGGATCTGATCTTCACCTACTTTGCGATGGACCTGGCC
CTGGCCGGGATCTGA

Upstream 100 bases:

>100_bases
TGCACTGTTGATTGATCCAGGTTTGTACCCCGTCCCCCGCAAGGCATAAGCTTCGCCCCCTACGACTTTCGTCTCATCCG
CTGTTTTCAGGAAAGGCCCC

Downstream 100 bases:

>100_bases
TCCTTCAGGGGCAATTGAAAAACGGCCGGATCCCGTGATCACGGAAATACCGTTCGATCACCTTCGGATCCGCGCTGTTG
TCGGCAATCGCCACGTAGGT

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MSSQFPEARPRRLRRNASLRSLFQETEFSLNDLVLPIFVEEEIDDFVPIKSMPGVMRIPERKLAGEIERYARAGIKSVMT
FGVSHHLDANGSDTWRENGLVSRMSRIAKDAVPDMIVMSDTCFCEYTDHGHCGVMHNHEVDNDQTLINLGKQAVAAARAG
ADVIAPSAAMDGQVRAIRRALDDAGFSQIPIMAYSTKFASALYGPFREAGGSALKGDRKSYQMNPMNRREALRESLLDEQ
EGADALMVKPAGAYLDIIRDIREASTLPLAAYQVSGEYAMIKFGAQAGAIDEDRVVRESLGAIKRAGADLIFTYFAMDLA
LAGI

Sequences:

>Translated_324_residues
MSSQFPEARPRRLRRNASLRSLFQETEFSLNDLVLPIFVEEEIDDFVPIKSMPGVMRIPERKLAGEIERYARAGIKSVMT
FGVSHHLDANGSDTWRENGLVSRMSRIAKDAVPDMIVMSDTCFCEYTDHGHCGVMHNHEVDNDQTLINLGKQAVAAARAG
ADVIAPSAAMDGQVRAIRRALDDAGFSQIPIMAYSTKFASALYGPFREAGGSALKGDRKSYQMNPMNRREALRESLLDEQ
EGADALMVKPAGAYLDIIRDIREASTLPLAAYQVSGEYAMIKFGAQAGAIDEDRVVRESLGAIKRAGADLIFTYFAMDLA
LAGI
>Mature_323_residues
SSQFPEARPRRLRRNASLRSLFQETEFSLNDLVLPIFVEEEIDDFVPIKSMPGVMRIPERKLAGEIERYARAGIKSVMTF
GVSHHLDANGSDTWRENGLVSRMSRIAKDAVPDMIVMSDTCFCEYTDHGHCGVMHNHEVDNDQTLINLGKQAVAAARAGA
DVIAPSAAMDGQVRAIRRALDDAGFSQIPIMAYSTKFASALYGPFREAGGSALKGDRKSYQMNPMNRREALRESLLDEQE
GADALMVKPAGAYLDIIRDIREASTLPLAAYQVSGEYAMIKFGAQAGAIDEDRVVRESLGAIKRAGADLIFTYFAMDLAL
AGI

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=300, Percent_Identity=42, Blast_Score=227, Evalue=1e-59,
Organism=Escherichia coli, GI87081728, Length=312, Percent_Identity=76.6025641025641, Blast_Score=492, Evalue=1e-140,
Organism=Saccharomyces cerevisiae, GI6321398, Length=305, Percent_Identity=38.6885245901639, Blast_Score=210, Evalue=2e-55,
Organism=Drosophila melanogaster, GI21358291, Length=309, Percent_Identity=40.453074433657, Blast_Score=221, Evalue=4e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 35599; Mature: 35468

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSQFPEARPRRLRRNASLRSLFQETEFSLNDLVLPIFVEEEIDDFVPIKSMPGVMRIPE
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEEHHHHHHCCCCCCCCCCCCCCC
RKLAGEIERYARAGIKSVMTFGVSHHLDANGSDTWRENGLVSRMSRIAKDAVPDMIVMSD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHCCCEEEECC
TCFCEYTDHGHCGVMHNHEVDNDQTLINLGKQAVAAARAGADVIAPSAAMDGQVRAIRRA
CEEEEECCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCHHCCCCHHCCHHHHHHHHH
LDDAGFSQIPIMAYSTKFASALYGPFREAGGSALKGDRKSYQMNPMNRREALRESLLDEQ
HHHCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
EGADALMVKPAGAYLDIIRDIREASTLPLAAYQVSGEYAMIKFGAQAGAIDEDRVVRESL
CCCCEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCCCCHHHHHHHHH
GAIKRAGADLIFTYFAMDLALAGI
HHHHHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSQFPEARPRRLRRNASLRSLFQETEFSLNDLVLPIFVEEEIDDFVPIKSMPGVMRIPE
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEEHHHHHHCCCCCCCCCCCCCCC
RKLAGEIERYARAGIKSVMTFGVSHHLDANGSDTWRENGLVSRMSRIAKDAVPDMIVMSD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHCCCEEEECC
TCFCEYTDHGHCGVMHNHEVDNDQTLINLGKQAVAAARAGADVIAPSAAMDGQVRAIRRA
CEEEEECCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCHHCCCCHHCCHHHHHHHHH
LDDAGFSQIPIMAYSTKFASALYGPFREAGGSALKGDRKSYQMNPMNRREALRESLLDEQ
HHHCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
EGADALMVKPAGAYLDIIRDIREASTLPLAAYQVSGEYAMIKFGAQAGAIDEDRVVRESL
CCCCEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCCCCHHHHHHHHH
GAIKRAGADLIFTYFAMDLALAGI
HHHHHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2656410; 2464127; 9278503; 8439296; 10194344; 11444968; 11909869 [H]