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The map label for this gene is polB [H]

Identifier: 77459956

GI number: 77459956

Start: 4218772

End: 4221135

Strand: Reverse

Name: polB [H]

Synonym: Pfl01_3734

Alternate gene names: 77459956

Gene position: 4221135-4218772 (Counterclockwise)

Preceding gene: 77459958

Following gene: 77459952

Centisome position: 65.56

GC content: 63.16

Gene sequence:

>2364_bases
GTGGTGGATTTACAGCAGGGCTTCGTCCTGACCCGGCATTGGCGCGACACCCCGGCCGGCACCGAAGTCGAGTTCTGGCT
GGCGACCGACGCCGGGCCGCGCCGTGTGCGCCTGCCGCATCAGCCGTCGGTGGCATTCATCCCTGCGGCGCAGCGCGAGC
AGGCCGAGCGGGTGCTGGCGGACGAAAAGAACGTCGAACTGCGTCCCCTGGCTTTGCAGGATTTCGAGCATCGCCCGGTG
CTCGGTCTGTACTGCCAGCAACACGGTCAGTTGATGCGCCTCGAAACCGCGCTCAACCGCCACAGTGTCGATGTCTTCGA
AGCCGATGTGCGACCACCCGAGCGCTACCTGATGGAGCGTTTCATCACCGCGCCTGTCTGGTTCAGCGGCACGCCCGATG
CCGATGGCGTGTTGCTCGACGCCCACCTCAAGCCTGCCCCCGACTATCGTCCGCAACTGCGGCTGGTCTCGCTGGACATC
GAAACCACCGAGCAGGGCGAGTTGTATTCCATCGCGCTGGAGGGCTGCGGCGAGCGGCAGGTGTACATGCTCGGCGCACC
GAATGGCGATGCCAGCATCGTCGATTTCGATCTCGAGTACTGCGAATCGCGCACCTTGATCCTCAAGAAGCTCAACGACT
GGTTCGCCCGTCACGACCCCGACGCGATCATCGGCTGGAACCTCGTGCAGTTCGATCTGCGCATCCTCCACGAACACGCC
CGACGCCTCGGCGTGCCGCTCAAGCTCGGGCGAGGCGGCGAGGAAATGCAGTGGCGCGAACACGGCAGCCGCAATCACTA
CTTCGCTGCAGCGGCGGGACGGTTGATCATCGACGGCATCGAATCCCTGCGTTCGGCGACCTGGAGCTTTCCGTCGTTCA
GCCTGGAAAACGTCGCCCAGACCCTGCTCGGCGAGGGCAAGTCGATCGACAACCCGTACCAGCGCATGGACGAGATCAAC
CGCATGTTCGCCGAGGACAAACCGGCGCTGGCCAAGTACAACCTCAAGGACTGCGAACTGGTCACGCGGATCTTCGCCAA
GACCGAGCTGCTGAAATTTTTACTGGAGCGCGCCAGCGTCACCGGCCTGCCGGCGGATCGCAGCGGCGGCTCGGTGGCGG
CATTCACGCATTTGTATATGCCGCTGATGCACCGCCAGGGTTTTGTGGCGCCGAACCTTGGCACCAATCCGCCGCAGGCC
AGCCCCGGCGGGTTTGTCATGGACTCGCGACCGGGGCTTTACGAATCGGTGCTGGTGCTCGACTACAAGAGTCTTTACCC
GTCGATCATTCGTACCTTTCTGATTGATCCGGTGGGGTTGATCGAAGGCTTGCAGCATCCGGATGACAGCGACTCGGTGC
CGGGCTTTCGCGGTGCGCGTTTTTCCCGAACCCGGCATTGCCTGCCGTCCATCGTCGCCCGGGTCGCCGAGGGCCGCGAG
ACCGCCAAGCGCGAGCACAACGCGCCGCTGTCCCAGGCGCTGAAGATCATCATGAACGCCTTCTACGGCGTGCTCGGTTC
CAGTGGTTGCCGGTTTTTCGATACGCGACTGGCGTCTTCGATCACCCTGCGCGGCCACGAAATCATGCTGCGCACTCGAC
AGCTGATCGAGGCTCAGGGGCATGCGGTGATTTATGGCGATACCGACTCGACCTTCGTCTGGCTGCGTCGCCCCCACGGT
CAGGAAGAAGCGGCGCAAATCGGTCGGGCCTTGGTGCAGCACGTCAACGATTGGTGGCGCGAGCATGTGCGTGAGCAATA
CGGTCTGGAAAGCGCCCTCGAATTGCAGTTCGAAACTCACTACAAGCGCTTTTTGATGCCGACGATTCGCGGCGCCGAGG
AGGGCAGCAAAAAGCGCTACGCCGGCCTGGTGACCCGCGCCGACGGCAGCGAAGAAATGGTCTACAAAGGCCTGGAAACC
GTGCGCACCGACTGGTCGCTGCTGGCCCGGCAATTTCAGCAGGAGTTGTACGAGCGGATCTTCCAGCGCAAGCCGTATCA
GGATTACGTGCGCGAGTATGTGCGCAAGACCCTGGCCGGCGAGTTCGACGAGCGACTGGTCTATCGCAAGCGCCTGCGCC
GGACTCTCGATGACTACGAACGCAACGTGCCGCCTCATGTTCGTGCCGCACGGTTGGCCGACGATTACAACGCGCAACAC
GGTCGCCCGCGGCAATACCAGAACGGTGGCTGGATCAGCTACGTCATCACCCTGGCCGGGCCCGAGCCGCTGGAAGTGCG
TCGCGCCGCCATCGACTACGACCACTACATCACCCGCCAGCTGCAACCGGTGGCGGATGCGATCCTGCCGTTTGTCGACG
ACGATTTTTCAACCCTGATCGGGGGGCAACTGGGCCTGTTTTGA

Upstream 100 bases:

>100_bases
AAAAAACGTCATTTGACCGGGTTTTTCATGGCGAGGCCTTTGTCACGGGTTACGGCAATGCCGCGTAGAATGCCCGTCTG
AATCAATTCCTGAGGTTGCA

Downstream 100 bases:

>100_bases
GTCCAGCAGTTGCAGGGTCCATTCGTCCTGAATACCGTGGAAATAACGCTCCAGCGCCTGATGCCAGAGGCTGTCGTCGT
CAGCGAACTGGGCGAACAGG

Product: DNA polymerase II

Products: NA

Alternate protein names: Pol II [H]

Number of amino acids: Translated: 787; Mature: 787

Protein sequence:

>787_residues
MVDLQQGFVLTRHWRDTPAGTEVEFWLATDAGPRRVRLPHQPSVAFIPAAQREQAERVLADEKNVELRPLALQDFEHRPV
LGLYCQQHGQLMRLETALNRHSVDVFEADVRPPERYLMERFITAPVWFSGTPDADGVLLDAHLKPAPDYRPQLRLVSLDI
ETTEQGELYSIALEGCGERQVYMLGAPNGDASIVDFDLEYCESRTLILKKLNDWFARHDPDAIIGWNLVQFDLRILHEHA
RRLGVPLKLGRGGEEMQWREHGSRNHYFAAAAGRLIIDGIESLRSATWSFPSFSLENVAQTLLGEGKSIDNPYQRMDEIN
RMFAEDKPALAKYNLKDCELVTRIFAKTELLKFLLERASVTGLPADRSGGSVAAFTHLYMPLMHRQGFVAPNLGTNPPQA
SPGGFVMDSRPGLYESVLVLDYKSLYPSIIRTFLIDPVGLIEGLQHPDDSDSVPGFRGARFSRTRHCLPSIVARVAEGRE
TAKREHNAPLSQALKIIMNAFYGVLGSSGCRFFDTRLASSITLRGHEIMLRTRQLIEAQGHAVIYGDTDSTFVWLRRPHG
QEEAAQIGRALVQHVNDWWREHVREQYGLESALELQFETHYKRFLMPTIRGAEEGSKKRYAGLVTRADGSEEMVYKGLET
VRTDWSLLARQFQQELYERIFQRKPYQDYVREYVRKTLAGEFDERLVYRKRLRRTLDDYERNVPPHVRAARLADDYNAQH
GRPRQYQNGGWISYVITLAGPEPLEVRRAAIDYDHYITRQLQPVADAILPFVDDDFSTLIGGQLGLF

Sequences:

>Translated_787_residues
MVDLQQGFVLTRHWRDTPAGTEVEFWLATDAGPRRVRLPHQPSVAFIPAAQREQAERVLADEKNVELRPLALQDFEHRPV
LGLYCQQHGQLMRLETALNRHSVDVFEADVRPPERYLMERFITAPVWFSGTPDADGVLLDAHLKPAPDYRPQLRLVSLDI
ETTEQGELYSIALEGCGERQVYMLGAPNGDASIVDFDLEYCESRTLILKKLNDWFARHDPDAIIGWNLVQFDLRILHEHA
RRLGVPLKLGRGGEEMQWREHGSRNHYFAAAAGRLIIDGIESLRSATWSFPSFSLENVAQTLLGEGKSIDNPYQRMDEIN
RMFAEDKPALAKYNLKDCELVTRIFAKTELLKFLLERASVTGLPADRSGGSVAAFTHLYMPLMHRQGFVAPNLGTNPPQA
SPGGFVMDSRPGLYESVLVLDYKSLYPSIIRTFLIDPVGLIEGLQHPDDSDSVPGFRGARFSRTRHCLPSIVARVAEGRE
TAKREHNAPLSQALKIIMNAFYGVLGSSGCRFFDTRLASSITLRGHEIMLRTRQLIEAQGHAVIYGDTDSTFVWLRRPHG
QEEAAQIGRALVQHVNDWWREHVREQYGLESALELQFETHYKRFLMPTIRGAEEGSKKRYAGLVTRADGSEEMVYKGLET
VRTDWSLLARQFQQELYERIFQRKPYQDYVREYVRKTLAGEFDERLVYRKRLRRTLDDYERNVPPHVRAARLADDYNAQH
GRPRQYQNGGWISYVITLAGPEPLEVRRAAIDYDHYITRQLQPVADAILPFVDDDFSTLIGGQLGLF
>Mature_787_residues
MVDLQQGFVLTRHWRDTPAGTEVEFWLATDAGPRRVRLPHQPSVAFIPAAQREQAERVLADEKNVELRPLALQDFEHRPV
LGLYCQQHGQLMRLETALNRHSVDVFEADVRPPERYLMERFITAPVWFSGTPDADGVLLDAHLKPAPDYRPQLRLVSLDI
ETTEQGELYSIALEGCGERQVYMLGAPNGDASIVDFDLEYCESRTLILKKLNDWFARHDPDAIIGWNLVQFDLRILHEHA
RRLGVPLKLGRGGEEMQWREHGSRNHYFAAAAGRLIIDGIESLRSATWSFPSFSLENVAQTLLGEGKSIDNPYQRMDEIN
RMFAEDKPALAKYNLKDCELVTRIFAKTELLKFLLERASVTGLPADRSGGSVAAFTHLYMPLMHRQGFVAPNLGTNPPQA
SPGGFVMDSRPGLYESVLVLDYKSLYPSIIRTFLIDPVGLIEGLQHPDDSDSVPGFRGARFSRTRHCLPSIVARVAEGRE
TAKREHNAPLSQALKIIMNAFYGVLGSSGCRFFDTRLASSITLRGHEIMLRTRQLIEAQGHAVIYGDTDSTFVWLRRPHG
QEEAAQIGRALVQHVNDWWREHVREQYGLESALELQFETHYKRFLMPTIRGAEEGSKKRYAGLVTRADGSEEMVYKGLET
VRTDWSLLARQFQQELYERIFQRKPYQDYVREYVRKTLAGEFDERLVYRKRLRRTLDDYERNVPPHVRAARLADDYNAQH
GRPRQYQNGGWISYVITLAGPEPLEVRRAAIDYDHYITRQLQPVADAILPFVDDDFSTLIGGQLGLF

Specific function: Thought to be involved in DNA repair and/or mutagenesis [H]

COG id: COG0417

COG function: function code L; DNA polymerase elongation subunit (family B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-B family [H]

Homologues:

Organism=Homo sapiens, GI106507301, Length=657, Percent_Identity=25.1141552511416, Blast_Score=136, Evalue=1e-31,
Organism=Homo sapiens, GI156616275, Length=606, Percent_Identity=26.8976897689769, Blast_Score=134, Evalue=3e-31,
Organism=Escherichia coli, GI1786246, Length=784, Percent_Identity=68.3673469387755, Blast_Score=1097, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17559488, Length=699, Percent_Identity=25.6080114449213, Blast_Score=149, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI32565317, Length=649, Percent_Identity=24.8073959938367, Blast_Score=128, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6320101, Length=628, Percent_Identity=26.4331210191083, Blast_Score=131, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6324227, Length=507, Percent_Identity=26.4299802761341, Blast_Score=121, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24648780, Length=677, Percent_Identity=23.9290989660266, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24664937, Length=785, Percent_Identity=24.8407643312102, Blast_Score=118, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006172
- InterPro:   IPR017964
- InterPro:   IPR006133
- InterPro:   IPR006134
- InterPro:   IPR023211
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00136 DNA_pol_B; PF03104 DNA_pol_B_exo [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 89824; Mature: 89824

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00116 DNA_POLYMERASE_B

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDLQQGFVLTRHWRDTPAGTEVEFWLATDAGPRRVRLPHQPSVAFIPAAQREQAERVLA
CCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCEEECCCCCCEEEECCHHHHHHHHHHC
DEKNVELRPLALQDFEHRPVLGLYCQQHGQLMRLETALNRHSVDVFEADVRPPERYLMER
CCCCCEEEEEECCCCCCCCHHHHHHHHCCCEEHHHHHHCCCCCEEEECCCCCHHHHHHHH
FITAPVWFSGTPDADGVLLDAHLKPAPDYRPQLRLVSLDIETTEQGELYSIALEGCGERQ
HHHCCEEECCCCCCCCEEEECCCCCCCCCCCCEEEEEEEECCCCCCCEEEEEECCCCCEE
VYMLGAPNGDASIVDFDLEYCESRTLILKKLNDWFARHDPDAIIGWNLVQFDLRILHEHA
EEEEECCCCCCEEEEEEHHHHCCHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHH
RRLGVPLKLGRGGEEMQWREHGSRNHYFAAAAGRLIIDGIESLRSATWSFPSFSLENVAQ
HHCCCCEEECCCCCCCHHHHCCCCCCEEEHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
TLLGEGKSIDNPYQRMDEINRMFAEDKPALAKYNLKDCELVTRIFAKTELLKFLLERASV
HHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
TGLPADRSGGSVAAFTHLYMPLMHRQGFVAPNLGTNPPQASPGGFVMDSRPGLYESVLVL
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHH
DYKSLYPSIIRTFLIDPVGLIEGLQHPDDSDSVPGFRGARFSRTRHCLPSIVARVAEGRE
HHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TAKREHNAPLSQALKIIMNAFYGVLGSSGCRFFDTRLASSITLRGHEIMLRTRQLIEAQG
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEECCHHHHHHHHHHHHCCC
HAVIYGDTDSTFVWLRRPHGQEEAAQIGRALVQHVNDWWREHVREQYGLESALELQFETH
CEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
YKRFLMPTIRGAEEGSKKRYAGLVTRADGSEEMVYKGLETVRTDWSLLARQFQQELYERI
HHHHHHHHHCCCCCCCCHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQRKPYQDYVREYVRKTLAGEFDERLVYRKRLRRTLDDYERNVPPHVRAARLADDYNAQH
HCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCC
GRPRQYQNGGWISYVITLAGPEPLEVRRAAIDYDHYITRQLQPVADAILPFVDDDFSTLI
CCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
GGQLGLF
CCCCCCC
>Mature Secondary Structure
MVDLQQGFVLTRHWRDTPAGTEVEFWLATDAGPRRVRLPHQPSVAFIPAAQREQAERVLA
CCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCEEECCCCCCEEEECCHHHHHHHHHHC
DEKNVELRPLALQDFEHRPVLGLYCQQHGQLMRLETALNRHSVDVFEADVRPPERYLMER
CCCCCEEEEEECCCCCCCCHHHHHHHHCCCEEHHHHHHCCCCCEEEECCCCCHHHHHHHH
FITAPVWFSGTPDADGVLLDAHLKPAPDYRPQLRLVSLDIETTEQGELYSIALEGCGERQ
HHHCCEEECCCCCCCCEEEECCCCCCCCCCCCEEEEEEEECCCCCCCEEEEEECCCCCEE
VYMLGAPNGDASIVDFDLEYCESRTLILKKLNDWFARHDPDAIIGWNLVQFDLRILHEHA
EEEEECCCCCCEEEEEEHHHHCCHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHH
RRLGVPLKLGRGGEEMQWREHGSRNHYFAAAAGRLIIDGIESLRSATWSFPSFSLENVAQ
HHCCCCEEECCCCCCCHHHHCCCCCCEEEHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
TLLGEGKSIDNPYQRMDEINRMFAEDKPALAKYNLKDCELVTRIFAKTELLKFLLERASV
HHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
TGLPADRSGGSVAAFTHLYMPLMHRQGFVAPNLGTNPPQASPGGFVMDSRPGLYESVLVL
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHH
DYKSLYPSIIRTFLIDPVGLIEGLQHPDDSDSVPGFRGARFSRTRHCLPSIVARVAEGRE
HHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TAKREHNAPLSQALKIIMNAFYGVLGSSGCRFFDTRLASSITLRGHEIMLRTRQLIEAQG
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEECCHHHHHHHHHHHHCCC
HAVIYGDTDSTFVWLRRPHGQEEAAQIGRALVQHVNDWWREHVREQYGLESALELQFETH
CEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
YKRFLMPTIRGAEEGSKKRYAGLVTRADGSEEMVYKGLETVRTDWSLLARQFQQELYERI
HHHHHHHHHCCCCCCCCHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQRKPYQDYVREYVRKTLAGEFDERLVYRKRLRRTLDDYERNVPPHVRAARLADDYNAQH
HCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCC
GRPRQYQNGGWISYVITLAGPEPLEVRRAAIDYDHYITRQLQPVADAILPFVDDDFSTLI
CCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
GGQLGLF
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2034216; 2261080; 1630901; 9278503; 2217198; 9079692 [H]