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The map label for this gene is cysG [H]

Identifier: 77459803

GI number: 77459803

Start: 4046399

End: 4047793

Strand: Reverse

Name: cysG [H]

Synonym: Pfl01_3581

Alternate gene names: 77459803

Gene position: 4047793-4046399 (Counterclockwise)

Preceding gene: 77459804

Following gene: 77459794

Centisome position: 62.87

GC content: 63.8

Gene sequence:

>1395_bases
ATGAAATATCTGCCGCTGTTTCACAACCTGCGCGGCAGTCGTGTGTTGGTTGTTGGTGGGGGGGAGATTGCCTTGCGCAA
GTCCCGCCTGCTGGCCGATGCCGGTGCGCTGCTGCGGGTGGTCGCACCTGAAATCGAAGCGCAACTGCGCGAACTGGTTG
CCGCCAGCGGTGGCGAATGCCTGCTGCGTGGCTACGCCGAAGCGGATCTGGACGGTTGTGGGCTGATCATTGCCGCCACC
GACGACGAAGCGTTGAACGCGCACGTCTCCGCCGATGCCCACCGGCGCTGCGTGCCGGTCAATGTGGTCGATGCGCCGGC
GCTGTGCAGCGTGATCTTCCCGGCAATCGTCGATCGTTCACCGCTGATCATCGCGGTCTCCAGCGGCGGCGATGCGCCGG
TGCTGGCCCGGCTGATCCGCGCCAAGATCGAAACCTGGATTCCTTCGACCTACGGCCATCTGGCCGGTCTGGCCGCGCGG
TTTCGTGATCAGGTCAAAGGCCTGTTTCCTGATGTGCAGCAGCGTCGCGGTTTTTGGGAAGACGTGTTTCAGGGGCCGAT
TGCCGACCGGCAGCTGGCAGGACAGGGCGCCGAAGCCGAACGTTTGCTGCAAGCCAAGATTGACGGCGAAGCCATGGTCA
CGACCGGTGAGGTGTATCTGGTTGGGGCAGGACCTGGTGATCCGGATCTGCTGACCTTCCGCGCCTTGCGTCTGATGCAG
CAAGCCGACGTGGTGCTGTACGACCGCTTGGTGGCTCCGGCGATTCTTGAGCTGTGCCGTCGCGATGCCGAGCGGGTCTA
CGTCGGCAAGCGTCGCGCCGATCACGCCGTGCCGCAGGATCAAATCAACCAGCAACTGGTGGATCTGGCCAAGGCCGGCA
AGCGTGTGGTGCGGTTGAAGGGCGGGGATCCGTTCATCTTCGGCCGTGGCGGCGAAGAGATCGAGGAACTGGCGGCCCAT
GGCATCCCGTTTCAAGTGGTGCCGGGCATCACGGCGGCCAGCGGTTGCGCGGCGTATGCCGGGATTCCGCTGACTCATCG
CGACTACGCTCAGTCGGTGCGTTTCGTCACCGGGCACTTGAAGGACGGTTCGACCGATCTGCCGTGGTCGGATCTCGTCG
CTCCGGCTCAGACGCTGGTGTTCTACATGGGGTTGGTGGGGCTGCCGATCATTTGCGAGCAGTTGATCAAACATGGTCGT
GCGGCCGAAACGCCGGCCGCGCTGATCCAGCAGGGCACCACGGTCAATCAACGGGTCTTCACCGGCACGCTGGCTGACCT
GCCACGTCTGGTGGCGGAGCATGAAGTGCATGCGCCGACGCTGGTGATCGTCGGCGAAGTGGTGCAACTGCGCGAGAAAC
TTGCGTGGTTCGAGGGCGCTCAGGGTCAAATCTGA

Upstream 100 bases:

>100_bases
CCTGGTGGCCGTGCTGGAAAACTACCAGCAGGCCGACGGTTCGATCCGTGTGCCGGACGTGCTGAAGCCGTACATGGGTG
GCCTTGAGGTCATCGGTTAA

Downstream 100 bases:

>100_bases
CGACTCTGAAGGGATTGTTCCCACGCTGTGGGAACAATCCTGCTTTCAACCTCTGCGCCAGACTCCTTTCCCGCCCAACC
GGTCCCGGTCATGGGCAACA

Product: precorrin-2 dehydrogenase / uroporphyrinogen-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MKYLPLFHNLRGSRVLVVGGGEIALRKSRLLADAGALLRVVAPEIEAQLRELVAASGGECLLRGYAEADLDGCGLIIAAT
DDEALNAHVSADAHRRCVPVNVVDAPALCSVIFPAIVDRSPLIIAVSSGGDAPVLARLIRAKIETWIPSTYGHLAGLAAR
FRDQVKGLFPDVQQRRGFWEDVFQGPIADRQLAGQGAEAERLLQAKIDGEAMVTTGEVYLVGAGPGDPDLLTFRALRLMQ
QADVVLYDRLVAPAILELCRRDAERVYVGKRRADHAVPQDQINQQLVDLAKAGKRVVRLKGGDPFIFGRGGEEIEELAAH
GIPFQVVPGITAASGCAAYAGIPLTHRDYAQSVRFVTGHLKDGSTDLPWSDLVAPAQTLVFYMGLVGLPIICEQLIKHGR
AAETPAALIQQGTTVNQRVFTGTLADLPRLVAEHEVHAPTLVIVGEVVQLREKLAWFEGAQGQI

Sequences:

>Translated_464_residues
MKYLPLFHNLRGSRVLVVGGGEIALRKSRLLADAGALLRVVAPEIEAQLRELVAASGGECLLRGYAEADLDGCGLIIAAT
DDEALNAHVSADAHRRCVPVNVVDAPALCSVIFPAIVDRSPLIIAVSSGGDAPVLARLIRAKIETWIPSTYGHLAGLAAR
FRDQVKGLFPDVQQRRGFWEDVFQGPIADRQLAGQGAEAERLLQAKIDGEAMVTTGEVYLVGAGPGDPDLLTFRALRLMQ
QADVVLYDRLVAPAILELCRRDAERVYVGKRRADHAVPQDQINQQLVDLAKAGKRVVRLKGGDPFIFGRGGEEIEELAAH
GIPFQVVPGITAASGCAAYAGIPLTHRDYAQSVRFVTGHLKDGSTDLPWSDLVAPAQTLVFYMGLVGLPIICEQLIKHGR
AAETPAALIQQGTTVNQRVFTGTLADLPRLVAEHEVHAPTLVIVGEVVQLREKLAWFEGAQGQI
>Mature_464_residues
MKYLPLFHNLRGSRVLVVGGGEIALRKSRLLADAGALLRVVAPEIEAQLRELVAASGGECLLRGYAEADLDGCGLIIAAT
DDEALNAHVSADAHRRCVPVNVVDAPALCSVIFPAIVDRSPLIIAVSSGGDAPVLARLIRAKIETWIPSTYGHLAGLAAR
FRDQVKGLFPDVQQRRGFWEDVFQGPIADRQLAGQGAEAERLLQAKIDGEAMVTTGEVYLVGAGPGDPDLLTFRALRLMQ
QADVVLYDRLVAPAILELCRRDAERVYVGKRRADHAVPQDQINQQLVDLAKAGKRVVRLKGGDPFIFGRGGEEIEELAAH
GIPFQVVPGITAASGCAAYAGIPLTHRDYAQSVRFVTGHLKDGSTDLPWSDLVAPAQTLVFYMGLVGLPIICEQLIKHGR
AAETPAALIQQGTTVNQRVFTGTLADLPRLVAEHEVHAPTLVIVGEVVQLREKLAWFEGAQGQI

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=460, Percent_Identity=53.4782608695652, Blast_Score=452, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6322922, Length=234, Percent_Identity=36.3247863247863, Blast_Score=139, Evalue=9e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 49876; Mature: 49876

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYLPLFHNLRGSRVLVVGGGEIALRKSRLLADAGALLRVVAPEIEAQLRELVAASGGEC
CCCCCCHHCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LLRGYAEADLDGCGLIIAATDDEALNAHVSADAHRRCVPVNVVDAPALCSVIFPAIVDRS
EEECCCCCCCCCCEEEEEECCCHHHEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHCCC
PLIIAVSSGGDAPVLARLIRAKIETWIPSTYGHLAGLAARFRDQVKGLFPDVQQRRGFWE
CEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHH
DVFQGPIADRQLAGQGAEAERLLQAKIDGEAMVTTGEVYLVGAGPGDPDLLTFRALRLMQ
HHHCCCCCCHHHCCCCCHHHHHHHHHCCCCEEEEECCEEEEECCCCCCHHHHHHHHHHHH
QADVVLYDRLVAPAILELCRRDAERVYVGKRRADHAVPQDQINQQLVDLAKAGKRVVRLK
HCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHCCCEEEEEE
GGDPFIFGRGGEEIEELAAHGIPFQVVPGITAASGCAAYAGIPLTHRDYAQSVRFVTGHL
CCCEEEECCCCHHHHHHHHCCCCEEECCCCCHHCCCHHHCCCCCCHHHHHHHHHHHEEEC
KDGSTDLPWSDLVAPAQTLVFYMGLVGLPIICEQLIKHGRAAETPAALIQQGTTVNQRVF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHH
TGTLADLPRLVAEHEVHAPTLVIVGEVVQLREKLAWFEGAQGQI
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKYLPLFHNLRGSRVLVVGGGEIALRKSRLLADAGALLRVVAPEIEAQLRELVAASGGEC
CCCCCCHHCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LLRGYAEADLDGCGLIIAATDDEALNAHVSADAHRRCVPVNVVDAPALCSVIFPAIVDRS
EEECCCCCCCCCCEEEEEECCCHHHEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHCCC
PLIIAVSSGGDAPVLARLIRAKIETWIPSTYGHLAGLAARFRDQVKGLFPDVQQRRGFWE
CEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHH
DVFQGPIADRQLAGQGAEAERLLQAKIDGEAMVTTGEVYLVGAGPGDPDLLTFRALRLMQ
HHHCCCCCCHHHCCCCCHHHHHHHHHCCCCEEEEECCEEEEECCCCCCHHHHHHHHHHHH
QADVVLYDRLVAPAILELCRRDAERVYVGKRRADHAVPQDQINQQLVDLAKAGKRVVRLK
HCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHCCCEEEEEE
GGDPFIFGRGGEEIEELAAHGIPFQVVPGITAASGCAAYAGIPLTHRDYAQSVRFVTGHL
CCCEEEECCCCHHHHHHHHCCCCEEECCCCCHHCCCHHHCCCCCCHHHHHHHHHHHEEEC
KDGSTDLPWSDLVAPAQTLVFYMGLVGLPIICEQLIKHGRAAETPAALIQQGTTVNQRVF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHH
TGTLADLPRLVAEHEVHAPTLVIVGEVVQLREKLAWFEGAQGQI
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA