BacMap

Definition	Pseudomonas fluorescens Pf0-1 chromosome, complete genome.
Accession	NC_007492
Length	6,438,405

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The map label for this gene is yvaG [H]

Identifier: 77459744

GI number: 77459744

Start: 4001240

End: 4002037

Strand: Reverse

Name: yvaG [H]

Synonym: Pfl01_3522

Alternate gene names: 77459744

Gene position: 4002037-4001240 (Counterclockwise)

Preceding gene: 77459745

Following gene: 77459743

Centisome position: 62.16

GC content: 65.29

Gene sequence:

>798_bases
ATGAAGATCGATCTGAGCGGAAAACTGGCGATTGTCAGCGGCAGCACGGCGGGCATCGGTTTGGGCATCAGCCAGTCGCT
GGCCGAGGCGGGCGCCACGGTGGTGGTGATCGGTCGCGACGCGGCCAAGGTCGAACAGGCGCTTACAAGCATTCGAGACA
AGGTGCCGGGCGCGCAACTGCGCGGTCTGACGGCGGATCTGGGCACGGCCGAAGGCGCGCAGAAACTGTTCGCCGCCGAA
CCGAAAGCCGACATCCTGGTGAACAACCTCGGCATCTACAACGCCGTGGATTTCTTCGACGCGCCGGACGATGAGTGGAC
GCGCTTCTATGAGATCAACGTGATCTCCGGCGTGCGCTTGTCGCGGCATTACGTGCCGGCGATGGTCGAGCAGGGCTGGG
GCCGGGTGATCTTCCTGTCTTCGGAATCCGGCGTAGCCACCCCGGCCGACATGATCAACTACGGCGTGACCAAAAGCGCC
AACCTCGCGGTGTCCCATGGCCTGGCCAAACGCCTGGCGGGCACCGGGGTTACGGTCAACGCGATCCTGCCGGGGCCGAC
CTTCACCGACGGTGTCGAGGAAATGCTCAAGGATGCCGCCGCCGAGTCTGGCCGCAGCCTGCGGGACGAGGCTGACGCGT
TTGTCCGCCGGGCCCGCCCGAGCTCGATCATCCAGCGCGTGGCGGATGTCGAAGAGGTCGCGCACCTGGTCACCTACATC
GCTTCACCGCTGTCTTCAGCTACCACTGGCGCCGCGTTGCGGGTCGACGGTGGCGTGGTCGACAGCCTGACTATCTGA

Upstream 100 bases:

>100_bases
CGGCGCTGTCGGCGGTCATTCCGGCCGGTTTCTGGCAGGCCATGCGTGAAGCGAAACTGGTGTCCGAGCGTGCACCATTA
CCCATCGACGAGGTGAAAGC

Downstream 100 bases:

>100_bases
ATTTATCTAGAGAGACTTTTTATTTATGGCAACTGCATCAGCAACCATCGACATTCCGGCTTCGGCCGAACAGGTCTGGC
AATTGATTGGCGGCTTCAAC

Product: Short-chain dehydrogenase/reductase SDR

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MKIDLSGKLAIVSGSTAGIGLGISQSLAEAGATVVVIGRDAAKVEQALTSIRDKVPGAQLRGLTADLGTAEGAQKLFAAE
PKADILVNNLGIYNAVDFFDAPDDEWTRFYEINVISGVRLSRHYVPAMVEQGWGRVIFLSSESGVATPADMINYGVTKSA
NLAVSHGLAKRLAGTGVTVNAILPGPTFTDGVEEMLKDAAAESGRSLRDEADAFVRRARPSSIIQRVADVEEVAHLVTYI
ASPLSSATTGAALRVDGGVVDSLTI

Sequences:

>Translated_265_residues
MKIDLSGKLAIVSGSTAGIGLGISQSLAEAGATVVVIGRDAAKVEQALTSIRDKVPGAQLRGLTADLGTAEGAQKLFAAE
PKADILVNNLGIYNAVDFFDAPDDEWTRFYEINVISGVRLSRHYVPAMVEQGWGRVIFLSSESGVATPADMINYGVTKSA
NLAVSHGLAKRLAGTGVTVNAILPGPTFTDGVEEMLKDAAAESGRSLRDEADAFVRRARPSSIIQRVADVEEVAHLVTYI
ASPLSSATTGAALRVDGGVVDSLTI
>Mature_265_residues
MKIDLSGKLAIVSGSTAGIGLGISQSLAEAGATVVVIGRDAAKVEQALTSIRDKVPGAQLRGLTADLGTAEGAQKLFAAE
PKADILVNNLGIYNAVDFFDAPDDEWTRFYEINVISGVRLSRHYVPAMVEQGWGRVIFLSSESGVATPADMINYGVTKSA
NLAVSHGLAKRLAGTGVTVNAILPGPTFTDGVEEMLKDAAAESGRSLRDEADAFVRRARPSSIIQRVADVEEVAHLVTYI
ASPLSSATTGAALRVDGGVVDSLTI

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=267, Percent_Identity=29.5880149812734, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI5031737, Length=258, Percent_Identity=27.1317829457364, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI126723191, Length=186, Percent_Identity=31.7204301075269, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI66933014, Length=257, Percent_Identity=27.6264591439689, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI126723750, Length=269, Percent_Identity=27.5092936802974, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI7705855, Length=184, Percent_Identity=30.9782608695652, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI33667109, Length=260, Percent_Identity=28.4615384615385, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI7705925, Length=259, Percent_Identity=25.4826254826255, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI7706523, Length=205, Percent_Identity=26.8292682926829, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI7706318, Length=257, Percent_Identity=26.8482490272374, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI225579078, Length=206, Percent_Identity=28.6407766990291, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787335, Length=261, Percent_Identity=31.4176245210728, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI87082100, Length=262, Percent_Identity=30.1526717557252, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1787905, Length=267, Percent_Identity=29.2134831460674, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786812, Length=261, Percent_Identity=29.5019157088123, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082160, Length=269, Percent_Identity=27.8810408921933, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789208, Length=263, Percent_Identity=28.1368821292776, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788459, Length=259, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790717, Length=262, Percent_Identity=24.8091603053435, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI2367365, Length=257, Percent_Identity=28.4046692607004, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1789057, Length=266, Percent_Identity=24.4360902255639, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787820, Length=182, Percent_Identity=29.1208791208791, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI2367175, Length=260, Percent_Identity=26.1538461538462, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789378, Length=264, Percent_Identity=27.6515151515151, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17561402, Length=261, Percent_Identity=31.0344827586207, Blast_Score=111, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17560676, Length=261, Percent_Identity=29.8850574712644, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25147288, Length=256, Percent_Identity=29.296875, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71994604, Length=258, Percent_Identity=29.8449612403101, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71994600, Length=258, Percent_Identity=28.2945736434109, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI193204405, Length=268, Percent_Identity=28.7313432835821, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17531453, Length=273, Percent_Identity=29.6703296703297, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17555706, Length=260, Percent_Identity=27.6923076923077, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17568967, Length=196, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17562906, Length=265, Percent_Identity=27.5471698113208, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17559552, Length=233, Percent_Identity=28.755364806867, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17557668, Length=199, Percent_Identity=29.6482412060301, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17562908, Length=267, Percent_Identity=28.8389513108614, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI115534694, Length=260, Percent_Identity=27.3076923076923, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17560150, Length=264, Percent_Identity=25.3787878787879, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17564282, Length=196, Percent_Identity=26.0204081632653, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71992402, Length=211, Percent_Identity=27.4881516587678, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17562990, Length=268, Percent_Identity=25.7462686567164, Blast_Score=64, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17562910, Length=272, Percent_Identity=25.7352941176471, Blast_Score=64, Evalue=1e-10,
Organism=Drosophila melanogaster, GI23397609, Length=258, Percent_Identity=31.7829457364341, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21355319, Length=264, Percent_Identity=28.4090909090909, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28571526, Length=255, Percent_Identity=29.4117647058824, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21357041, Length=255, Percent_Identity=27.843137254902, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24639444, Length=264, Percent_Identity=28.030303030303, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24644339, Length=260, Percent_Identity=25, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24643142, Length=258, Percent_Identity=25.1937984496124, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI161076371, Length=217, Percent_Identity=29.4930875576037, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24644337, Length=263, Percent_Identity=26.615969581749, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21358495, Length=188, Percent_Identity=25.531914893617, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 27638; Mature: 27638

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDLSGKLAIVSGSTAGIGLGISQSLAEAGATVVVIGRDAAKVEQALTSIRDKVPGAQL
CEEECCCCEEEEECCCCCCCCCHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCHHH
RGLTADLGTAEGAQKLFAAEPKADILVNNLGIYNAVDFFDAPDDEWTRFYEINVISGVRL
CCCCCCCCCCHHHHHHHHCCCCHHEEEECCCCHHHHHCCCCCCCCCEEEEEEEEECCCCH
SRHYVPAMVEQGWGRVIFLSSESGVATPADMINYGVTKSANLAVSHGLAKRLAGTGVTVN
HHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEE
AILPGPTFTDGVEEMLKDAAAESGRSLRDEADAFVRRARPSSIIQRVADVEEVAHLVTYI
EEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ASPLSSATTGAALRVDGGVVDSLTI
HHHHCCCCCCCEEEECCCCCCCCCC
>Mature Secondary Structure
MKIDLSGKLAIVSGSTAGIGLGISQSLAEAGATVVVIGRDAAKVEQALTSIRDKVPGAQL
CEEECCCCEEEEECCCCCCCCCHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCHHH
RGLTADLGTAEGAQKLFAAEPKADILVNNLGIYNAVDFFDAPDDEWTRFYEINVISGVRL
CCCCCCCCCCHHHHHHHHCCCCHHEEEECCCCHHHHHCCCCCCCCCEEEEEEEEECCCCH
SRHYVPAMVEQGWGRVIFLSSESGVATPADMINYGVTKSANLAVSHGLAKRLAGTGVTVN
HHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEE
AILPGPTFTDGVEEMLKDAAAESGRSLRDEADAFVRRARPSSIIQRVADVEEVAHLVTYI
EEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ASPLSSATTGAALRVDGGVVDSLTI
HHHHCCCCCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]