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The map label for this gene is lpdV [H]

Identifier: 77459688

GI number: 77459688

Start: 3950899

End: 3952272

Strand: Direct

Name: lpdV [H]

Synonym: Pfl01_3466

Alternate gene names: 77459688

Gene position: 3950899-3952272 (Clockwise)

Preceding gene: 77459687

Following gene: 77459689

Centisome position: 61.36

GC content: 62.45

Gene sequence:

>1374_bases
ATGCAATCTCTGAACACCACGCTGCTGATCATCGGCGGCGGTCCTGGCGGTTACGTGACGGCCATTCGTGCCGGGCAACT
GGGCATTTCGACCATTCTGGTCGAGGGTGAATCGCTGGGCGGCACCTGCCTGAACATCGGCTGCATTCCGTCGAAAGCGC
TGATCCACGTGGCCGAGCAGTTTCACCAGACGCAACATCACAACCAGCATTCGGCACTGGGCATCAGCGTTTCGGCGCCT
ACCCTCGACATCACTAAAAGCGTCGAGTGGAAGGACGGCATCGTTGATCGCCTGACCACCGGCGTCGCCGCGCTGCTGAA
GAAGAACAAGGTTCAGGTCATCAACGGCTGGGCCAAGGTCATCGACGGCAAGACCGTGGAAGTCGGCGACACCCGCATCC
AGTGTGAACATCTGGTGCTGGCCACCGGCTCGAAAAGCGTCAATCTGCCGATCCTGCCGATTGGCGGGCCGATCATATCG
TCCACCGAGGCGTTGGCGCCGAAGTCGGTGCCGAAACGCCTGATCGTGGTCGGCGGTGGTTACATCGGTCTGGAATTGGG
CATTGCCTATCGCAAGCTCGGCGCCGAGGTCAGTGTGGTCGAGGCGCAGGATCGGATCCTGCCGGCCTACGACGCCGAAC
TGACTCAACCGGTGCACGACGCGCTGAAGCAACTGGGCGTGAAGCTCTACCTCAAGCACAGCGTGCTGGGTTTCGACGGT
ACGTTGCAGGTGCGCGATCCGAACGGTGACACGCTGAATCTGGAAACCGATCAGGTGCTGGTGGCCGTCGGTCGCAAACC
CAATACCCAGGGCTGGAACCTTGAAGCGCTGAACCTGGACATGAACGGCTCGGCGATCAAGATTGACAGCCGCTGCCAGA
CCAGCATGCGTAATGTCTACGCCATCGGCGACCTGAGCGGCGAGCCGATGCTGGCCCACCGCGCCATGGCCCAGGGCGAA
ATGGTCGCCGAGCTGATCAGCGGCAAGACCCGCGAATTCAACCCGACCGCCATCGCTGCCGTGTGCTTTACCGACCCGGA
ACTGGTGGTCGTCGGCAAGACGCCGGACGAGGCCAAGGCGGCGGGACTTGACTGCATCGTGTCGAACTTCCCGTTCGCGG
CCAATGGCCGGGCGATGACGCTGGAATCGAAAACCGGCTTCGTGCGGGTGGTCGCCCGTCGAGACAATCATGTGATTGTC
GGCTGGCAGGCGGTCGGTGTCGGGGTCTCGGAGCTGTCGACTGCGTTCGCGCAAAGCCTGGAAATGGGCGCGCGACTGGA
AGACATCGGCGGCACCATCCATGCACACCCGACTCTGGGTGAAGCGGTGCAGGAAGCGGCGTTGCGTGCGCTTGGGCACG
CGTTGCACCTGTAA

Upstream 100 bases:

>100_bases
TTCGATCACCGCGTGGTCGACGGGATGGACGCGGCGCTCTTCATCCAGGCCATTCGTGGTCTGCTCGAACAACCCGCGAC
CCTGTTTGTGGAGTGATGGC

Downstream 100 bases:

>100_bases
AAACCCGACACGGGGGAGTTTTTTCATAAGCAGGCTAATAAATCTGCTGCTCCCCCATCGCCCGGGCTGCGAAACCGCTC
AAGAATGAAGTATTGTTGTG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of branched-chain alpha-keto acid dehydrogenase complex; LPD-Val [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MQSLNTTLLIIGGGPGGYVTAIRAGQLGISTILVEGESLGGTCLNIGCIPSKALIHVAEQFHQTQHHNQHSALGISVSAP
TLDITKSVEWKDGIVDRLTTGVAALLKKNKVQVINGWAKVIDGKTVEVGDTRIQCEHLVLATGSKSVNLPILPIGGPIIS
STEALAPKSVPKRLIVVGGGYIGLELGIAYRKLGAEVSVVEAQDRILPAYDAELTQPVHDALKQLGVKLYLKHSVLGFDG
TLQVRDPNGDTLNLETDQVLVAVGRKPNTQGWNLEALNLDMNGSAIKIDSRCQTSMRNVYAIGDLSGEPMLAHRAMAQGE
MVAELISGKTREFNPTAIAAVCFTDPELVVVGKTPDEAKAAGLDCIVSNFPFAANGRAMTLESKTGFVRVVARRDNHVIV
GWQAVGVGVSELSTAFAQSLEMGARLEDIGGTIHAHPTLGEAVQEAALRALGHALHL

Sequences:

>Translated_457_residues
MQSLNTTLLIIGGGPGGYVTAIRAGQLGISTILVEGESLGGTCLNIGCIPSKALIHVAEQFHQTQHHNQHSALGISVSAP
TLDITKSVEWKDGIVDRLTTGVAALLKKNKVQVINGWAKVIDGKTVEVGDTRIQCEHLVLATGSKSVNLPILPIGGPIIS
STEALAPKSVPKRLIVVGGGYIGLELGIAYRKLGAEVSVVEAQDRILPAYDAELTQPVHDALKQLGVKLYLKHSVLGFDG
TLQVRDPNGDTLNLETDQVLVAVGRKPNTQGWNLEALNLDMNGSAIKIDSRCQTSMRNVYAIGDLSGEPMLAHRAMAQGE
MVAELISGKTREFNPTAIAAVCFTDPELVVVGKTPDEAKAAGLDCIVSNFPFAANGRAMTLESKTGFVRVVARRDNHVIV
GWQAVGVGVSELSTAFAQSLEMGARLEDIGGTIHAHPTLGEAVQEAALRALGHALHL
>Mature_457_residues
MQSLNTTLLIIGGGPGGYVTAIRAGQLGISTILVEGESLGGTCLNIGCIPSKALIHVAEQFHQTQHHNQHSALGISVSAP
TLDITKSVEWKDGIVDRLTTGVAALLKKNKVQVINGWAKVIDGKTVEVGDTRIQCEHLVLATGSKSVNLPILPIGGPIIS
STEALAPKSVPKRLIVVGGGYIGLELGIAYRKLGAEVSVVEAQDRILPAYDAELTQPVHDALKQLGVKLYLKHSVLGFDG
TLQVRDPNGDTLNLETDQVLVAVGRKPNTQGWNLEALNLDMNGSAIKIDSRCQTSMRNVYAIGDLSGEPMLAHRAMAQGE
MVAELISGKTREFNPTAIAAVCFTDPELVVVGKTPDEAKAAGLDCIVSNFPFAANGRAMTLESKTGFVRVVARRDNHVIV
GWQAVGVGVSELSTAFAQSLEMGARLEDIGGTIHAHPTLGEAVQEAALRALGHALHL

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=465, Percent_Identity=36.989247311828, Blast_Score=288, Evalue=1e-77,
Organism=Homo sapiens, GI50301238, Length=456, Percent_Identity=28.0701754385965, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI22035672, Length=437, Percent_Identity=28.1464530892449, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI291045266, Length=444, Percent_Identity=24.5495495495495, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI291045268, Length=436, Percent_Identity=23.8532110091743, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI33519430, Length=426, Percent_Identity=23.0046948356808, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI33519428, Length=426, Percent_Identity=23.0046948356808, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI33519426, Length=426, Percent_Identity=23.0046948356808, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI148277065, Length=426, Percent_Identity=23.0046948356808, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI148277071, Length=426, Percent_Identity=23.0046948356808, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI1786307, Length=471, Percent_Identity=37.791932059448, Blast_Score=305, Evalue=3e-84,
Organism=Escherichia coli, GI1789915, Length=430, Percent_Identity=30, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI87082354, Length=470, Percent_Identity=26.5957446808511, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI87081717, Length=449, Percent_Identity=26.2806236080178, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1789065, Length=199, Percent_Identity=29.1457286432161, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=458, Percent_Identity=37.5545851528384, Blast_Score=283, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI71983419, Length=435, Percent_Identity=27.3563218390805, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71983429, Length=433, Percent_Identity=27.7136258660508, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17557007, Length=469, Percent_Identity=27.2921108742004, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=438, Percent_Identity=22.3744292237443, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6321091, Length=472, Percent_Identity=36.4406779661017, Blast_Score=263, Evalue=5e-71,
Organism=Saccharomyces cerevisiae, GI6325166, Length=457, Percent_Identity=27.3522975929978, Blast_Score=153, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6325240, Length=470, Percent_Identity=26.3829787234043, Blast_Score=144, Evalue=3e-35,
Organism=Drosophila melanogaster, GI21358499, Length=459, Percent_Identity=38.9978213507625, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24640553, Length=477, Percent_Identity=27.2536687631027, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24640549, Length=477, Percent_Identity=27.2536687631027, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24640551, Length=477, Percent_Identity=27.2536687631027, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI17737741, Length=463, Percent_Identity=25.7019438444924, Blast_Score=112, Evalue=5e-25,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 48266; Mature: 48266

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSLNTTLLIIGGGPGGYVTAIRAGQLGISTILVEGESLGGTCLNIGCIPSKALIHVAEQ
CCCCCEEEEEEECCCCCEEEEEEECCCCEEEEEEECCCCCCEEEEEECCCHHHHHHHHHH
FHQTQHHNQHSALGISVSAPTLDITKSVEWKDGIVDRLTTGVAALLKKNKVQVINGWAKV
HHHHHCCCCCCEEEEEECCCCEEEECCCCHHCCHHHHHHHHHHHHHHCCCEEEEECHHHH
IDGKTVEVGDTRIQCEHLVLATGSKSVNLPILPIGGPIISSTEALAPKSVPKRLIVVGGG
HCCCEEEECCCEEEEEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEECC
YIGLELGIAYRKLGAEVSVVEAQDRILPAYDAELTQPVHDALKQLGVKLYLKHSVLGFDG
EEEEEECHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEEEEECCCC
TLQVRDPNGDTLNLETDQVLVAVGRKPNTQGWNLEALNLDMNGSAIKIDSRCQTSMRNVY
EEEEECCCCCEEEEECCEEEEEECCCCCCCCCEEEEEEEECCCCEEEECHHHHHHHCCEE
AIGDLSGEPMLAHRAMAQGEMVAELISGKTREFNPTAIAAVCFTDPELVVVGKTPDEAKA
EEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCEEEEECCCCHHHH
AGLDCIVSNFPFAANGRAMTLESKTGFVRVVARRDNHVIVGWQAVGVGVSELSTAFAQSL
CCCEEEECCCCCCCCCEEEEEECCCCEEEEEEECCCEEEEEEEEECCCHHHHHHHHHHHH
EMGARLEDIGGTIHAHPTLGEAVQEAALRALGHALHL
HHCCEEHHCCCEEEECCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQSLNTTLLIIGGGPGGYVTAIRAGQLGISTILVEGESLGGTCLNIGCIPSKALIHVAEQ
CCCCCEEEEEEECCCCCEEEEEEECCCCEEEEEEECCCCCCEEEEEECCCHHHHHHHHHH
FHQTQHHNQHSALGISVSAPTLDITKSVEWKDGIVDRLTTGVAALLKKNKVQVINGWAKV
HHHHHCCCCCCEEEEEECCCCEEEECCCCHHCCHHHHHHHHHHHHHHCCCEEEEECHHHH
IDGKTVEVGDTRIQCEHLVLATGSKSVNLPILPIGGPIISSTEALAPKSVPKRLIVVGGG
HCCCEEEECCCEEEEEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEECC
YIGLELGIAYRKLGAEVSVVEAQDRILPAYDAELTQPVHDALKQLGVKLYLKHSVLGFDG
EEEEEECHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEEEEECCCC
TLQVRDPNGDTLNLETDQVLVAVGRKPNTQGWNLEALNLDMNGSAIKIDSRCQTSMRNVY
EEEEECCCCCEEEEECCEEEEEECCCCCCCCCEEEEEEEECCCCEEEECHHHHHHHCCEE
AIGDLSGEPMLAHRAMAQGEMVAELISGKTREFNPTAIAAVCFTDPELVVVGKTPDEAKA
EEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCEEEEECCCCHHHH
AGLDCIVSNFPFAANGRAMTLESKTGFVRVVARRDNHVIVGWQAVGVGVSELSTAFAQSL
CCCEEEECCCCCCCCCEEEEEECCCCEEEEEEECCCEEEEEEEEECCCHHHHHHHHHHHH
EMGARLEDIGGTIHAHPTLGEAVQEAALRALGHALHL
HHCCEEHHCCCEEEECCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3046941; 2917566; 1325638 [H]