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The map label for this gene is bkdB [H]

Identifier: 77459687

GI number: 77459687

Start: 3949623

End: 3950894

Strand: Direct

Name: bkdB [H]

Synonym: Pfl01_3465

Alternate gene names: 77459687

Gene position: 3949623-3950894 (Clockwise)

Preceding gene: 77459686

Following gene: 77459688

Centisome position: 61.34

GC content: 63.05

Gene sequence:

>1272_bases
ATGGGCACGCACGTTATCAAGATGCCGGACATCGGAGAAGGCATCGCAGAAGTAGAACTGTCGCAGTGGCACGTCAAGGT
TGGCGACCTGGTCGTTGAAGATCAGGTGCTGGCGGACGTGATGACCGACAAGGCGATGGTCGACATTCCGTCGCCGGTCC
ACGGCAAGGTGATTGCACTCGGCGGTCAGCCGGGCGAAGTGATGGCGGTCGGCAGTATTCTGATCAGCATCGAGGTTGAA
GGCGCCGGCAATCTGAAGGAGTCCGACAAACCGGCGCCTGTTGCGGCGAAAGAAACACCCGTCGCGCCAAAAGTTGAAGC
TGTCGTTGAAAGCAAACCCGCCGCACCACGTACGGCTCCGGTTTGCCAGGGCCCGATGGTTGCTCGCCAAGCCGATGAGC
GCCCACTGGCCTCGCCGGCCGTGCGCAAACATGCGCTGGATCTGGGCATTCAATTGCGTCTGGTGCGCGGCTCCGGCCCG
GCCGGCCGCGTGCTGCACGAAGACCTCGACGCCTATCTGGCGCAGGGTCAGTCGAATGCTTCGGCGCCGGTCGCCGCTGC
GTACGCCCAGCGTAATGATGAAGAACAGATTCAAGTGATCGGCATGCGCCGCAAGATTGCCCAGCGCATGCAGGACGCCA
CCCAGCGTGCGGCACACTTCAGTTATGTCGAAGAAATTGACGTCACCGCGATTGAAGAACTGCGCGCCCATTTGAACGAA
AAACACGGCGCGAGCCGTGGCAAGCTGACCTTACTGCCGTTCCTGGTGCGCGCACTGGTCGTCGCCCTGCGCGATTTCCC
GCAGATGAACGCCCGTTACGACGACGAAGCCCAGGTCATCACCCGCCTCGGCGCGGTGCACGTCGGCGTCGCCACCCAAA
GCGACGTCGGCCTGATGGTGCCGGTGGTGCGTCACGCCGAAGCGCGCAGCCTGTGGGACAGCGCGGCGGAAATCTCCCGC
CTGGCCAACGCCGCCCGCAATGGCAAGGCCAGCCGCGATGAACTGTCCGGCTCGACCATCACCCTGACCAGCCTCGGCGC
CCTTGGCGGCATCGTCAGTACCCCGGTGCTGAACCTGCCGGAAGTGGCCATCGTCGGCGTGAACAAAATCGTCGAACGGC
CAATGGTCGTCAAAGGCCAGGTGGTGATCCGCAAGATGATGAACCTCTCCAGCTCCTTCGATCACCGCGTGGTCGACGGG
ATGGACGCGGCGCTCTTCATCCAGGCCATTCGTGGTCTGCTCGAACAACCCGCGACCCTGTTTGTGGAGTGA

Upstream 100 bases:

>100_bases
CACCGGTTGGGACACCCCCTACCCGCACGCGCAGGAGTGGGCGTATTTCCCTGGGCCGTCCCGAGTGGGCGCGGCGCTGA
AACGGGTCATGGAGGTCTGA

Downstream 100 bases:

>100_bases
TGGCATGCAATCTCTGAACACCACGCTGCTGATCATCGGCGGCGGTCCTGGCGGTTACGTGACGGCCATTCGTGCCGGGC
AACTGGGCATTTCGACCATT

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MGTHVIKMPDIGEGIAEVELSQWHVKVGDLVVEDQVLADVMTDKAMVDIPSPVHGKVIALGGQPGEVMAVGSILISIEVE
GAGNLKESDKPAPVAAKETPVAPKVEAVVESKPAAPRTAPVCQGPMVARQADERPLASPAVRKHALDLGIQLRLVRGSGP
AGRVLHEDLDAYLAQGQSNASAPVAAAYAQRNDEEQIQVIGMRRKIAQRMQDATQRAAHFSYVEEIDVTAIEELRAHLNE
KHGASRGKLTLLPFLVRALVVALRDFPQMNARYDDEAQVITRLGAVHVGVATQSDVGLMVPVVRHAEARSLWDSAAEISR
LANAARNGKASRDELSGSTITLTSLGALGGIVSTPVLNLPEVAIVGVNKIVERPMVVKGQVVIRKMMNLSSSFDHRVVDG
MDAALFIQAIRGLLEQPATLFVE

Sequences:

>Translated_423_residues
MGTHVIKMPDIGEGIAEVELSQWHVKVGDLVVEDQVLADVMTDKAMVDIPSPVHGKVIALGGQPGEVMAVGSILISIEVE
GAGNLKESDKPAPVAAKETPVAPKVEAVVESKPAAPRTAPVCQGPMVARQADERPLASPAVRKHALDLGIQLRLVRGSGP
AGRVLHEDLDAYLAQGQSNASAPVAAAYAQRNDEEQIQVIGMRRKIAQRMQDATQRAAHFSYVEEIDVTAIEELRAHLNE
KHGASRGKLTLLPFLVRALVVALRDFPQMNARYDDEAQVITRLGAVHVGVATQSDVGLMVPVVRHAEARSLWDSAAEISR
LANAARNGKASRDELSGSTITLTSLGALGGIVSTPVLNLPEVAIVGVNKIVERPMVVKGQVVIRKMMNLSSSFDHRVVDG
MDAALFIQAIRGLLEQPATLFVE
>Mature_422_residues
GTHVIKMPDIGEGIAEVELSQWHVKVGDLVVEDQVLADVMTDKAMVDIPSPVHGKVIALGGQPGEVMAVGSILISIEVEG
AGNLKESDKPAPVAAKETPVAPKVEAVVESKPAAPRTAPVCQGPMVARQADERPLASPAVRKHALDLGIQLRLVRGSGPA
GRVLHEDLDAYLAQGQSNASAPVAAAYAQRNDEEQIQVIGMRRKIAQRMQDATQRAAHFSYVEEIDVTAIEELRAHLNEK
HGASRGKLTLLPFLVRALVVALRDFPQMNARYDDEAQVITRLGAVHVGVATQSDVGLMVPVVRHAEARSLWDSAAEISRL
ANAARNGKASRDELSGSTITLTSLGALGGIVSTPVLNLPEVAIVGVNKIVERPMVVKGQVVIRKMMNLSSSFDHRVVDGM
DAALFIQAIRGLLEQPATLFVE

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=442, Percent_Identity=31.6742081447964, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI19923748, Length=226, Percent_Identity=33.1858407079646, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI31711992, Length=439, Percent_Identity=26.1958997722096, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI203098816, Length=232, Percent_Identity=26.2931034482759, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI203098753, Length=232, Percent_Identity=26.2931034482759, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=27.9503105590062, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1786946, Length=424, Percent_Identity=31.3679245283019, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI1786305, Length=429, Percent_Identity=29.8368298368298, Blast_Score=178, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI17537937, Length=432, Percent_Identity=32.6388888888889, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI25146366, Length=421, Percent_Identity=29.9287410926366, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17560088, Length=424, Percent_Identity=28.3018867924528, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17538894, Length=310, Percent_Identity=23.8709677419355, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320352, Length=437, Percent_Identity=28.8329519450801, Blast_Score=186, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6324258, Length=449, Percent_Identity=25.8351893095768, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI18859875, Length=436, Percent_Identity=28.6697247706422, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24645909, Length=231, Percent_Identity=33.3333333333333, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24582497, Length=292, Percent_Identity=30.1369863013699, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI20129315, Length=435, Percent_Identity=27.816091954023, Blast_Score=112, Evalue=4e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 45156; Mature: 45024

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTHVIKMPDIGEGIAEVELSQWHVKVGDLVVEDQVLADVMTDKAMVDIPSPVHGKVIAL
CCCEEEECCCCCCCHHHEEHHHHEEEECCEEEHHHHHHHHHHCCCEEECCCCCCCEEEEE
GGQPGEVMAVGSILISIEVEGAGNLKESDKPAPVAAKETPVAPKVEAVVESKPAAPRTAP
CCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCC
VCQGPMVARQADERPLASPAVRKHALDLGIQLRLVRGSGPAGRVLHEDLDAYLAQGQSNA
CCCCCHHHHCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCC
SAPVAAAYAQRNDEEQIQVIGMRRKIAQRMQDATQRAAHFSYVEEIDVTAIEELRAHLNE
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KHGASRGKLTLLPFLVRALVVALRDFPQMNARYDDEAQVITRLGAVHVGVATQSDVGLMV
HCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHEEEEECCCCCCCHH
PVVRHAEARSLWDSAAEISRLANAARNGKASRDELSGSTITLTSLGALGGIVSTPVLNLP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEEHHHHHHHHHHCCCCCCC
EVAIVGVNKIVERPMVVKGQVVIRKMMNLSSSFDHRVVDGMDAALFIQAIRGLLEQPATL
CEEEECHHHHHCCCCEEHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCE
FVE
ECC
>Mature Secondary Structure 
GTHVIKMPDIGEGIAEVELSQWHVKVGDLVVEDQVLADVMTDKAMVDIPSPVHGKVIAL
CCEEEECCCCCCCHHHEEHHHHEEEECCEEEHHHHHHHHHHCCCEEECCCCCCCEEEEE
GGQPGEVMAVGSILISIEVEGAGNLKESDKPAPVAAKETPVAPKVEAVVESKPAAPRTAP
CCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCC
VCQGPMVARQADERPLASPAVRKHALDLGIQLRLVRGSGPAGRVLHEDLDAYLAQGQSNA
CCCCCHHHHCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCC
SAPVAAAYAQRNDEEQIQVIGMRRKIAQRMQDATQRAAHFSYVEEIDVTAIEELRAHLNE
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KHGASRGKLTLLPFLVRALVVALRDFPQMNARYDDEAQVITRLGAVHVGVATQSDVGLMV
HCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHEEEEECCCCCCCHH
PVVRHAEARSLWDSAAEISRLANAARNGKASRDELSGSTITLTSLGALGGIVSTPVLNLP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEEHHHHHHHHHHCCCCCCC
EVAIVGVNKIVERPMVVKGQVVIRKMMNLSSSFDHRVVDGMDAALFIQAIRGLLEQPATL
CEEEECHHHHHCCCCEEHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCE
FVE
ECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3046941 [H]