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The map label for this gene is bigR [H]

Identifier: 77459681

GI number: 77459681

Start: 3944367

End: 3944705

Strand: Reverse

Name: bigR [H]

Synonym: Pfl01_3459

Alternate gene names: 77459681

Gene position: 3944705-3944367 (Counterclockwise)

Preceding gene: 77459684

Following gene: 77459680

Centisome position: 61.27

GC content: 59.88

Gene sequence:

>339_bases
ATGCAATCCAGTCTGACCGAATGTGAAGTGGCGCAACTGCGCGCCTCGGCCTCCAAGGCCTGTTCGCTGCTCAAGGCCTT
GGCCAACGAGGATCGGCTGTTGATCCTGTGCCAGTTGACCCAGGGCGAGCGCAACGTCGGCGAACTGGAGAAAATGACCG
GCGTGCGTCAGCCGACCCTGTCCCAGCAACTGGGCATCCTGCGCGATGAAGGGCTGGTCGCGACCCGTCGTGAAGGCAAG
TACATTTTTTACGGTCTCGCCAGTCACGAAGTGATCCAGGTGATGAAAACCCTCTCGGGACTCTACTGCGGAGCCGTGCT
CAAAAGCTGGGCGCAATAG

Upstream 100 bases:

>100_bases
ATGGACACGGTCCTGTGCAGAAGGTGGAAAGTCAGAGTTGCAACACTTTATGTAAACACATAATGTCCGCAGCTTAAGTG
ACGTTGAAGGCATCCTGCAA

Downstream 100 bases:

>100_bases
ACCCGGCTATCCTTGCGTGCAGCCATAAGGAACAAACAATGAACGATCAACACTGGGGCCCATCCATCAGTGCGGACATC
GTGGTCATCGGCGGCGGCAC

Product: ArsR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 112; Mature: 112

Protein sequence:

>112_residues
MQSSLTECEVAQLRASASKACSLLKALANEDRLLILCQLTQGERNVGELEKMTGVRQPTLSQQLGILRDEGLVATRREGK
YIFYGLASHEVIQVMKTLSGLYCGAVLKSWAQ

Sequences:

>Translated_112_residues
MQSSLTECEVAQLRASASKACSLLKALANEDRLLILCQLTQGERNVGELEKMTGVRQPTLSQQLGILRDEGLVATRREGK
YIFYGLASHEVIQVMKTLSGLYCGAVLKSWAQ
>Mature_112_residues
MQSSLTECEVAQLRASASKACSLLKALANEDRLLILCQLTQGERNVGELEKMTGVRQPTLSQQLGILRDEGLVATRREGK
YIFYGLASHEVIQVMKTLSGLYCGAVLKSWAQ

Specific function: Represses an operon that comprises at least itself and blh. Binds to a palindromic AT-rich sequence spanning the -10 region of the blh promoter and blocks transcription of the operon [H]

COG id: COG0640

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH arsR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789021, Length=96, Percent_Identity=41.6666666666667, Blast_Score=79, Evalue=6e-17,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001845
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01022 HTH_5 [H]

EC number: NA

Molecular weight: Translated: 12316; Mature: 12316

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS50987 HTH_ARSR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSSLTECEVAQLRASASKACSLLKALANEDRLLILCQLTQGERNVGELEKMTGVRQPTL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCH
SQQLGILRDEGLVATRREGKYIFYGLASHEVIQVMKTLSGLYCGAVLKSWAQ
HHHHCCCCCCCEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQSSLTECEVAQLRASASKACSLLKALANEDRLLILCQLTQGERNVGELEKMTGVRQPTL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCH
SQQLGILRDEGLVATRREGKYIFYGLASHEVIQVMKTLSGLYCGAVLKSWAQ
HHHHCCCCCCCEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]