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The map label for this gene is cynR [H]

Identifier: 77459590

GI number: 77459590

Start: 3844181

End: 3845059

Strand: Reverse

Name: cynR [H]

Synonym: Pfl01_3368

Alternate gene names: 77459590

Gene position: 3845059-3844181 (Counterclockwise)

Preceding gene: 77459594

Following gene: 77459588

Centisome position: 59.72

GC content: 62.12

Gene sequence:

>879_bases
ATGCTGCTGCGACATTTGCGATACCTGCTGGCTGTGGCCGATCACGGCGGCTTCACCCGGGCGGCCGAGGCGCTGCACGT
GTCGCAGCCGACCTTGTCGCAACAGATCCGCCAACTGGAAGAAAGCCTCGGTGTGGATCTGTTCGACCGCACCTCGCGCT
CGGTCAAACCCACTGACGCCGGCCAGGCCTATATCGAATGCGCACGACGGGTACTGGTGGAACTGGAGGCGGGCAAGCGC
GCGTTGCATGACGTGAAGGACCTGTCCCGAGGCTCGCTGCGCCTGGCTATGACACCGACGTTCATGGCGTATCTGGTCGG
GCCGCTGGTGCGCGATTACGTGGCGCGGTATCCGGGGATTCACCTGGAGATTTTCGAGTTGTCGATGGACGACATCGAGG
CCGGGCTGGCGGATGACTCGCTGGATATCGCGATTGCCTTCACGCCGGTGCGCAATCCGGACATTGAATGCATTCCGGCA
TTTGTCGAGACGTTGGGGGTGATGGTCGGGCGCGAGCATCCGCTGTACGACAGCTCATCGGTGCTGACCCCGGATGACAT
TGCGCAGCTGGATTTCGCCTTGCTGGCGCCGGATTTCATCACGCGGCTATCGATTGACGAGTATTTCCGTCAGCGCGGCA
TCACGCCCAAGGTGCAGATCGAGGTGAACTCGGTGAGTACGTTGCTGGAAGTGATCCGGCATGCGCCGATTGCGACCATG
TTGCCGGAGGCGATTGCGACCGAGGACCGGGCGTTGCGCCGGTTGCATGTCGAAAGCGAGGCGCCGCAGCGGGGAGCGGC
GTTATTGCGGCGCCAGAACAACTATCACAGTGCGGCGGCTGAGGCGTTCATGAAGCTGGTGCTGGAAACTCGTTCATGA

Upstream 100 bases:

>100_bases
TGGCTTGCTGTGTGGGATTCATCTTCTGCCGATTGATCCATAGCGTCCAAGTCCGATATACAATGCAAAGCATAAGCACT
GCCTATAGATGGAGCGTCCC

Downstream 100 bases:

>100_bases
GCTTTTTTCGCGAGCAATAAAAAGGGCCTGCTTCGTTTTCGAAGCAGGCCCTTTTTATTGAAGTTTTAACTTAGCAGAAC
CGATCAATCACCCGAACTTC

Product: DNA-binding transcriptional regulator CynR

Products: NA

Alternate protein names: Cyn operon transcriptional activator [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MLLRHLRYLLAVADHGGFTRAAEALHVSQPTLSQQIRQLEESLGVDLFDRTSRSVKPTDAGQAYIECARRVLVELEAGKR
ALHDVKDLSRGSLRLAMTPTFMAYLVGPLVRDYVARYPGIHLEIFELSMDDIEAGLADDSLDIAIAFTPVRNPDIECIPA
FVETLGVMVGREHPLYDSSSVLTPDDIAQLDFALLAPDFITRLSIDEYFRQRGITPKVQIEVNSVSTLLEVIRHAPIATM
LPEAIATEDRALRRLHVESEAPQRGAALLRRQNNYHSAAAEAFMKLVLETRS

Sequences:

>Translated_292_residues
MLLRHLRYLLAVADHGGFTRAAEALHVSQPTLSQQIRQLEESLGVDLFDRTSRSVKPTDAGQAYIECARRVLVELEAGKR
ALHDVKDLSRGSLRLAMTPTFMAYLVGPLVRDYVARYPGIHLEIFELSMDDIEAGLADDSLDIAIAFTPVRNPDIECIPA
FVETLGVMVGREHPLYDSSSVLTPDDIAQLDFALLAPDFITRLSIDEYFRQRGITPKVQIEVNSVSTLLEVIRHAPIATM
LPEAIATEDRALRRLHVESEAPQRGAALLRRQNNYHSAAAEAFMKLVLETRS
>Mature_292_residues
MLLRHLRYLLAVADHGGFTRAAEALHVSQPTLSQQIRQLEESLGVDLFDRTSRSVKPTDAGQAYIECARRVLVELEAGKR
ALHDVKDLSRGSLRLAMTPTFMAYLVGPLVRDYVARYPGIHLEIFELSMDDIEAGLADDSLDIAIAFTPVRNPDIECIPA
FVETLGVMVGREHPLYDSSSVLTPDDIAQLDFALLAPDFITRLSIDEYFRQRGITPKVQIEVNSVSTLLEVIRHAPIATM
LPEAIATEDRALRRLHVESEAPQRGAALLRRQNNYHSAAAEAFMKLVLETRS

Specific function: Positively regulates the cynTSX operon, and negatively regulates its own transcription. Binds specifically to the cynR- cynTSX intergenic region [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=289, Percent_Identity=50.8650519031142, Blast_Score=293, Evalue=9e-81,
Organism=Escherichia coli, GI1788297, Length=249, Percent_Identity=32.9317269076305, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1787530, Length=236, Percent_Identity=28.3898305084746, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1788887, Length=231, Percent_Identity=32.034632034632, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1790399, Length=243, Percent_Identity=31.2757201646091, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1787879, Length=249, Percent_Identity=29.718875502008, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI145693105, Length=174, Percent_Identity=29.8850574712644, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1789173, Length=192, Percent_Identity=32.8125, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789204, Length=234, Percent_Identity=27.3504273504274, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87081904, Length=187, Percent_Identity=30.4812834224599, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI2367136, Length=262, Percent_Identity=29.3893129770992, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788748, Length=294, Percent_Identity=26.8707482993197, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786401, Length=158, Percent_Identity=29.746835443038, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790262, Length=231, Percent_Identity=24.2424242424242, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788296, Length=200, Percent_Identity=28, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1789639, Length=144, Percent_Identity=29.8611111111111, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787601, Length=278, Percent_Identity=21.5827338129496, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787589, Length=130, Percent_Identity=30.7692307692308, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787949, Length=280, Percent_Identity=23.9285714285714, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1790208, Length=249, Percent_Identity=26.5060240963855, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787806, Length=245, Percent_Identity=22.4489795918367, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87082132, Length=252, Percent_Identity=25.7936507936508, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI145693193, Length=141, Percent_Identity=32.6241134751773, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI87081978, Length=144, Percent_Identity=31.9444444444444, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32541; Mature: 32541

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLRHLRYLLAVADHGGFTRAAEALHVSQPTLSQQIRQLEESLGVDLFDRTSRSVKPTDA
CHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCC
GQAYIECARRVLVELEAGKRALHDVKDLSRGSLRLAMTPTFMAYLVGPLVRDYVARYPGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCC
HLEIFELSMDDIEAGLADDSLDIAIAFTPVRNPDIECIPAFVETLGVMVGREHPLYDSSS
EEEEEECCHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
VLTPDDIAQLDFALLAPDFITRLSIDEYFRQRGITPKVQIEVNSVSTLLEVIRHAPIATM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHCCHHHH
LPEAIATEDRALRRLHVESEAPQRGAALLRRQNNYHSAAAEAFMKLVLETRS
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLLRHLRYLLAVADHGGFTRAAEALHVSQPTLSQQIRQLEESLGVDLFDRTSRSVKPTDA
CHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCC
GQAYIECARRVLVELEAGKRALHDVKDLSRGSLRLAMTPTFMAYLVGPLVRDYVARYPGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCC
HLEIFELSMDDIEAGLADDSLDIAIAFTPVRNPDIECIPAFVETLGVMVGREHPLYDSSS
EEEEEECCHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
VLTPDDIAQLDFALLAPDFITRLSIDEYFRQRGITPKVQIEVNSVSTLLEVIRHAPIATM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHCCHHHH
LPEAIATEDRALRRLHVESEAPQRGAALLRRQNNYHSAAAEAFMKLVLETRS
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]