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The map label for this gene is gudD [H]

Identifier: 77459505

GI number: 77459505

Start: 3768470

End: 3769744

Strand: Reverse

Name: gudD [H]

Synonym: Pfl01_3283

Alternate gene names: 77459505

Gene position: 3769744-3768470 (Counterclockwise)

Preceding gene: 77459506

Following gene: 77459503

Centisome position: 58.55

GC content: 60.47

Gene sequence:

>1275_bases
TTGAAAATCACTCGCGTAACCGTCACTCCGATTGCCTTCCGTGATCCGCCGCTGCTCAACGCCAGTGGCATCCACGAACC
GTTCGCCCTGCGCTCGATCATCGAGATCGAGAGTGACAACGGCTACATCGGCCTCGGCGAAAGCTATGGCGATGCCCCGG
CGCTGGCGATCCAGCAACAGGTGCAGTCGCAGTTGATCGGCCTCGATCCGTTTAATCTTAATCAGCTGCGTCGGATCGTT
CAGACCACGGTTGCCGCACACAAACCGGCCAGTCTGGCGGGTGCCGAACTGGCGCCCGGTTCCCACGCCAGCAAGGCCGT
CAGCAATGCGTATTCGGCGTTCGAAGTGGCGTTTCTCGACTTGCAGGCTCGTTACCTGAATGTGCCGTTGGTGGATCTGC
TGGGCGGGGCGGTGCGCGACGAAGTACCGTTCAGCGCCTACCTGTTTTTCAAGTACGCGCAACACGTCGATTCACCCTAC
AAACCGGACAACTGGGGCGAGGCGCTGAACGAACAGCAGATCGTCGCCCAGGCCGCGCGAATGATCGAGGCGTACGGTTT
CAAGAGCATCAAGCTCAAGGCCGGCACGTTGCCGCCGGAACATGAAGTGGCATGCATCAAGGCGCTGAAAAAGGCCTTCC
CCGGTTATCCGCTGCGCATCGATCCCAACGGCAACTGGTCGCTGGAAACCTCGATCCGCATGGCCGAGTTGCTGGGCGAC
GATCTGCAGTATTACGAAGACCCGACCCCGGGCCTCGAAGGAATGGCCGAGCTGCACAAACGCACCGGGCTGCCGCTGGC
CACCAACATGGTGGTCACTGATTTCGACGAGTTCCGCCGCAGCGTGGCGCAGAACAGCGTGCAGATCGTACTCGCCGATC
ACCATTACTGGGGCGGTCTGCGCGACACCCAGACTCTGGCGAAAATGTGTGACACCTTCGGCCTCGGCGTGTCGATGCAT
TCCAACTCACACCTGGGCATCAGCCTTATGGCCATGGCGCACGTGGCGGCTGCGGTACCGAATCTGGATTACGCGTGCGA
CACCCATTATCCGTGGCAGGAGCCGGATGAAGAGGTGATCAAGGGCGGCAAGCTGCCGATTGTCGATGGCTGCGTGAAGA
TCACGCGTGCGCCGGGCCTGGGGCTGGAACTGGATCACGATCAACTGGGCAAGCTGCATGACCAGTACCTGACCTGCGGC
ATTCGCCAGCGCGATGATGTGCGGCAGATGCAACGCTATAAACCTGACTGGAAAGCGCTGAAGCCACGATTCTGA

Upstream 100 bases:

>100_bases
CAAAGGCAACACCCCGGCAAAGCCTGAACCCGAAACCGCCGTTGCGCCGACGGCCAACCCGCTGGCGAGCCACTGATTCC
GACTCAACAGGAGCGTCATT

Downstream 100 bases:

>100_bases
TCGAGCAAAACCAGAGGGCGCCAAACGGCGCCCTCGGTTGTTTCAAAGCGTGCGCATCAACCAGTCGCGAAACGCCTTCA
GCGACGCCAGCGACTCATTG

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: GDH; GlucD [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MKITRVTVTPIAFRDPPLLNASGIHEPFALRSIIEIESDNGYIGLGESYGDAPALAIQQQVQSQLIGLDPFNLNQLRRIV
QTTVAAHKPASLAGAELAPGSHASKAVSNAYSAFEVAFLDLQARYLNVPLVDLLGGAVRDEVPFSAYLFFKYAQHVDSPY
KPDNWGEALNEQQIVAQAARMIEAYGFKSIKLKAGTLPPEHEVACIKALKKAFPGYPLRIDPNGNWSLETSIRMAELLGD
DLQYYEDPTPGLEGMAELHKRTGLPLATNMVVTDFDEFRRSVAQNSVQIVLADHHYWGGLRDTQTLAKMCDTFGLGVSMH
SNSHLGISLMAMAHVAAAVPNLDYACDTHYPWQEPDEEVIKGGKLPIVDGCVKITRAPGLGLELDHDQLGKLHDQYLTCG
IRQRDDVRQMQRYKPDWKALKPRF

Sequences:

>Translated_424_residues
MKITRVTVTPIAFRDPPLLNASGIHEPFALRSIIEIESDNGYIGLGESYGDAPALAIQQQVQSQLIGLDPFNLNQLRRIV
QTTVAAHKPASLAGAELAPGSHASKAVSNAYSAFEVAFLDLQARYLNVPLVDLLGGAVRDEVPFSAYLFFKYAQHVDSPY
KPDNWGEALNEQQIVAQAARMIEAYGFKSIKLKAGTLPPEHEVACIKALKKAFPGYPLRIDPNGNWSLETSIRMAELLGD
DLQYYEDPTPGLEGMAELHKRTGLPLATNMVVTDFDEFRRSVAQNSVQIVLADHHYWGGLRDTQTLAKMCDTFGLGVSMH
SNSHLGISLMAMAHVAAAVPNLDYACDTHYPWQEPDEEVIKGGKLPIVDGCVKITRAPGLGLELDHDQLGKLHDQYLTCG
IRQRDDVRQMQRYKPDWKALKPRF
>Mature_424_residues
MKITRVTVTPIAFRDPPLLNASGIHEPFALRSIIEIESDNGYIGLGESYGDAPALAIQQQVQSQLIGLDPFNLNQLRRIV
QTTVAAHKPASLAGAELAPGSHASKAVSNAYSAFEVAFLDLQARYLNVPLVDLLGGAVRDEVPFSAYLFFKYAQHVDSPY
KPDNWGEALNEQQIVAQAARMIEAYGFKSIKLKAGTLPPEHEVACIKALKKAFPGYPLRIDPNGNWSLETSIRMAELLGD
DLQYYEDPTPGLEGMAELHKRTGLPLATNMVVTDFDEFRRSVAQNSVQIVLADHHYWGGLRDTQTLAKMCDTFGLGVSMH
SNSHLGISLMAMAHVAAAVPNLDYACDTHYPWQEPDEEVIKGGKLPIVDGCVKITRAPGLGLELDHDQLGKLHDQYLTCG
IRQRDDVRQMQRYKPDWKALKPRF

Specific function: Catalyzes the dehydration of glucarate to 5-keto-4- deoxy-D-glucarate (5-kdGluc) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789150, Length=433, Percent_Identity=35.5658198614319, Blast_Score=238, Evalue=6e-64,
Organism=Escherichia coli, GI1789151, Length=443, Percent_Identity=35.8916478555305, Blast_Score=230, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.40 [H]

Molecular weight: Translated: 46873; Mature: 46873

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITRVTVTPIAFRDPPLLNASGIHEPFALRSIIEIESDNGYIGLGESYGDAPALAIQQQ
CEEEEEEECEEEECCCCCCCCCCCCCHHHHHHHEEEECCCCEEECCCCCCCCHHHHHHHH
VQSQLIGLDPFNLNQLRRIVQTTVAAHKPASLAGAELAPGSHASKAVSNAYSAFEVAFLD
HHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEE
LQARYLNVPLVDLLGGAVRDEVPFSAYLFFKYAQHVDSPYKPDNWGEALNEQQIVAQAAR
HHHHHCCCCHHHHHCCHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
MIEAYGFKSIKLKAGTLPPEHEVACIKALKKAFPGYPLRIDPNGNWSLETSIRMAELLGD
HHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEECCCCCEEEHHHHHHHHHHCC
DLQYYEDPTPGLEGMAELHKRTGLPLATNMVVTDFDEFRRSVAQNSVQIVLADHHYWGGL
HHHHHCCCCCCHHHHHHHHHHCCCCEECCCEEECHHHHHHHHHCCCEEEEEECCCCCCCC
RDTQTLAKMCDTFGLGVSMHSNSHLGISLMAMAHVAAAVPNLDYACDTHYPWQEPDEEVI
CHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
KGGKLPIVDGCVKITRAPGLGLELDHDQLGKLHDQYLTCGIRQRDDVRQMQRYKPDWKAL
CCCCCCEEHHHHHHHCCCCCCCEECHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHC
KPRF
CCCC
>Mature Secondary Structure
MKITRVTVTPIAFRDPPLLNASGIHEPFALRSIIEIESDNGYIGLGESYGDAPALAIQQQ
CEEEEEEECEEEECCCCCCCCCCCCCHHHHHHHEEEECCCCEEECCCCCCCCHHHHHHHH
VQSQLIGLDPFNLNQLRRIVQTTVAAHKPASLAGAELAPGSHASKAVSNAYSAFEVAFLD
HHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEE
LQARYLNVPLVDLLGGAVRDEVPFSAYLFFKYAQHVDSPYKPDNWGEALNEQQIVAQAAR
HHHHHCCCCHHHHHCCHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
MIEAYGFKSIKLKAGTLPPEHEVACIKALKKAFPGYPLRIDPNGNWSLETSIRMAELLGD
HHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEECCCCCEEEHHHHHHHHHHCC
DLQYYEDPTPGLEGMAELHKRTGLPLATNMVVTDFDEFRRSVAQNSVQIVLADHHYWGGL
HHHHHCCCCCCHHHHHHHHHHCCCCEECCCEEECHHHHHHHHHCCCEEEEEECCCCCCCC
RDTQTLAKMCDTFGLGVSMHSNSHLGISLMAMAHVAAAVPNLDYACDTHYPWQEPDEEVI
CHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
KGGKLPIVDGCVKITRAPGLGLELDHDQLGKLHDQYLTCGIRQRDDVRQMQRYKPDWKAL
CCCCCCEEHHHHHHHCCCCCCCEECHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHC
KPRF
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]