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The map label for this gene is potF [H]

Identifier: 77459482

GI number: 77459482

Start: 3741689

End: 3742789

Strand: Reverse

Name: potF [H]

Synonym: Pfl01_3260

Alternate gene names: 77459482

Gene position: 3742789-3741689 (Counterclockwise)

Preceding gene: 77459483

Following gene: 77459480

Centisome position: 58.13

GC content: 59.13

Gene sequence:

>1101_bases
ATGGTCACGATGAAACGCATTCTGGGCGCCACTGTTTTCGGGCTGACGCTGCTGGCCAGCGCCGTACAGGCCGAACAGCG
CGAACTGCGGGTGTACAACTGGGCGGATTACATTCTGCCGTCGGTGCCCAAGGATTTCGCCGCCAAAAGCAACATCAAAG
TGACCTGGGACACCTTCGATACCAACGAATCCCTCGAGGCCAAGTTGCTGACCGGTAACTCCGGCTATGACCTGGTGGTG
CCGTCGAACCAGTTCCTCGATACCCAGATCAAGGCTGGCGTGTTCCAGAAACTCGACAAGTCCAAGCTGCCGAACTGGAG
CCACCAGGACCCGGCGCTGCTCAAGTTGCTCGACGCCAACGACCCCGGCAATCAGTACGGCGTGCCCTACATGGTCGGCA
CGGTGCTGATCGGTTTCAACCCGGCCAAGGTCAAGGCTGCGCTCGGTGAGAATGCGCCGGTGGACAGCTGGGATCTGGTG
TTCAAGCCGGAGAACATGGAAAAGCTGAAATCCTGCGGCGTGGCGATGCTCGATTCGCCGTCGGAAATCCTGCCGCTGGC
TCTGCATTACCTGGGCCTCGACCCGAACAGCCAGAACCCGGCCGACTATGAGAAAGCCAAGGACCTGATGCTCAAGGTTC
GCCCTTACGTCACCTACTTCAACTCGGCCAAATACATGACCGACATCGCCAACGGCGATATCTGCGTGGCCATTGGTTAT
TCCGGCAGCTTCTACCAGTTCGGCAACCGTGCCAAAGAGGCGGGCAACGGCGTGGTGGTCGACTGGCGCTTGCCGAAGGA
AGGCGCGCCGATCTGGTTCGATACCTTCGCCATTCCGAAGAGCGCGAAGAACGTCGAAGAGGCCCACGAGTTCCTCAACA
CCTTGCTCGATCCGAAGGTCATCGCTTCGATCAGCGACTTCCTCGGTTACCCGAATGCCAACAAGGATTCGCTGCCGCTG
ATCAACAAGGAAATCACCGGCAACCCGAACCTGACGCCGACCAGCGAAGCGCTGAAGAAGCTGTACGTGGTGCAGCCACT
GCCGCAGAAACTGGAGCGGGTGCGGACCCGGGTCTGGACCAGCATCAAGTCGGATAAATAA

Upstream 100 bases:

>100_bases
CTAAATGCTGCACCTTCCTTGCCCTTGGCTGATTTGACAGCCGCTGCCGTGTACAGAACATAAAAACCATCGACTCACGC
TGCATTCGGGGAAACACAAC

Downstream 100 bases:

>100_bases
GCCCAAAACCGGGGAAGCGAACCACGTTCGCTCCCCCGGCTGTTATTTCGCCTGACTTTCTTCAGGCGATTTGTCCTTCG
TCAGGATCAGACTCAGCACC

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MVTMKRILGATVFGLTLLASAVQAEQRELRVYNWADYILPSVPKDFAAKSNIKVTWDTFDTNESLEAKLLTGNSGYDLVV
PSNQFLDTQIKAGVFQKLDKSKLPNWSHQDPALLKLLDANDPGNQYGVPYMVGTVLIGFNPAKVKAALGENAPVDSWDLV
FKPENMEKLKSCGVAMLDSPSEILPLALHYLGLDPNSQNPADYEKAKDLMLKVRPYVTYFNSAKYMTDIANGDICVAIGY
SGSFYQFGNRAKEAGNGVVVDWRLPKEGAPIWFDTFAIPKSAKNVEEAHEFLNTLLDPKVIASISDFLGYPNANKDSLPL
INKEITGNPNLTPTSEALKKLYVVQPLPQKLERVRTRVWTSIKSDK

Sequences:

>Translated_366_residues
MVTMKRILGATVFGLTLLASAVQAEQRELRVYNWADYILPSVPKDFAAKSNIKVTWDTFDTNESLEAKLLTGNSGYDLVV
PSNQFLDTQIKAGVFQKLDKSKLPNWSHQDPALLKLLDANDPGNQYGVPYMVGTVLIGFNPAKVKAALGENAPVDSWDLV
FKPENMEKLKSCGVAMLDSPSEILPLALHYLGLDPNSQNPADYEKAKDLMLKVRPYVTYFNSAKYMTDIANGDICVAIGY
SGSFYQFGNRAKEAGNGVVVDWRLPKEGAPIWFDTFAIPKSAKNVEEAHEFLNTLLDPKVIASISDFLGYPNANKDSLPL
INKEITGNPNLTPTSEALKKLYVVQPLPQKLERVRTRVWTSIKSDK
>Mature_366_residues
MVTMKRILGATVFGLTLLASAVQAEQRELRVYNWADYILPSVPKDFAAKSNIKVTWDTFDTNESLEAKLLTGNSGYDLVV
PSNQFLDTQIKAGVFQKLDKSKLPNWSHQDPALLKLLDANDPGNQYGVPYMVGTVLIGFNPAKVKAALGENAPVDSWDLV
FKPENMEKLKSCGVAMLDSPSEILPLALHYLGLDPNSQNPADYEKAKDLMLKVRPYVTYFNSAKYMTDIANGDICVAIGY
SGSFYQFGNRAKEAGNGVVVDWRLPKEGAPIWFDTFAIPKSAKNVEEAHEFLNTLLDPKVIASISDFLGYPNANKDSLPL
INKEITGNPNLTPTSEALKKLYVVQPLPQKLERVRTRVWTSIKSDK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=365, Percent_Identity=53.4246575342466, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1787367, Length=323, Percent_Identity=34.3653250773994, Blast_Score=178, Evalue=6e-46,
Organism=Escherichia coli, GI1787711, Length=297, Percent_Identity=26.2626262626263, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40565; Mature: 40565

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTMKRILGATVFGLTLLASAVQAEQRELRVYNWADYILPSVPKDFAAKSNIKVTWDTFD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHCCCCCHHHHCCCCCEEEEEECC
TNESLEAKLLTGNSGYDLVVPSNQFLDTQIKAGVFQKLDKSKLPNWSHQDPALLKLLDAN
CCCCCEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECC
DPGNQYGVPYMVGTVLIGFNPAKVKAALGENAPVDSWDLVFKPENMEKLKSCGVAMLDSP
CCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHCCEEEECCC
SEILPLALHYLGLDPNSQNPADYEKAKDLMLKVRPYVTYFNSAKYMTDIANGDICVAIGY
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHEEECHHEEEECCCHHEEECCCCCEEEEECC
SGSFYQFGNRAKEAGNGVVVDWRLPKEGAPIWFDTFAIPKSAKNVEEAHEFLNTLLDPKV
CCCHHHHCCHHHHCCCCEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCHHH
IASISDFLGYPNANKDSLPLINKEITGNPNLTPTSEALKKLYVVQPLPQKLERVRTRVWT
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHH
SIKSDK
HHCCCC
>Mature Secondary Structure
MVTMKRILGATVFGLTLLASAVQAEQRELRVYNWADYILPSVPKDFAAKSNIKVTWDTFD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHCCCCCHHHHCCCCCEEEEEECC
TNESLEAKLLTGNSGYDLVVPSNQFLDTQIKAGVFQKLDKSKLPNWSHQDPALLKLLDAN
CCCCCEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECC
DPGNQYGVPYMVGTVLIGFNPAKVKAALGENAPVDSWDLVFKPENMEKLKSCGVAMLDSP
CCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHCCEEEECCC
SEILPLALHYLGLDPNSQNPADYEKAKDLMLKVRPYVTYFNSAKYMTDIANGDICVAIGY
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHEEECHHEEEECCCHHEEECCCCCEEEEECC
SGSFYQFGNRAKEAGNGVVVDWRLPKEGAPIWFDTFAIPKSAKNVEEAHEFLNTLLDPKV
CCCHHHHCCHHHHCCCCEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCHHH
IASISDFLGYPNANKDSLPLINKEITGNPNLTPTSEALKKLYVVQPLPQKLERVRTRVWT
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHH
SIKSDK
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]