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The map label for this gene is gatA [H]

Identifier: 77459479

GI number: 77459479

Start: 3737776

End: 3739488

Strand: Direct

Name: gatA [H]

Synonym: Pfl01_3257

Alternate gene names: 77459479

Gene position: 3737776-3739488 (Clockwise)

Preceding gene: 77459478

Following gene: 77459481

Centisome position: 58.05

GC content: 66.02

Gene sequence:

>1713_bases
ATGATCGAAGTCACTGAAGTTTCCATTGCCCAATTGCGCGCGGCGCTCGAATCCGGCCAGACCACGGCGGTTGAACTGGT
GCAGGCTTATCTGGCGCGCATCGACGCCTATGACGGTCCGGACACCGCTACGGCCCTGAATGCGGTGATCGTGCGCAACC
CCGACGCGCTGAAAGAAGCGCAGGCCTCCGATGCCCGCCGTGCCAAAGGCGAAACCCTCGGTCCACTGGACGGCATCCCC
TACACCGCCAAAGACAGCTATCTGGTCAAAGGCCTGACCGCCGCGTCCGGCAGCCCGGCCTTCGTTGATCTGGTGGCCCA
GCGCGATGCGTTCACCATCGAGCGCCTGCGCGCGGGCGGCGCTATCTGCCTGGGCAAGACCAACATGCCGCCAATGGCCA
ATGGCGGGATGCAGCGCGGCGTCTATGGCCGGGCTGAAAGCCCGTATAACGCTGATTATCTGACTGCGCCGTTTGCTTCC
GGCTCGTCGAACGGTGCCGGCACCGCCACCGCTGCCAGTTTCTCGGCCTTCGGTCTGGCAGAAGAAACCTGGTCGAGCGG
TCGCGGCCCGGCTTCGAACAATGGCCTGTGCGCCTACACGCCGTCGCGCGGGGTGATTTCGGTACGCGGTAACTGGCCGC
TGACGCCGACCATGGACGTGGTGGTGCCCTACGCCCGTACCATGGCCGACCTGCTGGAAGTCCTCGACGTGGTGGTCGCC
GAAGACACCGACACCCGTGGCGACCTGTGGCGCCTGCAACCGTGGGTGCCGATCCCGAGCGTCGCCTCGGTGCGCCCGGC
TTCTTACCTTGAACTCGCCGTGAAAAGCGATGCACTGGCCGGCAAACGCCTCGGCGTGCCGCGCATGTACATCAACGCCG
ACCCTGAAGCCGGCACCAGCGAAGAACCGGGCATCGGCGGCCCGACCGGGCAACGCATCAACACTCGTGCCTCGGTGATC
GATCTGTGGAAACAGGCCCGTCAGGCCCTCGAAGCCGCCGGCGCCGAAGTGATCGAAGTGGATTTCCCGCTGGTCTCCAA
CTGCGAAGGTGATCGTCCGGGCGCGCCGACCGTGTTCAATCGCGGCATCGTCTCGAAAGAGTTCATGCACCACGAACTGT
GGGACCTGTCGGCCTGGGCGTTCGATGATTTCCTGCGTGCCAACGGCGACCCGAAACTCAACCGTCTGGCAGACGTCGAC
GGCCCGAAAATCTTCCCCCACGACCCGGGCACCCTGCCCAATCGTGAAGGCGACCTCGCTGCCGGCATGGACGAATACGT
GCGCATGGCCCAGCGCGGCATCACGCCGTGGGATCAGATCCCGACCCTGCCGGACGGCCTGCGCGGTCTGGAGAAGACTC
GCAAGCTCGACCTCGAAGACTGGATGGATCGTCTCGGCCTCGACGCCGTGCTGTTCCCGACCAACGCCGACGTGGGCCCC
GCCGACGCCGACGTCAACCCGGCCTCGGCGGACATCGCCTGGAGCAACGGCATCTGGGTCGCCAACGGCAACCTTGCGAT
CCGCCACCTCGGCGTGCCGACCGTCACCGTGCCGATGGGCGTGATGGCCGACATCGGCATGCCGGTGGGATTGACGTTTG
CCGGTCGCGCCTATGACGACTCGACGTTGCTGCGTCTGGCAGCGGCGTTTGAGGCGACCGGTTCCAGGCGTCTGGTGCCG
CCACGCACGCCAGCGCTGGCGGCAGGCAAGTAA

Upstream 100 bases:

>100_bases
GCCAATTTCAGGGTAGAGTTTCGCCCATCAAGAATCTTTGACATCTTTCGACGATAACAATGACCAACCACCCTTTAGCG
AACCTGTCGGCGGTAACAGC

Downstream 100 bases:

>100_bases
ATGCAAAACGGCGGGATCGATTGATCCCGCCTTTTTTTATCGCTCTGCCAACTCTTCCAGCAAATCCGCCGAAGGCACGA
AAAACAGACTGCCGGTGACC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MIEVTEVSIAQLRAALESGQTTAVELVQAYLARIDAYDGPDTATALNAVIVRNPDALKEAQASDARRAKGETLGPLDGIP
YTAKDSYLVKGLTAASGSPAFVDLVAQRDAFTIERLRAGGAICLGKTNMPPMANGGMQRGVYGRAESPYNADYLTAPFAS
GSSNGAGTATAASFSAFGLAEETWSSGRGPASNNGLCAYTPSRGVISVRGNWPLTPTMDVVVPYARTMADLLEVLDVVVA
EDTDTRGDLWRLQPWVPIPSVASVRPASYLELAVKSDALAGKRLGVPRMYINADPEAGTSEEPGIGGPTGQRINTRASVI
DLWKQARQALEAAGAEVIEVDFPLVSNCEGDRPGAPTVFNRGIVSKEFMHHELWDLSAWAFDDFLRANGDPKLNRLADVD
GPKIFPHDPGTLPNREGDLAAGMDEYVRMAQRGITPWDQIPTLPDGLRGLEKTRKLDLEDWMDRLGLDAVLFPTNADVGP
ADADVNPASADIAWSNGIWVANGNLAIRHLGVPTVTVPMGVMADIGMPVGLTFAGRAYDDSTLLRLAAAFEATGSRRLVP
PRTPALAAGK

Sequences:

>Translated_570_residues
MIEVTEVSIAQLRAALESGQTTAVELVQAYLARIDAYDGPDTATALNAVIVRNPDALKEAQASDARRAKGETLGPLDGIP
YTAKDSYLVKGLTAASGSPAFVDLVAQRDAFTIERLRAGGAICLGKTNMPPMANGGMQRGVYGRAESPYNADYLTAPFAS
GSSNGAGTATAASFSAFGLAEETWSSGRGPASNNGLCAYTPSRGVISVRGNWPLTPTMDVVVPYARTMADLLEVLDVVVA
EDTDTRGDLWRLQPWVPIPSVASVRPASYLELAVKSDALAGKRLGVPRMYINADPEAGTSEEPGIGGPTGQRINTRASVI
DLWKQARQALEAAGAEVIEVDFPLVSNCEGDRPGAPTVFNRGIVSKEFMHHELWDLSAWAFDDFLRANGDPKLNRLADVD
GPKIFPHDPGTLPNREGDLAAGMDEYVRMAQRGITPWDQIPTLPDGLRGLEKTRKLDLEDWMDRLGLDAVLFPTNADVGP
ADADVNPASADIAWSNGIWVANGNLAIRHLGVPTVTVPMGVMADIGMPVGLTFAGRAYDDSTLLRLAAAFEATGSRRLVP
PRTPALAAGK
>Mature_570_residues
MIEVTEVSIAQLRAALESGQTTAVELVQAYLARIDAYDGPDTATALNAVIVRNPDALKEAQASDARRAKGETLGPLDGIP
YTAKDSYLVKGLTAASGSPAFVDLVAQRDAFTIERLRAGGAICLGKTNMPPMANGGMQRGVYGRAESPYNADYLTAPFAS
GSSNGAGTATAASFSAFGLAEETWSSGRGPASNNGLCAYTPSRGVISVRGNWPLTPTMDVVVPYARTMADLLEVLDVVVA
EDTDTRGDLWRLQPWVPIPSVASVRPASYLELAVKSDALAGKRLGVPRMYINADPEAGTSEEPGIGGPTGQRINTRASVI
DLWKQARQALEAAGAEVIEVDFPLVSNCEGDRPGAPTVFNRGIVSKEFMHHELWDLSAWAFDDFLRANGDPKLNRLADVD
GPKIFPHDPGTLPNREGDLAAGMDEYVRMAQRGITPWDQIPTLPDGLRGLEKTRKLDLEDWMDRLGLDAVLFPTNADVGP
ADADVNPASADIAWSNGIWVANGNLAIRHLGVPTVTVPMGVMADIGMPVGLTFAGRAYDDSTLLRLAAAFEATGSRRLVP
PRTPALAAGK

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=570, Percent_Identity=25.9649122807018, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI195972892, Length=245, Percent_Identity=29.7959183673469, Blast_Score=99, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17537465, Length=256, Percent_Identity=27.734375, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=235, Percent_Identity=26.3829787234043, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=197, Percent_Identity=30.4568527918782, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648113, Length=346, Percent_Identity=27.1676300578035, Blast_Score=95, Evalue=2e-19,
Organism=Drosophila melanogaster, GI161078093, Length=258, Percent_Identity=31.3953488372093, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24644968, Length=248, Percent_Identity=29.0322580645161, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45550774, Length=222, Percent_Identity=29.7297297297297, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648435, Length=222, Percent_Identity=29.7297297297297, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648437, Length=222, Percent_Identity=29.7297297297297, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24652985, Length=248, Percent_Identity=29.0322580645161, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922090, Length=248, Percent_Identity=29.0322580645161, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24652981, Length=248, Percent_Identity=29.0322580645161, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24652983, Length=248, Percent_Identity=29.0322580645161, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356731, Length=264, Percent_Identity=26.8939393939394, Blast_Score=70, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 60505; Mature: 60505

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEVTEVSIAQLRAALESGQTTAVELVQAYLARIDAYDGPDTATALNAVIVRNPDALKEA
CEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHEEEEEECCHHHHHH
QASDARRAKGETLGPLDGIPYTAKDSYLVKGLTAASGSPAFVDLVAQRDAFTIERLRAGG
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCHHHHHHHHCCC
AICLGKTNMPPMANGGMQRGVYGRAESPYNADYLTAPFASGSSNGAGTATAASFSAFGLA
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHCCC
EETWSSGRGPASNNGLCAYTPSRGVISVRGNWPLTPTMDVVVPYARTMADLLEVLDVVVA
HHHHCCCCCCCCCCCEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
EDTDTRGDLWRLQPWVPIPSVASVRPASYLELAVKSDALAGKRLGVPRMYINADPEAGTS
CCCCCCCCEEEECCCCCCCCCCCCCCHHHHHEEHHCCCCCCCCCCCCEEEEECCCCCCCC
EEPGIGGPTGQRINTRASVIDLWKQARQALEAAGAEVIEVDFPLVSNCEGDRPGAPTVFN
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHCCCCCCCCCCCHHHH
RGIVSKEFMHHELWDLSAWAFDDFLRANGDPKLNRLADVDGPKIFPHDPGTLPNREGDLA
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCEECCCCCCCCCCCCCCHH
AGMDEYVRMAQRGITPWDQIPTLPDGLRGLEKTRKLDLEDWMDRLGLDAVLFPTNADVGP
CCHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHCCCHHHHHHHHCCCEEEECCCCCCCC
ADADVNPASADIAWSNGIWVANGNLAIRHLGVPTVTVPMGVMADIGMPVGLTFAGRAYDD
CCCCCCCCCCCEEECCCEEEECCCEEEEEECCCEEEECHHHHHHCCCCCCEEECCCCCCC
STLLRLAAAFEATGSRRLVPPRTPALAAGK
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIEVTEVSIAQLRAALESGQTTAVELVQAYLARIDAYDGPDTATALNAVIVRNPDALKEA
CEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHEEEEEECCHHHHHH
QASDARRAKGETLGPLDGIPYTAKDSYLVKGLTAASGSPAFVDLVAQRDAFTIERLRAGG
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCHHHHHHHHCCC
AICLGKTNMPPMANGGMQRGVYGRAESPYNADYLTAPFASGSSNGAGTATAASFSAFGLA
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHCCC
EETWSSGRGPASNNGLCAYTPSRGVISVRGNWPLTPTMDVVVPYARTMADLLEVLDVVVA
HHHHCCCCCCCCCCCEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
EDTDTRGDLWRLQPWVPIPSVASVRPASYLELAVKSDALAGKRLGVPRMYINADPEAGTS
CCCCCCCCEEEECCCCCCCCCCCCCCHHHHHEEHHCCCCCCCCCCCCEEEEECCCCCCCC
EEPGIGGPTGQRINTRASVIDLWKQARQALEAAGAEVIEVDFPLVSNCEGDRPGAPTVFN
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHCCCCCCCCCCCHHHH
RGIVSKEFMHHELWDLSAWAFDDFLRANGDPKLNRLADVDGPKIFPHDPGTLPNREGDLA
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCEECCCCCCCCCCCCCCHH
AGMDEYVRMAQRGITPWDQIPTLPDGLRGLEKTRKLDLEDWMDRLGLDAVLFPTNADVGP
CCHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHCCCHHHHHHHHCCCEEEECCCCCCCC
ADADVNPASADIAWSNGIWVANGNLAIRHLGVPTVTVPMGVMADIGMPVGLTFAGRAYDD
CCCCCCCCCCCEEECCCEEEECCCEEEEEECCCEEEECHHHHHHCCCCCCEEECCCCCCC
STLLRLAAAFEATGSRRLVPPRTPALAAGK
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA