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The map label for this gene is potD-A [H]

Identifier: 77459367

GI number: 77459367

Start: 3610318

End: 3611370

Strand: Direct

Name: potD-A [H]

Synonym: Pfl01_3145

Alternate gene names: 77459367

Gene position: 3610318-3611370 (Clockwise)

Preceding gene: 77459366

Following gene: 77459368

Centisome position: 56.07

GC content: 58.88

Gene sequence:

>1053_bases
ATGAAAGTGAAAAACATGCGTCTGGCAGTATCCGGTCTGGCCCTGAGTTGTTTCACCGCGTTCGGCGCCCACGCCGCCGA
GCCCAAGCAATTGTTCTTCTACAACTGGACCGATTACTACCCGGTGGACCTGCTGGCCAAGTTCGAAAAGGAGACCGGGA
TCAAGGTCACCATGGACGGCTACGACAGCAACGAAACCCTGCTCGCCAAATTGCAGGCCGGCGGCGCGGCGTATGACGTG
ATCGTGCCGTCGCAGTCGATCATGCAGACGCTGATCAAGCAGGACCTGCTGCTGGAAATCGACGCTCCGACCCTGAGCAA
CTTCCAGTACGTCAAAGGCCCGTTCCGCGATCCGAGCTTCGACCCGGGCCGCAAGTTTTCGGCGCCGTACCTGTGGGGCA
CCACCGGGTTTTCCTATGACAGCGCACGGGTACCGGGCGGCAAGCTCGATGATTCGTGGAAAGAATTCTTCGAACCGCGC
AAGGAACTGCAAGGCCAGCTCGCCGCCCTCGACACTTCCAGCAGCGTGATCAACGCCGCCAGTCATTACCTGAACGTCGA
CGAATGCACGGAAAACCCGCAGGAGGCCAAACGCATCCTCGAACTGCTGCAAAAACAGAAACCGTTTTTGAAGATGTACA
GCTCCGACAACACCGTCGACCGCATGGCCTCGGGCGAAGTGATCATGATGCAGAACTGGAACGGCTCGACGGCCCGCGCC
ACGTTGCAGAAGAGCACGATCAAATACGTGTACCCGAAAGAAGGCCTGGCGATGTTCCAGGACAACTTCGCCGTACCGAA
AAGCGCTCCGCATCCGGGCAACGCGAGGATCTTCATCGACTGGATGATGAAGCCCGAGAACGCGGCGGCCGTGTCCAACG
CGATTGCCTATGCCAACGGGATTCAGAGCGATCAGTTGCTGGATGCGAAATGGAAGGTCATGGACGCGATCAACATGCCC
GAGGAATACGCCTCGCGCCTGCGGCCGGAGAAGGAATGCAGCAACAAGGCGCGGGAGCTGCAGGATCGGATCTGGTCGAA
GCTCAAGGGTTGA

Upstream 100 bases:

>100_bases
CGCGATTTCGACCCTGATGCTGGTGATTTCCATCGTGCTGGTGGTGCTGGCCTTCTGGCTTGGCCAACGCGGCAAACAAC
AATAAACCTGGAGCGCGCTC

Downstream 100 bases:

>100_bases
TTCGGGATCGCTGGTTGATCTGAACGCCGACCACCCAGTTCGACCTGGAGAAGCCCCTCACCCTAGCCCTCTCCCAGAGG
GAGAGGGGACTGACCGCATT

Product: extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MKVKNMRLAVSGLALSCFTAFGAHAAEPKQLFFYNWTDYYPVDLLAKFEKETGIKVTMDGYDSNETLLAKLQAGGAAYDV
IVPSQSIMQTLIKQDLLLEIDAPTLSNFQYVKGPFRDPSFDPGRKFSAPYLWGTTGFSYDSARVPGGKLDDSWKEFFEPR
KELQGQLAALDTSSSVINAASHYLNVDECTENPQEAKRILELLQKQKPFLKMYSSDNTVDRMASGEVIMMQNWNGSTARA
TLQKSTIKYVYPKEGLAMFQDNFAVPKSAPHPGNARIFIDWMMKPENAAAVSNAIAYANGIQSDQLLDAKWKVMDAINMP
EEYASRLRPEKECSNKARELQDRIWSKLKG

Sequences:

>Translated_350_residues
MKVKNMRLAVSGLALSCFTAFGAHAAEPKQLFFYNWTDYYPVDLLAKFEKETGIKVTMDGYDSNETLLAKLQAGGAAYDV
IVPSQSIMQTLIKQDLLLEIDAPTLSNFQYVKGPFRDPSFDPGRKFSAPYLWGTTGFSYDSARVPGGKLDDSWKEFFEPR
KELQGQLAALDTSSSVINAASHYLNVDECTENPQEAKRILELLQKQKPFLKMYSSDNTVDRMASGEVIMMQNWNGSTARA
TLQKSTIKYVYPKEGLAMFQDNFAVPKSAPHPGNARIFIDWMMKPENAAAVSNAIAYANGIQSDQLLDAKWKVMDAINMP
EEYASRLRPEKECSNKARELQDRIWSKLKG
>Mature_350_residues
MKVKNMRLAVSGLALSCFTAFGAHAAEPKQLFFYNWTDYYPVDLLAKFEKETGIKVTMDGYDSNETLLAKLQAGGAAYDV
IVPSQSIMQTLIKQDLLLEIDAPTLSNFQYVKGPFRDPSFDPGRKFSAPYLWGTTGFSYDSARVPGGKLDDSWKEFFEPR
KELQGQLAALDTSSSVINAASHYLNVDECTENPQEAKRILELLQKQKPFLKMYSSDNTVDRMASGEVIMMQNWNGSTARA
TLQKSTIKYVYPKEGLAMFQDNFAVPKSAPHPGNARIFIDWMMKPENAAAVSNAIAYANGIQSDQLLDAKWKVMDAINMP
EEYASRLRPEKECSNKARELQDRIWSKLKG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=276, Percent_Identity=34.7826086956522, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1787078, Length=364, Percent_Identity=30.2197802197802, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1787711, Length=313, Percent_Identity=23.3226837060703, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39299; Mature: 39299

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVKNMRLAVSGLALSCFTAFGAHAAEPKQLFFYNWTDYYPVDLLAKFEKETGIKVTMDG
CCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEECC
YDSNETLLAKLQAGGAAYDVIVPSQSIMQTLIKQDLLLEIDAPTLSNFQYVKGPFRDPSF
CCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHCCEEEECCCCCCCCCEECCCCCCCCC
DPGRKFSAPYLWGTTGFSYDSARVPGGKLDDSWKEFFEPRKELQGQLAALDTSSSVINAA
CCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHEEECCCHHHHHHH
SHYLNVDECTENPQEAKRILELLQKQKPFLKMYSSDNTVDRMASGEVIMMQNWNGSTARA
HHHCCHHHHCCCHHHHHHHHHHHHCCCCCEEECCCCCHHHHHCCCCEEEEECCCCCHHHH
TLQKSTIKYVYPKEGLAMFQDNFAVPKSAPHPGNARIFIDWMMKPENAAAVSNAIAYANG
HHHHCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCC
IQSDQLLDAKWKVMDAINMPEEYASRLRPEKECSNKARELQDRIWSKLKG
CCCCCHHCHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVKNMRLAVSGLALSCFTAFGAHAAEPKQLFFYNWTDYYPVDLLAKFEKETGIKVTMDG
CCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEECC
YDSNETLLAKLQAGGAAYDVIVPSQSIMQTLIKQDLLLEIDAPTLSNFQYVKGPFRDPSF
CCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHCCEEEECCCCCCCCCEECCCCCCCCC
DPGRKFSAPYLWGTTGFSYDSARVPGGKLDDSWKEFFEPRKELQGQLAALDTSSSVINAA
CCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHEEECCCHHHHHHH
SHYLNVDECTENPQEAKRILELLQKQKPFLKMYSSDNTVDRMASGEVIMMQNWNGSTARA
HHHCCHHHHCCCHHHHHHHHHHHHCCCCCEEECCCCCHHHHHCCCCEEEEECCCCCHHHH
TLQKSTIKYVYPKEGLAMFQDNFAVPKSAPHPGNARIFIDWMMKPENAAAVSNAIAYANG
HHHHCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCC
IQSDQLLDAKWKVMDAINMPEEYASRLRPEKECSNKARELQDRIWSKLKG
CCCCCHHCHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]