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The map label for this gene is sir [H]

Identifier: 77459334

GI number: 77459334

Start: 3570761

End: 3572419

Strand: Direct

Name: sir [H]

Synonym: Pfl01_3112

Alternate gene names: 77459334

Gene position: 3570761-3572419 (Clockwise)

Preceding gene: 77459331

Following gene: 77459335

Centisome position: 55.46

GC content: 61.36

Gene sequence:

>1659_bases
ATGTACGTATACGACGAGTACGATCAGCGGATCATCGAGGACCGCGTCAAGCAGTTCCGTGATCAGACCCGACGCTATCT
GGCAGGTGAGCTGAGCGAAGAAGAATTCCGCCCCCTGCGCCTGCAAAATGGCCTGTATATCCAGCGTTTCGCGCCGATGC
TGCGCGTGGCGGTGCCTTACGGCCAGCTGACCTCGCGCCAGGTGCGCATGATGGCGAAAATCGCCCGCGACTATGACAAG
GGCTACGCCCACATCAGCACGCGGCAGAACGTGCAGTTCAACTGGCCGGCGGTGGAAGACATCCCGGACATCCTCGCGGA
ACTGGCCACCGTGCAGATGCACGCCATCCAGACCAGCGGCAACTGCCTGCGCAACGTCACCACCGACCAGTTCGCCGGTG
TCGCAGCCGACGAGTTGATCGACCCGCGTCCGTGGTGCGAAATCGTTCGTCAGTGGACGACCTTCCACCCGGAATTCGCC
TACCTGCCGCGTAAATTCAAGATCGCCGTCAACGGTTCGACCTCCGACCGTGCGGCCATCGAAGTCCACGACATCGGCCT
TGAGCCGGTCCACAACGCTGCCGGTGAACTCGGTTTCCGCGTGCTGGTCGGCGGTGGTCTGGGTCGTACTCCGGTGGTCG
GCGCGTTCATCAACGAATTCCTGCCATGGCAGGACCTGTTGAGCTACCTCGACGCGATCCTGCGGGTCTACAACCGCTAT
GGCCGTCGTGACAACAAATACAAGGCGCGGATCAAGATCCTCGTCAAAGCGCTGACTCCAGAAGTCTTCGCCCAGAAAGT
CGATGCCGAAATGGAACACCTGCGCGGCGGGCAGACCACCCTGACCGAAGCCGAAGTCCATCGCGTCGCCAAACACTTCG
TCGACCCGGACTACAAGGCGCTGAGCAATCAAGACGCCGAACTGGCCGCCCTCGACAAAGAGCATCCGGGCTTCGCCCGC
TGGCGCACGCGCAACACCCTGGCGCACAAGAAGCCGGGCTATGTGGCCGTGACCCTGTCCCTGAAGCCAACCGGCGTTGC
ACCGGGCGACATCACCGACAAGCAGCTCGACGCCGTCGCCGACTTGGCCGAGCGCTACAGCTTCGGTCAACTGCGCACGT
CCCACGAGCAGAACATCATTCTGGCCGACGTCGAGCAAAGCCAGTTGTTCACCCTGTGGGGCGAGCTGCGTGAAGCTGGC
TTCGCCACGCCGAACATCGGCCTGCTGACCGACATCATCTGCTGCCCTGGCGGTGATTTCTGCTCCCTGGCCAACGCCAA
GTCGATCCCGATCGCCGAATCGATCCAGCGCCGTTTCGACGACCTGGACTACCTGTTCGACATCGGTGAACTGGACCTGA
ACATCTCCGGCTGCATGAACGCCTGCGGTCACCACCACGTCGGCCACATCGGCATCCTCGGTGTGGACAAGAAAGGTGAA
GAGTTCTACCAGGTCTCCCTCGGTGGCAGCGCCAGCCGTGACGCCAGCCTGGGCAAGATCCTCGGCCCGTCTTTCGCTCA
GGAAGACATGCCGGACGTGATCTCCAAACTGATCGACGTGTACGTGGAACAACGCACCGAAGACGAGCGTTTCATCGACA
CCTACCAGCGTATTGGCATCGACCTCTTCAAGGAACGCGTCTATGCAGCGAATCATTAA

Upstream 100 bases:

>100_bases
GTGCGCGGCCGTGCGCGCCGAATTGCAGAGGAACCATCGGACTCCAGCAAGCGAAAACGCAGCGTTTTCCGAGTGGCCAA
AGCCTGAGAACAGGACTTAT

Downstream 100 bases:

>100_bases
GAACAACGAGGTCGTCGACGAAACCTGGCACCTGCTGCCCAAGGATTTCAACATCGACGAAATCAGCAACTGCGACGACC
TGATCGTCCCGTTGCAACTG

Product: nitrite and sulfite reductase 4Fe-4S region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MYVYDEYDQRIIEDRVKQFRDQTRRYLAGELSEEEFRPLRLQNGLYIQRFAPMLRVAVPYGQLTSRQVRMMAKIARDYDK
GYAHISTRQNVQFNWPAVEDIPDILAELATVQMHAIQTSGNCLRNVTTDQFAGVAADELIDPRPWCEIVRQWTTFHPEFA
YLPRKFKIAVNGSTSDRAAIEVHDIGLEPVHNAAGELGFRVLVGGGLGRTPVVGAFINEFLPWQDLLSYLDAILRVYNRY
GRRDNKYKARIKILVKALTPEVFAQKVDAEMEHLRGGQTTLTEAEVHRVAKHFVDPDYKALSNQDAELAALDKEHPGFAR
WRTRNTLAHKKPGYVAVTLSLKPTGVAPGDITDKQLDAVADLAERYSFGQLRTSHEQNIILADVEQSQLFTLWGELREAG
FATPNIGLLTDIICCPGGDFCSLANAKSIPIAESIQRRFDDLDYLFDIGELDLNISGCMNACGHHHVGHIGILGVDKKGE
EFYQVSLGGSASRDASLGKILGPSFAQEDMPDVISKLIDVYVEQRTEDERFIDTYQRIGIDLFKERVYAANH

Sequences:

>Translated_552_residues
MYVYDEYDQRIIEDRVKQFRDQTRRYLAGELSEEEFRPLRLQNGLYIQRFAPMLRVAVPYGQLTSRQVRMMAKIARDYDK
GYAHISTRQNVQFNWPAVEDIPDILAELATVQMHAIQTSGNCLRNVTTDQFAGVAADELIDPRPWCEIVRQWTTFHPEFA
YLPRKFKIAVNGSTSDRAAIEVHDIGLEPVHNAAGELGFRVLVGGGLGRTPVVGAFINEFLPWQDLLSYLDAILRVYNRY
GRRDNKYKARIKILVKALTPEVFAQKVDAEMEHLRGGQTTLTEAEVHRVAKHFVDPDYKALSNQDAELAALDKEHPGFAR
WRTRNTLAHKKPGYVAVTLSLKPTGVAPGDITDKQLDAVADLAERYSFGQLRTSHEQNIILADVEQSQLFTLWGELREAG
FATPNIGLLTDIICCPGGDFCSLANAKSIPIAESIQRRFDDLDYLFDIGELDLNISGCMNACGHHHVGHIGILGVDKKGE
EFYQVSLGGSASRDASLGKILGPSFAQEDMPDVISKLIDVYVEQRTEDERFIDTYQRIGIDLFKERVYAANH
>Mature_552_residues
MYVYDEYDQRIIEDRVKQFRDQTRRYLAGELSEEEFRPLRLQNGLYIQRFAPMLRVAVPYGQLTSRQVRMMAKIARDYDK
GYAHISTRQNVQFNWPAVEDIPDILAELATVQMHAIQTSGNCLRNVTTDQFAGVAADELIDPRPWCEIVRQWTTFHPEFA
YLPRKFKIAVNGSTSDRAAIEVHDIGLEPVHNAAGELGFRVLVGGGLGRTPVVGAFINEFLPWQDLLSYLDAILRVYNRY
GRRDNKYKARIKILVKALTPEVFAQKVDAEMEHLRGGQTTLTEAEVHRVAKHFVDPDYKALSNQDAELAALDKEHPGFAR
WRTRNTLAHKKPGYVAVTLSLKPTGVAPGDITDKQLDAVADLAERYSFGQLRTSHEQNIILADVEQSQLFTLWGELREAG
FATPNIGLLTDIICCPGGDFCSLANAKSIPIAESIQRRFDDLDYLFDIGELDLNISGCMNACGHHHVGHIGILGVDKKGE
EFYQVSLGGSASRDASLGKILGPSFAQEDMPDVISKLIDVYVEQRTEDERFIDTYQRIGIDLFKERVYAANH

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

None

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 62263; Mature: 62263

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVYDEYDQRIIEDRVKQFRDQTRRYLAGELSEEEFRPLRLQNGLYIQRFAPMLRVAVPY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHCCCEEHHHHHHHHHHHCCC
GQLTSRQVRMMAKIARDYDKGYAHISTRQNVQFNWPAVEDIPDILAELATVQMHAIQTSG
CHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHEECCC
NCLRNVTTDQFAGVAADELIDPRPWCEIVRQWTTFHPEFAYLPRKFKIAVNGSTSDRAAI
CHHHCCCHHHHCCCCHHHHCCCCHHHHHHHHHHCCCCCHHHCCCEEEEEECCCCCCCEEE
EVHDIGLEPVHNAAGELGFRVLVGGGLGRTPVVGAFINEFLPWQDLLSYLDAILRVYNRY
EEEECCCCHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
GRRDNKYKARIKILVKALTPEVFAQKVDAEMEHLRGGQTTLTEAEVHRVAKHFVDPDYKA
CCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHH
LSNQDAELAALDKEHPGFARWRTRNTLAHKKPGYVAVTLSLKPTGVAPGDITDKQLDAVA
HCCCCCCEEECCCCCCCCHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHH
DLAERYSFGQLRTSHEQNIILADVEQSQLFTLWGELREAGFATPNIGLLTDIICCPGGDF
HHHHHHCHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCH
CSLANAKSIPIAESIQRRFDDLDYLFDIGELDLNISGCMNACGHHHVGHIGILGVDKKGE
HCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHCCCCCCCEEEEEECCCCCC
EFYQVSLGGSASRDASLGKILGPSFAQEDMPDVISKLIDVYVEQRTEDERFIDTYQRIGI
EEEEEECCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
DLFKERVYAANH
HHHHHHHHHCCC
>Mature Secondary Structure
MYVYDEYDQRIIEDRVKQFRDQTRRYLAGELSEEEFRPLRLQNGLYIQRFAPMLRVAVPY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHCCCEEHHHHHHHHHHHCCC
GQLTSRQVRMMAKIARDYDKGYAHISTRQNVQFNWPAVEDIPDILAELATVQMHAIQTSG
CHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHEECCC
NCLRNVTTDQFAGVAADELIDPRPWCEIVRQWTTFHPEFAYLPRKFKIAVNGSTSDRAAI
CHHHCCCHHHHCCCCHHHHCCCCHHHHHHHHHHCCCCCHHHCCCEEEEEECCCCCCCEEE
EVHDIGLEPVHNAAGELGFRVLVGGGLGRTPVVGAFINEFLPWQDLLSYLDAILRVYNRY
EEEECCCCHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
GRRDNKYKARIKILVKALTPEVFAQKVDAEMEHLRGGQTTLTEAEVHRVAKHFVDPDYKA
CCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHH
LSNQDAELAALDKEHPGFARWRTRNTLAHKKPGYVAVTLSLKPTGVAPGDITDKQLDAVA
HCCCCCCEEECCCCCCCCHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHH
DLAERYSFGQLRTSHEQNIILADVEQSQLFTLWGELREAGFATPNIGLLTDIICCPGGDF
HHHHHHCHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCH
CSLANAKSIPIAESIQRRFDDLDYLFDIGELDLNISGCMNACGHHHVGHIGILGVDKKGE
HCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHCCCCCCCEEEEEECCCCCC
EFYQVSLGGSASRDASLGKILGPSFAQEDMPDVISKLIDVYVEQRTEDERFIDTYQRIGI
EEEEEECCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
DLFKERVYAANH
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]