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The map label for this gene is pspF [C]

Identifier: 77459319

GI number: 77459319

Start: 3546478

End: 3547479

Strand: Reverse

Name: pspF [C]

Synonym: Pfl01_3097

Alternate gene names: 77459319

Gene position: 3547479-3546478 (Counterclockwise)

Preceding gene: 77459320

Following gene: 77459318

Centisome position: 55.1

GC content: 62.77

Gene sequence:

>1002_bases
ATGACCGACACCCCGCATTTCCCGCTCTCCGCCGTGGTCGGCGCCGATGATTTGAAACTGGCGCTGTACCTGACGGCCAT
TGACCCGAAAATCGGCGGCGTGCTGATCGAAGGCCCGCGTGGCATGGCCAAGTCGACGCTCGCCCGGGGGCTGGCGGATC
TGTTGGCCAGTGGTCAGTTCGTTACCTTGCCGTTGGGTGCTACTGAAGAGCGATTGGTCGGCACGCTGGATCTGGACGCG
GCGTTGAGCGACGGTCGTGCGCAGTTTTCTCCGGGTGTGCTGGCGAAGGCGGACGGCGGCGTGTTGTACGTCGATGAGGT
GAATCTGTTGCCGGATCATCTGGTGGATCTGCTGCTTGATGTGGCTGCCAGCGGCACCAACCTGATCGAGCGCGACGGCA
TTTCCCATCGACATTCGGCGAAGTTCGTATTGATCGGCACGATGAACCCGGAAGAGGGGGAATTGCGTCCGCAATTGCTG
GATCGTTTCGGCCTTAACGTCGCGCTGAGCGGTCATACGGCGCCGGCCGAGCGGGGGCAGATCATTCGTCGTCGGCTGGA
TTTCGACAGCGACCCGCAGGTGTTTTGTGCGCAGTGGGAGGCTGATCAGCAAGCTCTGCGTGAGCGTTGTGAAAGCGCCC
GCCATGCCTTGGCGAATATTCCCCTCGACGATGAAGCGCTGGCACAGATCACCGAGCGCTGCTTTGCGGCAGGCGTCGAC
GGTTTGCGCGCCGATCTGGTCTGGCTGCGTGCGGCGCGGGCTCATGCGGCGTGGCGCGGGGCTGAAACCATTGCCGAGCA
AGACATTGATGCCGTGGCTGAGCTTGCATTGCGCCATCGTCGCCGCGAACAGTCTTCATCCCCTGCACAGCCACCGGCGC
AATCGCCGTCCGGTACTCACGCCGAACCGAGCGAAGGGCAGGGGCAATGGGGTGAAATGCCGGCACCGGCATTGGTTACC
GGCGCACGCCGTGAAGTTCCGACCTGGCCAAAAAAGCTTTAG

Upstream 100 bases:

>100_bases
TCGAAGCGCAGCAGCGCGGGATGTGGCAGGAGCCGGGCGCCTACAAAGAAGCCCTGGAAAACCTGCTGCTGGACATAGAA
GAAGAGAGCTGAGACTGAAC

Downstream 100 bases:

>100_bases
GCATTCGCCCCCGGTCCGACGCGGGGGCGAATGCCAGACCCCGCGCCGGACGCCTCGATAACGGTCGCCAGGGCACGCGT
CACGAGGCTTGCAGTGGCTC

Product: protoporphyrin IX magnesium-chelatase

Products: ADP; phosphate; Mg-protoporphyrin IX; H+

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MTDTPHFPLSAVVGADDLKLALYLTAIDPKIGGVLIEGPRGMAKSTLARGLADLLASGQFVTLPLGATEERLVGTLDLDA
ALSDGRAQFSPGVLAKADGGVLYVDEVNLLPDHLVDLLLDVAASGTNLIERDGISHRHSAKFVLIGTMNPEEGELRPQLL
DRFGLNVALSGHTAPAERGQIIRRRLDFDSDPQVFCAQWEADQQALRERCESARHALANIPLDDEALAQITERCFAAGVD
GLRADLVWLRAARAHAAWRGAETIAEQDIDAVAELALRHRRREQSSSPAQPPAQSPSGTHAEPSEGQGQWGEMPAPALVT
GARREVPTWPKKL

Sequences:

>Translated_333_residues
MTDTPHFPLSAVVGADDLKLALYLTAIDPKIGGVLIEGPRGMAKSTLARGLADLLASGQFVTLPLGATEERLVGTLDLDA
ALSDGRAQFSPGVLAKADGGVLYVDEVNLLPDHLVDLLLDVAASGTNLIERDGISHRHSAKFVLIGTMNPEEGELRPQLL
DRFGLNVALSGHTAPAERGQIIRRRLDFDSDPQVFCAQWEADQQALRERCESARHALANIPLDDEALAQITERCFAAGVD
GLRADLVWLRAARAHAAWRGAETIAEQDIDAVAELALRHRRREQSSSPAQPPAQSPSGTHAEPSEGQGQWGEMPAPALVT
GARREVPTWPKKL
>Mature_332_residues
TDTPHFPLSAVVGADDLKLALYLTAIDPKIGGVLIEGPRGMAKSTLARGLADLLASGQFVTLPLGATEERLVGTLDLDAA
LSDGRAQFSPGVLAKADGGVLYVDEVNLLPDHLVDLLLDVAASGTNLIERDGISHRHSAKFVLIGTMNPEEGELRPQLLD
RFGLNVALSGHTAPAERGQIIRRRLDFDSDPQVFCAQWEADQQALRERCESARHALANIPLDDEALAQITERCFAAGVDG
LRADLVWLRAARAHAAWRGAETIAEQDIDAVAELALRHRRREQSSSPAQPPAQSPSGTHAEPSEGQGQWGEMPAPALVTG
ARREVPTWPKKL

Specific function: Transcriptional Activator For The Phage Shock Protein (Psp) Operon (Pspabce). [C]

COG id: COG1239

COG function: function code H; Mg-chelatase subunit ChlI

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 VWFA domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011704
- InterPro:   IPR012804
- InterPro:   IPR002035 [H]

Pfam domain/function: PF07728 AAA_5; PF00092 VWA [H]

EC number: 6.6.1.1

Molecular weight: Translated: 35772; Mature: 35641

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTPHFPLSAVVGADDLKLALYLTAIDPKIGGVLIEGPRGMAKSTLARGLADLLASGQF
CCCCCCCCHHEECCCCCEEEEEEEEEECCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCE
VTLPLGATEERLVGTLDLDAALSDGRAQFSPGVLAKADGGVLYVDEVNLLPDHLVDLLLD
EEEECCCCHHHHEEEECHHHHHCCCCCCCCCCEEEECCCCEEEEECCCCCHHHHHHHHHH
VAASGTNLIERDGISHRHSAKFVLIGTMNPEEGELRPQLLDRFGLNVALSGHTAPAERGQ
HHCCCCCHHHHCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCEEEECCCCCCHHHHH
IIRRRLDFDSDPQVFCAQWEADQQALRERCESARHALANIPLDDEALAQITERCFAAGVD
HHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
GLRADLVWLRAARAHAAWRGAETIAEQDIDAVAELALRHRRREQSSSPAQPPAQSPSGTH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
AEPSEGQGQWGEMPAPALVTGARREVPTWPKKL
CCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDTPHFPLSAVVGADDLKLALYLTAIDPKIGGVLIEGPRGMAKSTLARGLADLLASGQF
CCCCCCCHHEECCCCCEEEEEEEEEECCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCE
VTLPLGATEERLVGTLDLDAALSDGRAQFSPGVLAKADGGVLYVDEVNLLPDHLVDLLLD
EEEECCCCHHHHEEEECHHHHHCCCCCCCCCCEEEECCCCEEEEECCCCCHHHHHHHHHH
VAASGTNLIERDGISHRHSAKFVLIGTMNPEEGELRPQLLDRFGLNVALSGHTAPAERGQ
HHCCCCCHHHHCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCEEEECCCCCCHHHHH
IIRRRLDFDSDPQVFCAQWEADQQALRERCESARHALANIPLDDEALAQITERCFAAGVD
HHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
GLRADLVWLRAARAHAAWRGAETIAEQDIDAVAELALRHRRREQSSSPAQPPAQSPSGTH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
AEPSEGQGQWGEMPAPALVTGARREVPTWPKKL
CCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; protoporphyrin IX; Mg2+; H2O

Specific reaction: ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]