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The map label for this gene is intB [H]

Identifier: 77459286

GI number: 77459286

Start: 3504962

End: 3506278

Strand: Reverse

Name: intB [H]

Synonym: Pfl01_3064

Alternate gene names: 77459286

Gene position: 3506278-3504962 (Counterclockwise)

Preceding gene: 77459287

Following gene: 77459285

Centisome position: 54.46

GC content: 53.83

Gene sequence:

>1317_bases
ATGTCGCGCACAACTGCTCCGCTTACCGACGCTGCTTGCCGTTTAGCAAGGCCAACGGACCGTCCCTACAAGCTTTTCGA
CGGCGACGGTCTCTATCTCCTAATTCAACCCAATGGCCGTAAAGGCTGGCGTCTCAGGTATGTAAAGCCTGACGGCCGGG
AGGGGTTGACCTCCCTCGGCAGCTATCCCGTGATTGGCCTGGCCGAAGCTCGCCAGAAGCGTTTAGATGCGAAGCGAATG
CTCGCAGACGGCATTGATCCCGTCGAGAGCAAGCGTAAAGCCAAAACGGAAGATGCTGTTAACGGCCGTACTTTTGAAAG
CGTTGCGTTGGACTGGCACAAGAACATGTCGGCCAGATGGACACTCGACCATTCCAAAAATGTGTTCAACCGTCTGAATA
ACTATGTGTTCCCGTTTATCGGTGCTCGCGCGATTGCTGATCTCGATACTTATGATCTGATGCAACCTTTGGAGGCGATC
AGAAAACGAGGAACAATTGACGTCGCCTTAAGGGTACAAAGATACCTGCAAAGTATTATGCGTGAGGCAAAACGCCTTCG
TCTTATTCCGATGAACCCTGCGTACGATCTTCAGGGTTTGATCAAAGCGCCGCGAGTCTCCCATCGGCCTACATTGCCCC
TGTCACGCCTGCCTGAATTGCTGGCACGAATCTATGCCTACAAGGGCCGAGAGCTGACCCGGCTAACGCTCATGCTCTCG
CTGCACGTGTTTGTTCGCTCGAGTGAGTTACGTTTCGCTCGTTGGTGTGAGTTCGACCTCAAGCGTTGCGTATGGGAGAT
CCCGGATACTCGGCCGGAGCTGGAGGACGTACGCCATTCAACACGGGGGACGAAAATGGCGGGCGATGTTCATCTTGTGC
CTTTATCGCCGCAAGTTGTGGCCGTGCTTGAACAGATCCACACCCTCACTGGCGCATTCGACCTGGTGTTTGCCGGTGAT
GCTAGGGCCTGGAAGCCGATGTCAGAAAACACCGTGAACAAAGCGTTGCGCACCATGGGATACGACACGAAGACAGAGAT
TTGTGGTCACGGATTTCGCTCAATGGCCTGTAGCGCACTGATCGAGTCAGGTCTGTGGTCTGAGACGGCCATCGAAAGGC
AAATGAGTCATAGAGAGCGCAACAGCGTTCGAGCTGCCTACATTCACAAGGCAGAGTTCATTGAGGAGCGTCGGTTGATC
ATGAACTGGTGGAGCCGGTACCTTGCGGCAAATCTTCAGGAGCATGTCACGCCTCATGAGTTTGCGAATCAGGTCGGCGA
GAATGTCACTCGATTAAAAGTAAACGTGGCGCAATGA

Upstream 100 bases:

>100_bases
TTTTAGCATTCCGTCGGATTCTCGTCCATTCTTCGGGAATCCAGTCATTTAGAGGGTAGAGTTAGGGGTAGTCTTTGGTT
CGATTAAGGATGATACCCTT

Downstream 100 bases:

>100_bases
AGTAAGCGTAGGGGTAGTGGTTCGTTAGGGTGGCGACTAAAAGATCGTTCGCCAATTGCTGTCCTCACTCCGGATAATTT
CGACGTGACCACGCGGATCG

Product: Phage integrase

Products: NA

Alternate protein names: Int(P4) [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MSRTTAPLTDAACRLARPTDRPYKLFDGDGLYLLIQPNGRKGWRLRYVKPDGREGLTSLGSYPVIGLAEARQKRLDAKRM
LADGIDPVESKRKAKTEDAVNGRTFESVALDWHKNMSARWTLDHSKNVFNRLNNYVFPFIGARAIADLDTYDLMQPLEAI
RKRGTIDVALRVQRYLQSIMREAKRLRLIPMNPAYDLQGLIKAPRVSHRPTLPLSRLPELLARIYAYKGRELTRLTLMLS
LHVFVRSSELRFARWCEFDLKRCVWEIPDTRPELEDVRHSTRGTKMAGDVHLVPLSPQVVAVLEQIHTLTGAFDLVFAGD
ARAWKPMSENTVNKALRTMGYDTKTEICGHGFRSMACSALIESGLWSETAIERQMSHRERNSVRAAYIHKAEFIEERRLI
MNWWSRYLAANLQEHVTPHEFANQVGENVTRLKVNVAQ

Sequences:

>Translated_438_residues
MSRTTAPLTDAACRLARPTDRPYKLFDGDGLYLLIQPNGRKGWRLRYVKPDGREGLTSLGSYPVIGLAEARQKRLDAKRM
LADGIDPVESKRKAKTEDAVNGRTFESVALDWHKNMSARWTLDHSKNVFNRLNNYVFPFIGARAIADLDTYDLMQPLEAI
RKRGTIDVALRVQRYLQSIMREAKRLRLIPMNPAYDLQGLIKAPRVSHRPTLPLSRLPELLARIYAYKGRELTRLTLMLS
LHVFVRSSELRFARWCEFDLKRCVWEIPDTRPELEDVRHSTRGTKMAGDVHLVPLSPQVVAVLEQIHTLTGAFDLVFAGD
ARAWKPMSENTVNKALRTMGYDTKTEICGHGFRSMACSALIESGLWSETAIERQMSHRERNSVRAAYIHKAEFIEERRLI
MNWWSRYLAANLQEHVTPHEFANQVGENVTRLKVNVAQ
>Mature_437_residues
SRTTAPLTDAACRLARPTDRPYKLFDGDGLYLLIQPNGRKGWRLRYVKPDGREGLTSLGSYPVIGLAEARQKRLDAKRML
ADGIDPVESKRKAKTEDAVNGRTFESVALDWHKNMSARWTLDHSKNVFNRLNNYVFPFIGARAIADLDTYDLMQPLEAIR
KRGTIDVALRVQRYLQSIMREAKRLRLIPMNPAYDLQGLIKAPRVSHRPTLPLSRLPELLARIYAYKGRELTRLTLMLSL
HVFVRSSELRFARWCEFDLKRCVWEIPDTRPELEDVRHSTRGTKMAGDVHLVPLSPQVVAVLEQIHTLTGAFDLVFAGDA
RAWKPMSENTVNKALRTMGYDTKTEICGHGFRSMACSALIESGLWSETAIERQMSHRERNSVRAAYIHKAEFIEERRLIM
NWWSRYLAANLQEHVTPHEFANQVGENVTRLKVNVAQ

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=412, Percent_Identity=31.5533980582524, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI145693166, Length=426, Percent_Identity=33.8028169014084, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1788690, Length=407, Percent_Identity=29.2383292383292, Blast_Score=199, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 50222; Mature: 50090

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTTAPLTDAACRLARPTDRPYKLFDGDGLYLLIQPNGRKGWRLRYVKPDGREGLTSLG
CCCCCCCHHHHHHHHCCCCCCCCEEECCCCEEEEEECCCCCCCEEEEECCCCHHHHHHCC
SYPVIGLAEARQKRLDAKRMLADGIDPVESKRKAKTEDAVNGRTFESVALDWHKNMSARW
CCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHCCCCCHHHHHHHHHCCCCEEE
TLDHSKNVFNRLNNYVFPFIGARAIADLDTYDLMQPLEAIRKRGTIDVALRVQRYLQSIM
ECCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
REAKRLRLIPMNPAYDLQGLIKAPRVSHRPTLPLSRLPELLARIYAYKGRELTRLTLMLS
HHHHHEEEEECCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
LHVFVRSSELRFARWCEFDLKRCVWEIPDTRPELEDVRHSTRGTKMAGDVHLVPLSPQVV
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEECCCEEEEECCHHHH
AVLEQIHTLTGAFDLVFAGDARAWKPMSENTVNKALRTMGYDTKTEICGHGFRSMACSAL
HHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHH
IESGLWSETAIERQMSHRERNSVRAAYIHKAEFIEERRLIMNWWSRYLAANLQEHVTPHE
HHCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
FANQVGENVTRLKVNVAQ
HHHHHCCCEEEEEEEECC
>Mature Secondary Structure 
SRTTAPLTDAACRLARPTDRPYKLFDGDGLYLLIQPNGRKGWRLRYVKPDGREGLTSLG
CCCCCCHHHHHHHHCCCCCCCCEEECCCCEEEEEECCCCCCCEEEEECCCCHHHHHHCC
SYPVIGLAEARQKRLDAKRMLADGIDPVESKRKAKTEDAVNGRTFESVALDWHKNMSARW
CCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHCCCCCHHHHHHHHHCCCCEEE
TLDHSKNVFNRLNNYVFPFIGARAIADLDTYDLMQPLEAIRKRGTIDVALRVQRYLQSIM
ECCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
REAKRLRLIPMNPAYDLQGLIKAPRVSHRPTLPLSRLPELLARIYAYKGRELTRLTLMLS
HHHHHEEEEECCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
LHVFVRSSELRFARWCEFDLKRCVWEIPDTRPELEDVRHSTRGTKMAGDVHLVPLSPQVV
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEECCCEEEEECCHHHH
AVLEQIHTLTGAFDLVFAGDARAWKPMSENTVNKALRTMGYDTKTEICGHGFRSMACSAL
HHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHH
IESGLWSETAIERQMSHRERNSVRAAYIHKAEFIEERRLIMNWWSRYLAANLQEHVTPHE
HHCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
FANQVGENVTRLKVNVAQ
HHHHHCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]