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The map label for this gene is ecpD [H]

Identifier: 77459265

GI number: 77459265

Start: 3483713

End: 3484462

Strand: Direct

Name: ecpD [H]

Synonym: Pfl01_3043

Alternate gene names: 77459265

Gene position: 3483713-3484462 (Clockwise)

Preceding gene: 77459264

Following gene: 77459266

Centisome position: 54.11

GC content: 57.87

Gene sequence:

>750_bases
ATGTTCTTACGGCATTTCTTATCCCTGTGCGTCGGCCTCTTGGGTCTGTTCGCCGTTAATCAGGCCATGGCCGGTATTTC
GTTGAGCAGCACCCGGCTGATTTTCGATGGCAAGCATAAAGAAGCCAGCATCACCGTGCGCAATAGCGGTGCAGACGTAT
TGGTTCAGTCCTGGGTCGACACAGACAGCGACGAAGCAGGTTCAGCGCCCTTCGCCGTCACCCCGCCTCTGGTGCGGGTC
AGCGGCGATCAACAGCAGATTCTGCGGGTTATCTACGAAGGCACCGGTATGCCGAGCGACCGCGAGTCGGTGGTGTGGCT
GAATGTGCAGGAAATCCCCCAATCGGCGAAAACCGCGAACACCCTGCAACTGGCGGTACGTCAGCGGATCAAGGTGTTCT
TTCGCCCCGCCGGTTTGCCAAACAATGCCTATCAAGCGCCAACCGAGCTGCGCTGGCGACTGATCGAGCGCGCCGGCAAG
AGCGTGCTGGTGGTGAACAATCCAGGCCTCTATCACGTATCGATTGCCGACATAACGATGCAAGCGGGCGCCAGCAGCGA
GAAGCCATTCGATTCGATGATGATTGCGCCCGGCGAACACAAAGAATTCGTGCTCAAACAAACTCACGCGGCCAGTTCTG
CGCGACTGTCATTCAGCAGCATCAACGACTACGGCGCCCGAGACCGTTATGTCGCGCAACTTTCCGGCAGCGCCGAAGTT
AGCGCCAGCCTGGATAAAGAATCGCCATAA

Upstream 100 bases:

>100_bases
CCTGCCGTTCACCCTGCAATACGAATAATCGCGCGATTCATCGAGCCAGTGCAGGGCGCAACGGCCCTGCACTTCCCTTC
ACTTTGCGCGGTAATTGATC

Downstream 100 bases:

>100_bases
CTCGCGTGCCGCTTTATCAGCCGCACTTTCACTGTATTTCTCTTCGCTCCGTTAGTTCGTATCGGAGGTGATATAGGGTT
CCTGCATGTTTTTTTTAAAG

Product: pili assembly chaperone

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MFLRHFLSLCVGLLGLFAVNQAMAGISLSSTRLIFDGKHKEASITVRNSGADVLVQSWVDTDSDEAGSAPFAVTPPLVRV
SGDQQQILRVIYEGTGMPSDRESVVWLNVQEIPQSAKTANTLQLAVRQRIKVFFRPAGLPNNAYQAPTELRWRLIERAGK
SVLVVNNPGLYHVSIADITMQAGASSEKPFDSMMIAPGEHKEFVLKQTHAASSARLSFSSINDYGARDRYVAQLSGSAEV
SASLDKESP

Sequences:

>Translated_249_residues
MFLRHFLSLCVGLLGLFAVNQAMAGISLSSTRLIFDGKHKEASITVRNSGADVLVQSWVDTDSDEAGSAPFAVTPPLVRV
SGDQQQILRVIYEGTGMPSDRESVVWLNVQEIPQSAKTANTLQLAVRQRIKVFFRPAGLPNNAYQAPTELRWRLIERAGK
SVLVVNNPGLYHVSIADITMQAGASSEKPFDSMMIAPGEHKEFVLKQTHAASSARLSFSSINDYGARDRYVAQLSGSAEV
SASLDKESP
>Mature_249_residues
MFLRHFLSLCVGLLGLFAVNQAMAGISLSSTRLIFDGKHKEASITVRNSGADVLVQSWVDTDSDEAGSAPFAVTPPLVRV
SGDQQQILRVIYEGTGMPSDRESVVWLNVQEIPQSAKTANTLQLAVRQRIKVFFRPAGLPNNAYQAPTELRWRLIERAGK
SVLVVNNPGLYHVSIADITMQAGASSEKPFDSMMIAPGEHKEFVLKQTHAASSARLSFSSINDYGARDRYVAQLSGSAEV
SASLDKESP

Specific function: Probably mediates assembly of a pilin [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1786333, Length=240, Percent_Identity=37.9166666666667, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1789532, Length=231, Percent_Identity=35.4978354978355, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787171, Length=207, Percent_Identity=33.3333333333333, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1790771, Length=229, Percent_Identity=33.6244541484716, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1786743, Length=227, Percent_Identity=32.15859030837, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788677, Length=226, Percent_Identity=31.4159292035398, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1788428, Length=229, Percent_Identity=30.1310043668122, Blast_Score=97, Evalue=7e-22,
Organism=Escherichia coli, GI1786936, Length=230, Percent_Identity=26.9565217391304, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87082203, Length=214, Percent_Identity=27.1028037383178, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI87081806, Length=226, Percent_Identity=24.3362831858407, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 27077; Mature: 27077

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLRHFLSLCVGLLGLFAVNQAMAGISLSSTRLIFDGKHKEASITVRNSGADVLVQSWVD
CHHHHHHHHHHHHHHHHHHHHHHHCCEECCEEEEEECCCCCEEEEEECCCCCEEEEECCC
TDSDEAGSAPFAVTPPLVRVSGDQQQILRVIYEGTGMPSDRESVVWLNVQEIPQSAKTAN
CCCCCCCCCCEEECCCEEEECCCHHHHHHHHHHCCCCCCCCCCEEEEEHHHCCCHHHHHH
TLQLAVRQRIKVFFRPAGLPNNAYQAPTELRWRLIERAGKSVLVVNNPGLYHVSIADITM
HHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCEEEEEEEEEEE
QAGASSEKPFDSMMIAPGEHKEFVLKQTHAASSARLSFSSINDYGARDRYVAQLSGSAEV
ECCCCCCCCCCCEEECCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEECCCCEE
SASLDKESP
EECCCCCCC
>Mature Secondary Structure
MFLRHFLSLCVGLLGLFAVNQAMAGISLSSTRLIFDGKHKEASITVRNSGADVLVQSWVD
CHHHHHHHHHHHHHHHHHHHHHHHCCEECCEEEEEECCCCCEEEEEECCCCCEEEEECCC
TDSDEAGSAPFAVTPPLVRVSGDQQQILRVIYEGTGMPSDRESVVWLNVQEIPQSAKTAN
CCCCCCCCCCEEECCCEEEECCCHHHHHHHHHHCCCCCCCCCCEEEEEHHHCCCHHHHHH
TLQLAVRQRIKVFFRPAGLPNNAYQAPTELRWRLIERAGKSVLVVNNPGLYHVSIADITM
HHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCEEEEEEEEEEE
QAGASSEKPFDSMMIAPGEHKEFVLKQTHAASSARLSFSSINDYGARDRYVAQLSGSAEV
ECCCCCCCCCCCEEECCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEECCCCEE
SASLDKESP
EECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8102362; 8202364; 9278503 [H]