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The map label for this gene is traC [H]

Identifier: 77459255

GI number: 77459255

Start: 3469935

End: 3470897

Strand: Direct

Name: traC [H]

Synonym: Pfl01_3033

Alternate gene names: 77459255

Gene position: 3469935-3470897 (Clockwise)

Preceding gene: 77459254

Following gene: 77459256

Centisome position: 53.89

GC content: 56.49

Gene sequence:

>963_bases
ATGCGCGATATCTACCAAGACGTGACTGACAAGATCGTTACCGCTTTGGAACAAGGCGTAGCCCCCTGGATCAAACCCTG
GTCCTCAAGTGGCTCAGCTCACGTCGGTCATCATCACCCCTACCCCATTAACGCCATTACGCGGCGTCCCTATTCGGGCA
TCAATTTACCGCTGCTCTGGGCCGAGGCCAGGTTGCAGGGGTTCACTCAAGATCGTTGGCTGACCTTCAACCAGGCCAAA
AAAGCCGGTGGGCACATCCGCAAGGGTGAGCACAGCACTCTGGCGGTGCTCTACAAGCCGATGGAGCGCGAGGAACAGAC
CGAGTCAGGTCAACCCGTACTTGATGAGAACGGTCAGCCCAAGGTCGCTCATTTCGGGATTCTCAAGACGCATTTTCTGT
TCAACATCGAGCAAACGGAGGGGCTCGAAACGCTCAACGAAACCGTGCTCGAGACGGAGCCGAGCGATCCCTTCCTCCCC
AACGGCGCTGCGGAAAATCTGTTGTTACGTTCGGGCGCAAACATCATTCATCGATTTGCCGACGAAGCTTTTTACCACCC
GATCAGGGATCTCATCCAACTGCCGACAAAAGCGCAATTTCACGATCAAGGTGGGTACTACGCCACCGCACTTCACGAGT
TGACGCACTGGACCGGGCATCACACTCGGTTGCAGCGCGAAGGCGTGACGTCATCCTGTCCGTTCGGCTCGCCAGGCTAT
GCCTTTGAGGAGTTGATCGCCGAGATGGGCGCTGCGTTTTTATGTGCTTACGCCGGCATTCAGGGAGAGCTGAGGCATGG
GGGCTATATCGATTCCTGGCTCGGCCTGCTCAAGGCTGACAAACGTGCAATCTTCCGCGCCAGTGGCCAGGCCCGAAGCG
CCTCAGAGTATGTACTCAACCTGGAGCAGCAACTGAGGCAAGATGTCTTGGATGACTCGCTGTGCATGAACGATGGAGCT
TAA

Upstream 100 bases:

>100_bases
CGGACAGTATGCAAGTCCTGCATCAATCCAACCCATCACTTACCGGGGAACCCTCCCCGACGGGCAAGGCGTTCCCCCGT
TTTCTCTCGAGGAAAATGCC

Downstream 100 bases:

>100_bases
TCGTTACCTCACCGCTGCAAACGCTTCGCAGGGCCCAACGCCAGTTGAGCCCTTTTTTCTGCTCCCAGAAAACAGACGGA
ATCATACGGCGGGCCCTCAC

Product: DNA primase

Products: NA

Alternate protein names: Replication primase [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MRDIYQDVTDKIVTALEQGVAPWIKPWSSSGSAHVGHHHPYPINAITRRPYSGINLPLLWAEARLQGFTQDRWLTFNQAK
KAGGHIRKGEHSTLAVLYKPMEREEQTESGQPVLDENGQPKVAHFGILKTHFLFNIEQTEGLETLNETVLETEPSDPFLP
NGAAENLLLRSGANIIHRFADEAFYHPIRDLIQLPTKAQFHDQGGYYATALHELTHWTGHHTRLQREGVTSSCPFGSPGY
AFEELIAEMGAAFLCAYAGIQGELRHGGYIDSWLGLLKADKRAIFRASGQARSASEYVLNLEQQLRQDVLDDSLCMNDGA

Sequences:

>Translated_320_residues
MRDIYQDVTDKIVTALEQGVAPWIKPWSSSGSAHVGHHHPYPINAITRRPYSGINLPLLWAEARLQGFTQDRWLTFNQAK
KAGGHIRKGEHSTLAVLYKPMEREEQTESGQPVLDENGQPKVAHFGILKTHFLFNIEQTEGLETLNETVLETEPSDPFLP
NGAAENLLLRSGANIIHRFADEAFYHPIRDLIQLPTKAQFHDQGGYYATALHELTHWTGHHTRLQREGVTSSCPFGSPGY
AFEELIAEMGAAFLCAYAGIQGELRHGGYIDSWLGLLKADKRAIFRASGQARSASEYVLNLEQQLRQDVLDDSLCMNDGA
>Mature_320_residues
MRDIYQDVTDKIVTALEQGVAPWIKPWSSSGSAHVGHHHPYPINAITRRPYSGINLPLLWAEARLQGFTQDRWLTFNQAK
KAGGHIRKGEHSTLAVLYKPMEREEQTESGQPVLDENGQPKVAHFGILKTHFLFNIEQTEGLETLNETVLETEPSDPFLP
NGAAENLLLRSGANIIHRFADEAFYHPIRDLIQLPTKAQFHDQGGYYATALHELTHWTGHHTRLQREGVTSSCPFGSPGY
AFEELIAEMGAAFLCAYAGIQGELRHGGYIDSWLGLLKADKRAIFRASGQARSASEYVLNLEQQLRQDVLDDSLCMNDGA

Specific function: Required for autonomous replication in E.coli. Transferred into the recipient cell during bacterial conjugation. Catalyzes the synthesis of short oligoribonucleotide primers with CpA or pCpA at their 5'-termini on a single stranded template DNA [H]

COG id: COG4227

COG function: function code L; Antirestriction protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013610
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08401 DUF1738; PF01751 Toprim [H]

EC number: NA

Molecular weight: Translated: 35770; Mature: 35770

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDIYQDVTDKIVTALEQGVAPWIKPWSSSGSAHVGHHHPYPINAITRRPYSGINLPLLW
CCHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEE
AEARLQGFTQDRWLTFNQAKKAGGHIRKGEHSTLAVLYKPMEREEQTESGQPVLDENGQP
HHHHHCCCCCCCEEEHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHCCCCCCCCCCCCC
KVAHFGILKTHFLFNIEQTEGLETLNETVLETEPSDPFLPNGAAENLLLRSGANIIHRFA
CHHHHHHHHHHHEEECHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHH
DEAFYHPIRDLIQLPTKAQFHDQGGYYATALHELTHWTGHHTRLQREGVTSSCPFGSPGY
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCH
AFEELIAEMGAAFLCAYAGIQGELRHGGYIDSWLGLLKADKRAIFRASGQARSASEYVLN
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LEQQLRQDVLDDSLCMNDGA
HHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRDIYQDVTDKIVTALEQGVAPWIKPWSSSGSAHVGHHHPYPINAITRRPYSGINLPLLW
CCHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEE
AEARLQGFTQDRWLTFNQAKKAGGHIRKGEHSTLAVLYKPMEREEQTESGQPVLDENGQP
HHHHHCCCCCCCEEEHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHCCCCCCCCCCCCC
KVAHFGILKTHFLFNIEQTEGLETLNETVLETEPSDPFLPNGAAENLLLRSGANIIHRFA
CHHHHHHHHHHHEEECHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHH
DEAFYHPIRDLIQLPTKAQFHDQGGYYATALHELTHWTGHHTRLQREGVTSSCPFGSPGY
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCH
AFEELIAEMGAAFLCAYAGIQGELRHGGYIDSWLGLLKADKRAIFRASGQARSASEYVLN
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LEQQLRQDVLDDSLCMNDGA
HHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1818755 [H]