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The map label for this gene is ydeM [C]

Identifier: 77459199

GI number: 77459199

Start: 3411859

End: 3413292

Strand: Reverse

Name: ydeM [C]

Synonym: Pfl01_2974

Alternate gene names: 77459199

Gene position: 3413292-3411859 (Counterclockwise)

Preceding gene: 77459200

Following gene: 77459198

Centisome position: 53.01

GC content: 52.44

Gene sequence:

>1434_bases
ATGACAGCGCTTACCCTTATTCGGCATAACGCGCATCAAATAGATGTAGATGGTCATGCCATGATGATGCACGTCCCCAG
CAGCAGTCTTTTTGAGCTTGATGGGGTTGGTCGAGATGTATACGACCTCTTTAGTCGCTTCGATAGCGTCGACAGCGAGG
TCATGCGCACCGAGCTTGGCGCCCGGCATTCTCCGGACAGTCTGGCAGAGTGCATCAGTAGTTTCATCGATCTGGATATC
CTGCGCGAGCCAGGTATTGTCGAAGCGCCTCGGGTGATCGCCAAGGTTGATGAGATACCGCTTTCGACCATTGTTCTTAA
CGTGAATACAGGCTGCAATCTTTCCTGTACTTACTGCTACAAGGAAGACCTCACGACCCCCTCGAAGGGCGTCAAGATGA
GCTTTGAAACCGCCAAGGCCTCGTTTGAGATGTTGCTCAAACAGGCTAAGAATCGGGACCAAGTCAATCTTGTGTTTTTT
GGTGGTGAGCCGCTTTCCAACTATCCACTCATTCGCGCGATGGTGCCTTACGCCGAAGCCCGAGCGGCGCAAGAGGGCAA
GAAGATCGATTTTTCGTTGACCACCAACGGCACGCTGTTAACAGAGCCCATGGTGGATTGGCTCAACGAGCATCGCTTTG
GCTTGACGGTATCGATGGACGGTCCGAAAGCCATGCATGATGCCAACCGCATTACGGTAGGTGGCAACGGAACCTATGAC
GTGGTGGCGCGTAAGGTGCGGATGCTGCTGTCGCGCTATACCGCGAGGGCGGTCGGCGTACGTGTGACACTGACCCGCGG
CGTTACCGATGTGTTGGGTATCCATCATCATCTGAAGAACGAACTGGGATTTGCTGAAGTTGGGTTCGGCCCAGCGACTT
CGGGTCCGATTGCATCGTTCAATCTGGATAACCATGCGCTGAAGCAAGTGTTCAACGATATGAAGACCCTGGGCCAACGG
TATGTCGAGGCGGCTTGCCGTGGCGAAAACATCGGCTTTTCCAACATGCACCAATTGCTGACAGATATTGCACAGGGCAC
TAAAAAAGTCGTGCCCTGTGGTGCCGGTCTGGGATTGCTGGCCATTGATAAGGATGGCGATCTGCATCTGTGTCATCGTT
TCGTTGGGTCTGATCAACCGACCTATGGCAACGTTGATCGCGGTATCGATGTGCCTAAGTTGGCAGCGTTCATTGAAGGC
GCCCAAGACCGCAGTAGTTTCGGGTGTAAAACCTGCCGTATTCGTTCTATTTGTGCGGGCGGGTGTTATCACGAGTCTTA
TGCGAAACAAGGTGATCCGTTTGCTCCTGTTTATCATTACTGTGACTTGTTGCGCGACTGGGTAGATTTCGGAATCGAAT
CTTACGTGCGGATCATGAAAAGTAATCCTAGCTTCTTCCGTAATCATATCGAACCACGGAGCGCCAAAGTATGA

Upstream 100 bases:

>100_bases
ATCGCCACCGTTGATGACAACGGCAAATCTCTCCCGGGCGAGGCGCATCTCTATGTCACCGTCCAGCGTTTCGTCGATAC
TCCTATTCGTTGAGGCTCCC

Downstream 100 bases:

>100_bases
AACATTTGACTCCCGCCAATGCCAAGGCCAAGGCCTTTGTTGAAGCTCACGCCGAAGGTCGTGAAGAAGAAGTTGTAGCG
ATGAACGCCTTGGTAGGCTG

Product: radical SAM family protein

Products: NA

Alternate protein names: AnSME; Cys-type sulfatase-activating enzyme [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MTALTLIRHNAHQIDVDGHAMMMHVPSSSLFELDGVGRDVYDLFSRFDSVDSEVMRTELGARHSPDSLAECISSFIDLDI
LREPGIVEAPRVIAKVDEIPLSTIVLNVNTGCNLSCTYCYKEDLTTPSKGVKMSFETAKASFEMLLKQAKNRDQVNLVFF
GGEPLSNYPLIRAMVPYAEARAAQEGKKIDFSLTTNGTLLTEPMVDWLNEHRFGLTVSMDGPKAMHDANRITVGGNGTYD
VVARKVRMLLSRYTARAVGVRVTLTRGVTDVLGIHHHLKNELGFAEVGFGPATSGPIASFNLDNHALKQVFNDMKTLGQR
YVEAACRGENIGFSNMHQLLTDIAQGTKKVVPCGAGLGLLAIDKDGDLHLCHRFVGSDQPTYGNVDRGIDVPKLAAFIEG
AQDRSSFGCKTCRIRSICAGGCYHESYAKQGDPFAPVYHYCDLLRDWVDFGIESYVRIMKSNPSFFRNHIEPRSAKV

Sequences:

>Translated_477_residues
MTALTLIRHNAHQIDVDGHAMMMHVPSSSLFELDGVGRDVYDLFSRFDSVDSEVMRTELGARHSPDSLAECISSFIDLDI
LREPGIVEAPRVIAKVDEIPLSTIVLNVNTGCNLSCTYCYKEDLTTPSKGVKMSFETAKASFEMLLKQAKNRDQVNLVFF
GGEPLSNYPLIRAMVPYAEARAAQEGKKIDFSLTTNGTLLTEPMVDWLNEHRFGLTVSMDGPKAMHDANRITVGGNGTYD
VVARKVRMLLSRYTARAVGVRVTLTRGVTDVLGIHHHLKNELGFAEVGFGPATSGPIASFNLDNHALKQVFNDMKTLGQR
YVEAACRGENIGFSNMHQLLTDIAQGTKKVVPCGAGLGLLAIDKDGDLHLCHRFVGSDQPTYGNVDRGIDVPKLAAFIEG
AQDRSSFGCKTCRIRSICAGGCYHESYAKQGDPFAPVYHYCDLLRDWVDFGIESYVRIMKSNPSFFRNHIEPRSAKV
>Mature_476_residues
TALTLIRHNAHQIDVDGHAMMMHVPSSSLFELDGVGRDVYDLFSRFDSVDSEVMRTELGARHSPDSLAECISSFIDLDIL
REPGIVEAPRVIAKVDEIPLSTIVLNVNTGCNLSCTYCYKEDLTTPSKGVKMSFETAKASFEMLLKQAKNRDQVNLVFFG
GEPLSNYPLIRAMVPYAEARAAQEGKKIDFSLTTNGTLLTEPMVDWLNEHRFGLTVSMDGPKAMHDANRITVGGNGTYDV
VARKVRMLLSRYTARAVGVRVTLTRGVTDVLGIHHHLKNELGFAEVGFGPATSGPIASFNLDNHALKQVFNDMKTLGQRY
VEAACRGENIGFSNMHQLLTDIAQGTKKVVPCGAGLGLLAIDKDGDLHLCHRFVGSDQPTYGNVDRGIDVPKLAAFIEGA
QDRSSFGCKTCRIRSICAGGCYHESYAKQGDPFAPVYHYCDLLRDWVDFGIESYVRIMKSNPSFFRNHIEPRSAKV

Specific function: Involved in 'Cys-type' sulfatase maturation under anaerobic conditions. Catalyzes the post-translational modification of cysteine ('Cys-51' in the arylsulfatase CPF_0221) into 3-oxoalanine (also known as C(alpha)-formylglycine (FGly)), by a free radical c

COG id: COG0641

COG function: function code R; Arylsulfatase regulator (Fe-S oxidoreductase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Anaerobic sulfatase-maturating enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081923, Length=337, Percent_Identity=26.1127596439169, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI48994973, Length=350, Percent_Identity=25.4285714285714, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 52606; Mature: 52474

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALTLIRHNAHQIDVDGHAMMMHVPSSSLFELDGVGRDVYDLFSRFDSVDSEVMRTELG
CCEEEEEECCCEEEECCCCEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHC
ARHSPDSLAECISSFIDLDILREPGIVEAPRVIAKVDEIPLSTIVLNVNTGCNLSCTYCY
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEEEEEECCCCCEEEEEEE
KEDLTTPSKGVKMSFETAKASFEMLLKQAKNRDQVNLVFFGGEPLSNYPLIRAMVPYAEA
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHCCHHHH
RAAQEGKKIDFSLTTNGTLLTEPMVDWLNEHRFGLTVSMDGPKAMHDANRITVGGNGTYD
HHHHCCCEEEEEEECCCEEECHHHHHHHHCCCCEEEEECCCCHHHCCCCEEEECCCCCHH
VVARKVRMLLSRYTARAVGVRVTLTRGVTDVLGIHHHLKNELGFAEVGFGPATSGPIASF
HHHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHCCEEECCCCCCCCCCEEEE
NLDNHALKQVFNDMKTLGQRYVEAACRGENIGFSNMHQLLTDIAQGTKKVVPCGAGLGLL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEE
AIDKDGDLHLCHRFVGSDQPTYGNVDRGIDVPKLAAFIEGAQDRSSFGCKTCRIRSICAG
EECCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHCC
GCYHESYAKQGDPFAPVYHYCDLLRDWVDFGIESYVRIMKSNPSFFRNHIEPRSAKV
CCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TALTLIRHNAHQIDVDGHAMMMHVPSSSLFELDGVGRDVYDLFSRFDSVDSEVMRTELG
CEEEEEECCCEEEECCCCEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHC
ARHSPDSLAECISSFIDLDILREPGIVEAPRVIAKVDEIPLSTIVLNVNTGCNLSCTYCY
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEEEEEECCCCCEEEEEEE
KEDLTTPSKGVKMSFETAKASFEMLLKQAKNRDQVNLVFFGGEPLSNYPLIRAMVPYAEA
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHCCHHHH
RAAQEGKKIDFSLTTNGTLLTEPMVDWLNEHRFGLTVSMDGPKAMHDANRITVGGNGTYD
HHHHCCCEEEEEEECCCEEECHHHHHHHHCCCCEEEEECCCCHHHCCCCEEEECCCCCHH
VVARKVRMLLSRYTARAVGVRVTLTRGVTDVLGIHHHLKNELGFAEVGFGPATSGPIASF
HHHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHCCEEECCCCCCCCCCEEEE
NLDNHALKQVFNDMKTLGQRYVEAACRGENIGFSNMHQLLTDIAQGTKKVVPCGAGLGLL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEE
AIDKDGDLHLCHRFVGSDQPTYGNVDRGIDVPKLAAFIEGAQDRSSFGCKTCRIRSICAG
EECCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHCC
GCYHESYAKQGDPFAPVYHYCDLLRDWVDFGIESYVRIMKSNPSFFRNHIEPRSAKV
CCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA