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The map label for this gene is pncA [H]

Identifier: 77459112

GI number: 77459112

Start: 3327072

End: 3327698

Strand: Direct

Name: pncA [H]

Synonym: Pfl01_2887

Alternate gene names: 77459112

Gene position: 3327072-3327698 (Clockwise)

Preceding gene: 77459111

Following gene: 77459113

Centisome position: 51.68

GC content: 62.52

Gene sequence:

>627_bases
ATGCCGATTTCGCCACGCACCGCCCTGCTGGTCATCGACGTGCAGAACGACTTCATTCCCGGCGGCCAGTTGGCCGTGCC
CGAAGGTGACCTGATCGTCCCGCTGATCAACCGGCTTGCCGGGCAGTTCAAGCAAGTGGTGATCGCCCAGGACTGGCACC
CGGCCGGGCATGCTTCGTTTGCCTCCAGCCACCCCGGCCGCCAGCCTTACGACGTGATTCAACTGCCGTATGGCGAGCAG
ACACTGTGGCCGGATCACTGCATTCAGGGCTCACGCGGTGCCGAGTTTCACTCGGGACTCGATCTGCCCCATGCGCAATT
GATCATCCGCAAGGGCTGCAACCCGGACATCGACAGCTATTCGGCGTTTCTGGAAGCGGATCGCGTCACGACCACGGGTC
TGGCGGGTTATCTGAAGGAACGCGGCATCGACACGGTGTACATGGTCGGCCTGGCGCTGGATTTTTGCGTGATGTTCTCC
GCCTTGGACGCGCGAGCGTCGGGGTTCAATGCGTTTGTGGTGCTGGATGCTTGCCGGGCGATTGATTTGAACGGCTCGCT
GGCGGCAGCGATCGAGCGGATGCAGGTGGCCGGGGTCGGGCTGATCCAGTCGACTGAACTGCTCTGA

Upstream 100 bases:

>100_bases
CCGAGCTGGAAGACATGGCGAAAATGTTTAAGACCCGGCTGAACGTTTAAAGAATTTTGTCACTATGCTGCTCGGGTGAT
TTTCCCAGAAGGATGGATTT

Downstream 100 bases:

>100_bases
TGATGCCCGTTCCCGCCTAGCGCACTGATCGTTCCCACACTCTGGACTGACCGCGGTCGCGGGGAATTAGGCTCTGTAAC
TGTTGTGCGAGGCAATTCGG

Product: nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MPISPRTALLVIDVQNDFIPGGQLAVPEGDLIVPLINRLAGQFKQVVIAQDWHPAGHASFASSHPGRQPYDVIQLPYGEQ
TLWPDHCIQGSRGAEFHSGLDLPHAQLIIRKGCNPDIDSYSAFLEADRVTTTGLAGYLKERGIDTVYMVGLALDFCVMFS
ALDARASGFNAFVVLDACRAIDLNGSLAAAIERMQVAGVGLIQSTELL

Sequences:

>Translated_208_residues
MPISPRTALLVIDVQNDFIPGGQLAVPEGDLIVPLINRLAGQFKQVVIAQDWHPAGHASFASSHPGRQPYDVIQLPYGEQ
TLWPDHCIQGSRGAEFHSGLDLPHAQLIIRKGCNPDIDSYSAFLEADRVTTTGLAGYLKERGIDTVYMVGLALDFCVMFS
ALDARASGFNAFVVLDACRAIDLNGSLAAAIERMQVAGVGLIQSTELL
>Mature_207_residues
PISPRTALLVIDVQNDFIPGGQLAVPEGDLIVPLINRLAGQFKQVVIAQDWHPAGHASFASSHPGRQPYDVIQLPYGEQT
LWPDHCIQGSRGAEFHSGLDLPHAQLIIRKGCNPDIDSYSAFLEADRVTTTGLAGYLKERGIDTVYMVGLALDFCVMFSA
LDARASGFNAFVVLDACRAIDLNGSLAAAIERMQVAGVGLIQSTELL

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=202, Percent_Identity=44.5544554455446, Blast_Score=166, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI193206735, Length=231, Percent_Identity=35.9307359307359, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17543130, Length=231, Percent_Identity=35.9307359307359, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71999339, Length=221, Percent_Identity=32.1266968325792, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321401, Length=215, Percent_Identity=31.1627906976744, Blast_Score=113, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648247, Length=228, Percent_Identity=31.5789473684211, Blast_Score=125, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 22400; Mature: 22269

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPISPRTALLVIDVQNDFIPGGQLAVPEGDLIVPLINRLAGQFKQVVIAQDWHPAGHASF
CCCCCCEEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHEEEECCCCCCCCCCC
ASSHPGRQPYDVIQLPYGEQTLWPDHCIQGSRGAEFHSGLDLPHAQLIIRKGCNPDIDSY
CCCCCCCCCCEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHH
SAFLEADRVTTTGLAGYLKERGIDTVYMVGLALDFCVMFSALDARASGFNAFVVLDACRA
HHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHE
IDLNGSLAAAIERMQVAGVGLIQSTELL
ECCCCHHHHHHHHHHHHCCCEECCCCCC
>Mature Secondary Structure 
PISPRTALLVIDVQNDFIPGGQLAVPEGDLIVPLINRLAGQFKQVVIAQDWHPAGHASF
CCCCCEEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHEEEECCCCCCCCCCC
ASSHPGRQPYDVIQLPYGEQTLWPDHCIQGSRGAEFHSGLDLPHAQLIIRKGCNPDIDSY
CCCCCCCCCCEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHH
SAFLEADRVTTTGLAGYLKERGIDTVYMVGLALDFCVMFSALDARASGFNAFVVLDACRA
HHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHE
IDLNGSLAAAIERMQVAGVGLIQSTELL
ECCCCHHHHHHHHHHHHCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]