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The map label for this gene is puuB [H]

Identifier: 77459055

GI number: 77459055

Start: 3263287

End: 3264588

Strand: Direct

Name: puuB [H]

Synonym: Pfl01_2830

Alternate gene names: 77459055

Gene position: 3263287-3264588 (Clockwise)

Preceding gene: 77459054

Following gene: 77459056

Centisome position: 50.68

GC content: 65.13

Gene sequence:

>1302_bases
ATGAATCAGTACACCCAGGAACACGCCCAGTCCTATTACGCCGCGTCGGCCCGGGCCAGCACACCCTATCCGGAACTGGC
GGGCGACCTGATCGCCGATGTCTGCGTGATCGGCGGCGGGTTCACCGGCGTCAACACGGCCATTGAACTGGCGCAGCGCG
GGCTTTCGGTGATTCTGCTCGAAGCCCGGCGCATCGGCTGGGGCGCCAGCGGGCGCAATGGCGGGCAGCTGATTCGCGGG
ATCGGCCATGACGTCGAAGGTTTCTCACGGCATGTCGGCAGCGAAGGCGTGCGCTATCTGCACCGGGCCGGGATCGATTC
GGTGGAGCTGGTGCGCCAGCGCATCACCGACAACGCCATCGAGTGCGACCTGCGCTGGGGCTTCTGCGAGCTAGCCAACA
CCCCGGCGCAGTTCGACGCCTTCAAGGCCGAGCAGGACAGCCTGGCGCAGCTGGGTTACCGCCATGAAACGCGGCTGGTC
GCCCCTGAGCTGATTCGTCAGCAAGTTGTGAACGCGGGCGTCTACAAGGGCGGCCTGATCGACAAGGGCTCAGGGCATCT
GCACCCGCTCGACCTGGTCCAGGGCGAAGCCCGACTGGCAGCCTCCCTCGGGGTGCGGATTTTCGAGCAGAGCCCGGTGC
TGGAAATTCTCCACGGTTCGACCGTTCAAGTCCGCACCCAGAGCGGCACGGTGCGCGCCGGCAGTCTTGTGCTCGGCTGC
AACGCGCATCTGGATGAACTTGAACAGCAACTCAGCGGCAAAGTGCTGCCCGCTGGCAGTTACATCATCGCCACTGAACC
GCTGTCGGCGGAACGCGCCGCCGAGCTGATTCCGCAGAACCTGGCGCTGTGCGATCAGAAAGTCGGCCTCGACTACTACC
GGCTCTCGGCGGACCGGCGCTTGCTGTTCGGCGGCGCCTGCCATTATTCGGGACGGGACCCGGCGGACATCGCCGCGTAC
ATGCGACCAAAGATGCTCAAGGTCTTCCCGCAACTGGCGGATGTGCGCATCGACTATCAGTGGGGCGGCAAGATCGGCAT
CACCGCCAACCGCTTCCCACAGGTCGGCCGTTTGAAGCAGCACCCGAACGTGTTCTACGCCCAGGGCTATTCCGGTCACG
GTCTGAACGTCACCCACTGGTGCGCCAGGCTGCTGGGCGAAGCGATTCATGCCGGCCACAGTCAGGGCATGGACGTGTTC
AGCAATGTGCCGCACATGACCTTCCCCGGCGGCCCGGCGCTGCGCTCGCCACTGCTGGCACTGGGTATGTTCTGGTATCG
CCTGCGGGAGCTGCTGGGATGA

Upstream 100 bases:

>100_bases
CGTCTCAGGAAGTCCTCGACAAGCTCTACATCTCCTCTACCCCGCCCCAGTCGATCATGCGTCTGATGACCCGGTCCTGG
AGCAAAGTGAAGTCGAACAA

Downstream 100 bases:

>100_bases
CGCTTCGCGGTTAAAGCATCCTGCACATTTGCTCTATCGCGAAGCACTTCTATATTGATGAAGTGCTTTTGCGTTCCTGA
CGCTCAGTGCCCACACACCC

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MNQYTQEHAQSYYAASARASTPYPELAGDLIADVCVIGGGFTGVNTAIELAQRGLSVILLEARRIGWGASGRNGGQLIRG
IGHDVEGFSRHVGSEGVRYLHRAGIDSVELVRQRITDNAIECDLRWGFCELANTPAQFDAFKAEQDSLAQLGYRHETRLV
APELIRQQVVNAGVYKGGLIDKGSGHLHPLDLVQGEARLAASLGVRIFEQSPVLEILHGSTVQVRTQSGTVRAGSLVLGC
NAHLDELEQQLSGKVLPAGSYIIATEPLSAERAAELIPQNLALCDQKVGLDYYRLSADRRLLFGGACHYSGRDPADIAAY
MRPKMLKVFPQLADVRIDYQWGGKIGITANRFPQVGRLKQHPNVFYAQGYSGHGLNVTHWCARLLGEAIHAGHSQGMDVF
SNVPHMTFPGGPALRSPLLALGMFWYRLRELLG

Sequences:

>Translated_433_residues
MNQYTQEHAQSYYAASARASTPYPELAGDLIADVCVIGGGFTGVNTAIELAQRGLSVILLEARRIGWGASGRNGGQLIRG
IGHDVEGFSRHVGSEGVRYLHRAGIDSVELVRQRITDNAIECDLRWGFCELANTPAQFDAFKAEQDSLAQLGYRHETRLV
APELIRQQVVNAGVYKGGLIDKGSGHLHPLDLVQGEARLAASLGVRIFEQSPVLEILHGSTVQVRTQSGTVRAGSLVLGC
NAHLDELEQQLSGKVLPAGSYIIATEPLSAERAAELIPQNLALCDQKVGLDYYRLSADRRLLFGGACHYSGRDPADIAAY
MRPKMLKVFPQLADVRIDYQWGGKIGITANRFPQVGRLKQHPNVFYAQGYSGHGLNVTHWCARLLGEAIHAGHSQGMDVF
SNVPHMTFPGGPALRSPLLALGMFWYRLRELLG
>Mature_433_residues
MNQYTQEHAQSYYAASARASTPYPELAGDLIADVCVIGGGFTGVNTAIELAQRGLSVILLEARRIGWGASGRNGGQLIRG
IGHDVEGFSRHVGSEGVRYLHRAGIDSVELVRQRITDNAIECDLRWGFCELANTPAQFDAFKAEQDSLAQLGYRHETRLV
APELIRQQVVNAGVYKGGLIDKGSGHLHPLDLVQGEARLAASLGVRIFEQSPVLEILHGSTVQVRTQSGTVRAGSLVLGC
NAHLDELEQQLSGKVLPAGSYIIATEPLSAERAAELIPQNLALCDQKVGLDYYRLSADRRLLFGGACHYSGRDPADIAAY
MRPKMLKVFPQLADVRIDYQWGGKIGITANRFPQVGRLKQHPNVFYAQGYSGHGLNVTHWCARLLGEAIHAGHSQGMDVF
SNVPHMTFPGGPALRSPLLALGMFWYRLRELLG

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=428, Percent_Identity=41.8224299065421, Blast_Score=341, Evalue=4e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47184; Mature: 47184

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQYTQEHAQSYYAASARASTPYPELAGDLIADVCVIGGGFTGVNTAIELAQRGLSVILL
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEE
EARRIGWGASGRNGGQLIRGIGHDVEGFSRHVGSEGVRYLHRAGIDSVELVRQRITDNAI
EHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHCCCCE
ECDLRWGFCELANTPAQFDAFKAEQDSLAQLGYRHETRLVAPELIRQQVVNAGVYKGGLI
EEEEECCHHHHCCCCHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCE
DKGSGHLHPLDLVQGEARLAASLGVRIFEQSPVLEILHGSTVQVRTQSGTVRAGSLVLGC
ECCCCCCCHHHHHCCHHHHHHHHCEEEECCCCHHHHHCCCEEEEEECCCCEEECCEEEEC
NAHLDELEQQLSGKVLPAGSYIIATEPLSAERAAELIPQNLALCDQKVGLDYYRLSADRR
CCCHHHHHHHHCCCEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCE
LLFGGACHYSGRDPADIAAYMRPKMLKVFPQLADVRIDYQWGGKIGITANRFPQVGRLKQ
EEEECEECCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEEECCEEECCCCCCCCCCCHHC
HPNVFYAQGYSGHGLNVTHWCARLLGEAIHAGHSQGMDVFSNVPHMTFPGGPALRSPLLA
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCHHHHHHHHH
LGMFWYRLRELLG
HHHHHHHHHHHHC
>Mature Secondary Structure
MNQYTQEHAQSYYAASARASTPYPELAGDLIADVCVIGGGFTGVNTAIELAQRGLSVILL
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEE
EARRIGWGASGRNGGQLIRGIGHDVEGFSRHVGSEGVRYLHRAGIDSVELVRQRITDNAI
EHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHCCCCE
ECDLRWGFCELANTPAQFDAFKAEQDSLAQLGYRHETRLVAPELIRQQVVNAGVYKGGLI
EEEEECCHHHHCCCCHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCE
DKGSGHLHPLDLVQGEARLAASLGVRIFEQSPVLEILHGSTVQVRTQSGTVRAGSLVLGC
ECCCCCCCHHHHHCCHHHHHHHHCEEEECCCCHHHHHCCCEEEEEECCCCEEECCEEEEC
NAHLDELEQQLSGKVLPAGSYIIATEPLSAERAAELIPQNLALCDQKVGLDYYRLSADRR
CCCHHHHHHHHCCCEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCE
LLFGGACHYSGRDPADIAAYMRPKMLKVFPQLADVRIDYQWGGKIGITANRFPQVGRLKQ
EEEECEECCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEEECCEEECCCCCCCCCCCHHC
HPNVFYAQGYSGHGLNVTHWCARLLGEAIHAGHSQGMDVFSNVPHMTFPGGPALRSPLLA
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCHHHHHHHHH
LGMFWYRLRELLG
HHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]