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The map label for this gene is wcaJ [H]

Identifier: 77459041

GI number: 77459041

Start: 3246446

End: 3247837

Strand: Direct

Name: wcaJ [H]

Synonym: Pfl01_2816

Alternate gene names: 77459041

Gene position: 3246446-3247837 (Clockwise)

Preceding gene: 77459040

Following gene: 77459042

Centisome position: 50.42

GC content: 60.34

Gene sequence:

>1392_bases
ATGACTTCGCTCTATACCGCTCACATGACACACCGCCGAGGCCTGACATTCTGGGGCCAGTGGTTGTGCGCGCTGTCGCT
GGTCACCGCGCTGTTGATATTGCTCGTGCACTGGCGCGTCGGCAGTCTGACCAGCGAATACCGCATCCTGATCATTCTGA
CGGTGCTCGGCTCGATACCGATCTACAGCGTGATGCAGGTCTACCACAAACGCCATGGCTTGCTGGTCGGGCTTGGCAGG
TTGCTGGCCGGGTGGTTGACCCTGCTGGCCGTACTGGCGGCGATTGCGTTCTTCACCCAGACCAGCGCCATTTATTCACG
CCAGGTGATCATGGTCTGGGCGGTGCTGGGTTTCGTGGTGCAGGCCTTGAGCTTTCTGCCCCTGCATTACCTGGCCCGCC
TGTACTCGCGCAAGGTCTGCAACGGACGTCGTTCGGTGATCATCGGTACCTGCCCGACGGCCCATGAACTGGCGAAAAAA
CTGTCCCGACCGAACCGCGCGCTGGTGCTGGGTTTCATTGCCGCCGAGGACAGCTGCGGCCCTGCCCCCAGCATTCTGCC
GTTGCTCGGCCACGTTTCCGCTATCCGCGAGATCATCACGGGCCTGGAAGTGCGTCGCGTCTATATCGTGCTGCCGATGG
CCCATGCAGCAACCATCGAAGCGCTTTATATCGACCTGCTGGACATGAATGTCGACGTGGTGTGGATCCCGGATTTTGGC
AGCATGGTGTTGCTCAACCATTCGATCTCGGAAATCGAGCGAATGCCGGCCATCTACCTCAACGAGAGCCTGATCAGTTC
GCACCCCGCCAGCCTGTTTTGCAAGGATCTGTTTGAGCGAAGCCTCGCCGCCCTGGCCATTTTTCTGCTCAGCCCCGTGC
TGCTGGTGGTCGCCGTTGCCGTCAAGCTGACCTCTGCAGGGCCAGTGCTGTTCAAGCAGAACCGTCACGGTTGCAACGGC
GAAGTGATCAAGGTCTGGAAGTTTCGCTCGATGCACGTCCACGACGACCAGGAGGTACGCCAGGCCACCCGCAACGATGA
CCGCCTCACGCCGATCGGCGGTTTCCTGCGCCGCAGTTCGATCGACGAATTGCCACAGCTGTTCAATGTCCTGTCCGGCC
GGATGGCCCTGGTCGGCCCGCGTCCGCATGCCGTCACCCACAACCTTTATTACACCGGCAAGGTGCGCGCCTACATGGCC
CGGCATCGCCTCAAGCCGGGTATCACCGGGCTGGCCCAGATCACTGGCCATCGTGGCGAAACCGAGACCGTGGAAAAGAT
GCAGCTGCGCGTCGCCCAGGACCTCAACTACATCAACCAGTGGTCGCTGTGGCTCGACATCAAAATCCTCATCAAGACGC
CCTTCACCCTGTTCTCGAAAAACATCTACTGA

Upstream 100 bases:

>100_bases
ACTCGCTTCACCTCCGCCGAGCTACGGAAAAGCCATCGGGAGGCACGCCGTGCCCGCTCGATTCGCGCTGATGCATGAAT
GCTGTCTTGAGGTTAAGCCT

Downstream 100 bases:

>100_bases
GCCCCTTTCCCGCGTCTGCCGCTCTCCTGCCCGACCGTTGATAACCGTCACGACGACCTGAGGTTCGACCATGAATGTGA
GCGTATTCGGTATTGGTTAC

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MTSLYTAHMTHRRGLTFWGQWLCALSLVTALLILLVHWRVGSLTSEYRILIILTVLGSIPIYSVMQVYHKRHGLLVGLGR
LLAGWLTLLAVLAAIAFFTQTSAIYSRQVIMVWAVLGFVVQALSFLPLHYLARLYSRKVCNGRRSVIIGTCPTAHELAKK
LSRPNRALVLGFIAAEDSCGPAPSILPLLGHVSAIREIITGLEVRRVYIVLPMAHAATIEALYIDLLDMNVDVVWIPDFG
SMVLLNHSISEIERMPAIYLNESLISSHPASLFCKDLFERSLAALAIFLLSPVLLVVAVAVKLTSAGPVLFKQNRHGCNG
EVIKVWKFRSMHVHDDQEVRQATRNDDRLTPIGGFLRRSSIDELPQLFNVLSGRMALVGPRPHAVTHNLYYTGKVRAYMA
RHRLKPGITGLAQITGHRGETETVEKMQLRVAQDLNYINQWSLWLDIKILIKTPFTLFSKNIY

Sequences:

>Translated_463_residues
MTSLYTAHMTHRRGLTFWGQWLCALSLVTALLILLVHWRVGSLTSEYRILIILTVLGSIPIYSVMQVYHKRHGLLVGLGR
LLAGWLTLLAVLAAIAFFTQTSAIYSRQVIMVWAVLGFVVQALSFLPLHYLARLYSRKVCNGRRSVIIGTCPTAHELAKK
LSRPNRALVLGFIAAEDSCGPAPSILPLLGHVSAIREIITGLEVRRVYIVLPMAHAATIEALYIDLLDMNVDVVWIPDFG
SMVLLNHSISEIERMPAIYLNESLISSHPASLFCKDLFERSLAALAIFLLSPVLLVVAVAVKLTSAGPVLFKQNRHGCNG
EVIKVWKFRSMHVHDDQEVRQATRNDDRLTPIGGFLRRSSIDELPQLFNVLSGRMALVGPRPHAVTHNLYYTGKVRAYMA
RHRLKPGITGLAQITGHRGETETVEKMQLRVAQDLNYINQWSLWLDIKILIKTPFTLFSKNIY
>Mature_462_residues
TSLYTAHMTHRRGLTFWGQWLCALSLVTALLILLVHWRVGSLTSEYRILIILTVLGSIPIYSVMQVYHKRHGLLVGLGRL
LAGWLTLLAVLAAIAFFTQTSAIYSRQVIMVWAVLGFVVQALSFLPLHYLARLYSRKVCNGRRSVIIGTCPTAHELAKKL
SRPNRALVLGFIAAEDSCGPAPSILPLLGHVSAIREIITGLEVRRVYIVLPMAHAATIEALYIDLLDMNVDVVWIPDFGS
MVLLNHSISEIERMPAIYLNESLISSHPASLFCKDLFERSLAALAIFLLSPVLLVVAVAVKLTSAGPVLFKQNRHGCNGE
VIKVWKFRSMHVHDDQEVRQATRNDDRLTPIGGFLRRSSIDELPQLFNVLSGRMALVGPRPHAVTHNLYYTGKVRAYMAR
HRLKPGITGLAQITGHRGETETVEKMQLRVAQDLNYINQWSLWLDIKILIKTPFTLFSKNIY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=270, Percent_Identity=46.6666666666667, Blast_Score=247, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 51895; Mature: 51764

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLYTAHMTHRRGLTFWGQWLCALSLVTALLILLVHWRVGSLTSEYRILIILTVLGSIP
CCCEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCH
IYSVMQVYHKRHGLLVGLGRLLAGWLTLLAVLAAIAFFTQTSAIYSRQVIMVWAVLGFVV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QALSFLPLHYLARLYSRKVCNGRRSVIIGTCPTAHELAKKLSRPNRALVLGFIAAEDSCG
HHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCCEEEEEEEEECCCCC
PAPSILPLLGHVSAIREIITGLEVRRVYIVLPMAHAATIEALYIDLLDMNVDVVWIPDFG
CCCHHHHHHHHHHHHHHHHCCHHEEEEEEEEECHHHHHHHHHHHHHEECCEEEEEECCCC
SMVLLNHSISEIERMPAIYLNESLISSHPASLFCKDLFERSLAALAIFLLSPVLLVVAVA
CEEEECCCHHHHHHCCHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKLTSAGPVLFKQNRHGCNGEVIKVWKFRSMHVHDDQEVRQATRNDDRLTPIGGFLRRSS
HHHCCCCCEEEECCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCC
IDELPQLFNVLSGRMALVGPRPHAVTHNLYYTGKVRAYMARHRLKPGITGLAQITGHRGE
HHHHHHHHHHHCCCEEEECCCCCEEEECEEEECHHHHHHHHHCCCCCCCHHHHHCCCCCC
TETVEKMQLRVAQDLNYINQWSLWLDIKILIKTPFTLFSKNIY
HHHHHHHHHHHHHHCCHHHCEEEEEEEEEEEECCHHHHCCCCC
>Mature Secondary Structure 
TSLYTAHMTHRRGLTFWGQWLCALSLVTALLILLVHWRVGSLTSEYRILIILTVLGSIP
CCEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCH
IYSVMQVYHKRHGLLVGLGRLLAGWLTLLAVLAAIAFFTQTSAIYSRQVIMVWAVLGFVV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QALSFLPLHYLARLYSRKVCNGRRSVIIGTCPTAHELAKKLSRPNRALVLGFIAAEDSCG
HHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCCEEEEEEEEECCCCC
PAPSILPLLGHVSAIREIITGLEVRRVYIVLPMAHAATIEALYIDLLDMNVDVVWIPDFG
CCCHHHHHHHHHHHHHHHHCCHHEEEEEEEEECHHHHHHHHHHHHHEECCEEEEEECCCC
SMVLLNHSISEIERMPAIYLNESLISSHPASLFCKDLFERSLAALAIFLLSPVLLVVAVA
CEEEECCCHHHHHHCCHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKLTSAGPVLFKQNRHGCNGEVIKVWKFRSMHVHDDQEVRQATRNDDRLTPIGGFLRRSS
HHHCCCCCEEEECCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCC
IDELPQLFNVLSGRMALVGPRPHAVTHNLYYTGKVRAYMARHRLKPGITGLAQITGHRGE
HHHHHHHHHHHCCCEEEECCCCCEEEECEEEECHHHHHHHHHCCCCCCCHHHHHCCCCCC
TETVEKMQLRVAQDLNYINQWSLWLDIKILIKTPFTLFSKNIY
HHHHHHHHHHHHHHCCHHHCEEEEEEEEEEEECCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]