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The map label for this gene is actII-2 [H]

Identifier: 77458951

GI number: 77458951

Start: 3142212

End: 3143666

Strand: Reverse

Name: actII-2 [H]

Synonym: Pfl01_2726

Alternate gene names: 77458951

Gene position: 3143666-3142212 (Counterclockwise)

Preceding gene: 77458953

Following gene: 77458948

Centisome position: 48.83

GC content: 61.31

Gene sequence:

>1455_bases
ATGTCTCGAATCGAAATCGAAGTGCCCGAACCCCGGCGCTGGTTGATGTTTGCCATATTGCTCGTCGGAGCGTTTCTGCC
GCCCCTGGATTTTTTTATCGTCAACGTCGCCCTGCCCTCGATGCAGGAGGATCTGGGGACAGGCTCCTCTGCCGAGCAGT
TGGTGATTTCTTCCTATGCAACGCTCTATGCCGTGACGCTGATTACCGGCGGACGACTCGGCGATCTGTACGGCCGCGGG
CGGATGTTTTTCCTTGGCCTGCTGGGTTTCGCCGCCGCGTCGCTGATGTGCGGCCTGGCGGTTTCGCCGTGGATGCTCAT
CCTCGGACGCGCCTTGCAGGGTGTGACCGCCGCCGTAATGGCCCCTCAGGCACTTGCATCGGTGCAGGCGATTTTTCCAG
TGTCGGAAAGACCATTGGCATTGAGCATTTACGGGGCCGTATTTGGACTGGCGTCTGTGATCGGTCAGGCACTGGGCGGC
TTTTTGATTGAGATCGACGTGTTGGGCATGGGATGGCGCGCGATCTTCCTGGTCAACCTGCCGATCGCGTTGCTGGTGAT
TGCGTTCGGCATCCGTGTGCTCAAAGAAACCCGTGCGCAGCACGCAAGCAAGCTGGATCTGGTGGGCACTGCGTTGTCGA
TGCTGACCCTGACCACCCTGGTCGTGCCATTGATTGAAGGCCGCGAGGCCGGATGGCCGTGGTGGGCATGGCTGTCGCTG
GCCGCCAGCCCTGCGCTGGCGGTAGCGTTCTGGCGCTACGAGAGCCGCTTGCACCGGGCCGGAGGAACGCCCATCCTCGC
CCCGGCCGTACTGAATGCACCGGGCCTGGGGCGAGCGCTTCTGGTCGCGCTTTGCTTCTATTCAATCGGTGTGTTTTTTC
TGATGTTCTCGATGTACATGCAAAGCGCATTGCATATGAGCGCACTGCACGCCGGCCTGATCTTTCTGCCGTTTGGCGCG
GGATTTCTGCTCGGTCCCCTGTCGGTTCCGCTCCTCAGGCGCATCCTCGGGGGCTACGTGAATCCTGTAGGCATGGGACT
GGAAGTAGTCGGATTACTATGGCTGGGCTGGCTCGTGTCAGTGACACCGACTACCCTCGCCCCGCCCTTCACTTCTCTGA
CGGTCATCCTGTTCCTGATCGGTCTGGGACAGGGGCTTGGCCTGCCGACGTTGATGCGCATGGTCACGGGCCGGGTAGCG
CCGGCGTATGCCGGGATGATCGCAGGCATTACCAGCGCCACGCTGCAGATCAGCACATCGCTGAGCGTGGCACTGATTGG
CGGTGTGTTTTATACCTTGCTGGGAGAACCGGCAACGGCGGTCAGCATCACTCATGCGTTCACGATCGCTGTTCTGTGCA
TCGCCGTCTGCCTGGCCGTAGGGACAGCGATAAGCCTGACGTTAGCGCGCGAGCCAGCGACGGTAGCGCAAGCCTCGGCT
GTACGCCCGACCTGA

Upstream 100 bases:

>100_bases
AGCGATTCCCTAAATTGGGACCCGGCGACCTGACCCGGGCAGGCGCGAAGCCTTGCCTTTTAGTCGTCGTCAAACCTGAG
TTCCCTACAGGATGCCTGTC

Downstream 100 bases:

>100_bases
GGCGCGCTACCCCGTACCGATCATGCTGGAGGCAAGGTCGGCCAGTGCGCGCAGCCGGTCCATCTGGCGCATGTCCTGGT
GATACCCCATCCAGATGTCC

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MSRIEIEVPEPRRWLMFAILLVGAFLPPLDFFIVNVALPSMQEDLGTGSSAEQLVISSYATLYAVTLITGGRLGDLYGRG
RMFFLGLLGFAAASLMCGLAVSPWMLILGRALQGVTAAVMAPQALASVQAIFPVSERPLALSIYGAVFGLASVIGQALGG
FLIEIDVLGMGWRAIFLVNLPIALLVIAFGIRVLKETRAQHASKLDLVGTALSMLTLTTLVVPLIEGREAGWPWWAWLSL
AASPALAVAFWRYESRLHRAGGTPILAPAVLNAPGLGRALLVALCFYSIGVFFLMFSMYMQSALHMSALHAGLIFLPFGA
GFLLGPLSVPLLRRILGGYVNPVGMGLEVVGLLWLGWLVSVTPTTLAPPFTSLTVILFLIGLGQGLGLPTLMRMVTGRVA
PAYAGMIAGITSATLQISTSLSVALIGGVFYTLLGEPATAVSITHAFTIAVLCIAVCLAVGTAISLTLAREPATVAQASA
VRPT

Sequences:

>Translated_484_residues
MSRIEIEVPEPRRWLMFAILLVGAFLPPLDFFIVNVALPSMQEDLGTGSSAEQLVISSYATLYAVTLITGGRLGDLYGRG
RMFFLGLLGFAAASLMCGLAVSPWMLILGRALQGVTAAVMAPQALASVQAIFPVSERPLALSIYGAVFGLASVIGQALGG
FLIEIDVLGMGWRAIFLVNLPIALLVIAFGIRVLKETRAQHASKLDLVGTALSMLTLTTLVVPLIEGREAGWPWWAWLSL
AASPALAVAFWRYESRLHRAGGTPILAPAVLNAPGLGRALLVALCFYSIGVFFLMFSMYMQSALHMSALHAGLIFLPFGA
GFLLGPLSVPLLRRILGGYVNPVGMGLEVVGLLWLGWLVSVTPTTLAPPFTSLTVILFLIGLGQGLGLPTLMRMVTGRVA
PAYAGMIAGITSATLQISTSLSVALIGGVFYTLLGEPATAVSITHAFTIAVLCIAVCLAVGTAISLTLAREPATVAQASA
VRPT
>Mature_483_residues
SRIEIEVPEPRRWLMFAILLVGAFLPPLDFFIVNVALPSMQEDLGTGSSAEQLVISSYATLYAVTLITGGRLGDLYGRGR
MFFLGLLGFAAASLMCGLAVSPWMLILGRALQGVTAAVMAPQALASVQAIFPVSERPLALSIYGAVFGLASVIGQALGGF
LIEIDVLGMGWRAIFLVNLPIALLVIAFGIRVLKETRAQHASKLDLVGTALSMLTLTTLVVPLIEGREAGWPWWAWLSLA
ASPALAVAFWRYESRLHRAGGTPILAPAVLNAPGLGRALLVALCFYSIGVFFLMFSMYMQSALHMSALHAGLIFLPFGAG
FLLGPLSVPLLRRILGGYVNPVGMGLEVVGLLWLGWLVSVTPTTLAPPFTSLTVILFLIGLGQGLGLPTLMRMVTGRVAP
AYAGMIAGITSATLQISTSLSVALIGGVFYTLLGEPATAVSITHAFTIAVLCIAVCLAVGTAISLTLAREPATVAQASAV
RPT

Specific function: Promotes the efflux of actinorhodin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=360, Percent_Identity=24.1666666666667, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI1789042, Length=214, Percent_Identity=28.9719626168224, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788710, Length=344, Percent_Identity=26.7441860465116, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788392, Length=491, Percent_Identity=26.0692464358452, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1790195, Length=160, Percent_Identity=31.875, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=201, Percent_Identity=31.8407960199005, Blast_Score=63, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50947; Mature: 50816

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIEIEVPEPRRWLMFAILLVGAFLPPLDFFIVNVALPSMQEDLGTGSSAEQLVISSYA
CCEEEEECCCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHH
TLYAVTLITGGRLGDLYGRGRMFFLGLLGFAAASLMCGLAVSPWMLILGRALQGVTAAVM
HHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
APQALASVQAIFPVSERPLALSIYGAVFGLASVIGQALGGFLIEIDVLGMGWRAIFLVNL
HHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHH
PIALLVIAFGIRVLKETRAQHASKLDLVGTALSMLTLTTLVVPLIEGREAGWPWWAWLSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
AASPALAVAFWRYESRLHRAGGTPILAPAVLNAPGLGRALLVALCFYSIGVFFLMFSMYM
HCCHHHHHHHHHHHHHHHHCCCCCEECHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QSALHMSALHAGLIFLPFGAGFLLGPLSVPLLRRILGGYVNPVGMGLEVVGLLWLGWLVS
HHHHHHHHHHHCHHEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
VTPTTLAPPFTSLTVILFLIGLGQGLGLPTLMRMVTGRVAPAYAGMIAGITSATLQISTS
CCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEECC
LSVALIGGVFYTLLGEPATAVSITHAFTIAVLCIAVCLAVGTAISLTLAREPATVAQASA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHCCC
VRPT
CCCC
>Mature Secondary Structure 
SRIEIEVPEPRRWLMFAILLVGAFLPPLDFFIVNVALPSMQEDLGTGSSAEQLVISSYA
CEEEEECCCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHH
TLYAVTLITGGRLGDLYGRGRMFFLGLLGFAAASLMCGLAVSPWMLILGRALQGVTAAVM
HHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
APQALASVQAIFPVSERPLALSIYGAVFGLASVIGQALGGFLIEIDVLGMGWRAIFLVNL
HHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHH
PIALLVIAFGIRVLKETRAQHASKLDLVGTALSMLTLTTLVVPLIEGREAGWPWWAWLSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
AASPALAVAFWRYESRLHRAGGTPILAPAVLNAPGLGRALLVALCFYSIGVFFLMFSMYM
HCCHHHHHHHHHHHHHHHHCCCCCEECHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QSALHMSALHAGLIFLPFGAGFLLGPLSVPLLRRILGGYVNPVGMGLEVVGLLWLGWLVS
HHHHHHHHHHHCHHEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
VTPTTLAPPFTSLTVILFLIGLGQGLGLPTLMRMVTGRVAPAYAGMIAGITSATLQISTS
CCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEECC
LSVALIGGVFYTLLGEPATAVSITHAFTIAVLCIAVCLAVGTAISLTLAREPATVAQASA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHCCC
VRPT
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1878971; 12000953 [H]