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The map label for this gene is por [H]

Identifier: 77458861

GI number: 77458861

Start: 3017276

End: 3018748

Strand: Reverse

Name: por [H]

Synonym: Pfl01_2636

Alternate gene names: 77458861

Gene position: 3018748-3017276 (Counterclockwise)

Preceding gene: 77458862

Following gene: 77458860

Centisome position: 46.89

GC content: 61.44

Gene sequence:

>1473_bases
ATGAAACTGAACCAGCAAAACCTCAACCGCCTGGCCGCCGAAGTGAAACTGCCGGCCTACAGCCTCAGCGACACCCGCCA
AGGCATCGCCCACATTGGCGTCGGCGGCTTCCACCGCGCGCATCAGGCGTATTACACCGATGCGCTGATGAACACCGGCG
AAGCACTGGACTGGGCGATTTGCGGCGTTGGCCTGCGCGCCGAAGACCGCCGCGCCCGCGACGATCTCAAGGAGCAGGAC
TACCTGTTCACCCTGTTCGAACTGGGCGACAGCGACGACACCGAAGTCCGGGTCATCGGCGCGATCCGCGACATGCTGCT
GGCCGAGGACAGCGCCCAGGCGCTGATCGACAAACTCGCCGATCCGCAGATCCGCATCGTCTCGCTGACCATCACCGAGG
GCGGTTATTGCATCGACGACAGCAACGGCGAATTCATGGCGCACCTGCCGCAGATTCAGCACGACCTGACGAACCCGCAA
CAGCCGAAAACCGTGTTCGGTTTCCTCTGTGCGGCGTTGGCCAAGCGGCGTGCGGCGGGCACTCCGGCGTTCACGGTGAT
GTCGTGCGATAACCTGCCGCACAACGGCGCGGTGACCCGCAAGGCACTGTTGGCCTTTACTGCTTCGCTGGATCCGGACC
TGCACGACTGGATCGACGCCAACGTCAGCTTCCCCAACGCCATGGTCGATCGCATCACGCCGATGACCAGCATCGCCCAT
CGCCTGCAACTGGCGGACAAGCACGCCGTCGATGACGCCTGGCCGGTGGTCTGCGAACCGTTTGTGCAATGGGTGCTGGA
GGACAAGTTCGTCAACGGCCGCCCGGCCTGGGAAAAGGTCGGCGTGCAGTTCACCGACGATGTTTCCCCCTACGAAGAGA
TGAAGATCAAACTGCTCAACGGTAGCCATCTGGCGCTGACTTACCTGGGCTTTTTGAAGGGTTACCGCTTCGTTCACGAA
ACCATGAACGACCCGCTGTTCGTGCGCTACATGCGCGCCTATATGGACCTGGACGTGACTCCGCAGCTGGCGCCGGTGCC
GGGCATCGACCTCACCGAGTACAAGGACACGCTGGTGGCGCGGTTCTCCAATCAGGCGATTGCCGATCAGCTTGAACGGG
TTTGCTCCGATGGCTCGTCGAAGTTTCCCAAGTTCACCATTCCGACGATCAATCGGTTGATTGCCGATGGGCGCGAGACC
AAACGCGCGGCCTTGGTGGTGGCGGCCTGGGCCCTTTACCTGAAGGGCGTGGATGAGAATGGCGACACTTACTCGGTTCC
GGATCCTCGGGCGGCGTTCTGTCAGGGGTTGGTGGCGGAGGATGCTTTGATCACTGAGCGGTTGTTGGGGGTTGAAGAAA
TCTTCGGTACGGCGATACCGCGTTCGCCAGAGTTTGTTGCGGCGTTTGAGTGGTGTCTGAACAGCTTGCGAGAAGAAGGC
GTCACTCGGACTCTCGAGCGTATTCTGGCGTGA

Upstream 100 bases:

>100_bases
AACCTGCACCTGGACGCCTCCCACTGCCATCTGTTCGATGCCAACGGCGTGGCGCTGACCCGCCCGTTGCGCGCGGCCGC
CTGATTTCGAGACTGACCCG

Downstream 100 bases:

>100_bases
AAAGCCCCTCACCCCAGCCCTCTCCCAGAGGGAGAGGGAGCCGACCGAGGTGTTTATCCGAGGTACATCGACCTGAAAGT
CCGGAGCCGAACTCAGGAAT

Product: mannitol dehydrogenase

Products: D-Fructuronate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: NA

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MKLNQQNLNRLAAEVKLPAYSLSDTRQGIAHIGVGGFHRAHQAYYTDALMNTGEALDWAICGVGLRAEDRRARDDLKEQD
YLFTLFELGDSDDTEVRVIGAIRDMLLAEDSAQALIDKLADPQIRIVSLTITEGGYCIDDSNGEFMAHLPQIQHDLTNPQ
QPKTVFGFLCAALAKRRAAGTPAFTVMSCDNLPHNGAVTRKALLAFTASLDPDLHDWIDANVSFPNAMVDRITPMTSIAH
RLQLADKHAVDDAWPVVCEPFVQWVLEDKFVNGRPAWEKVGVQFTDDVSPYEEMKIKLLNGSHLALTYLGFLKGYRFVHE
TMNDPLFVRYMRAYMDLDVTPQLAPVPGIDLTEYKDTLVARFSNQAIADQLERVCSDGSSKFPKFTIPTINRLIADGRET
KRAALVVAAWALYLKGVDENGDTYSVPDPRAAFCQGLVAEDALITERLLGVEEIFGTAIPRSPEFVAAFEWCLNSLREEG
VTRTLERILA

Sequences:

>Translated_490_residues
MKLNQQNLNRLAAEVKLPAYSLSDTRQGIAHIGVGGFHRAHQAYYTDALMNTGEALDWAICGVGLRAEDRRARDDLKEQD
YLFTLFELGDSDDTEVRVIGAIRDMLLAEDSAQALIDKLADPQIRIVSLTITEGGYCIDDSNGEFMAHLPQIQHDLTNPQ
QPKTVFGFLCAALAKRRAAGTPAFTVMSCDNLPHNGAVTRKALLAFTASLDPDLHDWIDANVSFPNAMVDRITPMTSIAH
RLQLADKHAVDDAWPVVCEPFVQWVLEDKFVNGRPAWEKVGVQFTDDVSPYEEMKIKLLNGSHLALTYLGFLKGYRFVHE
TMNDPLFVRYMRAYMDLDVTPQLAPVPGIDLTEYKDTLVARFSNQAIADQLERVCSDGSSKFPKFTIPTINRLIADGRET
KRAALVVAAWALYLKGVDENGDTYSVPDPRAAFCQGLVAEDALITERLLGVEEIFGTAIPRSPEFVAAFEWCLNSLREEG
VTRTLERILA
>Mature_490_residues
MKLNQQNLNRLAAEVKLPAYSLSDTRQGIAHIGVGGFHRAHQAYYTDALMNTGEALDWAICGVGLRAEDRRARDDLKEQD
YLFTLFELGDSDDTEVRVIGAIRDMLLAEDSAQALIDKLADPQIRIVSLTITEGGYCIDDSNGEFMAHLPQIQHDLTNPQ
QPKTVFGFLCAALAKRRAAGTPAFTVMSCDNLPHNGAVTRKALLAFTASLDPDLHDWIDANVSFPNAMVDRITPMTSIAH
RLQLADKHAVDDAWPVVCEPFVQWVLEDKFVNGRPAWEKVGVQFTDDVSPYEEMKIKLLNGSHLALTYLGFLKGYRFVHE
TMNDPLFVRYMRAYMDLDVTPQLAPVPGIDLTEYKDTLVARFSNQAIADQLERVCSDGSSKFPKFTIPTINRLIADGRET
KRAALVVAAWALYLKGVDENGDTYSVPDPRAAFCQGLVAEDALITERLLGVEEIFGTAIPRSPEFVAAFEWCLNSLREEG
VTRTLERILA

Specific function: Hexuronate conversion to KDG. [C]

COG id: COG0246

COG function: function code G; Mannitol-1-phosphate/altronate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannitol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1790779, Length=470, Percent_Identity=40.8510638297872, Blast_Score=360, Evalue=1e-101,
Organism=Escherichia coli, GI1788497, Length=482, Percent_Identity=40.8713692946058, Blast_Score=360, Evalue=1e-100,
Organism=Escherichia coli, GI1787823, Length=483, Percent_Identity=39.1304347826087, Blast_Score=344, Evalue=7e-96,
Organism=Escherichia coli, GI48994885, Length=410, Percent_Identity=24.390243902439, Blast_Score=96, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6324401, Length=490, Percent_Identity=41.6326530612245, Blast_Score=405, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6320765, Length=490, Percent_Identity=41.6326530612245, Blast_Score=405, Evalue=1e-114,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR000669
- InterPro:   IPR013118
- InterPro:   IPR013131
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01232 Mannitol_dh; PF08125 Mannitol_dh_C [H]

EC number: 1.1.1.57 [C]

Molecular weight: Translated: 54525; Mature: 54525

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00974 MANNITOL_DHGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNQQNLNRLAAEVKLPAYSLSDTRQGIAHIGVGGFHRAHQAYYTDALMNTGEALDWAI
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
CGVGLRAEDRRARDDLKEQDYLFTLFELGDSDDTEVRVIGAIRDMLLAEDSAQALIDKLA
HCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEHHHHHHHHHCCCHHHHHHHHHC
DPQIRIVSLTITEGGYCIDDSNGEFMAHLPQIQHDLTNPQQPKTVFGFLCAALAKRRAAG
CCCEEEEEEEEECCCEEEECCCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
TPAFTVMSCDNLPHNGAVTRKALLAFTASLDPDLHDWIDANVSFPNAMVDRITPMTSIAH
CCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHCHHHHHHH
RLQLADKHAVDDAWPVVCEPFVQWVLEDKFVNGRPAWEKVGVQFTDDVSPYEEMKIKLLN
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCEECCCCCCHHHHEEEEEC
GSHLALTYLGFLKGYRFVHETMNDPLFVRYMRAYMDLDVTPQLAPVPGIDLTEYKDTLVA
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
RFSNQAIADQLERVCSDGSSKFPKFTIPTINRLIADGRETKRAALVVAAWALYLKGVDEN
HHCCHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
GDTYSVPDPRAAFCQGLVAEDALITERLLGVEEIFGTAIPRSPEFVAAFEWCLNSLREEG
CCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
VTRTLERILA
HHHHHHHHHC
>Mature Secondary Structure
MKLNQQNLNRLAAEVKLPAYSLSDTRQGIAHIGVGGFHRAHQAYYTDALMNTGEALDWAI
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
CGVGLRAEDRRARDDLKEQDYLFTLFELGDSDDTEVRVIGAIRDMLLAEDSAQALIDKLA
HCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEHHHHHHHHHCCCHHHHHHHHHC
DPQIRIVSLTITEGGYCIDDSNGEFMAHLPQIQHDLTNPQQPKTVFGFLCAALAKRRAAG
CCCEEEEEEEEECCCEEEECCCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
TPAFTVMSCDNLPHNGAVTRKALLAFTASLDPDLHDWIDANVSFPNAMVDRITPMTSIAH
CCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHCHHHHHHH
RLQLADKHAVDDAWPVVCEPFVQWVLEDKFVNGRPAWEKVGVQFTDDVSPYEEMKIKLLN
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCEECCCCCCHHHHEEEEEC
GSHLALTYLGFLKGYRFVHETMNDPLFVRYMRAYMDLDVTPQLAPVPGIDLTEYKDTLVA
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
RFSNQAIADQLERVCSDGSSKFPKFTIPTINRLIADGRETKRAALVVAAWALYLKGVDEN
HHCCHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
GDTYSVPDPRAAFCQGLVAEDALITERLLGVEEIFGTAIPRSPEFVAAFEWCLNSLREEG
CCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
VTRTLERILA
HHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+; NADH; NADPH [C]

Metal ions: No metal ion requirement [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1 {D-fructuronic} 0.2 {NAD+}} 0.7 {NADPH}} 1.8 {mannonate}} 1.7 {mannonate}} 0.5 {fructuronate}} 0.03 {NADH}} [C]

Substrates: D-Mannonate; Nicotinamide adenine dinucleotide [C]

Specific reaction: D-Mannonate + Nicotinamide adenine dinucleotide <==> D-Fructuronate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: Altronate; ATP; Fructuronate; Mannonate; NAD+; NADH; p-Chloromercuribenzoate; Tagaturonate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA