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The map label for this gene is cmr [H]

Identifier: 77458785

GI number: 77458785

Start: 2933299

End: 2934546

Strand: Reverse

Name: cmr [H]

Synonym: Pfl01_2560

Alternate gene names: 77458785

Gene position: 2934546-2933299 (Counterclockwise)

Preceding gene: 77458787

Following gene: 77458784

Centisome position: 45.58

GC content: 61.86

Gene sequence:

>1248_bases
ATGCAAAGAACTCTCATCCCCCTCCCGTCACTCCAGGCGCTCGGTTTCTGTCTCGCCATCACGCTGTTCGAATGGCTGAC
CTACATGGCCAGTGACATGGTCATGCCGGCCATGCTGACCGTCACCCGGGATCTGGAAGCCGATGCGGCTCACATCCCCA
ACGCGTTCAATCTGTATCTGATCGGCGGCGTCTGCCTGCAATGGCTGATCGGGCCTCTGTCCGACCGGTTTGGTCGGCGC
CCGCTATTACTCGGCGGTTGCGCGCTGTTCGGCGTGACCTGTGCGGCGGCTGTCGCCACGCCCGGCATCGAAGTGTTCAA
CGCCCTGCGACTGATGCAAGGCATGGGTTTGGGGTTTGTCGTGGCAGTCAGCTACCCCGCGCTGCAGGAAGTTTTCTGCG
AAGCTGATGCGGTGCGTCTGATGGCGCTGCTGAGCAATGTCGCGTTGCTTTCACCTCTGCTCGGTCCGCTGCTTGGCAGT
CTGTTGCTGGAATGGTTGAGCTGGCGCGAGCTGTTCCTCGGTCTGGGCCTGATCGCGACACTGACCTGGATCGGACTGTA
TGCCTGTATGCCGGAGACGATCGGCGTTGTGCGCCACGATGGCGGTCAGCAAGCGCCGATCCCCTTCTCCTTTCGCCAGA
TCAGCAGCCGCTACCTGTTACTGCTGCGCAATCACCGCTTCGTCGGCATCTGTCTTGCCCTGGGGCTGATGAGCCTGCCG
CTGATTGCCTGGATCGGAATGTCGCCGCTGCTGCTCGTCCAGTCGCTCGGCCTGACTACTCGCGAATATGCGCTATGGCA
GATTCCGGTTTTTTCGGCGGTCATCGTCGGCAATCTGATCCTCGACAGACTGCTCGCGTCACGGGAACTGCCGCAACTGA
TTCGTCTGGCACTTTGGCCGTTCTGCCTGGGTCTGCTGACCCTGATCGCCACCGCACTGTCCAGCCTGTCCCTTGCAGGG
TTGATGACTGGCCTTGCGCTGTACGCCGTGGGGCTGGGCATGAGCAACGCTGCACTGTACCGGCTCGCGCTGTTTTCCAG
CGATGACAGCAAAGGTCTGGTCTCCGCCACGATCGGCATGATTTCGATTGCCGTGATGGGTGTCGGCGGCTCGCTGATCG
CTGCCATCGGCGGCGGTACCCGTCTCGAATCCTTCGCCATGTGGGCGGCGCTGGGCGGTCTGCTCAGCCTGCCCCTGCTG
TACCGGTTCCTGCCACACGTCCCGGCAGACTCCGCTTCTTCAACATAA

Upstream 100 bases:

>100_bases
ATCACGCATAACCGCGTCATAGACCGTCACACCAATGGCGTAGGAACATTCCCTGCTCCTCCCTACCCTTTGCGCTCCCT
CTCGACGATCGTGAAAGCGC

Downstream 100 bases:

>100_bases
CGATAAGGACCCCTGATGAGTCACTTCCCCCACAGCGACGCGCTTTGCGCGCTGACGCAACCCTATTGCCCGGATTCCGT
GCCGGACGGCCTGTTTGACC

Product: major facilitator transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Chloramphenicol resistance pump cmr [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MQRTLIPLPSLQALGFCLAITLFEWLTYMASDMVMPAMLTVTRDLEADAAHIPNAFNLYLIGGVCLQWLIGPLSDRFGRR
PLLLGGCALFGVTCAAAVATPGIEVFNALRLMQGMGLGFVVAVSYPALQEVFCEADAVRLMALLSNVALLSPLLGPLLGS
LLLEWLSWRELFLGLGLIATLTWIGLYACMPETIGVVRHDGGQQAPIPFSFRQISSRYLLLLRNHRFVGICLALGLMSLP
LIAWIGMSPLLLVQSLGLTTREYALWQIPVFSAVIVGNLILDRLLASRELPQLIRLALWPFCLGLLTLIATALSSLSLAG
LMTGLALYAVGLGMSNAALYRLALFSSDDSKGLVSATIGMISIAVMGVGGSLIAAIGGGTRLESFAMWAALGGLLSLPLL
YRFLPHVPADSASST

Sequences:

>Translated_415_residues
MQRTLIPLPSLQALGFCLAITLFEWLTYMASDMVMPAMLTVTRDLEADAAHIPNAFNLYLIGGVCLQWLIGPLSDRFGRR
PLLLGGCALFGVTCAAAVATPGIEVFNALRLMQGMGLGFVVAVSYPALQEVFCEADAVRLMALLSNVALLSPLLGPLLGS
LLLEWLSWRELFLGLGLIATLTWIGLYACMPETIGVVRHDGGQQAPIPFSFRQISSRYLLLLRNHRFVGICLALGLMSLP
LIAWIGMSPLLLVQSLGLTTREYALWQIPVFSAVIVGNLILDRLLASRELPQLIRLALWPFCLGLLTLIATALSSLSLAG
LMTGLALYAVGLGMSNAALYRLALFSSDDSKGLVSATIGMISIAVMGVGGSLIAAIGGGTRLESFAMWAALGGLLSLPLL
YRFLPHVPADSASST
>Mature_415_residues
MQRTLIPLPSLQALGFCLAITLFEWLTYMASDMVMPAMLTVTRDLEADAAHIPNAFNLYLIGGVCLQWLIGPLSDRFGRR
PLLLGGCALFGVTCAAAVATPGIEVFNALRLMQGMGLGFVVAVSYPALQEVFCEADAVRLMALLSNVALLSPLLGPLLGS
LLLEWLSWRELFLGLGLIATLTWIGLYACMPETIGVVRHDGGQQAPIPFSFRQISSRYLLLLRNHRFVGICLALGLMSLP
LIAWIGMSPLLLVQSLGLTTREYALWQIPVFSAVIVGNLILDRLLASRELPQLIRLALWPFCLGLLTLIATALSSLSLAG
LMTGLALYAVGLGMSNAALYRLALFSSDDSKGLVSATIGMISIAVMGVGGSLIAAIGGGTRLESFAMWAALGGLLSLPLL
YRFLPHVPADSASST

Specific function: Efflux pump driven by the proton motive force. Confers resistance to basic, aromatic and lipophilic drugs as well as neutral, non-aromatic, zwitterionic or non-aromatic hydrophilic antibiotics [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787065, Length=398, Percent_Identity=42.2110552763819, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1790794, Length=356, Percent_Identity=34.5505617977528, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI48994889, Length=316, Percent_Identity=26.8987341772152, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1788509, Length=343, Percent_Identity=20.9912536443149, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790146, Length=236, Percent_Identity=26.271186440678, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI87082312, Length=165, Percent_Identity=32.1212121212121, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44333; Mature: 44333

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRTLIPLPSLQALGFCLAITLFEWLTYMASDMVMPAMLTVTRDLEADAAHIPNAFNLYL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHH
IGGVCLQWLIGPLSDRFGRRPLLLGGCALFGVTCAAAVATPGIEVFNALRLMQGMGLGFV
HHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEE
VAVSYPALQEVFCEADAVRLMALLSNVALLSPLLGPLLGSLLLEWLSWRELFLGLGLIAT
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTWIGLYACMPETIGVVRHDGGQQAPIPFSFRQISSRYLLLLRNHRFVGICLALGLMSLP
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LIAWIGMSPLLLVQSLGLTTREYALWQIPVFSAVIVGNLILDRLLASRELPQLIRLALWP
HHHHHCCCHHHHHHHCCCCHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FCLGLLTLIATALSSLSLAGLMTGLALYAVGLGMSNAALYRLALFSSDDSKGLVSATIGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHH
ISIAVMGVGGSLIAAIGGGTRLESFAMWAALGGLLSLPLLYRFLPHVPADSASST
HHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MQRTLIPLPSLQALGFCLAITLFEWLTYMASDMVMPAMLTVTRDLEADAAHIPNAFNLYL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHH
IGGVCLQWLIGPLSDRFGRRPLLLGGCALFGVTCAAAVATPGIEVFNALRLMQGMGLGFV
HHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEE
VAVSYPALQEVFCEADAVRLMALLSNVALLSPLLGPLLGSLLLEWLSWRELFLGLGLIAT
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTWIGLYACMPETIGVVRHDGGQQAPIPFSFRQISSRYLLLLRNHRFVGICLALGLMSLP
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LIAWIGMSPLLLVQSLGLTTREYALWQIPVFSAVIVGNLILDRLLASRELPQLIRLALWP
HHHHHCCCHHHHHHHCCCCHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FCLGLLTLIATALSSLSLAGLMTGLALYAVGLGMSNAALYRLALFSSDDSKGLVSATIGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHH
ISIAVMGVGGSLIAAIGGGTRLESFAMWAALGGLLSLPLLYRFLPHVPADSASST
HHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]