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The map label for this gene is nodW [H]

Identifier: 77458684

GI number: 77458684

Start: 2812627

End: 2813274

Strand: Reverse

Name: nodW [H]

Synonym: Pfl01_2459

Alternate gene names: 77458684

Gene position: 2813274-2812627 (Counterclockwise)

Preceding gene: 77458686

Following gene: 77458683

Centisome position: 43.7

GC content: 61.88

Gene sequence:

>648_bases
ATGAACAACAATCCCATCAGCAACAACCCCAGCGAGCCGGTGGTATTCATCGTCGACGACGATGCCGCGTTGCGCGAATC
CCTCGGCAGCCTGTTGCGCTCCATTGGTCTGCGGGTGGAGCTATTCGGATCGGTCGCCGAGTTCATGACGTATCAACGTC
CGGACACGCTGAGTTGCCTGGTACTCGACGTGCGCCTGCAAGGCAGCAGCGGGCTGGATTTCCAGAATGAACTGGCCACC
GCCGGAATCAATGTGCCAATCGTGTTCATCACCGGACACGGCGATATCGCCATGACCGTGCGCGCGATGAAAGCCGGCGC
AGTGGACTTCCTGACCAAACCGTTTCGCGAGCAGGACCTGATCGATGCCGTCTGCGCCGCACACTCGCGGGACAAGCAGC
GTCGCGAGTCAGGGCGGCATGCCGATGAACTGCGCGCCCGTCATGGGACGTTGACACCCCGGGAGCAGACGGTCATGGCG
TTGGCAGCCTCAGGCTTGATGAACAAGCAGATCGCCGGGGAAATCGGCCTCAGTGAAATCACTGTGAAGATCCACCGGGG
CCAGGCCATGCGCAAGATGGGCGCGCGCTCATTTGCCGACCTCGTGCGCATGGCCGAAGTCATCGCGGCGCTGCCTGCAC
ATCGGTGA

Upstream 100 bases:

>100_bases
CGGGACCGTGTCTCTGGTCGGGCATCAACCCGCACGAGCAAACACCGGCCGCCCGGCCAAGGGATTCGCCACAACGTCAC
AACCTTTTGGGAAAAGAGCA

Downstream 100 bases:

>100_bases
CCGAATGAAACGATACGGTATGGCTCAAGCCGCCGTGGCTGCCCGGCACAATCAACACTCAAGTTCCGAACAACTAACAC
TTGGGTATATCCATTTCATA

Product: two component LuxR family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MNNNPISNNPSEPVVFIVDDDAALRESLGSLLRSIGLRVELFGSVAEFMTYQRPDTLSCLVLDVRLQGSSGLDFQNELAT
AGINVPIVFITGHGDIAMTVRAMKAGAVDFLTKPFREQDLIDAVCAAHSRDKQRRESGRHADELRARHGTLTPREQTVMA
LAASGLMNKQIAGEIGLSEITVKIHRGQAMRKMGARSFADLVRMAEVIAALPAHR

Sequences:

>Translated_215_residues
MNNNPISNNPSEPVVFIVDDDAALRESLGSLLRSIGLRVELFGSVAEFMTYQRPDTLSCLVLDVRLQGSSGLDFQNELAT
AGINVPIVFITGHGDIAMTVRAMKAGAVDFLTKPFREQDLIDAVCAAHSRDKQRRESGRHADELRARHGTLTPREQTVMA
LAASGLMNKQIAGEIGLSEITVKIHRGQAMRKMGARSFADLVRMAEVIAALPAHR
>Mature_215_residues
MNNNPISNNPSEPVVFIVDDDAALRESLGSLLRSIGLRVELFGSVAEFMTYQRPDTLSCLVLDVRLQGSSGLDFQNELAT
AGINVPIVFITGHGDIAMTVRAMKAGAVDFLTKPFREQDLIDAVCAAHSRDKQRRESGRHADELRARHGTLTPREQTVMA
LAASGLMNKQIAGEIGLSEITVKIHRGQAMRKMGARSFADLVRMAEVIAALPAHR

Specific function: Member of the two-component regulatory system nodV/nodW probably involved in the regulation of the transcription of genes involved in the nodulation process [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=126, Percent_Identity=30.1587301587302, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1786747, Length=204, Percent_Identity=24.0196078431373, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1788521, Length=150, Percent_Identity=32.6666666666667, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1790102, Length=201, Percent_Identity=28.8557213930348, Blast_Score=61, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR011990
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23419; Mature: 23419

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNNPISNNPSEPVVFIVDDDAALRESLGSLLRSIGLRVELFGSVAEFMTYQRPDTLSCL
CCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEE
VLDVRLQGSSGLDFQNELATAGINVPIVFITGHGDIAMTVRAMKAGAVDFLTKPFREQDL
EEEEEECCCCCCCHHHHHHHCCCCEEEEEEECCCCEEEHHHHHHCCHHHHHHCCCCHHHH
IDAVCAAHSRDKQRRESGRHADELRARHGTLTPREQTVMALAASGLMNKQIAGEIGLSEI
HHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
TVKIHRGQAMRKMGARSFADLVRMAEVIAALPAHR
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNNNPISNNPSEPVVFIVDDDAALRESLGSLLRSIGLRVELFGSVAEFMTYQRPDTLSCL
CCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEE
VLDVRLQGSSGLDFQNELATAGINVPIVFITGHGDIAMTVRAMKAGAVDFLTKPFREQDL
EEEEEECCCCCCCHHHHHHHCCCCEEEEEEECCCCEEEHHHHHHCCHHHHHHCCCCHHHH
IDAVCAAHSRDKQRRESGRHADELRARHGTLTPREQTVMALAASGLMNKQIAGEIGLSEI
HHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
TVKIHRGQAMRKMGARSFADLVRMAEVIAALPAHR
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2320582; 11157954; 12597275 [H]