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The map label for this gene is nodW [H]

Identifier: 77458680

GI number: 77458680

Start: 2809027

End: 2809401

Strand: Reverse

Name: nodW [H]

Synonym: Pfl01_2455

Alternate gene names: 77458680

Gene position: 2809401-2809027 (Counterclockwise)

Preceding gene: 77458681

Following gene: 77458678

Centisome position: 43.64

GC content: 55.2

Gene sequence:

>375_bases
ATGTTCCACTCAGCTATTGTTTCAGTTGTCGATGATGACGAACTTGTTCTGGTTTCAATGGACAGTTTGTTGCGGTCTTG
CGGTTATACGGTCAAGACATTCAACAGCGCTTTTGCATTTCTTGAATCGAGCGCGCCAGAGCAGACCGGGTGCCTGATCT
CGGACATTCAGATGCCCGGCATGTGCGGGGTCGAAATGTACGAGGCGCTGGCGGCCAGGAAGATCCATATTCCGACGATT
TTCATCACTGGCAAGCCGGGCCTGCCCCCCGAGTTCAGTGCGCGGGTGAAGACGCCGGAAGGTTATTTCTCCAAACCGTT
CCATACCGGGGCATTGCTGGCCTGCATCGAACAGGCGCTGATGCGCCGCCGTTGA

Upstream 100 bases:

>100_bases
CAACTAAACATACCCACGTATACTATTCCACAATTTTTGAAAGCGTATCTTGCAAGTATGGCGGGCGCGACAGACACCCG
CAAAGAACGCAAGGTTTCCC

Downstream 100 bases:

>100_bases
GCGGCCCTGACGAATAACTGGATGCAGAGCCGCCGCTGCCCGACCAACAAACCTCAAAGCAAAAAAAGGCCCCGCAAGCT
CATCACTTGCGGGGCCTGAT

Product: response regulator receiver domain-containing protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 124; Mature: 124

Protein sequence:

>124_residues
MFHSAIVSVVDDDELVLVSMDSLLRSCGYTVKTFNSAFAFLESSAPEQTGCLISDIQMPGMCGVEMYEALAARKIHIPTI
FITGKPGLPPEFSARVKTPEGYFSKPFHTGALLACIEQALMRRR

Sequences:

>Translated_124_residues
MFHSAIVSVVDDDELVLVSMDSLLRSCGYTVKTFNSAFAFLESSAPEQTGCLISDIQMPGMCGVEMYEALAARKIHIPTI
FITGKPGLPPEFSARVKTPEGYFSKPFHTGALLACIEQALMRRR
>Mature_124_residues
MFHSAIVSVVDDDELVLVSMDSLLRSCGYTVKTFNSAFAFLESSAPEQTGCLISDIQMPGMCGVEMYEALAARKIHIPTI
FITGKPGLPPEFSARVKTPEGYFSKPFHTGALLACIEQALMRRR

Specific function: Member of the two-component regulatory system nodV/nodW probably involved in the regulation of the transcription of genes involved in the nodulation process [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=116, Percent_Identity=32.7586206896552, Blast_Score=65, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR011990
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 13605; Mature: 13605

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
8.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFHSAIVSVVDDDELVLVSMDSLLRSCGYTVKTFNSAFAFLESSAPEQTGCLISDIQMPG
CCCHHEEEEECCCCEEEEEHHHHHHHCCCEEHHHHHHHHHHHCCCCCCCCCEEECCCCCC
MCGVEMYEALAARKIHIPTIFITGKPGLPPEFSARVKTPEGYFSKPFHTGALLACIEQAL
CCCHHHHHHHHHCEECCCEEEEECCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHH
MRRR
HHCC
>Mature Secondary Structure
MFHSAIVSVVDDDELVLVSMDSLLRSCGYTVKTFNSAFAFLESSAPEQTGCLISDIQMPG
CCCHHEEEEECCCCEEEEEHHHHHHHCCCEEHHHHHHHHHHHCCCCCCCCCEEECCCCCC
MCGVEMYEALAARKIHIPTIFITGKPGLPPEFSARVKTPEGYFSKPFHTGALLACIEQAL
CCCHHHHHHHHHCEECCCEEEEECCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHH
MRRR
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2320582; 11157954; 12597275 [H]