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The map label for this gene is fixJ [H]

Identifier: 77458677

GI number: 77458677

Start: 2806105

End: 2806527

Strand: Reverse

Name: fixJ [H]

Synonym: Pfl01_2452

Alternate gene names: 77458677

Gene position: 2806527-2806105 (Counterclockwise)

Preceding gene: 77458678

Following gene: 77458676

Centisome position: 43.59

GC content: 61.94

Gene sequence:

>423_bases
ATGGCGGGCGTCCTCGACGGGCTACCCGAGATCAGCCCTGGTAAAGCAATGTCCCACACCGCTGTTGTATCGGTTGTCGA
TGATGATGAGTCCGTGCGCATGGCGCTGGACAGCCTGTTGCGCTCGCACGGCTACACCGTGCAGTTGTACCCGAACGCGG
AGGCTTTTCTGGCGATGAAGGCGCCGCCCCGCCCCGGCTGCATCATCTCGGACATCCAGATGCCCGGTCTGTCCGGCATC
GAGATGTACGATGAGCTGTGCGCCCGAGGCGTGCGCATCCCGATCATTTTCATCACGGGTTACCAGGGGCGTCCGCCAAG
GGTCAACGCCGCATTCCCCGAGCCCCTCGCCTATTTCCCTAAACCCTTCCCGTGCGGCGAACTCATCACCTGCATCGAAA
GCGCATTCAATCGCGCCGTTTGA

Upstream 100 bases:

>100_bases
CGCCACACAGCGCAATGGCACCGGGCTCGCAGACGTTCAAATACTGTCGCTATACACACGTATAAGTACGCCGCCGAACC
GAGCGGCGTATCGTGCGGCA

Downstream 100 bases:

>100_bases
CGGCCCTCTTTCTATACCTGTGTATACCCCACCCCATACCTAAGCATGTCGGCACTGACCCTATGTAGAAGTGTCTCGCC
GCACCTTCCCCGATAGCATC

Product: response regulator receiver domain-containing protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 140; Mature: 139

Protein sequence:

>140_residues
MAGVLDGLPEISPGKAMSHTAVVSVVDDDESVRMALDSLLRSHGYTVQLYPNAEAFLAMKAPPRPGCIISDIQMPGLSGI
EMYDELCARGVRIPIIFITGYQGRPPRVNAAFPEPLAYFPKPFPCGELITCIESAFNRAV

Sequences:

>Translated_140_residues
MAGVLDGLPEISPGKAMSHTAVVSVVDDDESVRMALDSLLRSHGYTVQLYPNAEAFLAMKAPPRPGCIISDIQMPGLSGI
EMYDELCARGVRIPIIFITGYQGRPPRVNAAFPEPLAYFPKPFPCGELITCIESAFNRAV
>Mature_139_residues
AGVLDGLPEISPGKAMSHTAVVSVVDDDESVRMALDSLLRSHGYTVQLYPNAEAFLAMKAPPRPGCIISDIQMPGLSGIE
MYDELCARGVRIPIIFITGYQGRPPRVNAAFPEPLAYFPKPFPCGELITCIESAFNRAV

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=117, Percent_Identity=31.6239316239316, Blast_Score=71, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 15139; Mature: 15008

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGVLDGLPEISPGKAMSHTAVVSVVDDDESVRMALDSLLRSHGYTVQLYPNAEAFLAMK
CCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCEEEEEC
APPRPGCIISDIQMPGLSGIEMYDELCARGVRIPIIFITGYQGRPPRVNAAFPEPLAYFP
CCCCCCCEEECCCCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCHHHCC
KPFPCGELITCIESAFNRAV
CCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AGVLDGLPEISPGKAMSHTAVVSVVDDDESVRMALDSLLRSHGYTVQLYPNAEAFLAMK
CCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCEEEEEC
APPRPGCIISDIQMPGLSGIEMYDELCARGVRIPIIFITGYQGRPPRVNAAFPEPLAYFP
CCCCCCCEEECCCCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCHHHCC
KPFPCGELITCIESAFNRAV
CCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2046550 [H]