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The map label for this gene is nemA [H]

Identifier: 77458670

GI number: 77458670

Start: 2796804

End: 2797919

Strand: Reverse

Name: nemA [H]

Synonym: Pfl01_2445

Alternate gene names: 77458670

Gene position: 2797919-2796804 (Counterclockwise)

Preceding gene: 77458671

Following gene: 77458667

Centisome position: 43.46

GC content: 64.61

Gene sequence:

>1116_bases
ATGACACAACAACTGTTCCGTCCCATCGCCCTCGGCCCCTACACACTTCCCCACCGGGTGGCGATGGCGCCGTTGACCCG
CTCCCGTGCCGGACAACCGGGCGACATCCCGACGTCCATGAACGCCGAGTACTACCGCCAGCGGGCGAGCGCCGCGCTGA
TCATCACCGAGGCCACGCAGATTTCCCGCCAGGGCCAGGGTTATGCGTGGACGCCTGGCATCTACAACGATGAACAAGTG
CAAGCCTGGCGTGAAGTCAGCCGCCAGGTACACGAGGCCGGCGGGTTGATCTTCATGCAACTGTGGCATGTGGGCCGCGT
GTCGCACCCAAGCTTTCAACCGGACAACGCACTGCCGGTGGCGCCTAGCGCATTGCCTGTGCCGGGCAAGACCTTCATCG
TCGACGACGAGGGCAACGGTGTCTGGGGCGACGTGCCGGTGCCGCGCGCACTGCAAACGTCGGAGATCGCCGACATCATC
AGCGACTACCGCCGCGCTGCGCGCAATGCACTGAACGCCGGGATGGATGGCGTGGAAATTCACGCCGGCAACGGCTATCT
GCTGGACCAGTTCATCAACAGCAACAGCAACCAGCGCACCGACGGTTATGGCGGCCCCCTGGAAAACCGTGCGCGCCTGT
TACTGGAAGTGGTGGCGGCCGTGGTCGACGAAGTCGGTGCCGACCGTGTCGGCGTGCGCCTGACACCCATGGGCCGCTTC
ATGGGCATGGGCGATGAATCGCCGGAGGCCACATTCGGCTACATCGTGCGCTCGTTGAACCGCTGGAAGCTGGCCTACCT
GCACTTGGTCGAGCCGGCCGTGGTCGGCACTGTCAAGGATGAAAACTTCGATCCGCGCTGGGACGCGATCATCGCTCAGT
TGCGTGATGCCTGGGATGGCGTGCTGATGATTGCCGGCGGCTACGACCCCGAGTCGGCGGAACAGGCCCTGGCCGACGGT
CGCGCCGACATTATCGCGTTTGGCCGACCGTTCCTCGCCAACCCCGATCTGCCTCGGCGAATCCGCGAAGGCCTGACGCT
CAACGCACCGGACCCGAGCACGTTCTTTGGCGGCGATCAGCGTGGCTATACCGACTACCCGGTTTACGCCCACTGA

Upstream 100 bases:

>100_bases
TATCAAGCACCGGACAGCCGCTTAACGTAGGGCCATCCGGTGTCGGGCCAAATGTCCCGACACCGCCCTGCCGATCCTTT
TCGAAGACTGGAGAACCCCC

Downstream 100 bases:

>100_bases
TACGCTGAACAGCGGACGCAAAAAGGCCTGCCACATCGGCAGGCCTTCTTCGTTTTCGCGGAACGTTACTGCTTGAGAAT
CAGCAACGGCTCGCCCTTCT

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MTQQLFRPIALGPYTLPHRVAMAPLTRSRAGQPGDIPTSMNAEYYRQRASAALIITEATQISRQGQGYAWTPGIYNDEQV
QAWREVSRQVHEAGGLIFMQLWHVGRVSHPSFQPDNALPVAPSALPVPGKTFIVDDEGNGVWGDVPVPRALQTSEIADII
SDYRRAARNALNAGMDGVEIHAGNGYLLDQFINSNSNQRTDGYGGPLENRARLLLEVVAAVVDEVGADRVGVRLTPMGRF
MGMGDESPEATFGYIVRSLNRWKLAYLHLVEPAVVGTVKDENFDPRWDAIIAQLRDAWDGVLMIAGGYDPESAEQALADG
RADIIAFGRPFLANPDLPRRIREGLTLNAPDPSTFFGGDQRGYTDYPVYAH

Sequences:

>Translated_371_residues
MTQQLFRPIALGPYTLPHRVAMAPLTRSRAGQPGDIPTSMNAEYYRQRASAALIITEATQISRQGQGYAWTPGIYNDEQV
QAWREVSRQVHEAGGLIFMQLWHVGRVSHPSFQPDNALPVAPSALPVPGKTFIVDDEGNGVWGDVPVPRALQTSEIADII
SDYRRAARNALNAGMDGVEIHAGNGYLLDQFINSNSNQRTDGYGGPLENRARLLLEVVAAVVDEVGADRVGVRLTPMGRF
MGMGDESPEATFGYIVRSLNRWKLAYLHLVEPAVVGTVKDENFDPRWDAIIAQLRDAWDGVLMIAGGYDPESAEQALADG
RADIIAFGRPFLANPDLPRRIREGLTLNAPDPSTFFGGDQRGYTDYPVYAH
>Mature_370_residues
TQQLFRPIALGPYTLPHRVAMAPLTRSRAGQPGDIPTSMNAEYYRQRASAALIITEATQISRQGQGYAWTPGIYNDEQVQ
AWREVSRQVHEAGGLIFMQLWHVGRVSHPSFQPDNALPVAPSALPVPGKTFIVDDEGNGVWGDVPVPRALQTSEIADIIS
DYRRAARNALNAGMDGVEIHAGNGYLLDQFINSNSNQRTDGYGGPLENRARLLLEVVAAVVDEVGADRVGVRLTPMGRFM
GMGDESPEATFGYIVRSLNRWKLAYLHLVEPAVVGTVKDENFDPRWDAIIAQLRDAWDGVLMIAGGYDPESAEQALADGR
ADIIAFGRPFLANPDLPRRIREGLTLNAPDPSTFFGGDQRGYTDYPVYAH

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=369, Percent_Identity=46.6124661246612, Blast_Score=332, Evalue=2e-92,
Organism=Escherichia coli, GI1789463, Length=367, Percent_Identity=30.5177111716621, Blast_Score=122, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17559802, Length=323, Percent_Identity=27.8637770897833, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17565138, Length=299, Percent_Identity=29.0969899665552, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17559804, Length=289, Percent_Identity=29.4117647058824, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI72001454, Length=279, Percent_Identity=30.1075268817204, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17564188, Length=291, Percent_Identity=27.4914089347079, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17540738, Length=274, Percent_Identity=26.2773722627737, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6325086, Length=379, Percent_Identity=39.8416886543536, Blast_Score=248, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6321973, Length=379, Percent_Identity=38.2585751978892, Blast_Score=238, Evalue=2e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40740; Mature: 40609

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQLFRPIALGPYTLPHRVAMAPLTRSRAGQPGDIPTSMNAEYYRQRASAALIITEATQ
CCHHHHCCHHCCCCCCCHHHHHCCHHHHCCCCCCCCCCCCCHHHHHHHCCEEEEEEEHHH
ISRQGQGYAWTPGIYNDEQVQAWREVSRQVHEAGGLIFMQLWHVGRVSHPSFQPDNALPV
HHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEHHHHHCCCCCCCCCCCCCCCC
APSALPVPGKTFIVDDEGNGVWGDVPVPRALQTSEIADIISDYRRAARNALNAGMDGVEI
CCCCCCCCCCEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
HAGNGYLLDQFINSNSNQRTDGYGGPLENRARLLLEVVAAVVDEVGADRVGVRLTPMGRF
ECCCCEEHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHH
MGMGDESPEATFGYIVRSLNRWKLAYLHLVEPAVVGTVKDENFDPRWDAIIAQLRDAWDG
HCCCCCCCCHHHHHHHHHHCHHHEEEHEECCHHHEEEECCCCCCHHHHHHHHHHHHCCCC
VLMIAGGYDPESAEQALADGRADIIAFGRPFLANPDLPRRIREGLTLNAPDPSTFFGGDQ
EEEEECCCCCHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHCCCEECCCCCCCCCCCCC
RGYTDYPVYAH
CCCCCCCCCCC
>Mature Secondary Structure 
TQQLFRPIALGPYTLPHRVAMAPLTRSRAGQPGDIPTSMNAEYYRQRASAALIITEATQ
CHHHHCCHHCCCCCCCHHHHHCCHHHHCCCCCCCCCCCCCHHHHHHHCCEEEEEEEHHH
ISRQGQGYAWTPGIYNDEQVQAWREVSRQVHEAGGLIFMQLWHVGRVSHPSFQPDNALPV
HHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEHHHHHCCCCCCCCCCCCCCCC
APSALPVPGKTFIVDDEGNGVWGDVPVPRALQTSEIADIISDYRRAARNALNAGMDGVEI
CCCCCCCCCCEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
HAGNGYLLDQFINSNSNQRTDGYGGPLENRARLLLEVVAAVVDEVGADRVGVRLTPMGRF
ECCCCEEHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHH
MGMGDESPEATFGYIVRSLNRWKLAYLHLVEPAVVGTVKDENFDPRWDAIIAQLRDAWDG
HCCCCCCCCHHHHHHHHHHCHHHEEEHEECCHHHEEEECCCCCCHHHHHHHHHHHHCCCC
VLMIAGGYDPESAEQALADGRADIIAFGRPFLANPDLPRRIREGLTLNAPDPSTFFGGDQ
EEEEECCCCCHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHCCCEECCCCCCCCCCCCC
RGYTDYPVYAH
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]