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The map label for this gene is gcvA [H]

Identifier: 77458596

GI number: 77458596

Start: 2727674

End: 2728534

Strand: Reverse

Name: gcvA [H]

Synonym: Pfl01_2369

Alternate gene names: 77458596

Gene position: 2728534-2727674 (Counterclockwise)

Preceding gene: 77458601

Following gene: 77458594

Centisome position: 42.38

GC content: 63.88

Gene sequence:

>861_bases
ATGCTGAAACACTGGCCACCCCTCAGCTCGTTGCGCGGCTTCGAAGCCGCCGCAAGGCTGGGCAGTTTTCACAAGGCCGC
CGAGGAACTGAGCCTCACGCAATCGGCCATCAGCCAGCAGATCCGCAGCCTTGAGGCGTACCTCGAGCAACCGCTGTTTT
TCCGCAGCGGACGCAGCGTCAGCCTGACCGACGCCGGCCACGATCTGCTCAGCACCACCCAGGCCATGCTGCAGCAACTG
TCGGTGGGCATCCGGCGGCTGGGGCAATACCAGAAACCCAATCAACTGGTGCTCAACACCACGCCGGCGTTTGCCCGGCA
CTGGCTGCTGCCGCGCCTGGGGGATTTTCGGCGACAGCATCCGCAGGTCGATCTGTGGATTTTCAGCACCGATGAGGTGC
CAGACATGGCGACCCAGACCATCGATCTGGCGGTGCGCGACGACATCAGTTCCCAGGCTGAATGCAGCTTCAAGGTGTTG
CACGCTGACCGCCTCTATCCAGCGTGCCACCCGAGCCTGCTGAGCGTGCCGAGCGCGCAGCGGACGACGCTGCACGGCGA
GCGGGAAATGGACTGGAGCCATTGGGCGGTTGAATCAGGGATCGATGTCGGCCAGCAGGATCAGGGTTTGAATTTCTCTG
ATCCGGGCCTGCTGCTGGACGCTGCATGCGGCGGGCTCGGCATCGCGCTGGTCAGCCAGTTGCTCAGCCGCAAGGCTCGG
GCTGACGGGCTGTTGCAGCCCTTGGTGGACGAGACCATTCGCGGCCCGAACTGGGCGCTGCTGACCCACCGCGACAGCGA
AAACGATCCGATGGCCCGCAGTTTTACCGAATGGCTGCTGGCGAACCTGAGCGATCCATGA

Upstream 100 bases:

>100_bases
GAAAACAGCCTCGGCGGCGTTGACGTGCATGCTAATGGCGGCCTGAAGGGCAAACAAACGATGGATTTAGCGGAAAACTG
ATTAGAGGAACTTATCGATC

Downstream 100 bases:

>100_bases
CCGGTGCGTGGCGGGCCATTACCTCGGCGATCCAGTCGATGAACACCCGCAGCTTGCGGCTGATGTGGCGGTTCGGCGGG
TAGGCGACATAGAGCGGCAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MLKHWPPLSSLRGFEAAARLGSFHKAAEELSLTQSAISQQIRSLEAYLEQPLFFRSGRSVSLTDAGHDLLSTTQAMLQQL
SVGIRRLGQYQKPNQLVLNTTPAFARHWLLPRLGDFRRQHPQVDLWIFSTDEVPDMATQTIDLAVRDDISSQAECSFKVL
HADRLYPACHPSLLSVPSAQRTTLHGEREMDWSHWAVESGIDVGQQDQGLNFSDPGLLLDAACGGLGIALVSQLLSRKAR
ADGLLQPLVDETIRGPNWALLTHRDSENDPMARSFTEWLLANLSDP

Sequences:

>Translated_286_residues
MLKHWPPLSSLRGFEAAARLGSFHKAAEELSLTQSAISQQIRSLEAYLEQPLFFRSGRSVSLTDAGHDLLSTTQAMLQQL
SVGIRRLGQYQKPNQLVLNTTPAFARHWLLPRLGDFRRQHPQVDLWIFSTDEVPDMATQTIDLAVRDDISSQAECSFKVL
HADRLYPACHPSLLSVPSAQRTTLHGEREMDWSHWAVESGIDVGQQDQGLNFSDPGLLLDAACGGLGIALVSQLLSRKAR
ADGLLQPLVDETIRGPNWALLTHRDSENDPMARSFTEWLLANLSDP
>Mature_286_residues
MLKHWPPLSSLRGFEAAARLGSFHKAAEELSLTQSAISQQIRSLEAYLEQPLFFRSGRSVSLTDAGHDLLSTTQAMLQQL
SVGIRRLGQYQKPNQLVLNTTPAFARHWLLPRLGDFRRQHPQVDLWIFSTDEVPDMATQTIDLAVRDDISSQAECSFKVL
HADRLYPACHPSLLSVPSAQRTTLHGEREMDWSHWAVESGIDVGQQDQGLNFSDPGLLLDAACGGLGIALVSQLLSRKAR
ADGLLQPLVDETIRGPNWALLTHRDSENDPMARSFTEWLLANLSDP

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=291, Percent_Identity=32.9896907216495, Blast_Score=150, Evalue=7e-38,
Organism=Escherichia coli, GI1786448, Length=253, Percent_Identity=35.9683794466403, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI1788706, Length=295, Percent_Identity=29.8305084745763, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI157672245, Length=127, Percent_Identity=37.7952755905512, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787128, Length=141, Percent_Identity=32.6241134751773, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786401, Length=135, Percent_Identity=28.1481481481481, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787589, Length=141, Percent_Identity=32.6241134751773, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI145693105, Length=284, Percent_Identity=24.2957746478873, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787601, Length=122, Percent_Identity=31.9672131147541, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI87081978, Length=281, Percent_Identity=26.6903914590747, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI145693193, Length=141, Percent_Identity=29.7872340425532, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31824; Mature: 31824

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKHWPPLSSLRGFEAAARLGSFHKAAEELSLTQSAISQQIRSLEAYLEQPLFFRSGRSV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEE
SLTDAGHDLLSTTQAMLQQLSVGIRRLGQYQKPNQLVLNTTPAFARHWLLPRLGDFRRQH
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHC
PQVDLWIFSTDEVPDMATQTIDLAVRDDISSQAECSFKVLHADRLYPACHPSLLSVPSAQ
CCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEHHCCCCCCCCHHHCCCCCH
RTTLHGEREMDWSHWAVESGIDVGQQDQGLNFSDPGLLLDAACGGLGIALVSQLLSRKAR
HHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEHHHHCHHHHHHHHHHHHHHHH
ADGLLQPLVDETIRGPNWALLTHRDSENDPMARSFTEWLLANLSDP
HHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLKHWPPLSSLRGFEAAARLGSFHKAAEELSLTQSAISQQIRSLEAYLEQPLFFRSGRSV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEE
SLTDAGHDLLSTTQAMLQQLSVGIRRLGQYQKPNQLVLNTTPAFARHWLLPRLGDFRRQH
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHC
PQVDLWIFSTDEVPDMATQTIDLAVRDDISSQAECSFKVLHADRLYPACHPSLLSVPSAQ
CCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEHHCCCCCCCCHHHCCCCCH
RTTLHGEREMDWSHWAVESGIDVGQQDQGLNFSDPGLLLDAACGGLGIALVSQLLSRKAR
HHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEHHHHCHHHHHHHHHHHHHHHH
ADGLLQPLVDETIRGPNWALLTHRDSENDPMARSFTEWLLANLSDP
HHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]