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The map label for this gene is dmlR [H]

Identifier: 77458585

GI number: 77458585

Start: 2716241

End: 2717206

Strand: Direct

Name: dmlR [H]

Synonym: Pfl01_2358

Alternate gene names: 77458585

Gene position: 2716241-2717206 (Clockwise)

Preceding gene: 77458578

Following gene: 77458589

Centisome position: 42.19

GC content: 61.28

Gene sequence:

>966_bases
ATGGTATCCTTCGCAAAATGCGTGATAAACAGCGGAAGCATGCGGCCTATGAACACGAATCGTGACCAATTTCCCTTGCC
CGAAGACCTCAAGGTGTTCCTTACCGTCATCCGCAAGAACAGCTTTGCCAGCGCCGCCGACGAGTTGGGCTTTTCACCGG
CCTACGTCAGCAAGCGCATCTCGGTGCTGGAAACCACGCTGGCGAGCAAGCTGCTGCACCGCACCACCCGACGCATCGCG
CTGACTGATGACGGCGAGCGCGTACGAATTTGGGCGGAGAAATTGCTGGGAGATTTCGATCAGTTTCTCGGCGAGATTTC
CCAGGCCCGGCACCAGCCGGCGGGGTCGCTGCACATTTGCAGCAGCTTCGGTTTCGGCCGTAATCATGTGGCGCCGGCGA
TCAGTCAGTTGTCCCGGGCGTGCCCGAAACTCGACATTCGGCTGGACGTGTTCGACCGGGTGGTCGATCTGGTGGGCGAG
GGCTTCGATCTGGAAATCCTCGTCGGCGATGACCTGCCGGGCCAGCATCTGGCGCGCAAACTGGTGAGCAATCGGCGCGT
GCTGTGCGCCACGCCGGAATACCTCGAACGCCGGGGTACGCCGCAATCTCTCGAAGACCTGAAAGACCACGATTGTCTGG
TGCTCAAGGAGCGCAACAACGCGTTCGGCATCTGGACCCTGAGCAAGGACGGGCAAGAAGAGACGGTGCGGGTGAATGGC
CCGCTGTCCTCCAACAGCGGCGAGATTGTGCTGGAGTGGGCGCTGAGCGGCGGCGGGATCCTGTTGCGCTCGATGTGGGA
CGTCAAACCGATGCTCGAGCAGGGCAGGCTGGTGCAGGTGCTGGACGGCTACACCCAGAGCGCCAACGTCTGGGCGGTCT
ATCCGACCCGGCTCAGCGAGTCGGCAAAGCTGCGGGTATGCGTGGAGTTTCTGGAAGAGTACTTCCGCGATTTGTCCGTG
GAATGA

Upstream 100 bases:

>100_bases
TCAAGGGAGTCGCTCATGACGCATGTCCTCATGTTTGTTTTTATCGAGGATTGACGGGAAACCACGGTGCACCGGGCTCG
GGTGGCGAGGCGGTGATTCG

Downstream 100 bases:

>100_bases
AAACGGGCGGTTCAATGAACCGCCCGTTTTTTTGCCATCAACCCAACCAGGGATTGTCCGCCAGATGCCGTGCCTCAAAG
GCGCGGATCTGCTCGGTGCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MVSFAKCVINSGSMRPMNTNRDQFPLPEDLKVFLTVIRKNSFASAADELGFSPAYVSKRISVLETTLASKLLHRTTRRIA
LTDDGERVRIWAEKLLGDFDQFLGEISQARHQPAGSLHICSSFGFGRNHVAPAISQLSRACPKLDIRLDVFDRVVDLVGE
GFDLEILVGDDLPGQHLARKLVSNRRVLCATPEYLERRGTPQSLEDLKDHDCLVLKERNNAFGIWTLSKDGQEETVRVNG
PLSSNSGEIVLEWALSGGGILLRSMWDVKPMLEQGRLVQVLDGYTQSANVWAVYPTRLSESAKLRVCVEFLEEYFRDLSV
E

Sequences:

>Translated_321_residues
MVSFAKCVINSGSMRPMNTNRDQFPLPEDLKVFLTVIRKNSFASAADELGFSPAYVSKRISVLETTLASKLLHRTTRRIA
LTDDGERVRIWAEKLLGDFDQFLGEISQARHQPAGSLHICSSFGFGRNHVAPAISQLSRACPKLDIRLDVFDRVVDLVGE
GFDLEILVGDDLPGQHLARKLVSNRRVLCATPEYLERRGTPQSLEDLKDHDCLVLKERNNAFGIWTLSKDGQEETVRVNG
PLSSNSGEIVLEWALSGGGILLRSMWDVKPMLEQGRLVQVLDGYTQSANVWAVYPTRLSESAKLRVCVEFLEEYFRDLSV
E
>Mature_321_residues
MVSFAKCVINSGSMRPMNTNRDQFPLPEDLKVFLTVIRKNSFASAADELGFSPAYVSKRISVLETTLASKLLHRTTRRIA
LTDDGERVRIWAEKLLGDFDQFLGEISQARHQPAGSLHICSSFGFGRNHVAPAISQLSRACPKLDIRLDVFDRVVDLVGE
GFDLEILVGDDLPGQHLARKLVSNRRVLCATPEYLERRGTPQSLEDLKDHDCLVLKERNNAFGIWTLSKDGQEETVRVNG
PLSSNSGEIVLEWALSGGGILLRSMWDVKPMLEQGRLVQVLDGYTQSANVWAVYPTRLSESAKLRVCVEFLEEYFRDLSV
E

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=296, Percent_Identity=53.0405405405405, Blast_Score=313, Evalue=8e-87,
Organism=Escherichia coli, GI1789440, Length=293, Percent_Identity=49.4880546075085, Blast_Score=311, Evalue=5e-86,
Organism=Escherichia coli, GI1786401, Length=292, Percent_Identity=30.1369863013699, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI145693193, Length=291, Percent_Identity=30.5841924398625, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1787128, Length=292, Percent_Identity=30.1369863013699, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1787589, Length=287, Percent_Identity=25.4355400696864, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1789639, Length=291, Percent_Identity=26.8041237113402, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1788706, Length=191, Percent_Identity=30.8900523560209, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789173, Length=272, Percent_Identity=27.5735294117647, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786448, Length=249, Percent_Identity=25.3012048192771, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790208, Length=168, Percent_Identity=29.7619047619048, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35965; Mature: 35965

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSFAKCVINSGSMRPMNTNRDQFPLPEDLKVFLTVIRKNSFASAADELGFSPAYVSKRI
CCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHH
SVLETTLASKLLHRTTRRIALTDDGERVRIWAEKLLGDFDQFLGEISQARHQPAGSLHIC
HHHHHHHHHHHHHHHHHEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
SSFGFGRNHVAPAISQLSRACPKLDIRLDVFDRVVDLVGEGFDLEILVGDDLPGQHLARK
ECCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHH
LVSNRRVLCATPEYLERRGTPQSLEDLKDHDCLVLKERNNAFGIWTLSKDGQEETVRVNG
HHHCCEEEEECHHHHHHCCCCCHHHHCCCCCEEEEEECCCEEEEEEECCCCCCCEEEEEC
PLSSNSGEIVLEWALSGGGILLRSMWDVKPMLEQGRLVQVLDGYTQSANVWAVYPTRLSE
CCCCCCCCEEEEEEECCCCEEEHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEECCCCCC
SAKLRVCVEFLEEYFRDLSVE
CHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVSFAKCVINSGSMRPMNTNRDQFPLPEDLKVFLTVIRKNSFASAADELGFSPAYVSKRI
CCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHH
SVLETTLASKLLHRTTRRIALTDDGERVRIWAEKLLGDFDQFLGEISQARHQPAGSLHIC
HHHHHHHHHHHHHHHHHEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
SSFGFGRNHVAPAISQLSRACPKLDIRLDVFDRVVDLVGEGFDLEILVGDDLPGQHLARK
ECCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHH
LVSNRRVLCATPEYLERRGTPQSLEDLKDHDCLVLKERNNAFGIWTLSKDGQEETVRVNG
HHHCCEEEEECHHHHHHCCCCCHHHHCCCCCEEEEEECCCEEEEEEECCCCCCCEEEEEC
PLSSNSGEIVLEWALSGGGILLRSMWDVKPMLEQGRLVQVLDGYTQSANVWAVYPTRLSE
CCCCCCCCEEEEEEECCCCEEEHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEECCCCCC
SAKLRVCVEFLEEYFRDLSVE
CHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]