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The map label for this gene is xylF [H]

Identifier: 77458529

GI number: 77458529

Start: 2654301

End: 2655302

Strand: Reverse

Name: xylF [H]

Synonym: Pfl01_2302

Alternate gene names: 77458529

Gene position: 2655302-2654301 (Counterclockwise)

Preceding gene: 77458530

Following gene: 77458528

Centisome position: 41.24

GC content: 59.38

Gene sequence:

>1002_bases
ATGAAGAATGTAAAACGCACGCTGCTGGCCAGCGCCCTGGCGCTATTGTCGTTACCGGTTATGGCCGATGCTGCCCACCC
GAAAATCGGCTTCTCCATCGATGACCTGCGACTGGAACGCTGGTCCCGAGACCGGGATTACTTCGTTGCGGCAGCGGAGA
AAATGGACGCCAAGGTCTTCGTGCAGTCGGCCGATGCCAACGAGCAGAAGCAGATTTCGCAAATCGAAAACCTCATTTCC
CGTGGCGTCGATGTCATCGTCATCGTGCCGTTCAACGCCACCGTGCTGACCAATGCGGTCGCCGAAGCGAAGAAAGCCGG
CATCAAGGTCGTGTCCTATGACCGTCTGATCCTCAACGCCGATATCGACGCCTACATCTCCTTCGATAACGAAAAGGTCG
GCGAGATGCAGGCCAGCGGCGTGCTGCACGCAGCGCCCAAGGGCAATTACTTCCTGCTGGGTGGCGCGCCCACCGACAAC
AACGCGAAAGTGTTGCGCGAAGGTCAGATGAAGGTGCTGCAACCGGCCATCGACAAGGGCGATATCAAGATCGTCGGCCA
GCAGTGGGTGAAGGAATGGAACCCGACAGAAGCGCTGAGCATTGTGGAAAACGCCTTGACCCGCAACGACAACAAAATCG
ACGGCATCGTCGCCTCCAACGACGCCACGGCCGGTGGCGCCATTCAGGCCCTGGCCGCTCAGCAACTGGCCGGCAAGGTG
CCGATCTCCGGACAGGACGCGGACCTGGCAGCGGTCAAGCGGGTGATCTCCGGCACTCAGACCATGACGGTGTACAAGCC
GCTGAAACTGATTGCCTCCGAAGCCGCCAAACTCTCGGTGCAACTGGCGCGCAACGAGAAACCCGCCTACAGCTCGCAAT
ACGACAACGGCAGCAAAAAAGTCGACACCATCCTGCTCACCCCGACCCCGCTGACCAAGGACAACATCGACCTGCTGGAA
CAGGACGGCTTCTATACCAAGGCGCAGATCGCCGGAAAGTGA

Upstream 100 bases:

>100_bases
ATCTGTAGTCCGTTTCGCCTACTGATTCTCTACAGTTCCACCAGCCCGATATTCATCGTCAGGCCGTGTCTTTCAAACAA
AAATAAAAGGACGCACCACC

Downstream 100 bases:

>100_bases
CACTCGTTGAGTGATGTCCCCGCAGGCGTTCTGCGCCTGCGGGATCAGTCCTGTATCTGCCGCTTATGAGTCTCTGCCAT
GTCCGACTACCTGCTGCAAA

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; xylose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MKNVKRTLLASALALLSLPVMADAAHPKIGFSIDDLRLERWSRDRDYFVAAAEKMDAKVFVQSADANEQKQISQIENLIS
RGVDVIVIVPFNATVLTNAVAEAKKAGIKVVSYDRLILNADIDAYISFDNEKVGEMQASGVLHAAPKGNYFLLGGAPTDN
NAKVLREGQMKVLQPAIDKGDIKIVGQQWVKEWNPTEALSIVENALTRNDNKIDGIVASNDATAGGAIQALAAQQLAGKV
PISGQDADLAAVKRVISGTQTMTVYKPLKLIASEAAKLSVQLARNEKPAYSSQYDNGSKKVDTILLTPTPLTKDNIDLLE
QDGFYTKAQIAGK

Sequences:

>Translated_333_residues
MKNVKRTLLASALALLSLPVMADAAHPKIGFSIDDLRLERWSRDRDYFVAAAEKMDAKVFVQSADANEQKQISQIENLIS
RGVDVIVIVPFNATVLTNAVAEAKKAGIKVVSYDRLILNADIDAYISFDNEKVGEMQASGVLHAAPKGNYFLLGGAPTDN
NAKVLREGQMKVLQPAIDKGDIKIVGQQWVKEWNPTEALSIVENALTRNDNKIDGIVASNDATAGGAIQALAAQQLAGKV
PISGQDADLAAVKRVISGTQTMTVYKPLKLIASEAAKLSVQLARNEKPAYSSQYDNGSKKVDTILLTPTPLTKDNIDLLE
QDGFYTKAQIAGK
>Mature_333_residues
MKNVKRTLLASALALLSLPVMADAAHPKIGFSIDDLRLERWSRDRDYFVAAAEKMDAKVFVQSADANEQKQISQIENLIS
RGVDVIVIVPFNATVLTNAVAEAKKAGIKVVSYDRLILNADIDAYISFDNEKVGEMQASGVLHAAPKGNYFLLGGAPTDN
NAKVLREGQMKVLQPAIDKGDIKIVGQQWVKEWNPTEALSIVENALTRNDNKIDGIVASNDATAGGAIQALAAQQLAGKV
PISGQDADLAAVKRVISGTQTMTVYKPLKLIASEAAKLSVQLARNEKPAYSSQYDNGSKKVDTILLTPTPLTKDNIDLLE
QDGFYTKAQIAGK

Specific function: Involved in the high-affinity D-xylose membrane transport system. Binds with high affinity to xylose [H]

COG id: COG4213

COG function: function code G; ABC-type xylose transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1789990, Length=312, Percent_Identity=60.8974358974359, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1790526, Length=265, Percent_Identity=28.3018867924528, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761
- InterPro:   IPR013456 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35902; Mature: 35902

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNVKRTLLASALALLSLPVMADAAHPKIGFSIDDLRLERWSRDRDYFVAAAEKMDAKVF
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHCCEEEE
VQSADANEQKQISQIENLISRGVDVIVIVPFNATVLTNAVAEAKKAGIKVVSYDRLILNA
EEECCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCEEEEEECEEEEEC
DIDAYISFDNEKVGEMQASGVLHAAPKGNYFLLGGAPTDNNAKVLREGQMKVLQPAIDKG
CCEEEEEECCCHHCCHHHCCEEEECCCCCEEEEECCCCCCCHHHHHCCCHHHHHHCCCCC
DIKIVGQQWVKEWNPTEALSIVENALTRNDNKIDGIVASNDATAGGAIQALAAQQLAGKV
CEEEEEHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCC
PISGQDADLAAVKRVISGTQTMTVYKPLKLIASEAAKLSVQLARNEKPAYSSQYDNGSKK
CCCCCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCE
VDTILLTPTPLTKDNIDLLEQDGFYTKAQIAGK
EEEEEECCCCCCCCCCHHHHCCCCEEEEEECCC
>Mature Secondary Structure
MKNVKRTLLASALALLSLPVMADAAHPKIGFSIDDLRLERWSRDRDYFVAAAEKMDAKVF
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHCCEEEE
VQSADANEQKQISQIENLISRGVDVIVIVPFNATVLTNAVAEAKKAGIKVVSYDRLILNA
EEECCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCEEEEEECEEEEEC
DIDAYISFDNEKVGEMQASGVLHAAPKGNYFLLGGAPTDNNAKVLREGQMKVLQPAIDKG
CCEEEEEECCCHHCCHHHCCEEEECCCCCEEEEECCCCCCCHHHHHCCCHHHHHHCCCCC
DIKIVGQQWVKEWNPTEALSIVENALTRNDNKIDGIVASNDATAGGAIQALAAQQLAGKV
CEEEEEHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCC
PISGQDADLAAVKRVISGTQTMTVYKPLKLIASEAAKLSVQLARNEKPAYSSQYDNGSKK
CCCCCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCE
VDTILLTPTPLTKDNIDLLEQDGFYTKAQIAGK
EEEEEECCCCCCCCCCHHHHCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; xylose [Periplasm]; H2O [C]

Specific reaction: ATP + xylose [Periplasm] + H2O = ADP + phosphate + xylose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 8581399; 9298646 [H]