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The map label for this gene is nemA [H]

Identifier: 77458470

GI number: 77458470

Start: 2573238

End: 2574302

Strand: Direct

Name: nemA [H]

Synonym: Pfl01_2243

Alternate gene names: 77458470

Gene position: 2573238-2574302 (Clockwise)

Preceding gene: 77458469

Following gene: 77458471

Centisome position: 39.97

GC content: 62.35

Gene sequence:

>1065_bases
ATGCAAATGGGCGCCCTGCAATTGACCAACCGGATTGTCATGGCGCCCGTGACCCGCAGCCGGATGGCTGAAGACGGCGT
TCCGAACGAGTTGCACGCTACTTACTACGCACAGCGCGCGAGTGCCGGCTTGATCATTGCCGAAGCGACAAACATTTCGG
CGCAGGGGCGTGGCTACGCCATGACGCCGGGGATCTGGTCGGACGCGCAGGGGGCGGGGTGGAAAAAAGTCACGGACGCC
GTCCATGCCGCAGGTGGCAGGATTGTCAGCCAGCTGTGGCACGTGGGGCGTTTTTCCAGCGTCGAGTTGCAGCCCGACCG
CGCCGCTCCCGTTGCGCCGTCGGCGATCAAGGCAGAAGGCAGCACCTACACCGAGAACGGCTTTGTCGAAGTGTCGATGC
CCCGAGCGCTGGAGACATCGGAGATTCCCGGCATCATCGAGCAATACAAGCGCGCCGCTGAAAATGCCAGGCGTGCCGGT
TTCGATGGCGTGGAGGTTCATTCGGCGAACAGCTATCTGCTCGACCAGTTTCTGCGTGACTCGACCAATCAGCGCACTGA
CCGGTATGGCGGCACCATTGAGAACCGCGCCCGACTGACGCTTGAAGTCACTGAAGCGGTGATTTCGATCTGGGGCAGTG
ACCGCGTCGGCATTCGTCTTTCGCCGGTCACGCCCGATGCCGGAAACACACCTCCCGACAGCAATGTCATGGCGACCTAC
GGATATCTGATTCAGCAGCTCAACCGGCTCAATCTGGCCTATCTGCACTTCGTCGAAGGCGCCACCGCGACCTCGCGCAC
GGTTCCTGATGGCGTTGATCTGGATGCATTGAGCGCGCAGTTCGAAGGCCCCTTCATCGGCAATAACAACTACGACCTCG
AGATGGCACTGGAGCGCCGGGCGCAGGGCCGGATTGACGCGGTGGCGTTCGGGCGCCTGTTCATTTCCAACCCCGACCTC
GTGGAGCGCCTGCGTCGGGGCACCGAACTGACCATCGCACCACGCGAGAGCTACTACGGAGGCGGCGCCAAGGGCTACAC
CGACTGGCCAACGGCTGACCATTGA

Upstream 100 bases:

>100_bases
GTACAGGCGATTTTTTTCATCAAGTTACTAGACGACCGGTCTGATATTGAAGGTATGCAACATGATTGATAACAGTCGCA
ACACGGATCTTTTTTCTCCC

Downstream 100 bases:

>100_bases
TTTGTGCGCCCCGTGCCGGATCGGCGCAGGGCGGCTCGATTGAAAAACACATTCACAGCAATCAGGAGTTCATCATGAGC
CATCCAACTTACGTGCAGGA

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MQMGALQLTNRIVMAPVTRSRMAEDGVPNELHATYYAQRASAGLIIAEATNISAQGRGYAMTPGIWSDAQGAGWKKVTDA
VHAAGGRIVSQLWHVGRFSSVELQPDRAAPVAPSAIKAEGSTYTENGFVEVSMPRALETSEIPGIIEQYKRAAENARRAG
FDGVEVHSANSYLLDQFLRDSTNQRTDRYGGTIENRARLTLEVTEAVISIWGSDRVGIRLSPVTPDAGNTPPDSNVMATY
GYLIQQLNRLNLAYLHFVEGATATSRTVPDGVDLDALSAQFEGPFIGNNNYDLEMALERRAQGRIDAVAFGRLFISNPDL
VERLRRGTELTIAPRESYYGGGAKGYTDWPTADH

Sequences:

>Translated_354_residues
MQMGALQLTNRIVMAPVTRSRMAEDGVPNELHATYYAQRASAGLIIAEATNISAQGRGYAMTPGIWSDAQGAGWKKVTDA
VHAAGGRIVSQLWHVGRFSSVELQPDRAAPVAPSAIKAEGSTYTENGFVEVSMPRALETSEIPGIIEQYKRAAENARRAG
FDGVEVHSANSYLLDQFLRDSTNQRTDRYGGTIENRARLTLEVTEAVISIWGSDRVGIRLSPVTPDAGNTPPDSNVMATY
GYLIQQLNRLNLAYLHFVEGATATSRTVPDGVDLDALSAQFEGPFIGNNNYDLEMALERRAQGRIDAVAFGRLFISNPDL
VERLRRGTELTIAPRESYYGGGAKGYTDWPTADH
>Mature_354_residues
MQMGALQLTNRIVMAPVTRSRMAEDGVPNELHATYYAQRASAGLIIAEATNISAQGRGYAMTPGIWSDAQGAGWKKVTDA
VHAAGGRIVSQLWHVGRFSSVELQPDRAAPVAPSAIKAEGSTYTENGFVEVSMPRALETSEIPGIIEQYKRAAENARRAG
FDGVEVHSANSYLLDQFLRDSTNQRTDRYGGTIENRARLTLEVTEAVISIWGSDRVGIRLSPVTPDAGNTPPDSNVMATY
GYLIQQLNRLNLAYLHFVEGATATSRTVPDGVDLDALSAQFEGPFIGNNNYDLEMALERRAQGRIDAVAFGRLFISNPDL
VERLRRGTELTIAPRESYYGGGAKGYTDWPTADH

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=357, Percent_Identity=53.781512605042, Blast_Score=342, Evalue=2e-95,
Organism=Escherichia coli, GI1789463, Length=225, Percent_Identity=37.7777777777778, Blast_Score=111, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17559802, Length=203, Percent_Identity=34.4827586206897, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17559804, Length=201, Percent_Identity=31.3432835820896, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17564188, Length=166, Percent_Identity=29.5180722891566, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17540738, Length=205, Percent_Identity=30.7317073170732, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17565138, Length=140, Percent_Identity=34.2857142857143, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI72001454, Length=301, Percent_Identity=25.5813953488372, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6325086, Length=371, Percent_Identity=39.3530997304582, Blast_Score=216, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6321973, Length=373, Percent_Identity=36.9973190348526, Blast_Score=201, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 38553; Mature: 38553

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMGALQLTNRIVMAPVTRSRMAEDGVPNELHATYYAQRASAGLIIAEATNISAQGRGYA
CCCCHHHHCCEEEECCCHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCEE
MTPGIWSDAQGAGWKKVTDAVHAAGGRIVSQLWHVGRFSSVELQPDRAAPVAPSAIKAEG
ECCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCEECCC
STYTENGFVEVSMPRALETSEIPGIIEQYKRAAENARRAGFDGVEVHSANSYLLDQFLRD
CEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHC
STNQRTDRYGGTIENRARLTLEVTEAVISIWGSDRVGIRLSPVTPDAGNTPPDSNVMATY
CCCCCHHHCCCCCCCCEEEEEEHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCHHHHH
GYLIQQLNRLNLAYLHFVEGATATSRTVPDGVDLDALSAQFEGPFIGNNNYDLEMALERR
HHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCEECCCCCEEHHHHHHH
AQGRIDAVAFGRLFISNPDLVERLRRGTELTIAPRESYYGGGAKGYTDWPTADH
HCCCEEEEEECEEEECCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQMGALQLTNRIVMAPVTRSRMAEDGVPNELHATYYAQRASAGLIIAEATNISAQGRGYA
CCCCHHHHCCEEEECCCHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCEE
MTPGIWSDAQGAGWKKVTDAVHAAGGRIVSQLWHVGRFSSVELQPDRAAPVAPSAIKAEG
ECCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCEECCC
STYTENGFVEVSMPRALETSEIPGIIEQYKRAAENARRAGFDGVEVHSANSYLLDQFLRD
CEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHC
STNQRTDRYGGTIENRARLTLEVTEAVISIWGSDRVGIRLSPVTPDAGNTPPDSNVMATY
CCCCCHHHCCCCCCCCEEEEEEHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCHHHHH
GYLIQQLNRLNLAYLHFVEGATATSRTVPDGVDLDALSAQFEGPFIGNNNYDLEMALERR
HHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCEECCCCCEEHHHHHHH
AQGRIDAVAFGRLFISNPDLVERLRRGTELTIAPRESYYGGGAKGYTDWPTADH
HCCCEEEEEECEEEECCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]