Click here to switch to the map view.

The map label for this gene is nemA [H]

Identifier: 77458456

GI number: 77458456

Start: 2557951

End: 2559075

Strand: Reverse

Name: nemA [H]

Synonym: Pfl01_2229

Alternate gene names: 77458456

Gene position: 2559075-2557951 (Counterclockwise)

Preceding gene: 77458457

Following gene: 77458451

Centisome position: 39.75

GC content: 60.98

Gene sequence:

>1125_bases
ATGAACCACAGCAGTTTCTTTACACCCGTCACCCTGGGCCGCCACATCCTGAAAAACCGCATTGTCCTGCCGCCCCTCAC
CCGCCAGCGCAGCACCCAACCGGGCAATGTCGCCAATGAATTGATGGCCGAGTATTACCAGCAACGTGCCGGTGCCGGTT
TGCTGGTGACTGAAGGCACCCAGATCGAGCCTCGTGGGCAGGGATACGCCTGGACTCCAGGCATTCATACACCCGAACAA
ATCGTCGGGTGGCGCAAGGTCACCGAGGCTGTCCACGCCAGTGACGGCGTGATTTTCGCGCAGCTATGGCATGTCGGTCG
CGTATCCCATACTGCCCTGCAACCCAATGGCGACGCACCGGTGTCGGCCTCCGCCATCGCCACCGACCGGGTCAGTGTGT
TCATCGAAACCGCGCCCGGTGTGGGCGCTCTGGTTCCGCCGTCCGCGCCTCGAGCGCTGACCACCGATGAAGTCCAGGAA
CTGGTTCAGCTATACATCCAGGCGGCGCGCAATGCCATGGACGCCGGTTTTGACGGTATCGAACTGCACTGCGCCAATGG
TTATCTGGTAAACCAGTTCATCTCCGCCCACAGCAACCTGCGCACCGATCAGTACGGCGGTTCGTTGCACAATCGCCTGC
GTTTTCTGCGCGAAGTAGTCGCCGGGGTCGCCAAGTGCATCGGCAAGGAAAAAGTCGGTGTGCGCTTCGCGCCGCTGTTC
ACCACCACCGATGAAGCGCGGACTTACCTGGGCATGGTCGAGGAAGATCCGCACACCACTTACATCGAAGCGATCAAGGT
GCTGCAAGACGTCGGCATTGCTTACCTGTCTATTGCAGAAGCCGACTGGGACGACGCGCCAGTGATGCCGCAAACCTTCC
GTCGAGCGGTGCGCGACACCTTCGGCGGCGCGATCATTTACGCGGGTCGCTACAACGCCGAAAGCGGTGCACGCTTGCTG
GAATCAGGACTGGCGGATCTCGTGGCTTTCGGTCGTCCGTTCATGGCCAATCCGGACCTGCCCGCGCGCATCGCCAATGA
CTGGCCGCTGAATTCGCTGAACCCGGCTACGGTGTATGGCGGTGATGCCGAAGGCTATGTGGATTACCCGGTGTACGGAG
AGTAG

Upstream 100 bases:

>100_bases
CGAACAACTGATCCGGACACCGTGTGTGCGTTGCACGGTGTTCTTTTTTCGCCACCTGAATAGTCGACTGGTCTAATAAG
ACTATGAACAAGGTATTTCC

Downstream 100 bases:

>100_bases
CTTTCACTGGCGACACTTTTGGCGAGCTGATTGCGGCCCGCCCCTTTGGCTTGATACAGCGCCTGATCTGCCGCAGCGAT
CAGGCCTTGTACCCTGTCCA

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MNHSSFFTPVTLGRHILKNRIVLPPLTRQRSTQPGNVANELMAEYYQQRAGAGLLVTEGTQIEPRGQGYAWTPGIHTPEQ
IVGWRKVTEAVHASDGVIFAQLWHVGRVSHTALQPNGDAPVSASAIATDRVSVFIETAPGVGALVPPSAPRALTTDEVQE
LVQLYIQAARNAMDAGFDGIELHCANGYLVNQFISAHSNLRTDQYGGSLHNRLRFLREVVAGVAKCIGKEKVGVRFAPLF
TTTDEARTYLGMVEEDPHTTYIEAIKVLQDVGIAYLSIAEADWDDAPVMPQTFRRAVRDTFGGAIIYAGRYNAESGARLL
ESGLADLVAFGRPFMANPDLPARIANDWPLNSLNPATVYGGDAEGYVDYPVYGE

Sequences:

>Translated_374_residues
MNHSSFFTPVTLGRHILKNRIVLPPLTRQRSTQPGNVANELMAEYYQQRAGAGLLVTEGTQIEPRGQGYAWTPGIHTPEQ
IVGWRKVTEAVHASDGVIFAQLWHVGRVSHTALQPNGDAPVSASAIATDRVSVFIETAPGVGALVPPSAPRALTTDEVQE
LVQLYIQAARNAMDAGFDGIELHCANGYLVNQFISAHSNLRTDQYGGSLHNRLRFLREVVAGVAKCIGKEKVGVRFAPLF
TTTDEARTYLGMVEEDPHTTYIEAIKVLQDVGIAYLSIAEADWDDAPVMPQTFRRAVRDTFGGAIIYAGRYNAESGARLL
ESGLADLVAFGRPFMANPDLPARIANDWPLNSLNPATVYGGDAEGYVDYPVYGE
>Mature_374_residues
MNHSSFFTPVTLGRHILKNRIVLPPLTRQRSTQPGNVANELMAEYYQQRAGAGLLVTEGTQIEPRGQGYAWTPGIHTPEQ
IVGWRKVTEAVHASDGVIFAQLWHVGRVSHTALQPNGDAPVSASAIATDRVSVFIETAPGVGALVPPSAPRALTTDEVQE
LVQLYIQAARNAMDAGFDGIELHCANGYLVNQFISAHSNLRTDQYGGSLHNRLRFLREVVAGVAKCIGKEKVGVRFAPLF
TTTDEARTYLGMVEEDPHTTYIEAIKVLQDVGIAYLSIAEADWDDAPVMPQTFRRAVRDTFGGAIIYAGRYNAESGARLL
ESGLADLVAFGRPFMANPDLPARIANDWPLNSLNPATVYGGDAEGYVDYPVYGE

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=374, Percent_Identity=45.7219251336898, Blast_Score=313, Evalue=9e-87,
Organism=Escherichia coli, GI1789463, Length=358, Percent_Identity=29.3296089385475, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17565138, Length=335, Percent_Identity=27.4626865671642, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17559802, Length=278, Percent_Identity=29.8561151079137, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17559804, Length=381, Percent_Identity=24.4094488188976, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17564188, Length=381, Percent_Identity=25.4593175853018, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI72001454, Length=259, Percent_Identity=28.957528957529, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17540738, Length=257, Percent_Identity=28.7937743190661, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17566914, Length=356, Percent_Identity=22.752808988764, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6321973, Length=394, Percent_Identity=35.2791878172589, Blast_Score=211, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6325086, Length=394, Percent_Identity=34.5177664974619, Blast_Score=210, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40690; Mature: 40690

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHSSFFTPVTLGRHILKNRIVLPPLTRQRSTQPGNVANELMAEYYQQRAGAGLLVTEGT
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCC
QIEPRGQGYAWTPGIHTPEQIVGWRKVTEAVHASDGVIFAQLWHVGRVSHTALQPNGDAP
EECCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHCCHHHCEECCCCCCC
VSASAIATDRVSVFIETAPGVGALVPPSAPRALTTDEVQELVQLYIQAARNAMDAGFDGI
CCHHHEEECEEEEEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCE
ELHCANGYLVNQFISAHSNLRTDQYGGSLHNRLRFLREVVAGVAKCIGKEKVGVRFAPLF
EEEECCCHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
TTTDEARTYLGMVEEDPHTTYIEAIKVLQDVGIAYLSIAEADWDDAPVMPQTFRRAVRDT
ECCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHEEEEECCCCCCCCCCHHHHHHHHHHH
FGGAIIYAGRYNAESGARLLESGLADLVAFGRPFMANPDLPARIANDWPLNSLNPATVYG
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEC
GDAEGYVDYPVYGE
CCCCCEECCCCCCC
>Mature Secondary Structure
MNHSSFFTPVTLGRHILKNRIVLPPLTRQRSTQPGNVANELMAEYYQQRAGAGLLVTEGT
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCC
QIEPRGQGYAWTPGIHTPEQIVGWRKVTEAVHASDGVIFAQLWHVGRVSHTALQPNGDAP
EECCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHCCHHHCEECCCCCCC
VSASAIATDRVSVFIETAPGVGALVPPSAPRALTTDEVQELVQLYIQAARNAMDAGFDGI
CCHHHEEECEEEEEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCE
ELHCANGYLVNQFISAHSNLRTDQYGGSLHNRLRFLREVVAGVAKCIGKEKVGVRFAPLF
EEEECCCHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
TTTDEARTYLGMVEEDPHTTYIEAIKVLQDVGIAYLSIAEADWDDAPVMPQTFRRAVRDT
ECCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHEEEEECCCCCCCCCCHHHHHHHHHHH
FGGAIIYAGRYNAESGARLLESGLADLVAFGRPFMANPDLPARIANDWPLNSLNPATVYG
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEC
GDAEGYVDYPVYGE
CCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]