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The map label for this gene is rnhA

Identifier: 77458413

GI number: 77458413

Start: 2470094

End: 2470555

Strand: Direct

Name: rnhA

Synonym: Pfl01_2186

Alternate gene names: 77458413

Gene position: 2470094-2470555 (Clockwise)

Preceding gene: 77458412

Following gene: 77458414

Centisome position: 38.36

GC content: 60.61

Gene sequence:

>462_bases
ATGAGCGAAAGCGTCGATAGCGTAGAACTGTTCACTGACGGCGCCTGTAAAGGCAACCCCGGCCCCGGCGGCTGGGGCGC
CTTGCTGGTGTGCAAGGGTGTTGAAAAGGAACTGTGGGGCGGCGAAGCCAACACCACCAACAACCGCATGGAACTGCTCG
GCGCGATTCGCGGCCTGGAAGCCCTCAAGCGTCCGTGCGAAGTGCTGCTGGTGACTGACTCGCAATACGTGATGAAGGGC
ATCAACGAATGGATGGCCAACTGGAAGAAACGCGGCTGGAAAACCGCTGCCAAGGAGCCGGTGAAAAACGCCGATCTGTG
GAAAGCGCTCGACGAGCAGGTCAACCGCCACAAAGTCACCTGGAAATGGGTCCGCGGCCACATCGGCCACCACGGCAACG
AACGCGCCGACCAGTTGGCCAACCGTGGGGTGGATGAAGTTCGCGGTTACAAGCAGAGCTGA

Upstream 100 bases:

>100_bases
AACCGTAAACCTTCTTTTATTCATGGCCGGGTATATCCCGGCCTTGGCCATCGTCGATCCGTGATCGGCGAGGCCACGCA
TTTTTTCTGGATAGACTGGC

Downstream 100 bases:

>100_bases
ATCCTTCTCGCTGAAACACTCCCGGCGAGCGTGTTAACATCGCCGCTTTTGCACGATTGACCCCGTTGAGAGCTGAACAC
TGATGGCCACCAGATCCGTT

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 153; Mature: 152

Protein sequence:

>153_residues
MSESVDSVELFTDGACKGNPGPGGWGALLVCKGVEKELWGGEANTTNNRMELLGAIRGLEALKRPCEVLLVTDSQYVMKG
INEWMANWKKRGWKTAAKEPVKNADLWKALDEQVNRHKVTWKWVRGHIGHHGNERADQLANRGVDEVRGYKQS

Sequences:

>Translated_153_residues
MSESVDSVELFTDGACKGNPGPGGWGALLVCKGVEKELWGGEANTTNNRMELLGAIRGLEALKRPCEVLLVTDSQYVMKG
INEWMANWKKRGWKTAAKEPVKNADLWKALDEQVNRHKVTWKWVRGHIGHHGNERADQLANRGVDEVRGYKQS
>Mature_152_residues
SESVDSVELFTDGACKGNPGPGGWGALLVCKGVEKELWGGEANTTNNRMELLGAIRGLEALKRPCEVLLVTDSQYVMKGI
NEWMANWKKRGWKTAAKEPVKNADLWKALDEQVNRHKVTWKWVRGHIGHHGNERADQLANRGVDEVRGYKQS

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=146, Percent_Identity=34.2465753424658, Blast_Score=74, Evalue=5e-14,
Organism=Escherichia coli, GI1786408, Length=136, Percent_Identity=61.0294117647059, Blast_Score=180, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI115532542, Length=146, Percent_Identity=34.9315068493151, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17137476, Length=143, Percent_Identity=39.1608391608392, Blast_Score=83, Evalue=6e-17,
Organism=Drosophila melanogaster, GI45552509, Length=143, Percent_Identity=39.1608391608392, Blast_Score=83, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_PSEPF (Q3KE77)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_347918.1
- HSSP:   P0A7Y4
- ProteinModelPortal:   Q3KE77
- SMR:   Q3KE77
- STRING:   Q3KE77
- GeneID:   3715727
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_2186
- eggNOG:   COG0328
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- ProtClustDB:   PRK00203
- BioCyc:   PFLU205922:PFL_2186-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 17112; Mature: 16980

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESVDSVELFTDGACKGNPGPGGWGALLVCKGVEKELWGGEANTTNNRMELLGAIRGLE
CCCCCCHHHHHCCCCCCCCCCCCCCCHHEEECCCHHHHCCCCCCCCHHHHHHHHHHHHHH
ALKRPCEVLLVTDSQYVMKGINEWMANWKKRGWKTAAKEPVKNADLWKALDEQVNRHKVT
HHCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHH
WKWVRGHIGHHGNERADQLANRGVDEVRGYKQS
HHHHHHHHCCCCCHHHHHHHHCCHHHHCCCCCC
>Mature Secondary Structure 
SESVDSVELFTDGACKGNPGPGGWGALLVCKGVEKELWGGEANTTNNRMELLGAIRGLE
CCCCCHHHHHCCCCCCCCCCCCCCCHHEEECCCHHHHCCCCCCCCHHHHHHHHHHHHHH
ALKRPCEVLLVTDSQYVMKGINEWMANWKKRGWKTAAKEPVKNADLWKALDEQVNRHKVT
HHCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHH
WKWVRGHIGHHGNERADQLANRGVDEVRGYKQS
HHHHHHHHCCCCCHHHHHHHHCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA