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The map label for this gene is puuR [H]

Identifier: 77458352

GI number: 77458352

Start: 2412384

End: 2412932

Strand: Direct

Name: puuR [H]

Synonym: Pfl01_2125

Alternate gene names: 77458352

Gene position: 2412384-2412932 (Clockwise)

Preceding gene: 77458351

Following gene: 77458355

Centisome position: 37.47

GC content: 61.2

Gene sequence:

>549_bases
ATGGATACGGGTTCTCGACTCAAACTAGTACGCGAAAGCTACAAACTCTCCCAGCGCGAGCTGGCCCGGCGTAGCGGCGT
GACCAATGCCACCATCTCCCTGATCGAACAGAATCGTGTCAGTCCTTCCGTCAGCTCACTGAAAAAGCTGCTCGAAGGCA
TCCCGATGTCCCTGGCCGACTTCTTCACCTTCGACCAGCCGCCCCGCGAACACCAATACGTCTTCCGCGCCAACGAACAA
CCCGACCTCGGCCGCCACGGCCTGCGCCTGCTGCTGATCGGCGCCTCGGTCCCCAGCCGCCAGATGCGCTTGCTGCGCGA
GCAATACGCACCGGGCGCGAGTTCCGGGGAAGAGCCGATTGTTCACGCGGAAGGTGAGGAGTGTGGGTTGGTGACGCGCG
GCACCGTGGAGTTGACGGTGGACGGGCAGGTGAGTGTGTTGAGTGCGGGGGATGGGTATTACTTCCCGACGACGCTTCCG
CACAAGTTCCGGAATATCGGGGCGGATGAGGCGGAGATTATTAGTGCGAATACGCCGGCGAATTTTTAG

Upstream 100 bases:

>100_bases
TTCACGGTTCCGGGACACGGGAGCAACGTGATCCGCGCGCCTTGCGCGGCACCCGCCACCCACCCCCATCGCCCTTAAGT
CGTTCTCACATAAGGCTGTC

Downstream 100 bases:

>100_bases
CGTGCAGCCGTAGTTGATTCAGTATTTTTAATGGAAGGCGTTTCTCGAGTGAAACGCCTTCCAGTTGTGAGCGCTATTTT
TCGACTGTGGTGGAGGGAGT

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MDTGSRLKLVRESYKLSQRELARRSGVTNATISLIEQNRVSPSVSSLKKLLEGIPMSLADFFTFDQPPREHQYVFRANEQ
PDLGRHGLRLLLIGASVPSRQMRLLREQYAPGASSGEEPIVHAEGEECGLVTRGTVELTVDGQVSVLSAGDGYYFPTTLP
HKFRNIGADEAEIISANTPANF

Sequences:

>Translated_182_residues
MDTGSRLKLVRESYKLSQRELARRSGVTNATISLIEQNRVSPSVSSLKKLLEGIPMSLADFFTFDQPPREHQYVFRANEQ
PDLGRHGLRLLLIGASVPSRQMRLLREQYAPGASSGEEPIVHAEGEECGLVTRGTVELTVDGQVSVLSAGDGYYFPTTLP
HKFRNIGADEAEIISANTPANF
>Mature_182_residues
MDTGSRLKLVRESYKLSQRELARRSGVTNATISLIEQNRVSPSVSSLKKLLEGIPMSLADFFTFDQPPREHQYVFRANEQ
PDLGRHGLRLLLIGASVPSRQMRLLREQYAPGASSGEEPIVHAEGEECGLVTRGTVELTVDGQVSVLSAGDGYYFPTTLP
HKFRNIGADEAEIISANTPANF

Specific function: Represses puuD [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787557, Length=179, Percent_Identity=39.6648044692737, Blast_Score=133, Evalue=5e-33,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR014710 [H]

Pfam domain/function: PF07883 Cupin_2; PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 20043; Mature: 20043

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTGSRLKLVRESYKLSQRELARRSGVTNATISLIEQNRVSPSVSSLKKLLEGIPMSLAD
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHH
FFTFDQPPREHQYVFRANEQPDLGRHGLRLLLIGASVPSRQMRLLREQYAPGASSGEEPI
HHHCCCCCCCCCEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCE
VHAEGEECGLVTRGTVELTVDGQVSVLSAGDGYYFPTTLPHKFRNIGADEAEIISANTPA
EEECCCCCCEEECCEEEEEECCEEEEEECCCCEECCCCCCHHHHHCCCCCCEEEECCCCC
NF
CC
>Mature Secondary Structure
MDTGSRLKLVRESYKLSQRELARRSGVTNATISLIEQNRVSPSVSSLKKLLEGIPMSLAD
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHH
FFTFDQPPREHQYVFRANEQPDLGRHGLRLLLIGASVPSRQMRLLREQYAPGASSGEEPI
HHHCCCCCCCCCEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCE
VHAEGEECGLVTRGTVELTVDGQVSVLSAGDGYYFPTTLPHKFRNIGADEAEIISANTPA
EEECCCCCCEEECCEEEEEECCEEEEEECCCCEECCCCCCHHHHHCCCCCCEEEECCCCC
NF
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553 [H]