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The map label for this gene is puuB [H]

Identifier: 77458351

GI number: 77458351

Start: 2410894

End: 2412177

Strand: Direct

Name: puuB [H]

Synonym: Pfl01_2124

Alternate gene names: 77458351

Gene position: 2410894-2412177 (Clockwise)

Preceding gene: 77458350

Following gene: 77458352

Centisome position: 37.45

GC content: 62.15

Gene sequence:

>1284_bases
ATGAAACAACAACACGTCAACAGTTATTACGCCGCCACCCGCAACGAAGTCATCGACTTCCCGACGCTTGAAGAGTCGGT
GGAGTGCGATGTCTGCATCATCGGCGCCGGCTACACCGGCCTGTCCTCGGCACTGTTCCTGAGCGAAGCCGGCTACAAGG
TGACGGTGCTGGAAGCGGCGAAAGTCGGCTACGGCGCCAGCGGTCGCAACGGCGGCCAACTGGTCAACTCCTACAGCCGC
GACGTCGACGTGATCGAAGAGCGCTACGGCGACAAGACCGCCGAAATCCTCGGCAGCATGATCTTCGAAGGCGCCGACAT
CATCCGTTCGCGCATCAAGGAATACGACATCAAATGCGACTACCGCCCGGGCGGCATTTTCGCAGCGATGAACAACAAAC
AGCTCAAGGGCCTGGCCGAGCAAAAACGTAGCTGGGAACGTTACGGCAATAAGAACCTGAAGATGCTCGACGCTGCGGAC
ATCCGCCGCGAAGTCGGCTCCGACGCCTACGTCGGTGGCCTGCTGGACATGCAGGGCGGCCACATTCACCCGCTGAACCT
GGCCCTCGGTGAAGCCGCGGTCATCGTGCGTCTGGGCGGCAAGATCTACGAGCAGTCCGCTGCGGTGGAAATCAAGTATG
GCGAGCCCAACGTCGTGCGCACCGCCCAAGGCCAGGTCCGCGCCAAGTACCTGCTGATCGCCGGAAACGCCTACCTGCCG
CAAGGCCTCGACAACCGCGTGACCGCCAAGAGCATGCCGTGCGGCTCGCAGATCTGCGTCACCGAACCGTTGACCGAGAA
ACAGGCCCGCAGCCTGATCACCAACAACTATTGCGTCGAGGACTGCAACTACCTGCTCGACTACTACCGCCTCACCGCCG
ACAACCGTCTGCTCTACGGCGGCGGCGTGGTCTACGGCGCCCGCGAACCGGACGACATCGAAACCCTGATCCGCCCGAAG
ATCCTCAAGACCTTCCCGCAGCTCAAAGACGTGAAAATCGATTACCGCTGGACCGGCAACTTCCTGCTGACCATGTCGCG
CATGCCGCAATTCGGCCGCATCGAGAAAAACGCCTACTACATGCAGGGCTACAGCGGCCACGGCGTCACGTGCTCGCACC
TGGCCGGCAAACTGATCTCGGAAATGATCCGCGGCGACGCCGAACGCTTCGACGCCTTCGCCTCCCTGCCACACATGCCG
ATGATCGGCGGCCGTACCTTCTCGGCCCCGCTGACCGCCCTCGGCGCCGTGTACTACTCGCTGCGCGACCGTTTCGGCAT
TTGA

Upstream 100 bases:

>100_bases
CGTTCGACAAGTACACCGAGCTGAAAGCGACCTGGATCAAGCTCTAATTCTATTTACAACGGTGGACGCCGGTTTTTCCG
GCGTCCCTCGGAGAACAATA

Downstream 100 bases:

>100_bases
TTAACCTCGCCGGTGGCCCCCCCAAAAAAGGGCGCCGCCGGCTTTTTCTTGTGCCGTCCATCGAACCTGCTGAGCAACAC
CGACAAACGTGATTTAATAG

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKQQHVNSYYAATRNEVIDFPTLEESVECDVCIIGAGYTGLSSALFLSEAGYKVTVLEAAKVGYGASGRNGGQLVNSYSR
DVDVIEERYGDKTAEILGSMIFEGADIIRSRIKEYDIKCDYRPGGIFAAMNNKQLKGLAEQKRSWERYGNKNLKMLDAAD
IRREVGSDAYVGGLLDMQGGHIHPLNLALGEAAVIVRLGGKIYEQSAAVEIKYGEPNVVRTAQGQVRAKYLLIAGNAYLP
QGLDNRVTAKSMPCGSQICVTEPLTEKQARSLITNNYCVEDCNYLLDYYRLTADNRLLYGGGVVYGAREPDDIETLIRPK
ILKTFPQLKDVKIDYRWTGNFLLTMSRMPQFGRIEKNAYYMQGYSGHGVTCSHLAGKLISEMIRGDAERFDAFASLPHMP
MIGGRTFSAPLTALGAVYYSLRDRFGI

Sequences:

>Translated_427_residues
MKQQHVNSYYAATRNEVIDFPTLEESVECDVCIIGAGYTGLSSALFLSEAGYKVTVLEAAKVGYGASGRNGGQLVNSYSR
DVDVIEERYGDKTAEILGSMIFEGADIIRSRIKEYDIKCDYRPGGIFAAMNNKQLKGLAEQKRSWERYGNKNLKMLDAAD
IRREVGSDAYVGGLLDMQGGHIHPLNLALGEAAVIVRLGGKIYEQSAAVEIKYGEPNVVRTAQGQVRAKYLLIAGNAYLP
QGLDNRVTAKSMPCGSQICVTEPLTEKQARSLITNNYCVEDCNYLLDYYRLTADNRLLYGGGVVYGAREPDDIETLIRPK
ILKTFPQLKDVKIDYRWTGNFLLTMSRMPQFGRIEKNAYYMQGYSGHGVTCSHLAGKLISEMIRGDAERFDAFASLPHMP
MIGGRTFSAPLTALGAVYYSLRDRFGI
>Mature_427_residues
MKQQHVNSYYAATRNEVIDFPTLEESVECDVCIIGAGYTGLSSALFLSEAGYKVTVLEAAKVGYGASGRNGGQLVNSYSR
DVDVIEERYGDKTAEILGSMIFEGADIIRSRIKEYDIKCDYRPGGIFAAMNNKQLKGLAEQKRSWERYGNKNLKMLDAAD
IRREVGSDAYVGGLLDMQGGHIHPLNLALGEAAVIVRLGGKIYEQSAAVEIKYGEPNVVRTAQGQVRAKYLLIAGNAYLP
QGLDNRVTAKSMPCGSQICVTEPLTEKQARSLITNNYCVEDCNYLLDYYRLTADNRLLYGGGVVYGAREPDDIETLIRPK
ILKTFPQLKDVKIDYRWTGNFLLTMSRMPQFGRIEKNAYYMQGYSGHGVTCSHLAGKLISEMIRGDAERFDAFASLPHMP
MIGGRTFSAPLTALGAVYYSLRDRFGI

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=424, Percent_Identity=69.3396226415094, Blast_Score=635, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47293; Mature: 47293

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQQHVNSYYAATRNEVIDFPTLEESVECDVCIIGAGYTGLSSALFLSEAGYKVTVLEAA
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHEECCCCEEEEEEEE
KVGYGASGRNGGQLVNSYSRDVDVIEERYGDKTAEILGSMIFEGADIIRSRIKEYDIKCD
CCCCCCCCCCCHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHCCCEEE
YRPGGIFAAMNNKQLKGLAEQKRSWERYGNKNLKMLDAADIRREVGSDAYVGGLLDMQGG
CCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCEECEEEECCCC
HIHPLNLALGEAAVIVRLGGKIYEQSAAVEIKYGEPNVVRTAQGQVRAKYLLIAGNAYLP
EEEEEEEECCCEEEEEEECCHHEECCCEEEEEECCCCEEEECCCCEEEEEEEEECCCCCC
QGLDNRVTAKSMPCGSQICVTEPLTEKQARSLITNNYCVEDCNYLLDYYRLTADNRLLYG
CCCCCCCEECCCCCCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEE
GGVVYGAREPDDIETLIRPKILKTFPQLKDVKIDYRWTGNFLLTMSRMPQFGRIEKNAYY
CEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCEEE
MQGYSGHGVTCSHLAGKLISEMIRGDAERFDAFASLPHMPMIGGRTFSAPLTALGAVYYS
EECCCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
LRDRFGI
HHHHCCC
>Mature Secondary Structure
MKQQHVNSYYAATRNEVIDFPTLEESVECDVCIIGAGYTGLSSALFLSEAGYKVTVLEAA
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHEECCCCEEEEEEEE
KVGYGASGRNGGQLVNSYSRDVDVIEERYGDKTAEILGSMIFEGADIIRSRIKEYDIKCD
CCCCCCCCCCCHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHCCCEEE
YRPGGIFAAMNNKQLKGLAEQKRSWERYGNKNLKMLDAADIRREVGSDAYVGGLLDMQGG
CCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCEECEEEECCCC
HIHPLNLALGEAAVIVRLGGKIYEQSAAVEIKYGEPNVVRTAQGQVRAKYLLIAGNAYLP
EEEEEEEECCCEEEEEEECCHHEECCCEEEEEECCCCEEEECCCCEEEEEEEEECCCCCC
QGLDNRVTAKSMPCGSQICVTEPLTEKQARSLITNNYCVEDCNYLLDYYRLTADNRLLYG
CCCCCCCEECCCCCCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEE
GGVVYGAREPDDIETLIRPKILKTFPQLKDVKIDYRWTGNFLLTMSRMPQFGRIEKNAYY
CEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCEEE
MQGYSGHGVTCSHLAGKLISEMIRGDAERFDAFASLPHMPMIGGRTFSAPLTALGAVYYS
EECCCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
LRDRFGI
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]