Click here to switch to the map view.

The map label for this gene is puuD [H]

Identifier: 77458348

GI number: 77458348

Start: 2406676

End: 2407437

Strand: Reverse

Name: puuD [H]

Synonym: Pfl01_2121

Alternate gene names: 77458348

Gene position: 2407437-2406676 (Counterclockwise)

Preceding gene: 77458353

Following gene: 77458347

Centisome position: 37.39

GC content: 64.3

Gene sequence:

>762_bases
ATGGCATTCAAGCCATTGATCGGCGTTACTGCGTGCGTCAAACAGATTGGCCTGCACCCCTATCACATCAGCGGCGACAA
ATACGTTCGCGCTGTCAGCGTTGCGGCGCTGGGGTTGCCCGTCGTCATTCCTTCCCTTGGCCAACTGACTGAAATCGAAG
ATCTGCTGGGTCAGCTCGACGGTCTGTTGCTGACCGGCTCGCCGTCCAACGTGGAACCCTTCCACTATCAGGGCCCGGAC
AGCGCTCCCGGCACGGACCACGATCCGGCGCGGGATGCCACTACCCTTCCTTTATTGCGTGCAGCCATCGCGGCGGGCGT
TCCGGTACTCGGCATCTGCCGGGGTTTCCAGGAAATGAACGTGGCGTTCGGCGGCAGCCTGCACCAGAAGGTGCATGAGC
TGCCGGGCATGCTCGATCACCGCGAAGCCGACAGCCCGGATGTCGCCGTGCAATACGCCCCGGCCCATGCCGTGACGGTG
CTGCCCGGTGGCGTGTTCGAAGCGCTGGAGCTGCCGAGTGAATTCCAGGTCAACTCGATTCACAGTCAGGGCATCGACCG
CCTCGCTCCCGGTCTGCGCGCCGAAGCCGTGGCGCCGGATGGCCTGATCGAGGCGGTCTCGGTGGAGCACAGCCCGACCT
TCGCCCTCGGCGTGCAATGGCACCCGGAATGGCAAGTGCTGGACAACCCGAACTACCTGCGGATTTTCCAGGCATTCGGC
GAGGCTTGCCGCCAGCGTGCGGCGCGACGCAACCAGCGCTGA

Upstream 100 bases:

>100_bases
ACGGCGTTTAGAATTTTTTACATGGGAGTTGTTAATTAAAATCAACGAGGCTAAGCTCCGGAAAAGCTCGTTCAAGTGTC
AAACTTCGAGGTGAATAAAA

Downstream 100 bases:

>100_bases
CGCCACCCAACGATTGCAAAACCATTTACTGCCCCCGCGGGGTCGGCGGCTGCGCGCTTGCGCATCCGTCATCCGCGATC
AGCAACACACCGCGAAAACA

Product: peptidase C26

Products: NA

Alternate protein names: Gamma-Glu-GABA hydrolase [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MAFKPLIGVTACVKQIGLHPYHISGDKYVRAVSVAALGLPVVIPSLGQLTEIEDLLGQLDGLLLTGSPSNVEPFHYQGPD
SAPGTDHDPARDATTLPLLRAAIAAGVPVLGICRGFQEMNVAFGGSLHQKVHELPGMLDHREADSPDVAVQYAPAHAVTV
LPGGVFEALELPSEFQVNSIHSQGIDRLAPGLRAEAVAPDGLIEAVSVEHSPTFALGVQWHPEWQVLDNPNYLRIFQAFG
EACRQRAARRNQR

Sequences:

>Translated_253_residues
MAFKPLIGVTACVKQIGLHPYHISGDKYVRAVSVAALGLPVVIPSLGQLTEIEDLLGQLDGLLLTGSPSNVEPFHYQGPD
SAPGTDHDPARDATTLPLLRAAIAAGVPVLGICRGFQEMNVAFGGSLHQKVHELPGMLDHREADSPDVAVQYAPAHAVTV
LPGGVFEALELPSEFQVNSIHSQGIDRLAPGLRAEAVAPDGLIEAVSVEHSPTFALGVQWHPEWQVLDNPNYLRIFQAFG
EACRQRAARRNQR
>Mature_252_residues
AFKPLIGVTACVKQIGLHPYHISGDKYVRAVSVAALGLPVVIPSLGQLTEIEDLLGQLDGLLLTGSPSNVEPFHYQGPDS
APGTDHDPARDATTLPLLRAAIAAGVPVLGICRGFQEMNVAFGGSLHQKVHELPGMLDHREADSPDVAVQYAPAHAVTVL
PGGVFEALELPSEFQVNSIHSQGIDRLAPGLRAEAVAPDGLIEAVSVEHSPTFALGVQWHPEWQVLDNPNYLRIFQAFGE
ACRQRAARRNQR

Specific function: Involved in the breakdown of putrescine via hydrolysis of the gamma-glutamyl linkage of gamma-glutamyl-gamma- aminobutyrate [H]

COG id: COG2071

COG function: function code R; Predicted glutamine amidotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87081871, Length=252, Percent_Identity=44.4444444444444, Blast_Score=193, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR011697 [H]

Pfam domain/function: PF07722 Peptidase_C26 [H]

EC number: =3.5.1.94 [H]

Molecular weight: Translated: 27115; Mature: 26984

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFKPLIGVTACVKQIGLHPYHISGDKYVRAVSVAALGLPVVIPSLGQLTEIEDLLGQLD
CCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHC
GLLLTGSPSNVEPFHYQGPDSAPGTDHDPARDATTLPLLRAAIAAGVPVLGICRGFQEMN
CEEEECCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
VAFGGSLHQKVHELPGMLDHREADSPDVAVQYAPAHAVTVLPGGVFEALELPSEFQVNSI
HHHCCHHHHHHHHCCCCCCCCCCCCCCEEEEECCCEEEEECCCHHHHHHCCCCCCCHHHH
HSQGIDRLAPGLRAEAVAPDGLIEAVSVEHSPTFALGVQWHPEWQVLDNPNYLRIFQAFG
HHCCHHHHCCCCCCCCCCCCCHHHEEEECCCCCEEEEEEECCCEEEECCCCHHHHHHHHH
EACRQRAARRNQR
HHHHHHHHHCCCC
>Mature Secondary Structure 
AFKPLIGVTACVKQIGLHPYHISGDKYVRAVSVAALGLPVVIPSLGQLTEIEDLLGQLD
CCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHC
GLLLTGSPSNVEPFHYQGPDSAPGTDHDPARDATTLPLLRAAIAAGVPVLGICRGFQEMN
CEEEECCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
VAFGGSLHQKVHELPGMLDHREADSPDVAVQYAPAHAVTVLPGGVFEALELPSEFQVNSI
HHHCCHHHHHHHHCCCCCCCCCCCCCCEEEEECCCEEEEECCCHHHHHHCCCCCCCHHHH
HSQGIDRLAPGLRAEAVAPDGLIEAVSVEHSPTFALGVQWHPEWQVLDNPNYLRIFQAFG
HHCCHHHHCCCCCCCCCCCCCHHHEEEECCCCCEEEEEEECCCEEEECCCCHHHHHHHHH
EACRQRAARRNQR
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]