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The map label for this gene is potH [H]

Identifier: 77458346

GI number: 77458346

Start: 2404403

End: 2405317

Strand: Reverse

Name: potH [H]

Synonym: Pfl01_2119

Alternate gene names: 77458346

Gene position: 2405317-2404403 (Counterclockwise)

Preceding gene: 77458347

Following gene: 77458345

Centisome position: 37.36

GC content: 57.92

Gene sequence:

>915_bases
ATGAGAACCTTCAATCAGCAATTCCTGCGCCTGGTGCCCAGCGGGCGAAAGTTCGTCATCGGCATTCCGTTCATCTGGCT
GTTCCTGTTCTTCATGCTGCCGTTCTTCCTGGTGATGAAGATCAGCTTCTCGGAAGCCGCGCTGTCGATCCCGCCGTACT
CGGAGATCTACACCTACGCCGAACAGAAATTCCAGCTGCTGCTGAACATCGGCAACTACACCATGCTTGGCGAGGACGAG
CTGTACCTGTCGGCCTACCTCGGTTCGCTGAAGGTCGCGGCACTGAGCACGATGATGTGCCTGGTGATCGGCTTCCCGAT
GGCTTACGCGATCACCAAGGCCAGCAAGGAAGCGCAAAACGTCCTGTTGCTGTTGATCATGATGCCGACCTGGACCGCGA
TCCTGATCCGCGTTTACGCGTGGATGGGCATCCTCAGCAACAACGGTCTGCTTAACGCATTTTTGATGTGGACCGGGCTC
ACCGATCACCCGATCGAGATCCTCAACACCAACACCGCCGTCTACATTGGGGTCGTGTATGCCTATCTGCCGTTCATGGT
GCTGCCGCTCTACGCCAACCTGGTGAAGCACGACGGCAGCCTGTTGGAAGCCGCGCAGGATCTGGGTTCGAGCAACTTCA
ACAACTTCTGGAAAATCACCGTGCCGCTGGCCAAGAACGGCATCATCGCCGGCTGCATGCTGGTGTTCATTCCGGTGGTG
GGCGAGTTCGTGATTCCGGAACTGCTGGGCGGCCCGGAGACCCTGATGATCGGTCGCGTGCTGTGGCAAGAGTTCTTCAA
TAACCGCGACTGGCCGGTGGCGTCTGCTCTGGCGGTGGTGATGCTGTTGATCCTGATTGTGCCGATCCTGCTGTTCAACC
GCAGCCAGGCCAAAGAGATGGAGGGACGAGGATGA

Upstream 100 bases:

>100_bases
AGTCGTTCATGGCCAACGCCGAACGTCGTGGCGCGCGGCCGACCTGGGACGACAAGGTCTACGTCTGGTGGGAAGACGAC
AGCGGCGTGGTACTGCGCTC

Downstream 100 bases:

>100_bases
AACGCTTCGGATTTTCAAAGTTCATGCTGATCTTCGGCCTGATGTTCATCTATCTGCCGATGCTGATTCTGGTGATCTAC
TCGTTCAACGCCTCGAAACT

Product: binding-protein dependent transport system inner membrane protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MRTFNQQFLRLVPSGRKFVIGIPFIWLFLFFMLPFFLVMKISFSEAALSIPPYSEIYTYAEQKFQLLLNIGNYTMLGEDE
LYLSAYLGSLKVAALSTMMCLVIGFPMAYAITKASKEAQNVLLLLIMMPTWTAILIRVYAWMGILSNNGLLNAFLMWTGL
TDHPIEILNTNTAVYIGVVYAYLPFMVLPLYANLVKHDGSLLEAAQDLGSSNFNNFWKITVPLAKNGIIAGCMLVFIPVV
GEFVIPELLGGPETLMIGRVLWQEFFNNRDWPVASALAVVMLLILIVPILLFNRSQAKEMEGRG

Sequences:

>Translated_304_residues
MRTFNQQFLRLVPSGRKFVIGIPFIWLFLFFMLPFFLVMKISFSEAALSIPPYSEIYTYAEQKFQLLLNIGNYTMLGEDE
LYLSAYLGSLKVAALSTMMCLVIGFPMAYAITKASKEAQNVLLLLIMMPTWTAILIRVYAWMGILSNNGLLNAFLMWTGL
TDHPIEILNTNTAVYIGVVYAYLPFMVLPLYANLVKHDGSLLEAAQDLGSSNFNNFWKITVPLAKNGIIAGCMLVFIPVV
GEFVIPELLGGPETLMIGRVLWQEFFNNRDWPVASALAVVMLLILIVPILLFNRSQAKEMEGRG
>Mature_304_residues
MRTFNQQFLRLVPSGRKFVIGIPFIWLFLFFMLPFFLVMKISFSEAALSIPPYSEIYTYAEQKFQLLLNIGNYTMLGEDE
LYLSAYLGSLKVAALSTMMCLVIGFPMAYAITKASKEAQNVLLLLIMMPTWTAILIRVYAWMGILSNNGLLNAFLMWTGL
TDHPIEILNTNTAVYIGVVYAYLPFMVLPLYANLVKHDGSLLEAAQDLGSSNFNNFWKITVPLAKNGIIAGCMLVFIPVV
GEFVIPELLGGPETLMIGRVLWQEFFNNRDWPVASALAVVMLLILIVPILLFNRSQAKEMEGRG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=291, Percent_Identity=59.7938144329897, Blast_Score=350, Evalue=1e-97,
Organism=Escherichia coli, GI1787369, Length=272, Percent_Identity=40.0735294117647, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1787713, Length=247, Percent_Identity=27.5303643724696, Blast_Score=94, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34179; Mature: 34179

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTFNQQFLRLVPSGRKFVIGIPFIWLFLFFMLPFFLVMKISFSEAALSIPPYSEIYTYA
CCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHH
EQKFQLLLNIGNYTMLGEDELYLSAYLGSLKVAALSTMMCLVIGFPMAYAITKASKEAQN
HHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLLLLIMMPTWTAILIRVYAWMGILSNNGLLNAFLMWTGLTDHPIEILNTNTAVYIGVVY
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHEECCCCHHHHHHHH
AYLPFMVLPLYANLVKHDGSLLEAAQDLGSSNFNNFWKITVPLAKNGIIAGCMLVFIPVV
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHH
GEFVIPELLGGPETLMIGRVLWQEFFNNRDWPVASALAVVMLLILIVPILLFNRSQAKEM
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHC
EGRG
CCCC
>Mature Secondary Structure
MRTFNQQFLRLVPSGRKFVIGIPFIWLFLFFMLPFFLVMKISFSEAALSIPPYSEIYTYA
CCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHH
EQKFQLLLNIGNYTMLGEDELYLSAYLGSLKVAALSTMMCLVIGFPMAYAITKASKEAQN
HHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLLLLIMMPTWTAILIRVYAWMGILSNNGLLNAFLMWTGLTDHPIEILNTNTAVYIGVVY
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHEECCCCHHHHHHHH
AYLPFMVLPLYANLVKHDGSLLEAAQDLGSSNFNNFWKITVPLAKNGIIAGCMLVFIPVV
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHH
GEFVIPELLGGPETLMIGRVLWQEFFNNRDWPVASALAVVMLLILIVPILLFNRSQAKEM
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHC
EGRG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]