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The map label for this gene is rstB [H]

Identifier: 77458312

GI number: 77458312

Start: 2371944

End: 2373248

Strand: Reverse

Name: rstB [H]

Synonym: Pfl01_2085

Alternate gene names: 77458312

Gene position: 2373248-2371944 (Counterclockwise)

Preceding gene: 77458313

Following gene: 77458311

Centisome position: 36.86

GC content: 62.99

Gene sequence:

>1305_bases
ATGAATGTCGCCCTGCGCTGGCCGCGCACCCTCGCCTCCCGCTTGTCGCTGATTTTCCTGATCGGGCTGATCCTCGCTCA
GGCGCTGTCGTTCGGCGCGCAATATTACGAGCGCTACGAAAGCGCGAAAAACACCATGCTCGGCAACCTCGAAACCGACG
TCTCGACCTCGATTGCCATTCTTGATCGTCTGCCCGCCGAAGAACGCCCGATGTGGCTCAAACGCCTGGCGCGCAAGAAC
TACGGTTATCTGCTGAGCGAAGGCGAACCGGGCACGCCGCTGGACCCGCAGGACGTACCGATTGCCGTCACCTCGATTAC
CGACGCAATTGGCGAGGCCTATCCGCTGACGTTCACCGATATTCCCGGCCCGATAAAACACTTTCAAGGCCACCTGCGCC
TGAGCGACGGTAGCCCGGTGACCATCGATGTGCGCCCGTCGATGGTGCCGCTGTCGCCGTGGCTGCCGGTGGTGTTACTC
GGGCAACTGGCGCTGATGCTCGCCTGCACCTGGCTGGCGGTGCGTATCGCCATCCGCCCCCTCACCCGTCTCGCCAACGC
GGTGGAAACCCTCGATCCCAACGCGCACCCGATCAACCTCGACGAAAAAGGCCCGAACGAAGTGGCTTACGCCGCCCGCG
CTTTCAACTCGATGCAGGCGCGCATCGCCGCGTACCTCAAAGAACGCATGCAACTGCTGGCGGCGATTTCCCACGACCTG
CAAACCCCGATCACCCGCATGAAGCTGCGGGCGGAGTTCATGGACGATTCAGCCGAGAAAGACAAACTGTGGAACGACCT
CGGTGAGATGGAGCATCTGGTGCGCGAAGGTGTGGCCTACGCCCGCAGCATCCACGGCTCGACTGAAGAAAGCCGACGCA
CCAATCTGGACTCGTTCCTCGACAGCCTGGTGTTCGACTATCAGGACATGGGTAAGGAAGTGCAGTTGAGCGGCAAGAGT
GACGCGGTCATCGACACCCGCCCCCACGCGTTGCGCCGGGTGCTGGTCAACCTGACCGACAACGCGCTGAAGTTCGCCGG
GGCTGCCGAGCTGCTGATTCAACGCGACAAGAGCGGCCTGTCGATCAAGGTCATGGATCGCGGCCCGGGCATCGCCGAAG
AAGAGCTGGCCCAGGTGATGGAGCCTTTCTACCGCGTGGAAAACTCGCGCAACCGCAGCACCGGCGGCACCGGGCTGGGG
CTGGCGATCGCCCAGCAACTGGCGCTGGCGCTGGGCGGTTCGCTGACGCTGAGCAATCGCGAAGGTGGCGGGTTGTGTGC
GGAGCTGAAACTGCCATTGAGCTGA

Upstream 100 bases:

>100_bases
AGCGCCTGCTGGATGACGCCCGTGAACCGGCCTACATCAAGACCGTGCGCAGCGAAGGCTATGTGTTCTCGTTGCCGGTC
GAAGTCCTCGGAGCCCCCGC

Downstream 100 bases:

>100_bases
GCACCAGCGGTCGCTACTGTCAGATCTGACAGTAGCCGGCCAGGCAGAATTATTGTGGGATGACACCTCGCTCAGCCGTC
GCGTGGTGAACCATGTCTCT

Product: periplasmic sensor Signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MNVALRWPRTLASRLSLIFLIGLILAQALSFGAQYYERYESAKNTMLGNLETDVSTSIAILDRLPAEERPMWLKRLARKN
YGYLLSEGEPGTPLDPQDVPIAVTSITDAIGEAYPLTFTDIPGPIKHFQGHLRLSDGSPVTIDVRPSMVPLSPWLPVVLL
GQLALMLACTWLAVRIAIRPLTRLANAVETLDPNAHPINLDEKGPNEVAYAARAFNSMQARIAAYLKERMQLLAAISHDL
QTPITRMKLRAEFMDDSAEKDKLWNDLGEMEHLVREGVAYARSIHGSTEESRRTNLDSFLDSLVFDYQDMGKEVQLSGKS
DAVIDTRPHALRRVLVNLTDNALKFAGAAELLIQRDKSGLSIKVMDRGPGIAEEELAQVMEPFYRVENSRNRSTGGTGLG
LAIAQQLALALGGSLTLSNREGGGLCAELKLPLS

Sequences:

>Translated_434_residues
MNVALRWPRTLASRLSLIFLIGLILAQALSFGAQYYERYESAKNTMLGNLETDVSTSIAILDRLPAEERPMWLKRLARKN
YGYLLSEGEPGTPLDPQDVPIAVTSITDAIGEAYPLTFTDIPGPIKHFQGHLRLSDGSPVTIDVRPSMVPLSPWLPVVLL
GQLALMLACTWLAVRIAIRPLTRLANAVETLDPNAHPINLDEKGPNEVAYAARAFNSMQARIAAYLKERMQLLAAISHDL
QTPITRMKLRAEFMDDSAEKDKLWNDLGEMEHLVREGVAYARSIHGSTEESRRTNLDSFLDSLVFDYQDMGKEVQLSGKS
DAVIDTRPHALRRVLVNLTDNALKFAGAAELLIQRDKSGLSIKVMDRGPGIAEEELAQVMEPFYRVENSRNRSTGGTGLG
LAIAQQLALALGGSLTLSNREGGGLCAELKLPLS
>Mature_434_residues
MNVALRWPRTLASRLSLIFLIGLILAQALSFGAQYYERYESAKNTMLGNLETDVSTSIAILDRLPAEERPMWLKRLARKN
YGYLLSEGEPGTPLDPQDVPIAVTSITDAIGEAYPLTFTDIPGPIKHFQGHLRLSDGSPVTIDVRPSMVPLSPWLPVVLL
GQLALMLACTWLAVRIAIRPLTRLANAVETLDPNAHPINLDEKGPNEVAYAARAFNSMQARIAAYLKERMQLLAAISHDL
QTPITRMKLRAEFMDDSAEKDKLWNDLGEMEHLVREGVAYARSIHGSTEESRRTNLDSFLDSLVFDYQDMGKEVQLSGKS
DAVIDTRPHALRRVLVNLTDNALKFAGAAELLIQRDKSGLSIKVMDRGPGIAEEELAQVMEPFYRVENSRNRSTGGTGLG
LAIAQQLALALGGSLTLSNREGGGLCAELKLPLS

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=231, Percent_Identity=35.0649350649351, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1789808, Length=278, Percent_Identity=29.8561151079137, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1790346, Length=268, Percent_Identity=29.1044776119403, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1788393, Length=269, Percent_Identity=25.2788104089219, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1786783, Length=277, Percent_Identity=27.0758122743682, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1790861, Length=300, Percent_Identity=26.6666666666667, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI87082128, Length=301, Percent_Identity=24.9169435215947, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1789149, Length=234, Percent_Identity=27.7777777777778, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786912, Length=233, Percent_Identity=30.9012875536481, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI87081816, Length=225, Percent_Identity=30.6666666666667, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI145693157, Length=108, Percent_Identity=36.1111111111111, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1789403, Length=298, Percent_Identity=25.503355704698, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790551, Length=276, Percent_Identity=25.3623188405797, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786600, Length=285, Percent_Identity=25.6140350877193, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI48994928, Length=229, Percent_Identity=27.0742358078603, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790436, Length=220, Percent_Identity=25, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47753; Mature: 47753

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVALRWPRTLASRLSLIFLIGLILAQALSFGAQYYERYESAKNTMLGNLETDVSTSIAI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
LDRLPAEERPMWLKRLARKNYGYLLSEGEPGTPLDPQDVPIAVTSITDAIGEAYPLTFTD
HHHCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEHHHHHHHHCCCCCEEECC
IPGPIKHFQGHLRLSDGSPVTIDVRPSMVPLSPWLPVVLLGQLALMLACTWLAVRIAIRP
CCCHHHHCCCCEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LTRLANAVETLDPNAHPINLDEKGPNEVAYAARAFNSMQARIAAYLKERMQLLAAISHDL
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTPITRMKLRAEFMDDSAEKDKLWNDLGEMEHLVREGVAYARSIHGSTEESRRTNLDSFL
HCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHH
DSLVFDYQDMGKEVQLSGKSDAVIDTRPHALRRVLVNLTDNALKFAGAAELLIQRDKSGL
HHHHHHHHHCCCEEEECCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SIKVMDRGPGIAEEELAQVMEPFYRVENSRNRSTGGTGLGLAIAQQLALALGGSLTLSNR
EEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCC
EGGGLCAELKLPLS
CCCCEEEEEECCCC
>Mature Secondary Structure
MNVALRWPRTLASRLSLIFLIGLILAQALSFGAQYYERYESAKNTMLGNLETDVSTSIAI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
LDRLPAEERPMWLKRLARKNYGYLLSEGEPGTPLDPQDVPIAVTSITDAIGEAYPLTFTD
HHHCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEHHHHHHHHCCCCCEEECC
IPGPIKHFQGHLRLSDGSPVTIDVRPSMVPLSPWLPVVLLGQLALMLACTWLAVRIAIRP
CCCHHHHCCCCEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LTRLANAVETLDPNAHPINLDEKGPNEVAYAARAFNSMQARIAAYLKERMQLLAAISHDL
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTPITRMKLRAEFMDDSAEKDKLWNDLGEMEHLVREGVAYARSIHGSTEESRRTNLDSFL
HCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHH
DSLVFDYQDMGKEVQLSGKSDAVIDTRPHALRRVLVNLTDNALKFAGAAELLIQRDKSGL
HHHHHHHHHCCCEEEECCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SIKVMDRGPGIAEEELAQVMEPFYRVENSRNRSTGGTGLGLAIAQQLALALGGSLTLSNR
EEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCC
EGGGLCAELKLPLS
CCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]