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The map label for this gene is argT [H]

Identifier: 77458291

GI number: 77458291

Start: 2350882

End: 2351661

Strand: Direct

Name: argT [H]

Synonym: Pfl01_2064

Alternate gene names: 77458291

Gene position: 2350882-2351661 (Clockwise)

Preceding gene: 77458290

Following gene: 77458292

Centisome position: 36.51

GC content: 58.59

Gene sequence:

>780_bases
ATGAAAAAGCTGGTTATGTTCGGTGCCCTGGCACTGTCGATGTTGTCCCTGACCGCCGTGGCCGAAGACGCCAAGCCGAT
CCGTATCGGTATCGAAGCCGGTTACCCGCCATTCTCGATGAAAACCCCTGACGGCAAACTGGCCGGTTTCGACGTGGACA
TCGGCGATGCGCTGTGTGAGCAGATGAAAGTCAAATGCACCTGGGTCGAGCAGGAATTCGACGGCCTGATCCCGGCGCTG
AAAGTGAAGAAGATCGACGCGATCCTGTCCTCGATGACCATCACTGACGACCGCAAGAAAAACGTCGATTTCACCATCAA
GTACTACCACACCCCGGCGCGCTTCGTGATGAAGGCAGGCTCTGGCGTGAAAGACCCGCTGACCGAGCTCAAAGGCAAGA
AAGTCGGCGTGCTGCGCGCCAGTACCCACGACCGCTACGCAACCGAAGTACTGGTGCCGGCCGGCATCGAGCTGGTGCGT
TACGGCTCACAGCAGGAGGCCAACCTGGACATGGTGTCCGGTCGTATCGACGCAATGCTGGCCGACTCGGTCAACCTGAG
CGACGGCTTCCTGAAAACCGACGCCGGCAAAGGCTTCGAATTCGTCGGCCCGACCTACGAAGACGCCAAGTACTTCGGCG
GCGGCGCCGGTATCGCCGTGCGTAAAGGCGATACCGAGCTGGCGGAGAAATTCAACACCGCCATCAACGAAATCCGCGCC
AACGGCAAGTACAAGCAAGTGCAGGACAAGTACTTCGACTTTGACGTGTACGGCCATTAA

Upstream 100 bases:

>100_bases
TAGACTGCCGGCCACTTCGGTTTTTTTGCGCTGTACAGCCCAAGGCCGAACCTGACAATCGAAAGCTGCCAATAAAAGCC
TCCGCACACGGGAGTAATAA

Downstream 100 bases:

>100_bases
TACGCCGTAGAAAAAAGTGGCCCCGTTGACGCGGTGGCCACTTTTTTTTCGCCCGTGATTTATCCTGTCAGCACATTTTC
AATGCGGTCACTGGGTAGCA

Product: lysine-arginine-ornithine-binding periplasmic protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MKKLVMFGALALSMLSLTAVAEDAKPIRIGIEAGYPPFSMKTPDGKLAGFDVDIGDALCEQMKVKCTWVEQEFDGLIPAL
KVKKIDAILSSMTITDDRKKNVDFTIKYYHTPARFVMKAGSGVKDPLTELKGKKVGVLRASTHDRYATEVLVPAGIELVR
YGSQQEANLDMVSGRIDAMLADSVNLSDGFLKTDAGKGFEFVGPTYEDAKYFGGGAGIAVRKGDTELAEKFNTAINEIRA
NGKYKQVQDKYFDFDVYGH

Sequences:

>Translated_259_residues
MKKLVMFGALALSMLSLTAVAEDAKPIRIGIEAGYPPFSMKTPDGKLAGFDVDIGDALCEQMKVKCTWVEQEFDGLIPAL
KVKKIDAILSSMTITDDRKKNVDFTIKYYHTPARFVMKAGSGVKDPLTELKGKKVGVLRASTHDRYATEVLVPAGIELVR
YGSQQEANLDMVSGRIDAMLADSVNLSDGFLKTDAGKGFEFVGPTYEDAKYFGGGAGIAVRKGDTELAEKFNTAINEIRA
NGKYKQVQDKYFDFDVYGH
>Mature_259_residues
MKKLVMFGALALSMLSLTAVAEDAKPIRIGIEAGYPPFSMKTPDGKLAGFDVDIGDALCEQMKVKCTWVEQEFDGLIPAL
KVKKIDAILSSMTITDDRKKNVDFTIKYYHTPARFVMKAGSGVKDPLTELKGKKVGVLRASTHDRYATEVLVPAGIELVR
YGSQQEANLDMVSGRIDAMLADSVNLSDGFLKTDAGKGFEFVGPTYEDAKYFGGGAGIAVRKGDTELAEKFNTAINEIRA
NGKYKQVQDKYFDFDVYGH

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=263, Percent_Identity=46.7680608365019, Blast_Score=241, Evalue=4e-65,
Organism=Escherichia coli, GI1788648, Length=232, Percent_Identity=46.1206896551724, Blast_Score=223, Evalue=8e-60,
Organism=Escherichia coli, GI1787088, Length=252, Percent_Identity=39.2857142857143, Blast_Score=177, Evalue=9e-46,
Organism=Escherichia coli, GI1787085, Length=252, Percent_Identity=38.8888888888889, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1788228, Length=229, Percent_Identity=31.0043668122271, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1787031, Length=252, Percent_Identity=32.5396825396825, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28360; Mature: 28360

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVMFGALALSMLSLTAVAEDAKPIRIGIEAGYPPFSMKTPDGKLAGFDVDIGDALCE
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHH
QMKVKCTWVEQEFDGLIPALKVKKIDAILSSMTITDDRKKNVDFTIKYYHTPARFVMKAG
HHEEEEEEECHHHCCCCCHHHHHHHHHHHHHCEECCCCCCCCCEEEEEEECHHHHHHHCC
SGVKDPLTELKGKKVGVLRASTHDRYATEVLVPAGIELVRYGSQQEANLDMVSGRIDAML
CCCCCHHHHHCCCEEEEEEECCCCCHHHEEEECCCEEEEECCCCCCCCHHHHCCHHEEEE
ADSVNLSDGFLKTDAGKGFEFVGPTYEDAKYFGGGAGIAVRKGDTELAEKFNTAINEIRA
ECCCCCCCCEEECCCCCCEEECCCCHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHC
NGKYKQVQDKYFDFDVYGH
CCCHHHHHHHHCCEEECCC
>Mature Secondary Structure
MKKLVMFGALALSMLSLTAVAEDAKPIRIGIEAGYPPFSMKTPDGKLAGFDVDIGDALCE
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHH
QMKVKCTWVEQEFDGLIPALKVKKIDAILSSMTITDDRKKNVDFTIKYYHTPARFVMKAG
HHEEEEEEECHHHCCCCCHHHHHHHHHHHHHCEECCCCCCCCCEEEEEEECHHHHHHHCC
SGVKDPLTELKGKKVGVLRASTHDRYATEVLVPAGIELVRYGSQQEANLDMVSGRIDAML
CCCCCHHHHHCCCEEEEEEECCCCCHHHEEEECCCEEEEECCCCCCCCHHHHCCHHEEEE
ADSVNLSDGFLKTDAGKGFEFVGPTYEDAKYFGGGAGIAVRKGDTELAEKFNTAINEIRA
ECCCCCCCCEEECCCCCCEEECCCCHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHC
NGKYKQVQDKYFDFDVYGH
CCCHHHHHHHHCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]