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The map label for this gene is zraS [H]

Identifier: 77458227

GI number: 77458227

Start: 2280268

End: 2281716

Strand: Direct

Name: zraS [H]

Synonym: Pfl01_2000

Alternate gene names: 77458227

Gene position: 2280268-2281716 (Clockwise)

Preceding gene: 77458222

Following gene: 77458228

Centisome position: 35.42

GC content: 61.01

Gene sequence:

>1449_bases
ATGAACATGCAGTCACAATCGCTACCGGTCGAGGAGGGCAGCCAGCGGCTGAACTCGCGCAAGCAGCCGTTCAACCTGTT
GCGCTGGTTTTCATTGATCAGCATGGCGGTGATCGGCACCGTGGCGGTGGCGCTCGGGGCGGTGTCGACACGGTTCGTGA
TCGAAGAAAGCGTGCAGCGCGATGCCTTGCTCACTGCGCAGTTCATTCAGGCCATCGCGTCGGCGGAAGTGCGCCACGTT
TCGATCCCCAATATCCGCACCATGGGGGAGTTGCTCGACCCTCGTCAGGACAATGACTTTCCCGATGTCGACCCGCAGGC
CCGGGCCAGCGCCAGAGGTGAGTTTCTTGATCACATCGAGCATTTGCCGGATGTGATTCTCGCCAACGTTTATGCGCCGG
ATCGTACCGTGATCTGGTCGACCAATCCCGCGCTGGTCAACACTCGCATTCATGCCGACGAAGATCTTGACCGGGCGTTC
GATCAAAGGATCCCGGTGTCGGCGAGTTACCACAACGTAGACAAGGGCCGTGAAGAACAGAAGTTCGTGGTGCCGCCGGA
ATACATCTTCATCGAAAACTACATTCCACTGTTCGACGCCGAGGGCAAGAACGTCACGGCGATGGTCGAGATCTATAAGG
AGCCCAAGGACCTGATCGCCCGCATGGAGCGCGGTCTGGCGCTGATCTGGCTGGCCACCGCGCTGGGCGGCGGGCTGATT
TACCTGGGGTTGTACTGGATCGTGCGTCGGGCGGCGATTCTGCTGGCAGCCCAGCAGAAACAACTGATCGCCAACGAAAC
CTTTGTCGCGCTGGGCGAGATGTCTTCGGCGGTGGCCCACAGTCTGCGCAACCCGCTGGCGACCATTCGCTCGAGCGCCG
AACTGGCCCTGGAATTCGACGCCGGGCCGGCGCAGAAGAACATCAAGGACATCATCGGCCAGGTCGATCGCATGTCGAAG
TGGGTGCGCGAACTGCTGCAATCGCTGCGACCGCTCAATGACGACCCGGAACCGGTGAACCTGGTGGCGGCGCTGCACGA
CAGCCTGATGGCGTTCGAGCAGCAGATTGCCAAGGCTGGTGTGAACGTGGTGTTTCAACCTCAGCAGACGCCGATGGTGC
TGAGCCATCCGGTGCAGCTCACACAAATCCTCAACAGCCTGCTGGCCAACGCGCTGGAAGCGATGGACAAGGGCGGCACA
CTGACCATCACCCTCGAACCGAGCGACAGCCGTGGCGTGAGCGTCATGCTCAGCGATACCGGCAAAGGCATGAGCGAAGA
GCAGCGCAGCATGGCGTTCCGGCCGTTCTTCACCACCAAACAGGGTGGCCTCGGCGTGGGGCTGGTGCTGGTCAAACGGA
TCATGGAGCGCTTCGGCGGAGGCGTAAGCCTCGACAGCCGGGAAGGCAAGGGCACCACCGTTCGTCTGGCGTTTCAACTC
ATTCAGTAA

Upstream 100 bases:

>100_bases
CGGGAAATCGGCTGAGTGTGGGGCCGTTCACCCCCCGCATCCGGGCAAACACCCAACCGTGGGCTATAACCCTATAGGGA
CGTATCGGGAAGTCGCCGCC

Downstream 100 bases:

>100_bases
ATTGGGGTGAACTTCTCCCCAGCAGTGGGTGTCAGGCCCCAGTCACCGGGGAGTGGGGCATGCAACAGCACTCGCAAGCC
CCTGTAACAACTGGAAATGA

Product: periplasmic sensor Signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MNMQSQSLPVEEGSQRLNSRKQPFNLLRWFSLISMAVIGTVAVALGAVSTRFVIEESVQRDALLTAQFIQAIASAEVRHV
SIPNIRTMGELLDPRQDNDFPDVDPQARASARGEFLDHIEHLPDVILANVYAPDRTVIWSTNPALVNTRIHADEDLDRAF
DQRIPVSASYHNVDKGREEQKFVVPPEYIFIENYIPLFDAEGKNVTAMVEIYKEPKDLIARMERGLALIWLATALGGGLI
YLGLYWIVRRAAILLAAQQKQLIANETFVALGEMSSAVAHSLRNPLATIRSSAELALEFDAGPAQKNIKDIIGQVDRMSK
WVRELLQSLRPLNDDPEPVNLVAALHDSLMAFEQQIAKAGVNVVFQPQQTPMVLSHPVQLTQILNSLLANALEAMDKGGT
LTITLEPSDSRGVSVMLSDTGKGMSEEQRSMAFRPFFTTKQGGLGVGLVLVKRIMERFGGGVSLDSREGKGTTVRLAFQL
IQ

Sequences:

>Translated_482_residues
MNMQSQSLPVEEGSQRLNSRKQPFNLLRWFSLISMAVIGTVAVALGAVSTRFVIEESVQRDALLTAQFIQAIASAEVRHV
SIPNIRTMGELLDPRQDNDFPDVDPQARASARGEFLDHIEHLPDVILANVYAPDRTVIWSTNPALVNTRIHADEDLDRAF
DQRIPVSASYHNVDKGREEQKFVVPPEYIFIENYIPLFDAEGKNVTAMVEIYKEPKDLIARMERGLALIWLATALGGGLI
YLGLYWIVRRAAILLAAQQKQLIANETFVALGEMSSAVAHSLRNPLATIRSSAELALEFDAGPAQKNIKDIIGQVDRMSK
WVRELLQSLRPLNDDPEPVNLVAALHDSLMAFEQQIAKAGVNVVFQPQQTPMVLSHPVQLTQILNSLLANALEAMDKGGT
LTITLEPSDSRGVSVMLSDTGKGMSEEQRSMAFRPFFTTKQGGLGVGLVLVKRIMERFGGGVSLDSREGKGTTVRLAFQL
IQ
>Mature_482_residues
MNMQSQSLPVEEGSQRLNSRKQPFNLLRWFSLISMAVIGTVAVALGAVSTRFVIEESVQRDALLTAQFIQAIASAEVRHV
SIPNIRTMGELLDPRQDNDFPDVDPQARASARGEFLDHIEHLPDVILANVYAPDRTVIWSTNPALVNTRIHADEDLDRAF
DQRIPVSASYHNVDKGREEQKFVVPPEYIFIENYIPLFDAEGKNVTAMVEIYKEPKDLIARMERGLALIWLATALGGGLI
YLGLYWIVRRAAILLAAQQKQLIANETFVALGEMSSAVAHSLRNPLATIRSSAELALEFDAGPAQKNIKDIIGQVDRMSK
WVRELLQSLRPLNDDPEPVNLVAALHDSLMAFEQQIAKAGVNVVFQPQQTPMVLSHPVQLTQILNSLLANALEAMDKGGT
LTITLEPSDSRGVSVMLSDTGKGMSEEQRSMAFRPFFTTKQGGLGVGLVLVKRIMERFGGGVSLDSREGKGTTVRLAFQL
IQ

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=254, Percent_Identity=33.4645669291339, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1788549, Length=222, Percent_Identity=29.2792792792793, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1788393, Length=195, Percent_Identity=27.1794871794872, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1789808, Length=236, Percent_Identity=27.1186440677966, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790861, Length=211, Percent_Identity=28.436018957346, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87082128, Length=212, Percent_Identity=24.5283018867925, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53245; Mature: 53245

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMQSQSLPVEEGSQRLNSRKQPFNLLRWFSLISMAVIGTVAVALGAVSTRFVIEESVQR
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DALLTAQFIQAIASAEVRHVSIPNIRTMGELLDPRQDNDFPDVDPQARASARGEFLDHIE
HHHHHHHHHHHHHHCCEEEECCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
HLPDVILANVYAPDRTVIWSTNPALVNTRIHADEDLDRAFDQRIPVSASYHNVDKGREEQ
HHHHHHHHHCCCCCCEEEEECCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
KFVVPPEYIFIENYIPLFDAEGKNVTAMVEIYKEPKDLIARMERGLALIWLATALGGGLI
EEECCCCEEEEECCCCEECCCCCCEEEHHHHHHCHHHHHHHHHCCEEHHHHHHHHCCHHH
YLGLYWIVRRAAILLAAQQKQLIANETFVALGEMSSAVAHSLRNPLATIRSSAELALEFD
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
AGPAQKNIKDIIGQVDRMSKWVRELLQSLRPLNDDPEPVNLVAALHDSLMAFEQQIAKAG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
VNVVFQPQQTPMVLSHPVQLTQILNSLLANALEAMDKGGTLTITLEPSDSRGVSVMLSDT
CCEEECCCCCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECC
GKGMSEEQRSMAFRPFFTTKQGGLGVGLVLVKRIMERFGGGVSLDSREGKGTTVRLAFQL
CCCCCHHHHHCEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCEEEEEEEE
IQ
CC
>Mature Secondary Structure
MNMQSQSLPVEEGSQRLNSRKQPFNLLRWFSLISMAVIGTVAVALGAVSTRFVIEESVQR
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DALLTAQFIQAIASAEVRHVSIPNIRTMGELLDPRQDNDFPDVDPQARASARGEFLDHIE
HHHHHHHHHHHHHHCCEEEECCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
HLPDVILANVYAPDRTVIWSTNPALVNTRIHADEDLDRAFDQRIPVSASYHNVDKGREEQ
HHHHHHHHHCCCCCCEEEEECCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
KFVVPPEYIFIENYIPLFDAEGKNVTAMVEIYKEPKDLIARMERGLALIWLATALGGGLI
EEECCCCEEEEECCCCEECCCCCCEEEHHHHHHCHHHHHHHHHCCEEHHHHHHHHCCHHH
YLGLYWIVRRAAILLAAQQKQLIANETFVALGEMSSAVAHSLRNPLATIRSSAELALEFD
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
AGPAQKNIKDIIGQVDRMSKWVRELLQSLRPLNDDPEPVNLVAALHDSLMAFEQQIAKAG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
VNVVFQPQQTPMVLSHPVQLTQILNSLLANALEAMDKGGTLTITLEPSDSRGVSVMLSDT
CCEEECCCCCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECC
GKGMSEEQRSMAFRPFFTTKQGGLGVGLVLVKRIMERFGGGVSLDSREGKGTTVRLAFQL
CCCCCHHHHHCEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCEEEEEEEE
IQ
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]