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The map label for this gene is cefD [H]

Identifier: 77458079

GI number: 77458079

Start: 2107884

End: 2109179

Strand: Reverse

Name: cefD [H]

Synonym: Pfl01_1852

Alternate gene names: 77458079

Gene position: 2109179-2107884 (Counterclockwise)

Preceding gene: 77458080

Following gene: 77458078

Centisome position: 32.76

GC content: 62.42

Gene sequence:

>1296_bases
ATGAGTGATCGTCGTACGTTTCTCAAGCAGGCGGGGATTCTCGCCGCCGGCCTGCCGCTGGCCTCGGGCATCGCCCACGG
CGCCAGCACCGACAACACCACGAATGTTGCGCCAGCGGCGCCGGCACAGGACAAGTGGACGCAATTACGCACACTGTTCG
AGCAGGATCCCGACTGCCTGCACTTCTCCAACTTCCTGATTACCTCGCACCCCAGACCGGTGCGTGACGCCATCGCCCTG
CACCGCGCCGCCCTCGACAAGAACCCGGGGCTGGCGATGGACTGGGACCTGGGCGTGATCGAGCAGCGCGAGGAAGAGGT
ACGGGTCTGGGCCGGGCGTTATCTGCAAGCCGACGCCAGACAGATCGCCCTGACCGGCAGCACCACCGAGGGCCTGGCGA
TGATTTACGGCGGCGCCCATGTGCGTGCCGATCAGGAAATCCTCACCACTCAACACGAACACTACGCCACCCACACCATC
CTCGCGCTGCGCACACAGCGTGAAGGCACCAAGGTGCGCAAGATCCGTCTGTTCAAGGATCCGCACACGGCGACCAAGGC
CGAAATCCTGGCGGCGATCGACAGCAACATCCGCCCGGAAACCCGTGTATTGGGCATGTGCTGGGTGCATTCGGGCAGCG
GCGTGAAACTGCCGATCGGCGAAATCGGCGCGCTGGTCGACAAGCACAACAAGGGCCGTTCGGACGACAACCGGATCATT
TATCTGGTGGACGGCGTGCACGGTTTCGGCGTCGATGACCTGAGTTTCCCGGCCATGAACTGCGACTTCTTTATCGCCGG
CACCCACAAATGGATGTTCGGCCCACGCGGCACCGGCATCGTCTGCAGCCGTTTCGACCAGGTCAGGTACATCACGCCGA
TCATTCCGACCTTCTCCGAAGCCACCGCGTTTTCCACCACCATGACCCCCGGCGGCTATCACGCCTTCGAGCACCGCTGG
GCCTTGAACGAAGCGTTCAAATTGCACCTGCAACTGGGCAAGGCCGACGTCCAGGCGCGTATTCACGGCTTGAACAGCTA
TCTGAAAAAGCGCCTGCAGGAACATCCGCAGATCGAACTGGTGACGCCGCTGAGCCCCGAACTCTCCGCCGGTTTCACCT
TCTTCCGGGTCAAGGGCAAGGACTGCGACAAGATCGCCGCCTACCTGATGCAGAACAAGGTGATCGCCGATGCCGTCGAC
CGCGACGTCGGCCCGGTCATTCGCAGCGCGCCGGGCCTGTTGAACACCGAACAAGAAATCGACCGTTTCATGACCGTGCT
CGTCAGCCAGATCTGA

Upstream 100 bases:

>100_bases
ACATCGCCAAGCTCTGGGGCGGCAACTTCCTGCGGGTCTGGGATCAGGTGCAGAAAGCCGCCCGACCTGCGCTGATTTCA
CAGCAGGACAAGATCAAGCC

Downstream 100 bases:

>100_bases
GCACCGTGAACGCCTTTAAACCTGTTATCGAGAGAAATGATGAACAGTGAGCTACGTAGTTTTTCCATCAAGTCGCTCCC
CGCGCTGACCCTGGCCGCCC

Product: twin-arginine translocation pathway signal protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MSDRRTFLKQAGILAAGLPLASGIAHGASTDNTTNVAPAAPAQDKWTQLRTLFEQDPDCLHFSNFLITSHPRPVRDAIAL
HRAALDKNPGLAMDWDLGVIEQREEEVRVWAGRYLQADARQIALTGSTTEGLAMIYGGAHVRADQEILTTQHEHYATHTI
LALRTQREGTKVRKIRLFKDPHTATKAEILAAIDSNIRPETRVLGMCWVHSGSGVKLPIGEIGALVDKHNKGRSDDNRII
YLVDGVHGFGVDDLSFPAMNCDFFIAGTHKWMFGPRGTGIVCSRFDQVRYITPIIPTFSEATAFSTTMTPGGYHAFEHRW
ALNEAFKLHLQLGKADVQARIHGLNSYLKKRLQEHPQIELVTPLSPELSAGFTFFRVKGKDCDKIAAYLMQNKVIADAVD
RDVGPVIRSAPGLLNTEQEIDRFMTVLVSQI

Sequences:

>Translated_431_residues
MSDRRTFLKQAGILAAGLPLASGIAHGASTDNTTNVAPAAPAQDKWTQLRTLFEQDPDCLHFSNFLITSHPRPVRDAIAL
HRAALDKNPGLAMDWDLGVIEQREEEVRVWAGRYLQADARQIALTGSTTEGLAMIYGGAHVRADQEILTTQHEHYATHTI
LALRTQREGTKVRKIRLFKDPHTATKAEILAAIDSNIRPETRVLGMCWVHSGSGVKLPIGEIGALVDKHNKGRSDDNRII
YLVDGVHGFGVDDLSFPAMNCDFFIAGTHKWMFGPRGTGIVCSRFDQVRYITPIIPTFSEATAFSTTMTPGGYHAFEHRW
ALNEAFKLHLQLGKADVQARIHGLNSYLKKRLQEHPQIELVTPLSPELSAGFTFFRVKGKDCDKIAAYLMQNKVIADAVD
RDVGPVIRSAPGLLNTEQEIDRFMTVLVSQI
>Mature_430_residues
SDRRTFLKQAGILAAGLPLASGIAHGASTDNTTNVAPAAPAQDKWTQLRTLFEQDPDCLHFSNFLITSHPRPVRDAIALH
RAALDKNPGLAMDWDLGVIEQREEEVRVWAGRYLQADARQIALTGSTTEGLAMIYGGAHVRADQEILTTQHEHYATHTIL
ALRTQREGTKVRKIRLFKDPHTATKAEILAAIDSNIRPETRVLGMCWVHSGSGVKLPIGEIGALVDKHNKGRSDDNRIIY
LVDGVHGFGVDDLSFPAMNCDFFIAGTHKWMFGPRGTGIVCSRFDQVRYITPIIPTFSEATAFSTTMTPGGYHAFEHRWA
LNEAFKLHLQLGKADVQARIHGLNSYLKKRLQEHPQIELVTPLSPELSAGFTFFRVKGKDCDKIAAYLMQNKVIADAVDR
DVGPVIRSAPGLLNTEQEIDRFMTVLVSQI

Specific function: Catalyzes the reversible isomerization between isopenicillin N and penicillin N [H]

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI1787970, Length=346, Percent_Identity=23.4104046242775, Blast_Score=76, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =5.1.1.17 [H]

Molecular weight: Translated: 47762; Mature: 47631

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRRTFLKQAGILAAGLPLASGIAHGASTDNTTNVAPAAPAQDKWTQLRTLFEQDPDCL
CCCHHHHHHHCCHHEECCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEE
HFSNFLITSHPRPVRDAIALHRAALDKNPGLAMDWDLGVIEQREEEVRVWAGRYLQADAR
EECCEEEECCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHCCHHHHHHHHHHHEECCCE
QIALTGSTTEGLAMIYGGAHVRADQEILTTQHEHYATHTILALRTQREGTKVRKIRLFKD
EEEEECCCCCCEEEEECCCEEECCHHHHHCCHHHHHHEEEEEEEECCCCCEEEEEEEECC
PHTATKAEILAAIDSNIRPETRVLGMCWVHSGSGVKLPIGEIGALVDKHNKGRSDDNRII
CCCHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCEECHHHHHHHHHCCCCCCCCCCEEE
YLVDGVHGFGVDDLSFPAMNCDFFIAGTHKWMFGPRGTGIVCSRFDQVRYITPIIPTFSE
EEEECCCCCCCCCCCCCCCCCEEEEEECCEEEECCCCCCCEEECCCCCEEECCCCCCCCC
ATAFSTTMTPGGYHAFEHRWALNEAFKLHLQLGKADVQARIHGLNSYLKKRLQEHPQIEL
CCEEEEECCCCCCHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEE
VTPLSPELSAGFTFFRVKGKDCDKIAAYLMQNKVIADAVDRDVGPVIRSAPGLLNTEQEI
ECCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCHHHHHHCCHHHHHHHCCCCCCCCHHHH
DRFMTVLVSQI
HHHHHHHHHCC
>Mature Secondary Structure 
SDRRTFLKQAGILAAGLPLASGIAHGASTDNTTNVAPAAPAQDKWTQLRTLFEQDPDCL
CCHHHHHHHCCHHEECCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEE
HFSNFLITSHPRPVRDAIALHRAALDKNPGLAMDWDLGVIEQREEEVRVWAGRYLQADAR
EECCEEEECCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHCCHHHHHHHHHHHEECCCE
QIALTGSTTEGLAMIYGGAHVRADQEILTTQHEHYATHTILALRTQREGTKVRKIRLFKD
EEEEECCCCCCEEEEECCCEEECCHHHHHCCHHHHHHEEEEEEEECCCCCEEEEEEEECC
PHTATKAEILAAIDSNIRPETRVLGMCWVHSGSGVKLPIGEIGALVDKHNKGRSDDNRII
CCCHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCEECHHHHHHHHHCCCCCCCCCCEEE
YLVDGVHGFGVDDLSFPAMNCDFFIAGTHKWMFGPRGTGIVCSRFDQVRYITPIIPTFSE
EEEECCCCCCCCCCCCCCCCCEEEEEECCEEEECCCCCCCEEECCCCCEEECCCCCCCCC
ATAFSTTMTPGGYHAFEHRWALNEAFKLHLQLGKADVQARIHGLNSYLKKRLQEHPQIEL
CCEEEEECCCCCCHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEE
VTPLSPELSAGFTFFRVKGKDCDKIAAYLMQNKVIADAVDRDVGPVIRSAPGLLNTEQEI
ECCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCHHHHHHCCHHHHHHHCCCCCCCCHHHH
DRFMTVLVSQI
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1694525; 2804141 [H]