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The map label for this gene is guaD [H]

Identifier: 77458021

GI number: 77458021

Start: 1998809

End: 2000113

Strand: Reverse

Name: guaD [H]

Synonym: Pfl01_1794

Alternate gene names: 77458021

Gene position: 2000113-1998809 (Counterclockwise)

Preceding gene: 77458022

Following gene: 77458019

Centisome position: 31.07

GC content: 63.14

Gene sequence:

>1305_bases
ATGCCTCTGACTCGCAAAGCCTACCGCGCCGCCATCCTGCACAGCATCGCCGACCCTGCCGAGGTCGGCATCGAAGCCTC
CTACGAGTATTTCGAGGACGGCCTGCTGGTGGTCGATGACGGCAAGATCAGCGCCCTCGGCCACGCCAGTGAACTGCTGC
CGACACTGCCGGCGGACATCGAGATCACCGATCACAAGGATGCGCTGATCACTCCGGGCTTCATCGACACCCACATCCAC
CTTCCGCAGACCGGCATGGTCGGCGCCTATGGTGAGCAACTGCTGGACTGGCTGAACACCTACACCTTCCCGTGCGAAAG
CCAGTTTGCCGACAAGGCCCACGCCGATGAAGTGGCGGACATTTTCATCAAGGAACTGCTGCGCAACGGCACCACCACCG
CGCTGGTGTTCGGCAGCGTGCATCCGCAGTCGGTGAACTCGTTCTTCGAAGCGGCCGAGAAGCTGGACCTGCGGATGATC
GCCGGCAAGGTGATGATGGACCGCAACGCGCCGGACTACCTGACCGACACTCCGGAATCGAGCTACGTCGACAGCAAGGC
GCTGATCGAGCGCTGGCACGGCAAGGGTCGTCTGCACTATGCGGTCACCCCGCGTTTCGCCCCGACCAGCACCCCGGAGC
AGCTGACACTCGCCGGCCAGCTGCTCAGCGAATACCCGGATCTGTACATGCAGACCCACATCAGCGAAAACCTCAAGGAA
GTCGAGTGGGTCAAGGCGCTGTTCCCGGAGCGCAAAGGCTATCTGGACGTCTACGATCACTACCAATTGCTCGGCGAGCG
CTCGGTGTTCGCCCACGGCGTGCACCTGTGCGACGACGAATGCGCGCGCCTGGCGCAGACCGGTTCTGCGGTCGCGTTTT
GCCCGACCTCGAACTTCTTCCTCGGCAGCGGCCTGTTCAACCTGCCGATGGCCGAGAAGCACAAACTGAACGTCGGCCTC
GGGACTGACGTCGGCGGCGGCACCAGTTTCTCGCTGCTGCAAACCCTGAACGAAGCCTACAAGGTGATGCAGTTGCAAGG
CGCACGCCTGAGCCCGTTCAAGTCGCTGTACCTCGCCACCCTCGGCGGTGCCCGGGCGCTGCGCCTGGAAGACAAGATCG
GCAACCTGCAACCGGGCTCCGACGCCGACTTCCTGGTGCTGGACTACAACGCCACGCCGCTGCTGAGCTACCGCTTGAAG
CAGGCCAACAATATTGCCGAGACGTTGTTTGTGCTGATGACCCTGGGCGATGACCGCACTGTGGCCCAGACCTATGCGGC
GGGTGCGCTGGTGCACCAGCGCTAG

Upstream 100 bases:

>100_bases
CAGCACACCGCCAGTGCCGAACCGATTGCCAAACTGCTGCCGGTTTCGCGCCGCAGTCAGGCCGCCACACTCAAAGCCTC
AAACTGATTAGAGAACCCTC

Downstream 100 bases:

>100_bases
TTTTCCGCAGACAAAAAAAATCCCCCGGTACTTTGCAGTCCGGGGGATTTTTATTGCCTGTCGATTCGCTTCAGAGCTTG
ACCGCCGGCCGCCCCGGTTT

Product: guanine deaminase

Products: NA

Alternate protein names: Guanase; Guanine aminase; Guanine aminohydrolase; GAH [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MPLTRKAYRAAILHSIADPAEVGIEASYEYFEDGLLVVDDGKISALGHASELLPTLPADIEITDHKDALITPGFIDTHIH
LPQTGMVGAYGEQLLDWLNTYTFPCESQFADKAHADEVADIFIKELLRNGTTTALVFGSVHPQSVNSFFEAAEKLDLRMI
AGKVMMDRNAPDYLTDTPESSYVDSKALIERWHGKGRLHYAVTPRFAPTSTPEQLTLAGQLLSEYPDLYMQTHISENLKE
VEWVKALFPERKGYLDVYDHYQLLGERSVFAHGVHLCDDECARLAQTGSAVAFCPTSNFFLGSGLFNLPMAEKHKLNVGL
GTDVGGGTSFSLLQTLNEAYKVMQLQGARLSPFKSLYLATLGGARALRLEDKIGNLQPGSDADFLVLDYNATPLLSYRLK
QANNIAETLFVLMTLGDDRTVAQTYAAGALVHQR

Sequences:

>Translated_434_residues
MPLTRKAYRAAILHSIADPAEVGIEASYEYFEDGLLVVDDGKISALGHASELLPTLPADIEITDHKDALITPGFIDTHIH
LPQTGMVGAYGEQLLDWLNTYTFPCESQFADKAHADEVADIFIKELLRNGTTTALVFGSVHPQSVNSFFEAAEKLDLRMI
AGKVMMDRNAPDYLTDTPESSYVDSKALIERWHGKGRLHYAVTPRFAPTSTPEQLTLAGQLLSEYPDLYMQTHISENLKE
VEWVKALFPERKGYLDVYDHYQLLGERSVFAHGVHLCDDECARLAQTGSAVAFCPTSNFFLGSGLFNLPMAEKHKLNVGL
GTDVGGGTSFSLLQTLNEAYKVMQLQGARLSPFKSLYLATLGGARALRLEDKIGNLQPGSDADFLVLDYNATPLLSYRLK
QANNIAETLFVLMTLGDDRTVAQTYAAGALVHQR
>Mature_433_residues
PLTRKAYRAAILHSIADPAEVGIEASYEYFEDGLLVVDDGKISALGHASELLPTLPADIEITDHKDALITPGFIDTHIHL
PQTGMVGAYGEQLLDWLNTYTFPCESQFADKAHADEVADIFIKELLRNGTTTALVFGSVHPQSVNSFFEAAEKLDLRMIA
GKVMMDRNAPDYLTDTPESSYVDSKALIERWHGKGRLHYAVTPRFAPTSTPEQLTLAGQLLSEYPDLYMQTHISENLKEV
EWVKALFPERKGYLDVYDHYQLLGERSVFAHGVHLCDDECARLAQTGSAVAFCPTSNFFLGSGLFNLPMAEKHKLNVGLG
TDVGGGTSFSLLQTLNEAYKVMQLQGARLSPFKSLYLATLGGARALRLEDKIGNLQPGSDADFLVLDYNATPLLSYRLKQ
ANNIAETLFVLMTLGDDRTVAQTYAAGALVHQR

Specific function: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia [H]

COG id: COG0402

COG function: function code FR; Cytosine deaminase and related metal-dependent hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATZ/TRZ family [H]

Homologues:

Organism=Homo sapiens, GI4758426, Length=455, Percent_Identity=35.1648351648352, Blast_Score=227, Evalue=1e-59,
Organism=Escherichia coli, GI1789249, Length=434, Percent_Identity=56.4516129032258, Blast_Score=518, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI17540282, Length=399, Percent_Identity=23.8095238095238, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319963, Length=434, Percent_Identity=32.9493087557604, Blast_Score=223, Evalue=5e-59,
Organism=Drosophila melanogaster, GI24643849, Length=441, Percent_Identity=36.281179138322, Blast_Score=238, Evalue=5e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR014311
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.4.3 [H]

Molecular weight: Translated: 47822; Mature: 47691

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTRKAYRAAILHSIADPAEVGIEASYEYFEDGLLVVDDGKISALGHASELLPTLPADI
CCCCHHHHHHHHHHHHCCHHHHCCCCCHHHHHCCEEEEECCCEEECCCHHHHHHCCCCCE
EITDHKDALITPGFIDTHIHLPQTGMVGAYGEQLLDWLNTYTFPCESQFADKAHADEVAD
EEECCCCCEECCCEEECEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
IFIKELLRNGTTTALVFGSVHPQSVNSFFEAAEKLDLRMIAGKVMMDRNAPDYLTDTPES
HHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCC
SYVDSKALIERWHGKGRLHYAVTPRFAPTSTPEQLTLAGQLLSEYPDLYMQTHISENLKE
CCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHH
VEWVKALFPERKGYLDVYDHYQLLGERSVFAHGVHLCDDECARLAQTGSAVAFCPTSNFF
HHHHHHHCCCCCCCEEHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHCCCEEEECCCCCCE
LGSGLFNLPMAEKHKLNVGLGTDVGGGTSFSLLQTLNEAYKVMQLQGARLSPFKSLYLAT
ECCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LGGARALRLEDKIGNLQPGSDADFLVLDYNATPLLSYRLKQANNIAETLFVLMTLGDDRT
CCCCEEEEEHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHEECCCCCH
VAQTYAAGALVHQR
HHHHHHHHHHHCCC
>Mature Secondary Structure 
PLTRKAYRAAILHSIADPAEVGIEASYEYFEDGLLVVDDGKISALGHASELLPTLPADI
CCCHHHHHHHHHHHHCCHHHHCCCCCHHHHHCCEEEEECCCEEECCCHHHHHHCCCCCE
EITDHKDALITPGFIDTHIHLPQTGMVGAYGEQLLDWLNTYTFPCESQFADKAHADEVAD
EEECCCCCEECCCEEECEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
IFIKELLRNGTTTALVFGSVHPQSVNSFFEAAEKLDLRMIAGKVMMDRNAPDYLTDTPES
HHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCC
SYVDSKALIERWHGKGRLHYAVTPRFAPTSTPEQLTLAGQLLSEYPDLYMQTHISENLKE
CCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHH
VEWVKALFPERKGYLDVYDHYQLLGERSVFAHGVHLCDDECARLAQTGSAVAFCPTSNFF
HHHHHHHCCCCCCCEEHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHCCCEEEECCCCCCE
LGSGLFNLPMAEKHKLNVGLGTDVGGGTSFSLLQTLNEAYKVMQLQGARLSPFKSLYLAT
ECCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LGGARALRLEDKIGNLQPGSDADFLVLDYNATPLLSYRLKQANNIAETLFVLMTLGDDRT
CCCCEEEEEHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHEECCCCCH
VAQTYAAGALVHQR
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 10913105 [H]