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The map label for this gene is oprF [H]

Identifier: 77458004

GI number: 77458004

Start: 1974816

End: 1975850

Strand: Direct

Name: oprF [H]

Synonym: Pfl01_1777

Alternate gene names: 77458004

Gene position: 1974816-1975850 (Clockwise)

Preceding gene: 77458003

Following gene: 77458013

Centisome position: 30.67

GC content: 55.75

Gene sequence:

>1035_bases
ATGAAACTGAAAAACACCTTGGGCTTGGCCATTGGTTCTTTGATTGCCGCCACTTCTTTCGGCGCTCTGGCACAAGGCCA
AGGCGCAGTTGAAATCGAAGGCTTCGCAAAGAAAGAACAATTCGACAGCGCTCGTAACTTCAAGAACAACGGCAACCTGT
TCGGCGGTTCGATCGGTTACTTCCTGACCGACGACGTTGAACTGCGTCTGGGCTACGACGAAGTGCACAACGTGCGTGCC
GACGATGGCAAGAACGTCAAGGGCGCCAACACCGCTCTGGACGCTCTGTACCACTTCAACAACCCAGGCGACATGCTGCG
TCCATACGTTTCGGCCGGTTTCTCTGACCAGAGCATCGACCAGAACGGTTCGAACGGTCGCAACCGTTCCACCTTCGCCA
ACCTGGGCGCCGGTGCCAAGCTGTACTTCACTGACAACTTCTACGCCCGTGCTGGCGTTGAAGCTCAGTACAACATCGAC
CAGGGCGACACCGAGTGGGCTCCAAGCGTCGGTATCGGTGTGAACTTCGGTGGCGGCTCCAAGCCAGCCGCTGCTCCAGT
TCCAGCACCAGCTGAAGTCTGCTCCGACAGCGACAACGACGGCGTTTGCGACAACGTTGACAAGTGCCCGGACACCCCAG
CCAACGTAACTGTTGACGCTGATGGCTGCCCTGCAGTTGCTGAAGTTGTTCGTGTTGAGCTGGACGTTAAGTTCGACTTC
GACAAGTCGGTTGTGAAGCCAAACAGCTATGGCGACATCAAAAACCTGGCTGACTTCATGAAGCAGTACCCATCCACCAC
CACTACTGTTGAAGGTCACACTGACTCCGTCGGTCCTGACGCTTACAACCAGAAACTGTCCGAGCGTCGTGCAAACGCCG
TTAAGCAAGTTCTGACCAACCAGTACGGTGTTGAATCGTCCCGCGTTCAGTCGATCGGCTACGGCGAATCCCGCCCAGTT
GCTGACAACAAAACTGAAGCTGGCCGTGCTGTTAACCGTCGCGTAGAAGCGCAGGTTGAAGCTCAAGCTAAGTAA

Upstream 100 bases:

>100_bases
CAAATATCTCTTACGTGTAGGCAAGTTCTGATAGACTTGCCGCCGAGTTGTCCCCCGGTTTGCGGGACTGCTTCACAATC
ACCAGATGGGGATTTAACGG

Downstream 100 bases:

>100_bases
TTAGCTGCCGCTACGAGAAAAGCCCGGCTTAGGCCGGGCTTTTCTTTGCCTGCGGTTTGGTGAATCCAGAGATTGTCAGG
CCAGGCGATCGGCTGCCCAG

Product: OmpF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MKLKNTLGLAIGSLIAATSFGALAQGQGAVEIEGFAKKEQFDSARNFKNNGNLFGGSIGYFLTDDVELRLGYDEVHNVRA
DDGKNVKGANTALDALYHFNNPGDMLRPYVSAGFSDQSIDQNGSNGRNRSTFANLGAGAKLYFTDNFYARAGVEAQYNID
QGDTEWAPSVGIGVNFGGGSKPAAAPVPAPAEVCSDSDNDGVCDNVDKCPDTPANVTVDADGCPAVAEVVRVELDVKFDF
DKSVVKPNSYGDIKNLADFMKQYPSTTTTVEGHTDSVGPDAYNQKLSERRANAVKQVLTNQYGVESSRVQSIGYGESRPV
ADNKTEAGRAVNRRVEAQVEAQAK

Sequences:

>Translated_344_residues
MKLKNTLGLAIGSLIAATSFGALAQGQGAVEIEGFAKKEQFDSARNFKNNGNLFGGSIGYFLTDDVELRLGYDEVHNVRA
DDGKNVKGANTALDALYHFNNPGDMLRPYVSAGFSDQSIDQNGSNGRNRSTFANLGAGAKLYFTDNFYARAGVEAQYNID
QGDTEWAPSVGIGVNFGGGSKPAAAPVPAPAEVCSDSDNDGVCDNVDKCPDTPANVTVDADGCPAVAEVVRVELDVKFDF
DKSVVKPNSYGDIKNLADFMKQYPSTTTTVEGHTDSVGPDAYNQKLSERRANAVKQVLTNQYGVESSRVQSIGYGESRPV
ADNKTEAGRAVNRRVEAQVEAQAK
>Mature_344_residues
MKLKNTLGLAIGSLIAATSFGALAQGQGAVEIEGFAKKEQFDSARNFKNNGNLFGGSIGYFLTDDVELRLGYDEVHNVRA
DDGKNVKGANTALDALYHFNNPGDMLRPYVSAGFSDQSIDQNGSNGRNRSTFANLGAGAKLYFTDNFYARAGVEAQYNID
QGDTEWAPSVGIGVNFGGGSKPAAAPVPAPAEVCSDSDNDGVCDNVDKCPDTPANVTVDADGCPAVAEVVRVELDVKFDF
DKSVVKPNSYGDIKNLADFMKQYPSTTTTVEGHTDSVGPDAYNQKLSERRANAVKQVLTNQYGVESSRVQSIGYGESRPV
ADNKTEAGRAVNRRVEAQVEAQAK

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=130, Percent_Identity=36.1538461538462, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1786962, Length=99, Percent_Identity=38.3838383838384, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787191, Length=117, Percent_Identity=34.1880341880342, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1788957, Length=89, Percent_Identity=38.2022471910112, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 36662; Mature: 36662

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS01068 OMPA_1 ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKNTLGLAIGSLIAATSFGALAQGQGAVEIEGFAKKEQFDSARNFKNNGNLFGGSIGY
CCCCHHHHHHHHHHHHHHHCCHHHCCCCCEEECCCHHHHHHHHHHCCCCCCCEECCEECE
FLTDDVELRLGYDEVHNVRADDGKNVKGANTALDALYHFNNPGDMLRPYVSAGFSDQSID
EEECCEEEEECHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCC
QNGSNGRNRSTFANLGAGAKLYFTDNFYARAGVEAQYNIDQGDTEWAPSVGIGVNFGGGS
CCCCCCCCCCHHHHCCCCEEEEEECCEEEECCCCEEECCCCCCCCCCCCCCEEEECCCCC
KPAAAPVPAPAEVCSDSDNDGVCDNVDKCPDTPANVTVDADGCPAVAEVVRVELDVKFDF
CCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHEEEEEEEEECC
DKSVVKPNSYGDIKNLADFMKQYPSTTTTVEGHTDSVGPDAYNQKLSERRANAVKQVLTN
CCCCCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QYGVESSRVQSIGYGESRPVADNKTEAGRAVNRRVEAQVEAQAK
HCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCC
>Mature Secondary Structure
MKLKNTLGLAIGSLIAATSFGALAQGQGAVEIEGFAKKEQFDSARNFKNNGNLFGGSIGY
CCCCHHHHHHHHHHHHHHHCCHHHCCCCCEEECCCHHHHHHHHHHCCCCCCCEECCEECE
FLTDDVELRLGYDEVHNVRADDGKNVKGANTALDALYHFNNPGDMLRPYVSAGFSDQSID
EEECCEEEEECHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCC
QNGSNGRNRSTFANLGAGAKLYFTDNFYARAGVEAQYNIDQGDTEWAPSVGIGVNFGGGS
CCCCCCCCCCHHHHCCCCEEEEEECCEEEECCCCEEECCCCCCCCCCCCCCEEEECCCCC
KPAAAPVPAPAEVCSDSDNDGVCDNVDKCPDTPANVTVDADGCPAVAEVVRVELDVKFDF
CCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHEEEEEEEEECC
DKSVVKPNSYGDIKNLADFMKQYPSTTTTVEGHTDSVGPDAYNQKLSERRANAVKQVLTN
CCCCCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QYGVESSRVQSIGYGESRPVADNKTEAGRAVNRRVEAQVEAQAK
HCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1898935 [H]