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The map label for this gene is ppsA [H]

Identifier: 77457997

GI number: 77457997

Start: 1967687

End: 1970062

Strand: Direct

Name: ppsA [H]

Synonym: Pfl01_1770

Alternate gene names: 77457997

Gene position: 1967687-1970062 (Clockwise)

Preceding gene: 77457995

Following gene: 77457998

Centisome position: 30.56

GC content: 61.83

Gene sequence:

>2376_bases
TTGGTAGAGTACGTAGTTTCCCTCGATAAGCTCGGCAAACACGATGTTGAGCATGTGGGGGGCAAGAACGCATCCCTGGG
CGAGATGATCAGTAACCTGGCCGGTGCCGGTGTTTCGGTCCCCGGCGGCTTCGCCACCACGGCGCAGGCCTATCGTGATT
TCCTGGAACTGAGCGGTCTGAACGATCAGATCCACAAGGCCCTCGATGCGCTGGACGTCGATGACGTCAATGCCCTGGCC
AAGACCGGCGCCCAGATCCGCCAATGGATCATGGAAGCCGAATTCCCTGAAAAACTGAACACCGAGATCCGCACCGCGTT
CGCCGCGCTGTCGGCCGGTAACCCTGACGTGGCCGTGGCCGTGCGTTCCTCCGCCACCGCCGAAGACTTGCCGGACGCTT
CGTTCGCCGGTCAGCAGGAAACCTTCCTGAACATCCGTGGCGTGGAAAACGTCATCCGCGCGGCCAAGGAAGTGTTCGCT
TCCCTGTTCAACGACCGCGCCATTTCCTACCGCGTGCACCAGGGCTTCGACCACAAGCTGGTCGCCCTGTCGGCCGGCGT
GCAGCGCATGGTGCGTTCGGAAACCGGCACCGCCGGCGTGATGTTCACCCTCGATACCGAATCCGGCTTCCGTGACGTGG
TGTTCATCACCGGCGCCTACGGCCTGGGCGAAACCGTCGTGCAAGGCGCGGTGAACCCGGACGAATTCTATGTCCACAAG
GGCACGCTGGAGGCCGGTCGCCCGGCGATCCTGCGTCGCAACCTGGGCAGCAAGGCCATCAAGATGATCTACGGCGACGA
GGCCAAGGCCGGTCGTTCCGTGAAAACCGTTGATGTCGACAAGGCCGAGCGCGCGCGTTTCTGCCTGACCGACGCTGAAG
TCAGCGAGCTGGCCAAGCAGGCGATGATCATCGAGAAGCATTATGGCTGCCCGATGGACATCGAGTGGGCCAAGGACGGT
GACGACGGCAAGCTGTACATCGTGCAGGCCCGTCCGGAAACCGTGAAAAGTCGCACCCAGGCCAACGTCATGGAACGTTA
CCTGCTGAAAGAAACCGGCACCGTGCTGGTGGAAGGTCGTGCCATCGGCCAGCGCATCGGCGCCGGCAAGGTGCGGATCA
TCAAGGACGTCTCCGAGATGGACAAGGTCCAGCCGGGAGACGTGCTGGTCTCCGACATGACCGACCCGGACTGGGAGCCG
GTCATGAAGCGCGCCAGCGCCATCGTCACCAACCGTGGTGGCCGTACCTGCCACGCGGCGATCATCGCCCGTGAGCTGGG
GATTCCTGCAGTCGTGGGTTGCGGCAACGCCACCCAACTGCTGAAGGATGGCCAAGGCGTGACCGTGTCCTGCGCCGAAG
GCGACACCGGTTACATCTTCGAAGGCGAACTGGGCTTCGACATCAAGAAGAACTCCGTCGACGCCATGCCGGAGCTGCCG
TTCAAGATCATGATGAACGTCGGCAACCCGGATCGCGCCTTCGACTTCGCGCAGCTGCCGAACGCCGGTGTGGGCCTGGC
CCGTCTGGAGTTCATCATCAACCGCATGATCGGCGTCCACCCGAAAGCGCTGTTGAACTACGACGGCCTGCCGCAGGACA
TCAAGGAAAGCGTCGACAAGCGCATCGCCGGTTACGACGATCCGGTCGGCTTCTATGTCGAGAAGCTGGTTGAAGGCATC
AGCACCCTGGCCGCTGCGTTTGCACCGAAGAAGGTCATCGTGCGTCTGTCGGACTTCAAGTCCAACGAGTACGCCAACCT
GATCGGCGGCAAGCTGTACGAGCCGGAAGAAGAAAACCCGATGCTGGGCTTCCGTGGCGCTTCGCGTTACATCAGTGAAT
CGTTCCGTGACTGCTTCGAACTCGAGTGCCGTGCGCTGAAACGCGTACGCAACGAGATGGGCCTGACCAACGTCGAAATC
ATGGTGCCGTTCGTCCGTACCCTCGGCGAAGCCAGCCAGGTGGTGGATCTGCTCGCCGAAAACGGCCTGGCCCGTGGTGA
CAACGGTCTGCGCGTGATCATGATGTGCGAACTGCCGTCCAACGCGATCCTGGCGGAAGAATTCCTCGAATTCTTCGATG
GTTTCTCGATCGGCTCCAACGACCTGACTCAGCTGACACTGGGTCTGGACCGTGACTCCGGGATCATCGCGCACCTGTTC
GACGAGCGTAATCCGGCGGTCAAGAAACTGCTGGCCAACGCGATTGCCGCGTGCAACAAGGCCGGCAAGTACATCGGCAT
CTGCGGTCAGGGTCCTTCGGACCACCCGGACCTGGCCAAGTGGCTGATGGAGCAGGGCATCGAAAGCGTGTCGCTGAACC
CGGACACCGTGCTGGAAACCTGGTTCTTCCTTGCCGAAGGCCAGGCGGCGGAATAA

Upstream 100 bases:

>100_bases
ATTCTCACTTATTTTCCAGGTCCAGGCCATACAGCCGGCCAACGCTCCCCCGAGCCGCCGGTTTCTGAGCTTTTCCAACA
CAGTTAGTGGAGAGATCACC

Downstream 100 bases:

>100_bases
GCCGTGAATGAAGCCCCGGCCCGTTGAACCGGGTCGGGGCTTTAAGATTGAAGTAGGGCGAGCTCTCCGGATGCCGCCCT
TTTTTGTGCAAGAGCATTAT

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 791; Mature: 791

Protein sequence:

>791_residues
MVEYVVSLDKLGKHDVEHVGGKNASLGEMISNLAGAGVSVPGGFATTAQAYRDFLELSGLNDQIHKALDALDVDDVNALA
KTGAQIRQWIMEAEFPEKLNTEIRTAFAALSAGNPDVAVAVRSSATAEDLPDASFAGQQETFLNIRGVENVIRAAKEVFA
SLFNDRAISYRVHQGFDHKLVALSAGVQRMVRSETGTAGVMFTLDTESGFRDVVFITGAYGLGETVVQGAVNPDEFYVHK
GTLEAGRPAILRRNLGSKAIKMIYGDEAKAGRSVKTVDVDKAERARFCLTDAEVSELAKQAMIIEKHYGCPMDIEWAKDG
DDGKLYIVQARPETVKSRTQANVMERYLLKETGTVLVEGRAIGQRIGAGKVRIIKDVSEMDKVQPGDVLVSDMTDPDWEP
VMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATQLLKDGQGVTVSCAEGDTGYIFEGELGFDIKKNSVDAMPELP
FKIMMNVGNPDRAFDFAQLPNAGVGLARLEFIINRMIGVHPKALLNYDGLPQDIKESVDKRIAGYDDPVGFYVEKLVEGI
STLAAAFAPKKVIVRLSDFKSNEYANLIGGKLYEPEEENPMLGFRGASRYISESFRDCFELECRALKRVRNEMGLTNVEI
MVPFVRTLGEASQVVDLLAENGLARGDNGLRVIMMCELPSNAILAEEFLEFFDGFSIGSNDLTQLTLGLDRDSGIIAHLF
DERNPAVKKLLANAIAACNKAGKYIGICGQGPSDHPDLAKWLMEQGIESVSLNPDTVLETWFFLAEGQAAE

Sequences:

>Translated_791_residues
MVEYVVSLDKLGKHDVEHVGGKNASLGEMISNLAGAGVSVPGGFATTAQAYRDFLELSGLNDQIHKALDALDVDDVNALA
KTGAQIRQWIMEAEFPEKLNTEIRTAFAALSAGNPDVAVAVRSSATAEDLPDASFAGQQETFLNIRGVENVIRAAKEVFA
SLFNDRAISYRVHQGFDHKLVALSAGVQRMVRSETGTAGVMFTLDTESGFRDVVFITGAYGLGETVVQGAVNPDEFYVHK
GTLEAGRPAILRRNLGSKAIKMIYGDEAKAGRSVKTVDVDKAERARFCLTDAEVSELAKQAMIIEKHYGCPMDIEWAKDG
DDGKLYIVQARPETVKSRTQANVMERYLLKETGTVLVEGRAIGQRIGAGKVRIIKDVSEMDKVQPGDVLVSDMTDPDWEP
VMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATQLLKDGQGVTVSCAEGDTGYIFEGELGFDIKKNSVDAMPELP
FKIMMNVGNPDRAFDFAQLPNAGVGLARLEFIINRMIGVHPKALLNYDGLPQDIKESVDKRIAGYDDPVGFYVEKLVEGI
STLAAAFAPKKVIVRLSDFKSNEYANLIGGKLYEPEEENPMLGFRGASRYISESFRDCFELECRALKRVRNEMGLTNVEI
MVPFVRTLGEASQVVDLLAENGLARGDNGLRVIMMCELPSNAILAEEFLEFFDGFSIGSNDLTQLTLGLDRDSGIIAHLF
DERNPAVKKLLANAIAACNKAGKYIGICGQGPSDHPDLAKWLMEQGIESVSLNPDTVLETWFFLAEGQAAE
>Mature_791_residues
MVEYVVSLDKLGKHDVEHVGGKNASLGEMISNLAGAGVSVPGGFATTAQAYRDFLELSGLNDQIHKALDALDVDDVNALA
KTGAQIRQWIMEAEFPEKLNTEIRTAFAALSAGNPDVAVAVRSSATAEDLPDASFAGQQETFLNIRGVENVIRAAKEVFA
SLFNDRAISYRVHQGFDHKLVALSAGVQRMVRSETGTAGVMFTLDTESGFRDVVFITGAYGLGETVVQGAVNPDEFYVHK
GTLEAGRPAILRRNLGSKAIKMIYGDEAKAGRSVKTVDVDKAERARFCLTDAEVSELAKQAMIIEKHYGCPMDIEWAKDG
DDGKLYIVQARPETVKSRTQANVMERYLLKETGTVLVEGRAIGQRIGAGKVRIIKDVSEMDKVQPGDVLVSDMTDPDWEP
VMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATQLLKDGQGVTVSCAEGDTGYIFEGELGFDIKKNSVDAMPELP
FKIMMNVGNPDRAFDFAQLPNAGVGLARLEFIINRMIGVHPKALLNYDGLPQDIKESVDKRIAGYDDPVGFYVEKLVEGI
STLAAAFAPKKVIVRLSDFKSNEYANLIGGKLYEPEEENPMLGFRGASRYISESFRDCFELECRALKRVRNEMGLTNVEI
MVPFVRTLGEASQVVDLLAENGLARGDNGLRVIMMCELPSNAILAEEFLEFFDGFSIGSNDLTQLTLGLDRDSGIIAHLF
DERNPAVKKLLANAIAACNKAGKYIGICGQGPSDHPDLAKWLMEQGIESVSLNPDTVLETWFFLAEGQAAE

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=782, Percent_Identity=69.0537084398977, Blast_Score=1144, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=408, Percent_Identity=25.9803921568627, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI48994992, Length=190, Percent_Identity=32.6315789473684, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI1788726, Length=191, Percent_Identity=30.8900523560209, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1789193, Length=410, Percent_Identity=25.1219512195122, Blast_Score=86, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17564524, Length=346, Percent_Identity=27.1676300578035, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 85926; Mature: 85926

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEYVVSLDKLGKHDVEHVGGKNASLGEMISNLAGAGVSVPGGFATTAQAYRDFLELSGL
CCCCEEEHHHHCCHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCC
NDQIHKALDALDVDDVNALAKTGAQIRQWIMEAEFPEKLNTEIRTAFAALSAGNPDVAVA
CHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEE
VRSSATAEDLPDASFAGQQETFLNIRGVENVIRAAKEVFASLFNDRAISYRVHQGFDHKL
EECCCCHHCCCCCCCCCCHHHEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCH
VALSAGVQRMVRSETGTAGVMFTLDTESGFRDVVFITGAYGLGETVVQGAVNPDEFYVHK
HHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEECCCCCHHHHHCCCCCCCEEEEC
GTLEAGRPAILRRNLGSKAIKMIYGDEAKAGRSVKTVDVDKAERARFCLTDAEVSELAKQ
CCCCCCCCHHHHHCCCCHHEEEEECCCCCCCCCEEEEECCCHHHHHEEECHHHHHHHHHH
AMIIEKHYGCPMDIEWAKDGDDGKLYIVQARPETVKSRTQANVMERYLLKETGTVLVEGR
HHHHHHCCCCCCCEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEECH
AIGQRIGAGKVRIIKDVSEMDKVQPGDVLVSDMTDPDWEPVMKRASAIVTNRGGRTCHAA
HHHHHCCCCCEEEHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHH
IIARELGIPAVVGCGNATQLLKDGQGVTVSCAEGDTGYIFEGELGFDIKKNSVDAMPELP
HHHHHCCCCEEEECCCHHHHHHCCCCCEEEECCCCCCEEEECCCCCEEECCCCCCCCCCC
FKIMMNVGNPDRAFDFAQLPNAGVGLARLEFIINRMIGVHPKALLNYDGLPQDIKESVDK
CEEEEECCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHH
RIAGYDDPVGFYVEKLVEGISTLAAAFAPKKVIVRLSDFKSNEYANLIGGKLYEPEEENP
HHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHCCCCCCCCCCCC
MLGFRGASRYISESFRDCFELECRALKRVRNEMGLTNVEIMVPFVRTLGEASQVVDLLAE
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHH
NGLARGDNGLRVIMMCELPSNAILAEEFLEFFDGFSIGSNDLTQLTLGLDRDSGIIAHLF
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEEEEE
DERNPAVKKLLANAIAACNKAGKYIGICGQGPSDHPDLAKWLMEQGIESVSLNPDTVLET
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHH
WFFLAEGQAAE
HHHHCCCCCCC
>Mature Secondary Structure
MVEYVVSLDKLGKHDVEHVGGKNASLGEMISNLAGAGVSVPGGFATTAQAYRDFLELSGL
CCCCEEEHHHHCCHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCC
NDQIHKALDALDVDDVNALAKTGAQIRQWIMEAEFPEKLNTEIRTAFAALSAGNPDVAVA
CHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEE
VRSSATAEDLPDASFAGQQETFLNIRGVENVIRAAKEVFASLFNDRAISYRVHQGFDHKL
EECCCCHHCCCCCCCCCCHHHEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCH
VALSAGVQRMVRSETGTAGVMFTLDTESGFRDVVFITGAYGLGETVVQGAVNPDEFYVHK
HHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEECCCCCHHHHHCCCCCCCEEEEC
GTLEAGRPAILRRNLGSKAIKMIYGDEAKAGRSVKTVDVDKAERARFCLTDAEVSELAKQ
CCCCCCCCHHHHHCCCCHHEEEEECCCCCCCCCEEEEECCCHHHHHEEECHHHHHHHHHH
AMIIEKHYGCPMDIEWAKDGDDGKLYIVQARPETVKSRTQANVMERYLLKETGTVLVEGR
HHHHHHCCCCCCCEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEECH
AIGQRIGAGKVRIIKDVSEMDKVQPGDVLVSDMTDPDWEPVMKRASAIVTNRGGRTCHAA
HHHHHCCCCCEEEHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHH
IIARELGIPAVVGCGNATQLLKDGQGVTVSCAEGDTGYIFEGELGFDIKKNSVDAMPELP
HHHHHCCCCEEEECCCHHHHHHCCCCCEEEECCCCCCEEEECCCCCEEECCCCCCCCCCC
FKIMMNVGNPDRAFDFAQLPNAGVGLARLEFIINRMIGVHPKALLNYDGLPQDIKESVDK
CEEEEECCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHH
RIAGYDDPVGFYVEKLVEGISTLAAAFAPKKVIVRLSDFKSNEYANLIGGKLYEPEEENP
HHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHCCCCCCCCCCCC
MLGFRGASRYISESFRDCFELECRALKRVRNEMGLTNVEIMVPFVRTLGEASQVVDLLAE
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHH
NGLARGDNGLRVIMMCELPSNAILAEEFLEFFDGFSIGSNDLTQLTLGLDRDSGIIAHLF
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEEEEE
DERNPAVKKLLANAIAACNKAGKYIGICGQGPSDHPDLAKWLMEQGIESVSLNPDTVLET
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHH
WFFLAEGQAAE
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]