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The map label for this gene is caiC [H]

Identifier: 77457955

GI number: 77457955

Start: 1920531

End: 1922369

Strand: Direct

Name: caiC [H]

Synonym: Pfl01_1728

Alternate gene names: 77457955

Gene position: 1920531-1922369 (Clockwise)

Preceding gene: 77457953

Following gene: 77457956

Centisome position: 29.83

GC content: 62.15

Gene sequence:

>1839_bases
ATGCGCCACGCGCCGAGCGACACGATTACCTGGGGCATGATGCTCCGCAAACTGCCGATGATTGCCAAAGCCATCCCGCG
GGTGGTGAAAGGCATGAAGGTGGCCAACGTCAAGGATCCGACCCAAACCTGTGGCCTGGGCTGGACATTCGAGCAAGCGA
CCCTGCGCAATCCCGAAGGCCCCGCGCTGCTGTCGGGCGAGGTGGTGCTGAGTTATTCACAGGTCAACCAGTGGGCCAAC
CGCATTGCCCACTATTTGATCGGCCAGGGTATCGGCAAGGGCGATGTGGTGGCGGTGTTCATCGAGAACCGCCCGGAACT
GCTGGTGACGATTCTGGCCCTGGCCAAGGTCGGCGCCGTCAGCGCGCTGCTCAATACCTCGCAGACCCGCGATACGCTGA
TCCACAGCATCAATCTGGTGACGCCGGCGGCGATTGTCGTCGGTGAGGAACTGCTGCCGGCATTTGCGGCGGTACGCGAG
CAAGTGTCGATCCCGGCAACGCGCGCCTGGTTCGTCGCCGATCAGGATACCTTCAGCCATCCGGGCATCGCGCCCGAGGG
CTACATCAACCTGATCAGCGCCAGTGCCGACGCCTCGGGCGACAACCCGGCCAGCAGCCGGCAGATTTTCTTCGACGACC
CCTGTTTCTACATCTACACCTCCGGCACCACCGGTTTGCCCAAGGCCGGCGTGTTCAAGCACGGCCGCTGGATGCGCAGT
TCGGCGAGCTTCGGCATGATCGCCCTCGACATGCGCCCGGACGACATCGTCTATTGCACCTTGCCGCTGTACCACGCCAC
CGGGCTTTGCGTGTGTTGGGGTTCGGCGGTTAATGGCGCTTCGGGTTTCGCGATTCGCCGCAAGTTCAGCGCCCGCCAGT
TCTGGAGCGACGTGCGCCGCTACCGCGCAACCACCATCGGTTACGTCGGCGAGTTGTGCCGTTATCTGGTGGATCAGCCG
CTCAGCGCCGACGACAGCCGTCACGACGTGCGCAAAATGATCGGCAACGGCCTGCGGCCCGGCGCGTGGGCCGAGTTCAA
GACCCGTTTCGGCGTGGATCACATTTGCGAGCTGTACGCGGCCAGCGACGGCAATATTGGCTTCACCAACATCCTCAATT
TCGACAACACCATCGGCTTCTCGCTGATGGCCTGGGAACTGGTCGCCTACGACCAGGACAGCGGCGAGCCGATTCGTGGC
GCTGACGGGTTCATGCGCAAGGTCGGTCGGGGCGAGCGAGGCCTGCTGCTGGCGCGGATCGACGACAAGGCGCCGCTGGA
CGGCTACACCGATCCGCAGAAAACCGCCAAAGTCGTGCTGCAGGACGTGTTCATCAAAGGGGATCGCTTTTTCAATACCG
GCGATCTGCTGCGCAACATCGGTTTTGGTCATGCACAGTTTGTCGACCGCCTCGGCGACACCTACCGCTGGAAGGGAGAG
AACGTCTCGACCACCGAGGTCGAGAACCTGCTGCTCCAACATCCGCACATTTCCGAAGCCGTGGCCTATGGTGTGGAAAT
CCCCAACACCAACGGTCGCGCCGGGATGGCGGCGATCACCCCGGCCGAGTCTTTGGCGACCCTGGATTTCGCCGAACTGT
TGGCCTTCGTCCGCCAGCGTATGCCGGCCTACGCGGTGCCGTTGTTCTTACGGGTCAAGGTGAAAATGGAAACCACCGGC
ACCTTCAAATACCAGAAGACGCGCCTGAAAAACGAAGGCTTCGATCCCCGCCAGACAGGCGATGACCCGATTTATGCCTG
GCTGCCAGGGACAGAAACCTACGTGCAGGTCTCGGATGGAATCCTTGCGGACATCCGGGCAGGCAAGTTCCGTTATTGA

Upstream 100 bases:

>100_bases
ATTTTCGGCCACGACCCCCGCTCGAGAAGCGCCGGTTGCCTCACCCGATTGCCCGACAATAAAAATCCGGCCTGCACGTC
AAGGTCGAACGGGAGCTGCC

Downstream 100 bases:

>100_bases
TATTGGCTATCGGCGGTCTCGGAAAAAAGGGGATGACAGCTATCGCCGCGCTCGGGAAACTATCGGCTTTCCGATCGACC
GAAAGTGGAGTTGCCCCATG

Product: long-chain-acyl-CoA synthetase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 612; Mature: 612

Protein sequence:

>612_residues
MRHAPSDTITWGMMLRKLPMIAKAIPRVVKGMKVANVKDPTQTCGLGWTFEQATLRNPEGPALLSGEVVLSYSQVNQWAN
RIAHYLIGQGIGKGDVVAVFIENRPELLVTILALAKVGAVSALLNTSQTRDTLIHSINLVTPAAIVVGEELLPAFAAVRE
QVSIPATRAWFVADQDTFSHPGIAPEGYINLISASADASGDNPASSRQIFFDDPCFYIYTSGTTGLPKAGVFKHGRWMRS
SASFGMIALDMRPDDIVYCTLPLYHATGLCVCWGSAVNGASGFAIRRKFSARQFWSDVRRYRATTIGYVGELCRYLVDQP
LSADDSRHDVRKMIGNGLRPGAWAEFKTRFGVDHICELYAASDGNIGFTNILNFDNTIGFSLMAWELVAYDQDSGEPIRG
ADGFMRKVGRGERGLLLARIDDKAPLDGYTDPQKTAKVVLQDVFIKGDRFFNTGDLLRNIGFGHAQFVDRLGDTYRWKGE
NVSTTEVENLLLQHPHISEAVAYGVEIPNTNGRAGMAAITPAESLATLDFAELLAFVRQRMPAYAVPLFLRVKVKMETTG
TFKYQKTRLKNEGFDPRQTGDDPIYAWLPGTETYVQVSDGILADIRAGKFRY

Sequences:

>Translated_612_residues
MRHAPSDTITWGMMLRKLPMIAKAIPRVVKGMKVANVKDPTQTCGLGWTFEQATLRNPEGPALLSGEVVLSYSQVNQWAN
RIAHYLIGQGIGKGDVVAVFIENRPELLVTILALAKVGAVSALLNTSQTRDTLIHSINLVTPAAIVVGEELLPAFAAVRE
QVSIPATRAWFVADQDTFSHPGIAPEGYINLISASADASGDNPASSRQIFFDDPCFYIYTSGTTGLPKAGVFKHGRWMRS
SASFGMIALDMRPDDIVYCTLPLYHATGLCVCWGSAVNGASGFAIRRKFSARQFWSDVRRYRATTIGYVGELCRYLVDQP
LSADDSRHDVRKMIGNGLRPGAWAEFKTRFGVDHICELYAASDGNIGFTNILNFDNTIGFSLMAWELVAYDQDSGEPIRG
ADGFMRKVGRGERGLLLARIDDKAPLDGYTDPQKTAKVVLQDVFIKGDRFFNTGDLLRNIGFGHAQFVDRLGDTYRWKGE
NVSTTEVENLLLQHPHISEAVAYGVEIPNTNGRAGMAAITPAESLATLDFAELLAFVRQRMPAYAVPLFLRVKVKMETTG
TFKYQKTRLKNEGFDPRQTGDDPIYAWLPGTETYVQVSDGILADIRAGKFRY
>Mature_612_residues
MRHAPSDTITWGMMLRKLPMIAKAIPRVVKGMKVANVKDPTQTCGLGWTFEQATLRNPEGPALLSGEVVLSYSQVNQWAN
RIAHYLIGQGIGKGDVVAVFIENRPELLVTILALAKVGAVSALLNTSQTRDTLIHSINLVTPAAIVVGEELLPAFAAVRE
QVSIPATRAWFVADQDTFSHPGIAPEGYINLISASADASGDNPASSRQIFFDDPCFYIYTSGTTGLPKAGVFKHGRWMRS
SASFGMIALDMRPDDIVYCTLPLYHATGLCVCWGSAVNGASGFAIRRKFSARQFWSDVRRYRATTIGYVGELCRYLVDQP
LSADDSRHDVRKMIGNGLRPGAWAEFKTRFGVDHICELYAASDGNIGFTNILNFDNTIGFSLMAWELVAYDQDSGEPIRG
ADGFMRKVGRGERGLLLARIDDKAPLDGYTDPQKTAKVVLQDVFIKGDRFFNTGDLLRNIGFGHAQFVDRLGDTYRWKGE
NVSTTEVENLLLQHPHISEAVAYGVEIPNTNGRAGMAAITPAESLATLDFAELLAFVRQRMPAYAVPLFLRVKVKMETTG
TFKYQKTRLKNEGFDPRQTGDDPIYAWLPGTETYVQVSDGILADIRAGKFRY

Specific function: Could catalyze the transfer of CoA to carnitine, generating the initial carnitinyl-CoA needed for the CaiB reaction cycle [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI40807357, Length=611, Percent_Identity=36.4975450081833, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI38524616, Length=577, Percent_Identity=37.4350086655113, Blast_Score=358, Evalue=8e-99,
Organism=Homo sapiens, GI62865631, Length=576, Percent_Identity=37.5, Blast_Score=357, Evalue=2e-98,
Organism=Homo sapiens, GI13325055, Length=576, Percent_Identity=37.5, Blast_Score=357, Evalue=2e-98,
Organism=Homo sapiens, GI227499619, Length=580, Percent_Identity=36.0344827586207, Blast_Score=344, Evalue=2e-94,
Organism=Homo sapiens, GI13236579, Length=531, Percent_Identity=36.9114877589454, Blast_Score=323, Evalue=3e-88,
Organism=Homo sapiens, GI13325057, Length=509, Percent_Identity=37.1316306483301, Blast_Score=293, Evalue=4e-79,
Organism=Homo sapiens, GI227499621, Length=570, Percent_Identity=33.5087719298246, Blast_Score=281, Evalue=2e-75,
Organism=Escherichia coli, GI221142682, Length=518, Percent_Identity=27.027027027027, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1788107, Length=562, Percent_Identity=21.7081850533808, Blast_Score=86, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17551278, Length=557, Percent_Identity=36.6247755834829, Blast_Score=346, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI115534440, Length=600, Percent_Identity=34.5, Blast_Score=328, Evalue=5e-90,
Organism=Saccharomyces cerevisiae, GI6319515, Length=518, Percent_Identity=31.4671814671815, Blast_Score=231, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6319699, Length=514, Percent_Identity=22.9571984435798, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281364798, Length=549, Percent_Identity=38.7978142076503, Blast_Score=359, Evalue=4e-99,
Organism=Drosophila melanogaster, GI24583465, Length=549, Percent_Identity=38.7978142076503, Blast_Score=358, Evalue=4e-99,
Organism=Drosophila melanogaster, GI24583463, Length=549, Percent_Identity=38.7978142076503, Blast_Score=358, Evalue=4e-99,
Organism=Drosophila melanogaster, GI20130353, Length=579, Percent_Identity=36.0967184801382, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI28573631, Length=579, Percent_Identity=36.0967184801382, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI45550496, Length=589, Percent_Identity=34.46519524618, Blast_Score=338, Evalue=7e-93,
Organism=Drosophila melanogaster, GI45552801, Length=589, Percent_Identity=34.46519524618, Blast_Score=338, Evalue=8e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 67432; Mature: 67432

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHAPSDTITWGMMLRKLPMIAKAIPRVVKGMKVANVKDPTQTCGLGWTFEQATLRNPEG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHCCCCEEECHHHCCCCCC
PALLSGEVVLSYSQVNQWANRIAHYLIGQGIGKGDVVAVFIENRPELLVTILALAKVGAV
CEEECCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
SALLNTSQTRDTLIHSINLVTPAAIVVGEELLPAFAAVREQVSIPATRAWFVADQDTFSH
HHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCC
PGIAPEGYINLISASADASGDNPASSRQIFFDDPCFYIYTSGTTGLPKAGVFKHGRWMRS
CCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCEEEEEECCCCCCCCCCCCCCCCEECC
SASFGMIALDMRPDDIVYCTLPLYHATGLCVCWGSAVNGASGFAIRRKFSARQFWSDVRR
CCCCCEEEEECCCCCEEEEEEHHHHHCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
YRATTIGYVGELCRYLVDQPLSADDSRHDVRKMIGNGLRPGAWAEFKTRFGVDHICELYA
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHC
ASDGNIGFTNILNFDNTIGFSLMAWELVAYDQDSGEPIRGADGFMRKVGRGERGLLLARI
CCCCCCCEEEEECCCCCCCHHHEEEEEEEEECCCCCCCCCCHHHHHHHCCCCCCEEEEEE
DDKAPLDGYTDPQKTAKVVLQDVFIKGDRFFNTGDLLRNIGFGHAQFVDRLGDTYRWKGE
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECHHHHHHHCCCCHHHHHHHCCCCEEECCC
NVSTTEVENLLLQHPHISEAVAYGVEIPNTNGRAGMAAITPAESLATLDFAELLAFVRQR
CCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHC
MPAYAVPLFLRVKVKMETTGTFKYQKTRLKNEGFDPRQTGDDPIYAWLPGTETYVQVSDG
CCHHHCCEEEEEEEEEECCCCEEEHHHHHCCCCCCCCCCCCCCEEEEECCCCEEEEECCC
ILADIRAGKFRY
EEEECCCCCCCC
>Mature Secondary Structure
MRHAPSDTITWGMMLRKLPMIAKAIPRVVKGMKVANVKDPTQTCGLGWTFEQATLRNPEG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHCCCCEEECHHHCCCCCC
PALLSGEVVLSYSQVNQWANRIAHYLIGQGIGKGDVVAVFIENRPELLVTILALAKVGAV
CEEECCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
SALLNTSQTRDTLIHSINLVTPAAIVVGEELLPAFAAVREQVSIPATRAWFVADQDTFSH
HHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCC
PGIAPEGYINLISASADASGDNPASSRQIFFDDPCFYIYTSGTTGLPKAGVFKHGRWMRS
CCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCEEEEEECCCCCCCCCCCCCCCCEECC
SASFGMIALDMRPDDIVYCTLPLYHATGLCVCWGSAVNGASGFAIRRKFSARQFWSDVRR
CCCCCEEEEECCCCCEEEEEEHHHHHCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
YRATTIGYVGELCRYLVDQPLSADDSRHDVRKMIGNGLRPGAWAEFKTRFGVDHICELYA
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHC
ASDGNIGFTNILNFDNTIGFSLMAWELVAYDQDSGEPIRGADGFMRKVGRGERGLLLARI
CCCCCCCEEEEECCCCCCCHHHEEEEEEEEECCCCCCCCCCHHHHHHHCCCCCCEEEEEE
DDKAPLDGYTDPQKTAKVVLQDVFIKGDRFFNTGDLLRNIGFGHAQFVDRLGDTYRWKGE
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECHHHHHHHCCCCHHHHHHHCCCCEEECCC
NVSTTEVENLLLQHPHISEAVAYGVEIPNTNGRAGMAAITPAESLATLDFAELLAFVRQR
CCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHC
MPAYAVPLFLRVKVKMETTGTFKYQKTRLKNEGFDPRQTGDDPIYAWLPGTETYVQVSDG
CCHHHCCEEEEEEEEEECCCCEEEHHHHHCCCCCCCCCCCCCCEEEEECCCCEEEEECCC
ILADIRAGKFRY
EEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA